43 results about "Cellular Debris" patented technology

The invention discloses a preparation method of artemisia apiacea extracellular vesicles, which comprises the following steps: step 1, pre-treating artemisia apiacea, carrying out primary centrifugal treatment, removing large plant tissues and cell debris, carrying out secondary centrifugal treatment, filtering by a needle filter, and collecting; step 2, transferring the filtered suspension to a sucrose density gradient solution, then carrying out third centrifugal treatment, collecting the solution with the concentration of 30% in the layer, and carrying out fourth centrifugal treatment to obtain artemisia apiacea extracellular vesicles; the invention further discloses an artemisia apiacea extracellular vesicle, a pharmaceutical composition for treating cerebral apoplexy, a medicine and application of the artemisia apiacea extracellular vesicle in preparation of the medicine for treating cerebral apoplexy. The naturally-sourced vesicle delivery system provided by the invention is expected to have higher safety and lower toxic and side effects, and the enrichment concentration of the therapeutic component at the focus part of cerebral apoplexy is improved, so that the bioavailability and the treatment efficiency of the medicine are remarkably improved.

The invention discloses an extraction method and application of purslane exosomes, belongs to the technical field of plant exosome extraction, and can improve the structural integrity, yield and purity of the purslane exosomes. Comprising the following steps: (1) cutting a weighed purslane sample into pieces, adding 90-120ml of a PBS buffer solution, and soaking overnight to obtain a soaking solution; (2) adding a proper amount of PBS (Phosphate Buffer Solution) into the soak solution, and then carrying out high-speed homogenization treatment for 8-10 times to obtain homogenate; (3) filtering large fragments in the homogenate to obtain a first supernatant; (4) sequentially carrying out gradient centrifugal treatment: centrifuging 1000g for 10 minutes to remove coarse particles, centrifuging 2000g for 20 minutes to remove cell debris, centrifuging 4000 g for 30 minutes to remove large-particle vesicles, and centrifuging 10000g for 60 minutes to remove impurities; (5) filtering the supernate obtained in the step (4) by using a sterile 0.22 mu m filter, and carrying out 120000g ultracentrifugation for 70min to obtain a precipitate; and (6) resuspending the precipitate by using a sterile PBS buffer solution to obtain a purslane exosome suspension.

The invention discloses an antibacterial peptide concentration and purification device, and particularly relates to the technical field of concentration and purification device.The antibacterial peptide concentration and purification device comprises a sewage tank and a liquid storage tank, a filtering part is fixed to the upper side of the sewage tank, a water storage tank is fixed to one side of the liquid storage tank, a first water pump is fixed to the upper side of the liquid storage tank, and a concentration part is fixed to the upper side of the water storage tank; a purification assembly is mounted on one side of the concentration part. According to the concentration and purification device for the antibacterial peptide, broken cell sap can be tangentially filtered through the arranged filtering parts, macromolecular substances such as cell debris and lipid are prevented from being deposited on the surface of a membrane, and the multiple filtering assemblies can be backwashed, so that the cell debris attached to the surface of the membrane in the filtering assemblies is reduced, and the concentration and purification efficiency of the antibacterial peptide is improved. The problem of frequent replacement caused by blockage of the filtering membrane is avoided, meanwhile, impurities in the filtered supernatant can be less, and the influence on the product quality is reduced.

The invention discloses a preparation method and application of recombinant human-like collagen. The recombinant human-like collagen is fusion protein of collagen and elastin. The preparation method comprises the following steps: taking constructed escherichia coli engineering bacteria, inducing expression of human-like collagen through high-density fermentation, performing high-pressure homogeneous cell disruption after tubular centrifugal collection of fermented thalli, performing acid regulation, flocculating and settling of cell debris and host protein, and controlling pH and conductivity of feed liquid to obtain the human-like collagen. Through ion exchange chromatography purification and ultrafiltration concentration replacement, a human-like collagen stock solution with the liquid phase purity of more than 98% is obtained, and the total yield is more than 75%. Through cell biological activity detection and analysis, the purified human-like collagen has remarkable biological activity of promoting cell migration and adhesion. The whole preparation process developed by the invention is simple and convenient in process, high in yield, easy to amplify and suitable for industrial production of the recombinant human-like collagen, and the prepared protein raw material can be widely applied to the fields of medical beauty and biological medicine.

