43 results about "Depth filter" patented technology

The present invention provides a method for producing extracellular vesicles, the method including a step for passing a crushed liquid of plants, mushrooms, or microalgae through a depth filter, and a step for subjecting the crushed liquid after passing through the depth filter to ultrafiltration treatment, the depth filter being composed of filaments.

The invention relates to the technical field of cast-in-place pile bottom sediment cleaning, and provides an intelligent cast-in-place pile bottom sediment cleaning system and method based on air pressure difference driving, and the system comprises a movable motor, a movable air compressor, a small bridge crane, a control computer, and a pile bottom sediment intelligent cleaning device. The intelligent pile bottom sediment cleaning device comprises a sediment storage bin, a sediment suction pipe, a pneumatic valve, a water level detector, an in-bin optical fiber visual sensor, a primary filter screen, a fine filter screen, a deep filter screen, a supporting frame and a pile bottom sediment thickness detection device. According to the principle of internal and external air pressure difference, pile bottom sediment is sucked into the bin for filtering by reducing air pressure in the device, the device is small in environmental pollution, high in efficiency, independent of slurry performance and high in informatization and automation degree, the thickness of the pile bottom sediment of the cast-in-place pile can be accurately detected in the cleaning process, and the device is suitable for sediment of various media; the influence of factors such as sediment transparency is avoided.

The present invention discloses a method for improving the stability of solid-phase synthesized multi-cysteine ​​polypeptides, primarily addressing the technical problem of poor stability caused by the susceptibility of such polypeptides to oxidation, degradation, elimination, denaturation, and inactivation. The method comprises the following steps: 1) purifying the multi-cysteine ​​polypeptide synthesized by solid-phase synthesis via liquid chromatography to obtain a preparation solution containing trifluoroacetate; 2) passing the preparation solution through a depth filter containing hydrophilic silica aerogel spheres as a filter material to remove metal ions and trace peroxides contained in the preparation solution by adsorption; 3) further passing the preparation solution through a weakly basic cation exchange resin to a pH of 6.3-6.5; 4) removing the sodium trifluoroacetate in the preparation solution by tangential flow filtration with low pressure, obtaining a weakly acidic, salt-free preparation solution; 5) freezing and vacuum drying to obtain a solid powder final product; 6) vacuum packaging and frozen storage; and 7) ensuring purity and stability after storage in accordance with scientific research requirements.

A method for determining a deep filter, comprising the steps of: receiving a mixture; estimating the deep filter using a deep neural network, wherein the estimation is performed so that the deep filter, when applied to elements of the mixture, obtains an estimate of each element of a desired representation; wherein the deep filter having at least one dimension comprises a tensor having elements.

A method and system for purifying oil, the method comprising the steps of: - providing a separation agent and oil to be purified to at least one settling tank; - waiting to allow a sludge phase to settle to the bottom portion of the settling tank, the sludge phase comprising the separation agent along with impurities from the oil; - removing an oil phase excluding the sludge phase from at least one settling tank; and - filtering the oil phase through a depth filter to remove any possible residual separation agent and impurities.

The present disclosure provides purification platforms comprising a depth filter step and / or a hydrophobic interaction chromatography (HIC) step. Also disclosed herein are methods of using the purification platforms described herein and compositions obtained therefrom, such as pharmaceutical compositions.

The present invention relates to a method for isolating and enriching pathogenic nucleic acids from a biological sample thereby reducing host nucleic acid contamination and enrich relative pathogenic DNA in the infectious disease sample and a device thereof. The method involves mixing pathogenic nucleic acid containing biological sample with a lysis buffer at a temperature between 25-35°C for partial or incomplete lysis of the sample; passing the lysed sample through a depth filter wherein the filter paper size ranges from 0.22 microns to 20 microns; passing an elution buffer by reverse plunging action and generating a vibration of 200 to 350 Hz with discontinuous pulse of 20-30 seconds for 2-5 minutes for eluting pathogenic nucleic acids into the elution buffer. The invention further provides a device for isolating and enriching pathogenic nucleic acids from a nucleic acid containing biological sample thereby reducing host nucleic acid contamination using said method.

