MSC (mesenchymal stem cell) injection as well as preparation and application thereof

Abstract

The invention relates to the field of biology, in particular to MSC (mesenchymal stem cell) injection as well as a preparation and an application thereof. The injection comprises MSCs and a cell cryopreservation solution, wherein the cryopreservation solution comprises components in percentage by volume as follows: 25%-70% of an electrolyte balance solution, 5%-20% of clinical-grade DMSO (dimethyl sulfoxide), 1%-50% of 20% human serum albumin, 1%-10% of hydroxyethyl starch 130 / 0.4 and 5%-20% of triphosadenine-disodium magnesium chloride freeze-drying powder. The injection is free of animal serum, has clear ingredients and good cell cryopreservation effect and is safe and controllable, long-term storage and long-distance transport are facilitated, the survival rate of cells after recovery is higher than 95%, the vitality is high, and the injection can effectively relieve injury and inflammation symptoms of lesion tissue of lungs and promote tissue regeneration of the lungs, so that acute lung injury can be fundamentally and comprehensively treated.

Description

technical field

[0001] The invention relates to the field of biology, in particular to a mesenchymal stem cell injection, a preparation method and an application thereof. Background technique

[0002] In the 1960s, acute lung injury (ALI) and acute respiratory distress syndrome (ARDS) were recognized as a severe acute respiratory failure syndrome, mainly manifested as hypoxemia , Severe acute respiratory failure with bilateral lung infiltration, common in pneumonia, sepsis, and major trauma. The pathogenesis of ALI / ARDS involves damage to the pulmonary vascular endothelium, damage to the alveolar epithelium, and accumulation of protein-rich exudates and cellular debris in the alveolar spaces. The current treatment plan for ALI / ARDS is mainly supportive treatment, giving lung protective ventilation and conservative drug treatment, and a large number of therapeutic drugs evaluated in clinical trials so far, such as inhaled surfactants, NO, glucocorticoids, ketocon None of az...

Examples

Embodiment 1

[0053] Example 1: Preparation of umbilical cord mesenchymal stem cells

[0054] The preparation of umbilical cord mesenchymal stem cells comprises the following steps:

[0055] (1) Take fresh umbilical cords taken by cesarean section that have left the mother’s body for no more than 2 hours, and check whether the puerpera is carrying infectious disease pathogens (negative hepatitis B surface antigen, hepatitis C antibody, syphilis antibody, and AIDS antibody), and place the fresh umbilical cord containing D- In the special storage bottle of Hanks balance solution, it is transported in a low-temperature ice box;

[0056] (2) Put the umbilical cord in a biosafety cabinet for ultraviolet irradiation for 30 minutes, preheat the water-bath shaker at 37°C in advance, and place the umbilical cord sample bottle in the biosafety cabinet for operation. Take out the sample with long hemostatic forceps and place it in a 150mm sterile glass dish, and wash away the blood on the surface of ...

Embodiment 2

[0065] Example 2: Preparation of Umbilical Cord Mesenchymal Stem Cell Injection

[0066] Described cryopreservation solution comprises following components:

[0067]

[0068] (1) Take Bomaili A injection, 20% human serum albumin, hydroxyethyl starch 130 / 0.4 and adenosine triphosphate-disodium magnesium chloride freeze-dried powder according to the above formula, and mix them;

[0069] (2) Filter the mixed solution in step (1) with a 0.22 μm bacterial filter, then add the prepared umbilical cord mesenchymal stem cells, and the concentration is controlled at 5×10 6 cells / ml, cell suspension was obtained;

[0070] (3) Add clinical-grade DMSO to the cell suspension in step (2), and pack in In the cell cryopreservation bag, the mesenchymal stem cell injection was obtained.

Embodiment 3

[0071] Example 3: Preparation of Umbilical Cord Mesenchymal Stem Cell Injection

[0072]

[0073] The preparation method was as in Example 2, and the concentration of umbilical cord mesenchymal stem cells was controlled at 8×10 6 pieces / ml.

technical field

[0001] The invention relates to the field of biology, in particular to a mesenchymal stem cell injection, a preparation method and an application thereof. Background technique

[0002] In the 1960s, acute lung injury (ALI) and acute respiratory distress syndrome (ARDS) were recognized as a severe acute respiratory failure syndrome, mainly manifested as hypoxemia , Severe acute respiratory failure with bilateral lung infiltration, common in pneumonia, sepsis, and major trauma. The pathogenesis of ALI / ARDS involves damage to the pulmonary vascular endothelium, damage to the alveolar epithelium, and accumulation of protein-rich exudates and cellular debris in the alveolar spaces. The current treatment plan for ALI / ARDS is mainly supportive treatment, giving lung protective ventilation and conservative drug treatment, and a large number of therapeutic drugs evaluated in clinical trials so far, such as inhaled surfactants, NO, glucocorticoids, ketocon None of az...

Examples

Embodiment 1

[0053] Example 1: Preparation of umbilical cord mesenchymal stem cells

[0054] The preparation of umbilical cord mesenchymal stem cells comprises the following steps:

[0055] (1) Take fresh umbilical cords taken by cesarean section that have left the mother’s body for no more than 2 hours, and check whether the puerpera is carrying infectious disease pathogens (negative hepatitis B surface antigen, hepatitis C antibody, syphilis antibody, and AIDS antibody), and place the fresh umbilical cord containing D- In the special storage bottle of Hanks balance solution, it is transported in a low-temperature ice box;

[0056] (2) Put the umbilical cord in a biosafety cabinet for ultraviolet irradiation for 30 minutes, preheat the water-bath shaker at 37°C in advance, and place the umbilical cord sample bottle in the biosafety cabinet for operation. Take out the sample with long hemostatic forceps and place it in a 150mm sterile glass dish, and wash away the blood on the surface of ...

Embodiment 2

[0065] Example 2: Preparation of Umbilical Cord Mesenchymal Stem Cell Injection

[0066] Described cryopreservation solution comprises following components:

[0067]

[0068] (1) Take Bomaili A injection, 20% human serum albumin, hydroxyethyl starch 130 / 0.4 and adenosine triphosphate-disodium magnesium chloride freeze-dried powder according to the above formula, and mix them;

[0069] (2) Filter the mixed solution in step (1) with a 0.22 μm bacterial filter, then add the prepared umbilical cord mesenchymal stem cells, and the concentration is controlled at 5×10 6 cells / ml, cell suspension was obtained;

[0070] (3) Add clinical-grade DMSO to the cell suspension in step (2), and pack in In the cell cryopreservation bag, the mesenchymal stem cell injection was obtained.

Embodiment 3

[0071] Example 3: Preparation of Umbilical Cord Mesenchymal Stem Cell Injection

[0072]

[0073] The preparation method was as in Example 2, and the concentration of umbilical cord mesenchymal stem cells was controlled at 8×10 6 pieces / ml.

Images