26 results about "Cellular Debris" patented technology

The invention discloses a preparation method of artemisia apiacea extracellular vesicles, which comprises the following steps: step 1, pre-treating artemisia apiacea, carrying out primary centrifugal treatment, removing large plant tissues and cell debris, carrying out secondary centrifugal treatment, filtering by a needle filter, and collecting; step 2, transferring the filtered suspension to a sucrose density gradient solution, then carrying out third centrifugal treatment, collecting the solution with the concentration of 30% in the layer, and carrying out fourth centrifugal treatment to obtain artemisia apiacea extracellular vesicles; the invention further discloses an artemisia apiacea extracellular vesicle, a pharmaceutical composition for treating cerebral apoplexy, a medicine and application of the artemisia apiacea extracellular vesicle in preparation of the medicine for treating cerebral apoplexy. The naturally-sourced vesicle delivery system provided by the invention is expected to have higher safety and lower toxic and side effects, and the enrichment concentration of the therapeutic component at the focus part of cerebral apoplexy is improved, so that the bioavailability and the treatment efficiency of the medicine are remarkably improved.

The utility model discloses a cell induced separation device, which belongs to the technical field of cell separation, and adopts the technical scheme that the cell induced separation device comprises a base, the top of the base is fixedly connected with a frame, and a first cleaning pool, a second cleaning pool and an operation table are sequentially arranged in the frame from left to right, and an ultrasonic generating device is arranged in the first cleaning pool; by arranging the flushing assembly, the flushing assembly can spray flushing fluid to the culture chamber from multiple angles, the surface of the culture chamber can be comprehensively and efficiently flushed, so that pollutants such as residual cell debris and an inducer are rapidly removed, the cleaning efficiency is greatly improved, and the cleanliness of the culture chamber is ensured; according to the cell culture device, the culture chamber can be accurately moved into the flushing assembly to be flushed, then moved into the first cleaning pool to be deeply cleaned and then moved to the operation table to be subjected to subsequent cell induction or separation operation, and the use convenience is greatly improved through the flexible position adjusting function.

This invention relates to a system for separating and purifying 1,3-propanediol from microbial fermentation broth and its application. The system comprises a microfiltration membrane module, an ultrafiltration membrane module, an electrodialysis unit, a nanofiltration membrane module, and a distillation unit connected in sequence. Based on membrane and distillation technologies, this system offers advantages such as high operational flexibility, small space requirement, ease of operation, and no additional waste generation. It ultimately removes microbial cells or cell debris from the fermentation broth, retains and separates proteins, polysaccharides, and nucleic acids, removes salts, and separates small-molecule organic acids and neutral solutes, thereby purifying and enriching 1,3-propanediol to obtain a 1,3-propanediol product with high purity.

The present application relates to the technical field of platelet exosome separation, and discloses a platelet exosome multistage laminar flow separation device, which comprises a liquid storage tank, a sleeve frame is symmetrically sleeved outside the liquid storage tank, a laminar flow box for laminar flow is arranged below the liquid storage tank, a first separation cabin for realizing primary separation is arranged below the laminar flow box, a second separation cabin for realizing resonance separation is arranged below the first separation cabin, a shunt box for shunting is arranged below the second separation cabin, and a plurality of collection tubes are arranged below the shunt box in an array, the present application realizes stable rectification of a sample and sheath liquid through the laminar flow box, the inertial force and the Dean force are utilized to accurately grade microvesicles, exosomes and cell fragments in combination with the gradually deepening spiral groove structure of the conical core in the first separation cabin, and the second resonance separation is performed on the exosomes through the resonance screens with different amplitudes and phase differences arranged in the second separation cabin, so that the enrichment purity of the exosomes is significantly improved.

The application discloses a safe, efficient and low-cost cleaning solution for suspended cells and application thereof, which comprises multiple kinds of glucose, amino acid supplements, vitamin supplements, anti-shear protective agents and sodium bicarbonate, is designed for the harvesting and washing process of suspended cells, can effectively remove residual culture medium, cell fragments, metabolic waste and process-related impurities while effectively maintaining cell viability, viable cell density and normal morphology, so as to realize the harvesting target of high-purity, high-activity and low-risk material of suspended cells, and is especially suitable for the production process of high-demand cell products such as cell culture meat. The cleaning solution system can be adapted to the continuous harvesting process of a large-scale bioreactor, can be integrated with automatic harvesting equipment, realizes stable, efficient and repeatable cell collection and washing operation, and meets the full-scene requirements from laboratory research and development to industrial production.