1. Name of the product in this design: Depth Filter. 2. Purpose of this design: for fluid filtration, separation, and purification. 3. The key design feature of this product is its shape. 4. The image or photograph that best illustrates the design's key points: a 3D model.

Provided herein are improved methods of purifying an Fc-containing protein from a mixture comprising an Fc-containing protein and a contaminant (e.g., an undesired host cell protein). The methods generally include heating the mixture to prepare a heat-treated mixture, and applying the heat-treated mixture to a depth filter to prepare a filtrate comprising Fc-containing proteins.

The wastewater treatment device comprises a pretreatment mechanism, a multifunctional treatment tank and a deep filtration tank, the pretreatment mechanism is communicated with the inner bottom of the multifunctional treatment tank through a first water outlet pipeline, and a Fe-Cu-C reaction layer, a catalytic oxidation filler layer and a first aeration mechanism which are distributed up and down are arranged in the multifunctional treatment tank; the Fe-Cu-C reaction layer and the catalytic oxidation filler layer are distributed above a water outlet of the first water outlet pipeline; the multifunctional treatment tank is communicated with the bottom of the deep filtering tank through a second water outlet pipeline, a filtering layer and a second aeration mechanism are arranged in the deep filtering tank, and the filtering layer is distributed above a water outlet of the second water outlet pipeline; the first aeration mechanism is connected with the ozone generator, and the second aeration mechanism is connected with the air generator. The device can reduce the energy consumption of wastewater treatment, meets the low-carbon requirement, occupies a small area, generates less sludge, and reduces the operation cost.

The invention discloses a method for separating plasmid DNA through floating impurities in combination with an alkali lysis method, which comprises the following steps of: S1, lysing bacteria through the alkali lysis method, and releasing the plasmid DNA; s2, adding sodium bicarbonate into a product obtained in the step S1, and floating impurities; and S3, taking a lower-layer clear solution, and purifying to obtain plasmid DNA. According to the method disclosed by the invention, sodium bicarbonate is added into a product after alkali cracking to generate a large amount of gas, impurities such as cell debris and denatured protein generated by cracking can float on the surface of liquid within 3-10 minutes, and solid-liquid separation is simply and conveniently carried out, so that plasmid DNA is separated out, and expanded production is facilitated; the method does not need to use a centrifugal machine, is convenient for process amplification, can directly carry out subsequent steps without using a deep filter, reduces the production cost, shortens the test time, and achieves the purposes of reducing the cost and improving the efficiency.

According to the preparation method of the hydrophobic deep filtering paperboard, natural fibers are subjected to hydrophobic modification, so that the deep filtering paperboard is endowed with uniform hydrophobic performance, the interaction between the surface of the paperboard and a filter cake layer can be reduced, and a filter cake is convenient to peel off. The hydrophobic natural fiber can be uniformly dispersed in water without additional treatment on the hydrophobic fiber, so that the hydrophobic fiber does not need to be subjected to pre-hydrophilic treatment in the paperboard preparation process, the process is simple, and the cost is low. The low-melting-point fibers are used as a binder, surface fibers of the deep filtering paperboard can be reinforced, when the deep filtering paperboard scrapes a filter cake, the surface fibers cannot fall off, the deep filtering paperboard has great wet strength and bending strength, and compared with a traditional deep filtering paperboard, the deep filtering paperboard has the advantages that the surface fibers are not prone to falling off; and when a filter cake is scraped, the filter cake can be prevented from being damaged due to bending and twisting in a wet state. The filter press disclosed by the invention is low in cost and convenient to use, and has a relatively high practical value.

The present invention relates to a filter medium being at least formed of a pre-filter substrate laminated with a fine-filter substrate by means of a third binder, wherein the pre-filter substrate comprises synthetic fibers and a first binder, the pre-filter substrate working as a combined surface and depth filter, and the fine-filter substrate comprises at least a second binder and one of synthetic fibers and inorganic fibers.