The invention discloses an efficient purification process of a bispecific antibody and a special chromatography system thereof. The efficient purification process of the bispecific antibody comprises the following steps: pretreatment: performing 0.22 mu m microfiltration on a cell culture supernatant to remove cell debris, and performing deep filtration to reduce turbidity to be less than or equal to 1NTU so as to reduce subsequent chromatography medium pollution; carrying out improved Protein A affinity chromatography, namely carrying out affinity chromatography by adopting an agarose medium modified by a recombinant Protein A ligand, taking 20mM Tris-HCl as a loading buffer solution, and taking the pH value of the loading buffer solution as 7.4, taking 100mM citric acid as an elution buffer solution, and taking the pH value of the elution buffer solution as 3.2-3.5; performing mixed mode chromatography: adopting a composite mode medium containing a benzamidine functional group, taking 50mM sodium phosphate as a buffer solution, adjusting the pH value to 6.0-6.5, adjusting the NaCl concentration to 0.1-0.3 M, and specifically removing a double-antibody polymer and a mismatched isomer by utilizing a hydrophobic effect and an ion exchange synergistic effect; and performing nanoscale filtration and terminal treatment: removing viruses by adopting a virus filtration membrane with the aperture of 30nm, and performing 0.22 mu m sterile filtration to obtain a final product. According to the process, the purification efficiency, the yield and the product purity are remarkably improved, and the process is suitable for efficient purification of the bispecific antibody.

The utility model discloses a cell induced separation device, which belongs to the technical field of cell separation, and adopts the technical scheme that the cell induced separation device comprises a base, the top of the base is fixedly connected with a frame, and a first cleaning pool, a second cleaning pool and an operation table are sequentially arranged in the frame from left to right, and an ultrasonic generating device is arranged in the first cleaning pool; by arranging the flushing assembly, the flushing assembly can spray flushing fluid to the culture chamber from multiple angles, the surface of the culture chamber can be comprehensively and efficiently flushed, so that pollutants such as residual cell debris and an inducer are rapidly removed, the cleaning efficiency is greatly improved, and the cleanliness of the culture chamber is ensured; according to the cell culture device, the culture chamber can be accurately moved into the flushing assembly to be flushed, then moved into the first cleaning pool to be deeply cleaned and then moved to the operation table to be subjected to subsequent cell induction or separation operation, and the use convenience is greatly improved through the flexible position adjusting function.

This invention relates to a system for separating and purifying 1,3-propanediol from microbial fermentation broth and its application. The system comprises a microfiltration membrane module, an ultrafiltration membrane module, an electrodialysis unit, a nanofiltration membrane module, and a distillation unit connected in sequence. Based on membrane and distillation technologies, this system offers advantages such as high operational flexibility, small space requirement, ease of operation, and no additional waste generation. It ultimately removes microbial cells or cell debris from the fermentation broth, retains and separates proteins, polysaccharides, and nucleic acids, removes salts, and separates small-molecule organic acids and neutral solutes, thereby purifying and enriching 1,3-propanediol to obtain a 1,3-propanediol product with high purity.

The invention discloses an extraction method and application of extracellular vesicles in camellia oleifera leaf protoplast lotion. The method comprises the following steps: (1) taking complete disease-free camellia oleifera leaves, cutting off petioles, cleaning with ultrapure water, and absorbing water with filter paper; (2) putting the cleaned leaves into a needleless injector cylinder added with an infiltrating liquid, infiltrating in a vacuumizing manner, and centrifuging the infiltrated leaves to obtain a camellia oleifera leaf protoplast washing liquid; and (3) carrying out graded ultracentrifugation on the protoplast washing liquid, and collecting supernate. (4) filtering out cell debris from the supernate through a needle filter to obtain filtrate; (5) centrifuging the filtrate again, removing large vesicles and / or microparticle precipitates, and collecting supernate; (6) adding the supernatant into an ultracentrifuge tube for centrifugation, and removing the supernatant to obtain extracellular vesicle precipitate; and (7) gently resuspending the extracellular vesicle precipitate by using the precooled PBS, centrifuging the resuspended solution, and removing the supernatant to obtain the washed extracellular vesicle precipitate.

