The invention relates to a method for reinforcing a bridge structure separated steel-concrete combined truss. The method is characterized by comprising the following steps of: 1, processing an interface at the joint of upper and lower flanges of a concrete beam; 2, planting steel bars in concrete at the joint of the upper and lower flanges of the concrete beam; 3, manufacturing steel plates (10),low-height steel box girders (12) and welding studs (9); 4, binding steel bar meshes (11), and installing in place; 5, manufacturing and installing upper and lower chord node plates (17) and connecting members thereof; and coating a structural adhesive on pouring joint surfaces of the steel plates (10) and the low-height steel box girders (12), and scattering fine sand; 6, installing the steel plates (10) and the low-height steel box girders (12) of the upper and low flanges, vertical rods (16), an external cable (14) and an anchoring tool; 7, manufacturing and installing truss diagonal web rods (15); 8, constructing a truss support; 9, mixing, spraying and curing self-compacting concrete; and 10, tensioning the external cable (14) to obtain an external prestressed steel-concrete combinedtruss reinforced structure. The method has the advantages that: vehicle operation is not influenced, external prestressed bars can be replaced and repeatedly tensioned, a beam body has superior overall working performance and high durability, the process is simple, the combined truss is convenient to maintain and cure, and the like.