This invention provides an improved depth filtration medium with reduced extractant for DSP filtration and clarification applications. Furthermore, it provides an improved depth filtration medium that is compatible with gamma irradiation-based filter sterilization processes. [Solution] A depth filtration medium is provided for clarifying the recovered material from a feed stream of a cell culture containing therapeutic biomolecules. The depth filtration medium is composed of a sintered mixture of a polymer adsorbent and a thermoplastic binder, has extremely low extractability, and does not require pre-washing before use. Furthermore, the constituent materials used in the sintered depth filter medium exhibit high stability to gamma rays and are compatible with gamma-based pre-use sterilization processes.

Filter device for a liquid medium, in particular for water, comprising a hollow support body (6) with passages (14) for the liquid medium, wherein a first filter (8) is assigned to the hollow support body (6), wherein the first filter (8) is designed as a surface filter, wherein the filter device has a backwash device (11) in order to remove impurities from the first filter (8), characterized in that a second filter (9) is assigned to the support body (6), wherein the second filter (9) is designed as a depth filter and wherein the second filter has a porous body (17) made of a sintered plastic material.

1. Name of the Design Product: Filter (Deep Filter). 2. Use of the Design Product: For clarification filtration in biopharmaceutical processes. 3. Design Key Points of the Design Product: Lies in the shape. 4. Picture or Photograph that Best Illustrates the Design Key Points: Perspective view.

The application discloses a sea depth prediction method and system based on a variational filtering method and a baseband removal method, and a storage medium, relates to the technical field of geographic information data processing, and comprises the following steps: obtaining a gravity anomaly residual frequency domain signal and a ship-surveyed sea depth residual frequency domain signal; performing filter design to obtain a sea depth filter and a gravity anomaly filter; performing filtering to obtain a ship-surveyed sea depth mid-wave frequency domain signal and a gravity anomaly mid-wave frequency domain signal; obtaining a target area mid-wave predicted sea depth, a target area long-wave predicted sea depth and a target area short-wave predicted sea depth; and obtaining a target area predicted sea depth. In the scheme, the existing discrete data is used to obtain the target area predicted sea depth from the signal decomposition angle based on the semi-physical set and the semi-data fusion driving mode, the existing data is constrained in a fuzzy and wide range, the application range is widened, the experience interference is reduced, the time consumption is reduced while the result convergence is ensured, and the sea depth prediction effect is good.

The present invention provides a method for clarifying a cell culture, lysate or supernatant comprising AAV3 vector particles, the method comprising: (a) providing a cell culture, lysate or supernatant comprising AAV3 vector particles; and (b) filtering the cell culture, lysate or supernatant through a depth filter to provide a clarified cell culture, lysate or supernatant, wherein the depth filter comprises a first filtration medium comprising an anion exchange nonwoven substrate comprising a plurality of quaternary ammonium groups.

The invention provides a method for clarifying an adenovirus harvest liquid, which comprises deep filtration, the deep filtration comprises salt injection treatment on a feed liquid: a positive salt ion solution is added into the virus harvest liquid subjected to pyrolysis and enzymolysis, and the final concentration of the positive salt ion solution is 200-600mmol / L. The deep filtering specifically comprises the following steps: assembling a deep filter and a pipeline: connecting the deep filter to a proper pipeline, and installing a pressure gauge or a pressure sensor at the liquid inlet end of the deep filter; the deep filter is washed with purified water and a Tris-HCl buffer solution; performing salt injection treatment on the feed liquid; filtering the feed liquid, and detecting that the pressure at the liquid inlet end of a deep filter does not exceed 1bar; and carrying out top washing by using a Tris-HCl buffer solution to obtain 1-2 deep filter cavity volumes. According to the method, the recovery rate of the deep filtering process can be increased to 80% or above, and the loading capacity of the deep filter is also increased to some extent.