The utility model relates to the technical field of preparation device, specifically disclose a kind of preparation device of canine feline interference virus compound peptide, including base, motor is fixedly installed in base middle part, motor output shaft end portion is fixedly installed with disc, disc side edge is equipped with the evenly distributed limit mouth, disc is located in the limit mouth side edge and is all equipped with through -going hole, disc side edge, limit mouth and through -going hole are all set to be inclined, through -going hole is equipped with centrifugal tube, centrifugal tube is set to be through -going, the plug of centrifugal tube top is fixedly connected, the size of plug is compatible with the size of limit mouth, the preparation device of canine feline interference virus compound peptide, can quickly directly canine feline cell liquid solid-liquid separation, using centrifugal force quickly makes cell debris, solid impurities such as organelle sedimentation and isolation, efficiently obtain the clear solution containing target interference virus compound peptide, strong practicality.

The application discloses a kind of concentrated purification device of antibacterial peptide, more particularly to concentrated purification device technical field, including sewage tank and liquid storage tank, the sewage tank upside is fixed with filter part, one side of the liquid storage tank is fixed with water storage tank, the liquid storage tank upside is fixed with water pump one, the water storage tank upside is fixed with concentration part, the concentration part one side is equipped with purification assembly.The concentrated purification device of antibacterial peptide described in the application can carry out tangential filtration to broken cell liquid by the filter part, avoid the deposition of macromolecular substances such as cell debris and lipids on the surface of the membrane, and the multiple filter assemblies can also be back-flushed, thereby reducing the cell debris attached to the surface of the membrane in the filter assembly, avoiding the problem of frequent replacement due to clogging of the filter membrane, and at the same time, the impurities in the filtered supernatant are less, reducing the impact on product quality.

The invention discloses an extraction method and application of membranous small vesicles. The extraction method comprises the following steps: collecting a biological sample, and selecting at least one of blood, urine and cell culture supernatant; centrifuging the sample at 10,000 xg for 20 minutes, and removing cell debris and apoptotic bodies to obtain a pretreated sample; adding 20% by volume of sample separation liquid and CD63 / CD81 antibody modified magnetic beads into the pretreated sample, and incubating and capturing target vesicles; freezing the captured vesicles with liquid nitrogen for 15-35 minutes, and dissolving the vesicles with a 33-43 DEG C water bath; and cracking the dissolved vesicles with 0.5 W / cm < 2 > ultrasonic waves to finally obtain the intact vesicles of 30-70 nm and soluble protein factors. Original plasma or urine can be directly treated, dilution is not needed, and loss of a target object is avoided; a low-cost and degradable biomimetic polymer is developed to replace an imported kit, and the cost is reduced by 75%; the whole process lt; the time is 2.5 hours, which is superior to that of an ultracentrifugation method; and the extracted membranous small vesicles have good integrity and are suitable for liquid biopsy and drug carrier construction.

The utility model provides a verticillium dahliae secreted protein generating and collecting device, which relates to the technical field of verticillium dahliae secreted protein and comprises an operating table, a drying machine is arranged at the top of the operating table, a filter component is arranged at the position, located at the lower end of the drying machine, of the operating table, and the filter component comprises a filter box. A handle is connected to the front surface of the filter box, inserting grooves are formed in the outer surfaces of the two sides of the handle, a fixing box is connected to the surface, close to the handle, of the operation table, a two-way screw is inserted into the fixing box, and the two ends of the two-way screw are connected with a bearing and a handle correspondingly; and moving blocks are connected to the surfaces of the two sides of the two-way screw rod. According to the verticillium dahliae secretory protein drying device, after verticillium dahliae secretory protein is dried, impurities can be filtered through the filter box, so that the problem that subsequent biological functions and application effects of the dried secretory protein are affected due to the fact that the dried secretory protein contains cell debris or other impurities which are not removed is solved.