The present application relates to the technical field of platelet exosome separation, and discloses a platelet exosome multistage laminar flow separation device, which comprises a liquid storage tank, a sleeve frame is symmetrically sleeved outside the liquid storage tank, a laminar flow box for laminar flow is arranged below the liquid storage tank, a first separation cabin for realizing primary separation is arranged below the laminar flow box, a second separation cabin for realizing resonance separation is arranged below the first separation cabin, a shunt box for shunting is arranged below the second separation cabin, and a plurality of collection tubes are arranged below the shunt box in an array, the present application realizes stable rectification of a sample and sheath liquid through the laminar flow box, the inertial force and the Dean force are utilized to accurately grade microvesicles, exosomes and cell fragments in combination with the gradually deepening spiral groove structure of the conical core in the first separation cabin, and the second resonance separation is performed on the exosomes through the resonance screens with different amplitudes and phase differences arranged in the second separation cabin, so that the enrichment purity of the exosomes is significantly improved.

The invention relates to a high-titer influenza virus seed as well as a preparation method and application thereof, and the preparation method comprises the following steps: S1, obtaining a virus, inoculating the virus into a cell factory, and culturing to obtain a cell supernatant; and S2, carrying out microfiltration on the cell supernatant to remove cell debris in the cell supernatant, carrying out ultrafiltration concentration, and carrying out degerming filtration to obtain the high-titer influenza virus seed. According to the technical scheme, the cell factory is adopted for culturing the virus, the virus is inoculated to the ten-layer cell factory, after culturing, the cell supernatant in the cell factory is harvested, microfiltration is carried out, and the cell debris is removed, so that compared with a conventional method for removing the cell debris through centrifugation, microfiltration needs shorter time, the volume of the supernatant which can be treated at a time is larger, and the cell debris can be removed at a time. The sterile risk is lower; and concentrating the supernate, and sterilizing and filtering after concentration. The virus titer of the virus seed subjected to the preparation process can be increased by 5-10 times, and the impurity content is lower.

The present invention relates to Lactobacillus paracasei HH20 MXS_B2_71_3 strain DSM 34860 and / or Lactobacillus rhamnosus HH03 OB-Lb-0655 strain DSM 34859, in the form of viable cells and / or non-viable cells and / or cell fragments and / or as fermentation broth, or combinations thereof, for use as a medicament, preferably for use as a medicament in one or more human and / or animal subject; as well as oral care compositions, methods and kits comprising said species.

The application discloses a safe, efficient and low-cost cleaning solution for suspended cells and application thereof, which comprises multiple kinds of glucose, amino acid supplements, vitamin supplements, anti-shear protective agents and sodium bicarbonate, is designed for the harvesting and washing process of suspended cells, can effectively remove residual culture medium, cell fragments, metabolic waste and process-related impurities while effectively maintaining cell viability, viable cell density and normal morphology, so as to realize the harvesting target of high-purity, high-activity and low-risk material of suspended cells, and is especially suitable for the production process of high-demand cell products such as cell culture meat. The cleaning solution system can be adapted to the continuous harvesting process of a large-scale bioreactor, can be integrated with automatic harvesting equipment, realizes stable, efficient and repeatable cell collection and washing operation, and meets the full-scene requirements from laboratory research and development to industrial production.

The invention discloses a method for separating plasmid DNA through floating impurities in combination with an alkali lysis method, which comprises the following steps of: S1, lysing bacteria through the alkali lysis method, and releasing the plasmid DNA; s2, adding sodium bicarbonate into a product obtained in the step S1, and floating impurities; and S3, taking a lower-layer clear solution, and purifying to obtain plasmid DNA. According to the method disclosed by the invention, sodium bicarbonate is added into a product after alkali cracking to generate a large amount of gas, impurities such as cell debris and denatured protein generated by cracking can float on the surface of liquid within 3-10 minutes, and solid-liquid separation is simply and conveniently carried out, so that plasmid DNA is separated out, and expanded production is facilitated; the method does not need to use a centrifugal machine, is convenient for process amplification, can directly carry out subsequent steps without using a deep filter, reduces the production cost, shortens the test time, and achieves the purposes of reducing the cost and improving the efficiency.

The utility model discloses an exosome culture and collection device. The exosome culture and collection device comprises a culture assembly, a collection assembly and a purification assembly, according to the exosome collecting device, the culture bottle, the collecting bottle and the centrifugal tube are sequentially connected through the detachable connector, so that the operation of collecting exosomes is changed into semi-closed operation from original open operation, and the pollution risk in the collecting process can be effectively reduced; the third connector is connected with a negative pressure liquid suction pump, the third connector is connected with a negative pressure liquid suction pump, the third connector is connected with a negative pressure liquid suction pump, and the negative pressure liquid suction pump is connected with a negative pressure liquid suction pump. And after the negative pressure liquid suction pump is opened, liquid in the collection bottle can quickly enter the high-speed centrifugal tube through the micro-filtration membrane, so that bacteria can be effectively removed, cell fragments and protein fragments with smaller diameters can be filtered out, and the collection and purification efficiency is improved.

