98 results about "Cell yield" patented technology

Methods for the maximizing parameter of the in vitro growth and expansion of mammalian cells, specifically postpartum-derived cells in containers such as roller bottles is described. Methods of optimizing growth rate and cell yield in such culture systems are provided. The methods are particularly adapted for human postpartum-derived cells, such as umbilicus-derived cells.

The invention relates to the preparation and application method of a medium, which is specifically related to a medium which is used for Vero cell and a culture method of the Vero cell. The invention belongs to the biological product area. The culture method of the invention is that the adhering Vero cells are suspend and cultured in SFM serum-free medium for the domestication and adaptation; the growing pattern of the Vero cell changes from adherence growth to suspension growth; the Vero cells are further amplified in Vero amplification CHEMICALLY DEFINED MEDIUM. The method of the invention has good effects; the growing pattern of the Vero cell is transformed from adherence growth to suspension growth, which changes the growth pattern of the cell and greatly increases the yield of the cell.

The invention relates to a culture method for efficiently obtaining adipose mesenchymal stem cells, and solves the problem that the cell viability obtained in the prior art is low. The culture method comprises the following steps: separation of the adipose mesenchymal stem cells: washing D.Hanks with fat tissues, detecting to guarantee no pollution, adding a solution mixture of I-type collagenase and trypLETM digestive enzyme with several times of volume, and stopping enzymolysis after carrying out rotary vibration digestion for dozens of minutes at the temperature of 37 DEG C, centrifuging to remove a liquid supernatant, and filtering with screen cloth to obtain an adipose mesenchymal stem cell suspension; culture of the adipose mesenchymal stem cells: inoculating a culture bottle bloodless culture medium with the adipose mesenchymal stem cell suspension, carrying out primary cell culture, observing with an inverted phase contrast microscope in the culture period, and changing a fresh culture medium, when the cells are cultured to 80% to 90% fusion, extensively inoculating a new culture bottle with the cells for culture after digestion with trypLETM, and subculturing for several generations to obtain the purified mesenchymal stem cells. The culture method has the advantages that the digestion is more sufficient, the cell yield is higher, the mesenchymal stem cells are protected from being damaged, and the viability is high.

The invention provides a method for producing viral vaccines, which comprises the following steps of: 1) inoculating cells for producing virus into a bioreactor with a Disks carrier, and culturing; and 2) harvesting the virus to prepare the vaccines. In addition, besides the step 1) of culturing the cells for producing the virus, the method further comprises the following steps of: when the cellsfor producing the virus grow to a certain density, inoculating and proliferating the virus to be produced. By the method, the cell yield can be improved, the production cost can be reduced, and the production efficiency can be improved.

The application discloses a preparation method of a fish single-cell suspension. The preparation method comprises the following steps: preparing a PBS reagent, a D-Han liquid and an IV type collagenase liquid, preparing a kidney tissue block of a fish, and washing the kidney tissue for more than three times with PBS; putting the kidney tissue block in a flat vessel of an ice bath, adding PBS, shearing the kidney tissue block into small pieces, and putting the tissue blocks in a high throughput tissue centrifuge tube of a burnisher; adding the IV type collagenase liquid into the centrifuge tube and meanwhile, adding PBS to fix the volume to 4ml, putting a zirconium oxide wall to grind, and performing enzymatic digestion on a tissue homogenate; and performing filtration, centrifugalization, washing with PBS, performing centrifugalization to abandon supernate and removing cell debris, filtering the cell suspension and fixing the volume to 3ml with PBS, and storing the cell suspension for later use at 4 DEG C. By adopting an enzymatic hydrolysis grinding method, advantages of a griding method and an enzymatic hydrolysis method are combined. The tissue is more fully treated by enzymatic hydrolysis grinding, so that the problem that the cell yield is low and the cell viability is low for preparing the fish single-cell suspension can be solved.

The present invention discloses a method of making cell growth surface, which comprises a three-dimensional porous growth surface made from polysaccharide material, especially the alginic acid, to enhance cell growth surface, promote cell adherence, immobilization and propagation, maintain surface structure integrity, enable programmable degradation, and thus increase cellular production. The present invention teaches several methods: a method to enhance the integrity of the growth surface by protecting the growth surface in a rigid solid support; a method of use for enhancing the performance of the surface; and a method of modifying a growth surface for eukaryotic and / or prokaryotic cells comprising the steps of increasing surface area by creating porous and 3-D structure, treating a surface to encourage cell attachment, promoting cell growth and proliferation, and disposing the growth surface in any conventional cell cultivating device. The growth surface is able to program degradation and release the cell / tissue mass after the culture is completed.