The invention belongs to the technical field of biomedical materials, and particularly relates to a clinical ready-to-use autologous adipose tissue-derived matrix collagen membrane and application thereof. The clinical ready-to-use autologous adipose tissue-derived matrix collagen membrane provided by the invention is mainly composed of autologous adipose tissue-derived collagen, and contains a plurality of key adhesion proteins, glycosaminoglycans, growth factors and other bioactive components. The preparation method comprises the following steps: by taking autologous adipose tissue as a raw material, breaking cells by a mechanical method, repeatedly cleaning to remove cell components such as cell debris and oil drops, and mechanically pressing through a mold to form a diaphragm with uniform thickness, regular shape and rough surface. The membrane effectively overcomes the limitation of the existing acellular matrix biological membrane, can load various bioactive substances to enhance the function, can be stored at low temperature for a long time without influencing the repair effect, and greatly enhances the convenience of subsequent use.

The invention relates to the field of biology, in particular to a proliferation culture method of high-titer mouse parvovirus. The method comprises the following steps: centrifuging mouse parvovirus harvest liquid to remove cell debris, taking supernatant, filtering with a filter membrane to remove bacteria, carrying out ultrafiltration concentration on a sample to be concentrated, and then carrying out conventional multiplication culture to obtain high-titer mouse parvovirus liquid. Compared with a traditional'conventional mouse parvovirus multiplication-concentration experiment 'mode, the multiplication culture method has the advantages that the yield of the viruses cannot be reduced, the quantity of the obtained viruses is large, the operation is simple, and the cost is low.

This invention relates to the field of cell image analysis and biological cell screening technology, and discloses a method for screening liver stem cells. The method includes: acquiring original images; performing effective image screening; performing background correction, noise reduction and enhancement, and contrast compensation; performing single-cell instance segmentation; performing single-cell feature extraction and trajectory association; determining target cells using a neural network model; and outputting the liver stem cell determination result. Compared to existing screening methods that rely on manual observation or single-frame static image discrimination, especially in the case of primary hepatocyte suspensions containing mature hepatocytes, bile duct epithelial cells, stromal cells, and cell debris, this method addresses the technical problem of unstable and automated identification of liver stem cells. This application improves the accuracy and automation of liver stem cell screening by constructing a continuous processing flow of image quality gating, instance segmentation, and dual-branch feature fusion judgment.

Analysis of cell populations for both research and clinical applications such as cell therapy is often performed on whole blood that is close to 24 hours old. The reason for this is that the analysis site is removed from the blood draw site so that blood must be shipped overnight to the analysis site. During the 24-hour shipping period, granulocytes and other blood components break down into undesirable cell debris including nucleic acids that are released into the blood. Such debris is known to interfere with immunological assays and with thawing following freezing of samples. The present invention solves this issue by removing granulocytes (and optionally other components) using an anti-CD15 molecule (or other appropriate molecule) bound to metallic magnetic particles. Following mixing, the granulocytes (or other components) are removed and prepared for shipment to the analysis site or for other reasons.

The present invention relates to a method for producing and purifying a viral vector (V), wherein the viral vector (V) is produced by cells in a fluid (F), and the fluid (F) containing the viral vector (V) is guided through a tangential flow filtration device (2) for purification, thereby purifying the viral vector (V) in the fluid (F). It is proposed that cells and cellular debris contained in the fluid (F) containing the viral vector (V) after producing the viral vector (V) are at least partially separated from the viral vector (V) by a fluidized bed centrifuge (5) before purification by the tangential flow filtration device (2).

The invention provides an exosome extraction method and prepared exosome freeze-dried powder, and belongs to the technical field of exosome extraction. The invention provides an exosome extraction method which comprises the following steps: firstly, performing high-temperature stimulation on cells to improve secretion and release of exosomes; then the blood platelets subjected to high-temperature stimulation are subjected to circulating freeze thawing, so that most of cells, vesicles and the like are broken, and the integrity of the target product exosome is reserved; and filtering to remove impurities such as cell debris, apoptotic bodies and the like, and retaining an exosome product of 50-150nm. The invention also provides exosome freeze-dried powder which is prepared by freeze-drying the exosome product, and the purity of the exosome freeze-dried powder can reach 70-80%. By utilizing the exosome extraction method disclosed by the invention, the capacity of the exosome can be remarkably improved, and the method disclosed by the invention can be used for removing most impurities and retaining the integrity and activity of the exosome, so that the purity of the prepared exosome product is improved.

