The invention provides an SNP (
Single Nucleotide Polymorphism) site for detecting the carcass rate and the ocular
muscle area of cattle, the SNP site is located at the 48848th basic group of the cattle ACACB
gene and is located at the 63911805 position of the No.17
chromosome, and the
genotype of the SNP site is CC, CT or TT. Therefore, the SNP (
Single Nucleotide Polymorphism) is named as NC037344.1: g.48848Cgt; t. The invention also provides a KASP detection method for the carcass rate and the
eye muscle area of the cattle, which comprises the following steps of: extracting
DNA (Deoxyribonucleic Acid) of a cattle individual to be detected, then
typing an amplified fragment, and judging the carcass rate and the
eye muscle area level according to a
typing result. The method for detecting the carcass rate and the
eye muscle area of the cattle is obtained by researching the correlation between the ACACB and the carcass rate and the eye
muscle area, breeding of the cattle can be facilitated on the basis of the method, a foundation is laid for further improving the carcass rate of the cattle and conveniently and rapidly judging the condition of the eye
muscle area of the beef, and the method has important significance on breeding of the cattle with excellent meat quality.