The utility model relates to the technical field of preparation device, specifically disclose a kind of preparation device of canine feline interference virus compound peptide, including base, motor is fixedly installed in base middle part, motor output shaft end portion is fixedly installed with disc, disc side edge is equipped with the evenly distributed limit mouth, disc is located in the limit mouth side edge and is all equipped with through -going hole, disc side edge, limit mouth and through -going hole are all set to be inclined, through -going hole is equipped with centrifugal tube, centrifugal tube is set to be through -going, the plug of centrifugal tube top is fixedly connected, the size of plug is compatible with the size of limit mouth, the preparation device of canine feline interference virus compound peptide, can quickly directly canine feline cell liquid solid-liquid separation, using centrifugal force quickly makes cell debris, solid impurities such as organelle sedimentation and isolation, efficiently obtain the clear solution containing target interference virus compound peptide, strong practicality.

The application discloses a kind of concentrated purification device of antibacterial peptide, more particularly to concentrated purification device technical field, including sewage tank and liquid storage tank, the sewage tank upside is fixed with filter part, one side of the liquid storage tank is fixed with water storage tank, the liquid storage tank upside is fixed with water pump one, the water storage tank upside is fixed with concentration part, the concentration part one side is equipped with purification assembly.The concentrated purification device of antibacterial peptide described in the application can carry out tangential filtration to broken cell liquid by the filter part, avoid the deposition of macromolecular substances such as cell debris and lipids on the surface of the membrane, and the multiple filter assemblies can also be back-flushed, thereby reducing the cell debris attached to the surface of the membrane in the filter assembly, avoiding the problem of frequent replacement due to clogging of the filter membrane, and at the same time, the impurities in the filtered supernatant are less, reducing the impact on product quality.

The invention discloses an extraction method and application of membranous small vesicles. The extraction method comprises the following steps: collecting a biological sample, and selecting at least one of blood, urine and cell culture supernatant; centrifuging the sample at 10,000 xg for 20 minutes, and removing cell debris and apoptotic bodies to obtain a pretreated sample; adding 20% by volume of sample separation liquid and CD63 / CD81 antibody modified magnetic beads into the pretreated sample, and incubating and capturing target vesicles; freezing the captured vesicles with liquid nitrogen for 15-35 minutes, and dissolving the vesicles with a 33-43 DEG C water bath; and cracking the dissolved vesicles with 0.5 W / cm < 2 > ultrasonic waves to finally obtain the intact vesicles of 30-70 nm and soluble protein factors. Original plasma or urine can be directly treated, dilution is not needed, and loss of a target object is avoided; a low-cost and degradable biomimetic polymer is developed to replace an imported kit, and the cost is reduced by 75%; the whole process lt; the time is 2.5 hours, which is superior to that of an ultracentrifugation method; and the extracted membranous small vesicles have good integrity and are suitable for liquid biopsy and drug carrier construction.

The present application belongs to the technical field of quality control materials. Disclosed are a quality control material freeze-dried powder as well as a preparation method therefor and the use thereof. The quality control material freeze-dried powder is obtained by freeze-drying cell pellets containing cell fragments, the cell pellets being obtained by sectioning, deparaffinizing and hydrating a paraffin-embedded cell sample and then centrifuging same. The quality control material freeze-dried powder prepared by using the preparation method in the present application can reduce the loss of cell fragments during the freeze-drying process, so as to ensure the high recovery rate of cells, can avoid performance change or protein loss of cell fragments during the freeze-drying process, and can ensure that the immunohistochemical staining performance of reconstituted cell pellets after freeze-drying remains unchanged. The quality control material freeze-dried powder obtained by using the preparation method in the present application has extended storage time and facilitates transport, and the reconstituted cell suspension has good dispersibility, retains key target substances, and exhibits normal immunohistochemical staining performance.