The embodiment of the invention provides an enzyme-based tissue dissociation method which comprises the following steps: sequentially carrying out first digestion, second digestion and third digestion on a tissue by using a first enzyme, a second enzyme and a third enzyme so as to dissociate the tissue, the second enzyme is a mixed enzyme and comprises a mixed enzyme of collagenase I and collagenase IV; the third enzyme comprises trypsin. According to the embodiment of the invention, the enzyme-based tissue dissociation method which is low in cost, high in dissociation efficiency, short in treatment time and few in generated cell debris impurities is successfully established for the first time, and the human scalp tissue single-cell suspension with complete cell types can be obtained; and good technical support is provided for single-cell-based researches such as single-cell sequencing, single-cell apparent group research and the like.

This invention discloses a method for preparing purslane exosomes with anti-allergic effects and their applications, specifically relating to the field of natural active substances. The method includes washing fresh purslane and then juicing or homogenizing it, separating solid residue from the liquid juice to obtain a plant homogenate supernatant as a precursor. The precursor is then subjected to low-temperature differential centrifugation to remove cell debris and large particulate impurities, and the clarified supernatant is collected. The clarified supernatant is then filtered through one or more multi-stage filter membranes, with pore sizes selected from 5 μm, 1.2 μm, 0.45 μm, 0.22 μm, and 100 nm. Through optimized extraction processes, such as specific enzymatic hydrolysis steps, centrifugation conditions, and ultrafiltration technology, the extraction efficiency and purity of exosome-like nanovesicles are significantly improved. Compared to traditional extraction methods, this method can more accurately remove impurities, preserve the integrity and bioactivity of exosome-like nanovesicles, and ensure their stability and effectiveness in subsequent applications.

The invention discloses a preparation BMSCs-sec for repairing scar uterus and a preparation method thereof.The preparation method comprises the steps that primary BMSCs growing well are subcultured to the third generation, and BMSC cells are collected; the method comprises the following steps: inoculating BMSCs into a culture bottle, culturing in a complete culture medium, when the cell adhesion fusion rate reaches 80%, discarding the supernatant, cleaning cells with PBS, adding DMEM / F12, culturing, collecting the cell supernatant, centrifuging, removing cell debris and dead cells, filtering with a filter, and collecting the filtrate; and transferring the filtrate into an ultrafiltration tube, and centrifuging to obtain a concentrated cell supernatant, thereby obtaining the preparation BMSCs-sec. The prepared preparation BMSCs-sec is used for repairing the scar uterus, and a new thought is provided for treating the uterus scar injury by using cell secretions.

The invention discloses an extraction method and application of extracellular vesicles in camellia oleifera leaf protoplast lotion. The method comprises the following steps: (1) taking complete disease-free camellia oleifera leaves, cutting off petioles, cleaning with ultrapure water, and absorbing water with filter paper; (2) putting the cleaned leaves into a needleless injector cylinder added with an infiltrating liquid, infiltrating in a vacuumizing manner, and centrifuging the infiltrated leaves to obtain a camellia oleifera leaf protoplast washing liquid; and (3) carrying out graded ultracentrifugation on the protoplast washing liquid, and collecting supernate. (4) filtering out cell debris from the supernate through a needle filter to obtain filtrate; (5) centrifuging the filtrate again, removing large vesicles and / or microparticle precipitates, and collecting supernate; (6) adding the supernatant into an ultracentrifuge tube for centrifugation, and removing the supernatant to obtain extracellular vesicle precipitate; and (7) gently resuspending the extracellular vesicle precipitate by using the precooled PBS, centrifuging the resuspended solution, and removing the supernatant to obtain the washed extracellular vesicle precipitate.

Disclosed are methods for identifying the presence or absence of a target nucleic acid from one or more organisms in a biological sample, the methods comprising: (a) spinning the sample at a spin speed sufficient to precipitate cellular debris and fluorescence inhibitors present in the sample, reducing fluorescence interference or quenching in the sample; and (b) directly amplifying and detecting the target nucleic acid in the sample.