The utility model discloses filter cloth for sewage treatment, which comprises a main frame, one side of the main frame is of an open structure and is connected with a blocking frame, one end of the blocking frame is hinged with the main frame, the other end of the blocking frame is detachably connected with the main frame through a limiting assembly, and two pairs of auxiliary frames are longitudinally arranged in the main frame at intervals. Two pairs of auxiliary frames are longitudinally arranged in the main frame at intervals, coarse filter cloth, fine filter cloth, deep filter cloth and functional filter cloth are respectively arranged in the two pairs of auxiliary frames from top to bottom, and caulking grooves allowing the auxiliary frames to be embedded in a sliding mode are formed in the inner walls of the two sides of the main frame. The coarse filter cloth, the fine filter cloth, the deep filter cloth and the functional filter cloth are respectively mounted, so that multi-layer and all-directional efficient filtration of sewage is realized, and the sewage treatment effect is greatly improved.

The utility model discloses an azotometer for feed detection, and relates to the technical field of feed detection. The device comprises a reaction tank and a gas path assembly assembled at the top of the reaction tank, the reaction tank comprises a tank body and a filtering assembly, and the filtering assembly comprises a positioning ring attached to the annular edge of the top of the tank body, a primary filtering cylinder fixed to the edge of the inner ring of the positioning ring and a deep filtering cylinder coaxially fixed to the edge of the outer ring of the positioning ring. The gas circuit assembly comprises an annular exhaust grid, an annular baffle rotationally installed in the annular exhaust grid in a matched mode, a distribution base installed on the inner ring of the annular exhaust grid in a threaded matched mode, and a gas inlet pipe and a gas outlet pipe which are connected to the peripheral side of the distribution base. Through the design of the multi-stage filtering assembly, efficient impurity removal is achieved, the exhaust port of the annular exhaust grating and the annular baffle are arranged in a matched mode, flexible control over gas exhaust is achieved, the annular exhaust grating and the exhaust pipe are arranged in a distinguished mode, and nitrogen-containing gas can be conveniently and differentially collected.

The utility model discloses a gilding dust removal device based on improvement of printing quality, and belongs to the technical field of gilding dust removal for improvement of printing quality, the gilding dust removal device comprises a dust removal frame, an exhaust mechanism is installed in the dust removal frame, the exhaust mechanism comprises an outer shell, fan blades and a driving motor, and the fan blades are located in the outer shell; a driving motor is installed on one side of the fan blades, a preliminary filter screen is installed on one side of the air exhaust mechanism, a deep filtering frame is installed on one side of the preliminary filter screen, a non-woven fabric filter cotton layer, an activated carbon filter layer and a synthetic fiber filter cotton layer are sequentially arranged in the deep filtering frame, and an air outlet frame is installed at one end of the dust removal frame; an adjusting valve is mounted in the air outlet frame; when the dust removal device is used, the dust removal efficiency can be remarkably improved, 99% or above of tiny dust particles can be effectively removed while the structural integrity of paper is not damaged, the structure is compact and reasonable, maintenance is easy, convenient and rapid, and the operation cost input of an enterprise can be reduced.

The utility model discloses a movable container type sprout vegetable planting house, including the planting house body, the planting frame is arranged in the planting house body, the planting house body one end is provided with through -hole, the fan is fixedly installed in the through -hole inboard, and the through -hole is provided with depth filter screen in, the through -hole inside symmetry is provided with the slot, and the slot is fixedly installed with locking shell in, depth filter screen one end symmetry fixed mounting has inserted rod, and inserted rod is matched with the slot, the utility model discloses through pressing depth filter screen and realizes quick unlocking, and inserted rod drives trapezoidal block extrusion elastic locking block, makes locking rod exit limit slot, and unfastens the reset spring of pressing and pushes the sliding block back and moves, lets elastic locking block and trapezoidal block separate, and it can draw out inserted rod to complete disassembly to pull outwards, the whole process does not need tool, only through pressing and pulling operation can realize the quick disassembly of depth filter screen, is convenient for washing or replacement, improves maintenance convenience, guarantees filter screen filtering effect.