The invention provides methods for differentiating pluripotent stem cells such as ES cells with improved progenitor and differentiated cell yield using low oxygen conditions and optionally in the absence of exogenously added differentiation factors.

The invention discloses a method for extracting and collecting peripheral blood mononuclear cells. The method comprises the following steps: 1) collecting peripheral blood; 2) pretreating peripheral blood; 3) performing centrifuging; 4) extracting plasma; 5) performing plasma inactivation; 6) collecting mononuclear cell layers; 7) centrifuging the mononuclear cell layers; 8) performing cleaning; and 9) extracting mononuclear cells. The invention belongs to the technical field of cell extraction, and particularly provides a method for separating peripheral blood mononuclear cells, which is usedfor obtaining the peripheral blood mononuclear cells by centrifugal extraction of Ficoll lymphocyte separation liquid and is high in cell yield and high in activity.

The invention discloses a method for isolating peripheral blood mononuclear cells, which comprises the following steps: (1) mixing the peripheral blood with Duchenne's phosphate buffer to obtain diluted peripheral blood; placing the lymphocyte separation solution in a centrifuge tube and Store in shading; (2) Tilt the centrifuge tube containing the lymphocyte separation solution so that the liquid level of the lymphocyte separation solution is at an angle of 50°-70° to the horizontal plane; add the diluted peripheral blood to the lymphocyte along the side wall of the centrifuge tube. The mixed solution is obtained from the cell separation solution; the volume ratio of the diluted peripheral blood to the lymphocyte separation solution is 1-2:1; (3) centrifuge the centrifuge tube containing the mixed solution and take the buffy coat to obtain the peripheral blood mononuclear cell layer. The separation method is easy to operate and is suitable for the separation of a large number of samples; it can realize aseptic operation, and the obtained peripheral blood mononuclear cells are suitable for clinical application; the damage to the isolated peripheral blood mononuclear cells is small, and the cell yield is high. will not be disturbed.

The present invention relates to the field of micro fiuidics. Specifically, the present invention relates to a novel flow cell for the selective enrichment of target particles or cells from a fluid. The flow cell exhibits a novel design which greatly improves the target particle or cell yield. The invention also provides a micro fiuidic device, comprising the flow cell according to the invention.In another aspect, the invention relates to the use of a flow cell or a micro fiuidic device of the invention for the isolation of target particles or cells from a fluid sample. Finally, the inventionrelates to a method for the selective enrichment of target particles or cells from a fluid using the flow cell of the invention.

The invention discloses a method for systematically freezing and storing human umbilical cord tissues according to structure levels. The method comprises the following steps of (1) separation and freeze storage of umbilical cord amniotic membrane tissues; (2) separation and freeze storage of umbilical cord blood vessel tissues; (3) separation and freeze storage of umbilical cord Wharton's jelly tissues. The invention also provides an umbilical cord restoration method. The freeze storage method provided by the invention has the advantages that the umbilical cord amniotic membrane, blood vesselsand Wharton's jelly tissues are sequentially peeled according to the structure levels; then through treatment, the materials are put into liquid nitrogen for freeze storage; three tissues of the umbilical cord are respectively stored; different freeze storage and restoration methods are used according to the specificity of each tissue; the freeze storage restoration activity and cell yield are favorably improved. The invention provides a set of methods for systematically storing complete umbilical cord tissues of various types; important biological resources are provided for the study of thestem cells from the umbilical cord and can be used for building tissue or cell resource sample databases derived from the umbilical cord. The method of the invention provides a solid foundation and basis for completing the human genetic resource databases from the umbilical cord.

A method for promoting biological activity uses a filter system to increase cell production of a fed batch bioreactor. The filter system cycles bioreactor fluid through a hollow fiber tangential flow filter which separates metabolic wastes (as well as proteins) from cells produced in bioreactor and returned to fed batch bioreactor, improving cell production in the fed batch bioreactor. The filter system includes a disposable pump and filter, and a reusable control system. The pump is a low shear gamma stable pump gently cycling bioreactor fluid through the filter with minimal damage to the cells produced in the bioreactor. The pumphead and hollow fiber tangential flow filter are disposable. The pump motor is part of the control system and is reusable. The pumphead and filter are provided as an assembled and pre-sterilized unit allowing simple and quick attachment to the fed batch bioreactor, and simple and quick disposal.