The invention discloses a preparation method of a hypoallergenic acellular matrix implant, and belongs to the technical field of biomedical materials. The preparation method comprises the following steps: swelling animal soft tissues in an alkaline solution for a short time, quickly freezing and crushing; placing the crushed sample in a hypertonic-hypotonic solution system, and performing efficient and mild decellularization treatment through circulating oscillation; then, endotoxin and other sensitizers are specifically removed by adopting a chromatography technology in sequence, denatured protein is washed by utilizing a salt solution, and finally, purification is performed through ultrafiltration or dialysis. According to the method, cell debris, nucleic acid and endotoxin can be deeply removed through multi-step synergy and combination of hypertonic-hypotonic circulating decellularization and chromatographic purification, the immunogenicity of an implant is remarkably reduced, and the natural three-dimensional structure and biological activity of an extracellular matrix are reserved to the maximum extent. The implant prepared by the method has the characteristics of low DNA residual quantity, extremely low endotoxin content and excellent biocompatibility, and is suitable for soft tissue repair and regeneration.

The utility model provides a dog and cat stem cell exosome extraction device, and belongs to the technical field of stem cells. The dog and cat stem cell exosome extraction device comprises a transparent mixing tank and a filtering barrel, a rotating part is arranged at an opening of the filtering barrel, the mixing tank rotates on the rotating part, a communicating pipe is arranged in the mixing tank in a penetrating and lifting mode, an overflow opening is formed in the middle of the communicating pipe in a penetrating mode, and the overflow opening is communicated with the filtering barrel. And the mixing tank is conditionally communicated with the filtering barrel through the communicating pipe. The exosome extraction device has the following effects that the overflow port sinks to a proper depth of a solution through the ascending and descending of the communicating pipe, and a supernatant rich in exosomes passes through the communicating pipe from the overflow port and enters the filtering barrel below for filtering, so that impurities such as cell debris and dead cells participating in filtering are reduced, the extraction effect is improved, and the extraction efficiency is improved. And the maintenance frequency of the filter barrel is also reduced.

Analysis of cell populations for both research and clinical applications such as cell therapy is often performed on whole blood that is close to 24 hours old. The reason for this is that the analysis site is removed from the blood draw site so that blood must be shipped overnight to the analysis site. During the 24-hour shipping period granulocytes, primarily neutrophils, breakdown into cell debris including release of nucleic acids into the blood. Such debris is known to interfere with immunological assays and with rethawing following freezing of samples. There is a need for a method and apparatus to solve this issue. The invention disclosed herein solves the issue. Following blood draw at the draw site the neutrophils are removed using an apparatus that draws blood into a tube that contains anti-CD15 bound to nickel magnetic particles. Following mixing the tube is placed in a magnetic field and then while still in the magnetic field the blood with neutrophils removed is transferred into a new tube for shipment to the analysis site.

The invention relates to the technical field of medicine research and development, in particular to a preparation method of stem cell exosomes and application of the stem cell exosomes in skin aging deficit, and the preparation method comprises the following steps: S1, taking clean umbilical cord tissues, and placing the clean umbilical cord tissues in a culture medium; the method comprises the following steps: uniformly spreading sheared tissue blocks in culture dishes, adding a culture medium into each dish, continuously filling oxygen into the culture medium in the culture process, collecting cell culture supernate, pretreating a sample to remove cell debris and large-particle substances, centrifuging cells, and storing at-80 DEG C, so as to obtain the cell culture medium. 1 mg of exosome is extracted and fully mixed with 10 ml of normal saline, the stem cell exosome injection is prepared for standby application, under the condition that the skin structure is not damaged, the stem cell exosome is injected into the deep layer of the skin through a microneedle, the absorption rate of the exosome is increased, the anti-aging effect is enhanced, the stem cell exosome can promote skin proliferation, and the anti-aging effect is improved. The stem cell exosome can regulate and control cell migration, and the stem cell exosome can influence cell apoptosis.

The present invention relates to a method for producing fermented extracellular vesicles, the method comprising a sterilization step. The method for producing fermented extracellular vesicles, according to the present invention, comprises: a fermentation step of obtaining a fermented product using microorganisms; and a sterilization step of separating a first material containing extracellular vesicles and impurities from a biological solution obtained by sterilizing the fermented product to thereby obtain the first material. The present invention can provide a method for producing fermented extracellular vesicles, which can efficiently and economically separate, from a biological solution obtained by sterilizing a microbial fermentation product, impurities such as microorganisms, bacteria, cell debris, wastes, proteins, and large particles, extracellular vesicles, and / or exosomes.