The invention discloses a sea depth prediction method and system based on a mixture of a variational filtering method and a baseband removal method, and a storage medium, and relates to the technical field of geographic information data processing, and the method comprises the steps: obtaining a gravity anomaly residual frequency domain signal and a ship-side sea depth residual frequency domain signal; performing filter design to obtain a sea depth filter and a gravity anomaly filter; filtering is carried out to obtain a ship survey sea depth medium wave frequency domain signal and a gravity anomaly medium wave frequency domain signal; obtaining a target area medium wave prediction sea depth, a target area long wave prediction sea depth and a target area short wave prediction sea depth; and obtaining the predicted sea depth of the target area. In the scheme of the invention, a semi-physical set and semi-data fusion driving mode is adopted to obtain the predicted sea depth of the target area from the perspective of signal decomposition based on the existing discrete data, and fuzzy and wide-range existing data constraints are adopted, so that the application range is widened, the experience interference is reduced, the time consumption is reduced while the result convergence is ensured, and the prediction efficiency is improved. And the sea depth prediction effect is good.

The present invention is to depth filter media that are suitable for the clarification of cell culture media streams used for viral and gene therapy (VGT) applications and that also have compatibility with radiation based sterilization methods.

A tubular depth filter element has one or more concentric zones. Each zone includes layers of a melt blown filament. An additional filament oscillates through the depth of the element, traversing through part of the depth of the filament with each oscillation but traversing through substantially all of the depth of the element over multiple oscillations. The traversing filament is preferably biased towards the outside of the element. The depth filter element may be made by spraying filaments onto a rotating mandrel. The filaments of the concentric zones are sprayed from fixed nozzles spaced apart along the length of the mandrel. The traversing filament is sprayed from a nozzle assembly that moves laterally or has compound motion relative to the mandrel. For example, the nozzle assembly may oscillate relative to the mandrel while an air attenuator of the nozzle assembly oscillates relative to the remainder of the nozzle assembly.

The utility model relates to waste oil filter assembly field, concretely relates to a kind of waste oil filter assembly of multilayer composite structure, including filter bucket, for waste oil filtration;Mounting cover is threadedly connected in the upper portion of filter bucket.The utility model relates to a kind of waste oil filter assembly of multilayer composite structure, through the setting of filter assembly, driving motor, transmission rod, stirring vane, first spur gear, second spur gear, gear ring and scraper, waste oil is transported to filter assembly inside by oil inlet, then driving motor switch is opened, driving transmission rod and stirring vane rotation, waste oil is stirred and rapidly filtered, when waste oil is filtered from pre-filter core, depth filter core, adsorption filter core and fine filter core in turn, and is discharged from the oil discharge port in the bottom of filter bucket, and in order to avoid causing blockage, when transmission rod rotates, the first spur gear outside is driven to drive second spur gear and gear ring rotation, and when gear ring rotates, scraper between each filter core is driven to rotate, so that each layer filter core is cleaned, avoid causing blockage.

To provide a method for producing extracellular vesicles derived from plants, mushrooms, or microalgae. [Solution] A method for producing extracellular vesicles, comprising the steps of passing a crushed liquid of plants, mushrooms, or microalgae through a depth filter, and ultrafiltration of the crushed liquid after passing through the depth filter, wherein the depth filter is composed of filaments.

The invention relates to a filter device for a liquid medium, in particular for water, comprising a hollow supporting body (6) having passages (14) for the liquid medium, wherein: a first filter (8) is associated with the hollow supporting body (6); the first filter (8) is designed as a surface filter; the filter device has a backwash device (11) in order to remove impurities from the first filter (8); a second filter (9) is associated with the supporting body (6); the second filter (9) is designed as a depth filter; and the second filter has a porous body (17) made of a sintered plastics material.

The present invention relates to hollow fiber tangential flow depth filtration media suitable for perfusion biological treatment, and related TFDF filtration units, biological treatment systems, and methods, including methods for perfusion of cell cultures. The hollow fiber elements of the present invention combine relatively thin porous walls with relatively low porosity to provide a filter media that resists fouling during perfusion of a culture.