The invention relates to the technical field of cell extraction, in particular to an extraction method of rabbit brain tissue single-cell suspension and a kit. The kit comprises 100 to 1000 U / mL of collagenase IV, 200 to 2000 U / mL of collagenase II, 300 to 700 U / mL of DNA (Deoxyribonucleic Acid) enzyme I, 1 to 10 mg / mL of vitamin E polyethylene glycol succinate and 100 to 1000 U / mL of EDTA (Ethylene Diamine Tetraacetic Acid) pancreatin. The kit further comprises a cell debris removal buffer solution, and the cell debris removal buffer solution comprises a low-density buffer solution and a high-density buffer solution. The kit can be particularly used for extracting single-cell suspension from rabbit brain tissues; the single-cell suspension is low in caking rate, large in number of cells, high in nucleation rate and large in cell grouping, red blood cells almost do not exist in the single-cell suspension, and subsequent researches such as sequencing are facilitated; the time for preparing the single-cell suspension is short, and the pretreatment is simple.

The utility model provides a verticillium dahliae secreted protein generating and collecting device, which relates to the technical field of verticillium dahliae secreted protein and comprises an operating table, a drying machine is arranged at the top of the operating table, a filter component is arranged at the position, located at the lower end of the drying machine, of the operating table, and the filter component comprises a filter box. A handle is connected to the front surface of the filter box, inserting grooves are formed in the outer surfaces of the two sides of the handle, a fixing box is connected to the surface, close to the handle, of the operation table, a two-way screw is inserted into the fixing box, and the two ends of the two-way screw are connected with a bearing and a handle correspondingly; and moving blocks are connected to the surfaces of the two sides of the two-way screw rod. According to the verticillium dahliae secretory protein drying device, after verticillium dahliae secretory protein is dried, impurities can be filtered through the filter box, so that the problem that subsequent biological functions and application effects of the dried secretory protein are affected due to the fact that the dried secretory protein contains cell debris or other impurities which are not removed is solved.

A process for determining the sensitivity or resistance of at least one identified bacterium to at least one antibiotic, wherein the process includes the following steps: a) bringing a sample including bacterium into contact with the at least one antibiotic, the antibiotic inducing the rupture of the bacterial wall and / or of the cytoplasmic membrane and causing the release of the intracellular compounds of the bacterium when the bacterium is termed “sensitive” to the at least one antibiotic, b) incubating the sample with the at least one antibiotic, c) purifying the sample by removing the intact bacteria and the cell debris, d) analyzing the purified sample by mass spectrometry, e) detecting the presence or absence of at least one peak of at least one protein characteristic of the bacterium, and f) determining the sensitivity or the resistance of the bacterial population to the antibiotic.

The present invention provides a novel process for the preparation of a extracellular vesicle (EV) comprising composition, the process comprising the steps of (i) growing stem cells in a culture medium; possibly dissociating the stem cells obtained in step (i) from their support and possibly separating the stem cells from the medium used in step (i); (ii) rinsing the obtained stem cells in an isotonic electrolyte solution; (iii) subjecting the cell containing isotonic electrolyte solution to a gentle mechanical stimulation and separating the cells from the medium and retaining the medium, the separated cells being discarded; and (iv) possibly separating remaining undesirable components, cells and cell debris from the medium of interest retained at step (iii) The discarded cells may be used in further culture cycles and / or for other applications. The EV comprising composition of the invention further comprises cell- originating immunomodulating proteins and miRNAs and may be used in the treatment of disorders selected from Systemic Inflammation Response Syndrome (SIRS); Acute Respiratory Distress Syndrome (ARDS), acute or degenerative conditions of several organs as liver, lung, heart, uterus, brain, eye, skin, bone, tendon and cartilage, in mammalian subjects.

The invention belongs to the technical field of biomedical materials, and particularly relates to a clinical ready-to-use autologous adipose tissue-derived matrix collagen membrane and application thereof. The clinical ready-to-use autologous adipose tissue-derived matrix collagen membrane provided by the invention is mainly composed of autologous adipose tissue-derived collagen, and contains a plurality of key adhesion proteins, glycosaminoglycans, growth factors and other bioactive components. The preparation method comprises the following steps: by taking autologous adipose tissue as a raw material, breaking cells by a mechanical method, repeatedly cleaning to remove cell components such as cell debris and oil drops, and mechanically pressing through a mold to form a diaphragm with uniform thickness, regular shape and rough surface. The membrane effectively overcomes the limitation of the existing acellular matrix biological membrane, can load various bioactive substances to enhance the function, can be stored at low temperature for a long time without influencing the repair effect, and greatly enhances the convenience of subsequent use.

The invention relates to the field of biology, in particular to a proliferation culture method of high-titer mouse parvovirus. The method comprises the following steps: centrifuging mouse parvovirus harvest liquid to remove cell debris, taking supernatant, filtering with a filter membrane to remove bacteria, carrying out ultrafiltration concentration on a sample to be concentrated, and then carrying out conventional multiplication culture to obtain high-titer mouse parvovirus liquid. Compared with a traditional'conventional mouse parvovirus multiplication-concentration experiment 'mode, the multiplication culture method has the advantages that the yield of the viruses cannot be reduced, the quantity of the obtained viruses is large, the operation is simple, and the cost is low.

This invention relates to the field of cell image analysis and biological cell screening technology, and discloses a method for screening liver stem cells. The method includes: acquiring original images; performing effective image screening; performing background correction, noise reduction and enhancement, and contrast compensation; performing single-cell instance segmentation; performing single-cell feature extraction and trajectory association; determining target cells using a neural network model; and outputting the liver stem cell determination result. Compared to existing screening methods that rely on manual observation or single-frame static image discrimination, especially in the case of primary hepatocyte suspensions containing mature hepatocytes, bile duct epithelial cells, stromal cells, and cell debris, this method addresses the technical problem of unstable and automated identification of liver stem cells. This application improves the accuracy and automation of liver stem cell screening by constructing a continuous processing flow of image quality gating, instance segmentation, and dual-branch feature fusion judgment.

The utility model relates to the technical field of medical teaching, and discloses a teaching auxiliary device for paclitaxel drug-resistant breast cancer cell exosome separation, which comprises L-shaped supporting legs, an operation table, a supporting vertical plate A and a supporting vertical plate B, a supporting vertical plate A is mounted at one end of the top of the operating table through screws, a horizontal top plate is welded to one side of the top end of the supporting vertical plate A, a filter-pressing electric cylinder is mounted at the bottom of the horizontal top plate through a mounting seat, and a piston plate is mounted on an output shaft of the filter-pressing electric cylinder through screws; according to the teaching auxiliary device for separating the taxol drug-resistant breast cancer cell exosome, cell debris and large-particle substances can be conveniently removed in advance and rapidly cleaned, the cell exosome and tissue fluid are automatically collected, subsequent centrifugal operation is facilitated, a sample is heated to a proper temperature, and the subsequent cell exosome separation effect is improved.

Analysis of cell populations for both research and clinical applications such as cell therapy is often performed on whole blood that is close to 24 hours old. The reason for this is that the analysis site is removed from the blood draw site so that blood must be shipped overnight to the analysis site. During the 24-hour shipping period, granulocytes and other blood components break down into undesirable cell debris including nucleic acids that are released into the blood. Such debris is known to interfere with immunological assays and with thawing following freezing of samples. The present invention solves this issue by removing granulocytes (and optionally other components) using an anti-CD15 molecule (or other appropriate molecule) bound to metallic magnetic particles. Following mixing, the granulocytes (or other components) are removed and prepared for shipment to the analysis site or for other reasons.

The invention discloses an exosome as well as a preparation method and application thereof, and relates to the technical field of exosomes. Comprising the following steps: (1) normally culturing human lung stem cells in a serum-free culture system, and collecting cell culture supernatant; (2) carrying out differential centrifugation on the collected culture supernatant to remove cell debris, mixing the culture supernatant with an exosome extraction reagent according to a ratio of 4: 1, standing overnight, centrifuging on the second day, discarding the supernatant, and precipitating to obtain an exosome sample; and (3) re-suspending the exosome sample by using PBS. According to the method for preparing the exosome from the human lung stem cells, differential centrifugation and ultrafiltration concentration are combined, meanwhile, an exosome extraction reagent is used for precipitating the exosome, finally, the exosome is obtained, the method is convenient and rapid, and a large number of exosomes can be extracted at a time.

The present invention relates to a method for producing and purifying a viral vector (V), wherein the viral vector (V) is produced by cells in a fluid (F), and the fluid (F) containing the viral vector (V) is guided through a tangential flow filtration device (2) for purification, thereby purifying the viral vector (V) in the fluid (F). It is proposed that cells and cellular debris contained in the fluid (F) containing the viral vector (V) after producing the viral vector (V) are at least partially separated from the viral vector (V) by a fluidized bed centrifuge (5) before purification by the tangential flow filtration device (2).