22 results about "Cell yield" patented technology

The invention discloses a tissue dissociation liquid, a tissue dissociation method and application thereof, and relates to the technical field of cell biology. In a DMEM / F12 culture medium, the tissue dissociation liquid only contains the following raw materials in concentration: 0.5 to 3 mg / mL of type II collagenase, 0.5 to 2 mg / mL of type IV collagenase, 0.5 to 2 mg / mL of hyaluronidase, 10 to 18 [mu] M of Y27632 and 80 to 150 [mu] g / mL of primocin. The tissue dissociation liquid provided by the invention can dissociate normal tissues or tumor tissues into single cells or cell clusters, the single cells obtained by the digestion method are high in proliferation rate and easy to successfully culture, and the tissue dissociation liquid has the technical advantages that the cell yield and survival rate are maintained at a relatively high level. Therefore, the tissue dissociation liquid provided by the invention has good application prospects in tumor tissue dissociation, cell line construction and organoid model construction.

The application relates to the technical field of biological sample pretreatment, and discloses a high-efficiency separation method of a tissue cell extraction device. The method comprises the following steps: performing parameter calibration and reference loss modeling of a driving motor, and eliminating background interference of an actuator; applying a disturbance signal to a dissociation chamber, locking the characteristic resonance frequency of a tissue sample through frequency domain energy analysis, and performing pulsating crushing to accelerate the dissociation of a matrix structure; extracting the net torque of the motor in real time, deducing the fluid dynamic viscosity, adaptively adjusting the motor speed according to the viscosity drop trend, maintaining the fluid shear force in a preset target interval, and avoiding mechanical damage to cells; calculating the mechanical energy dissipation power in real time, and performing feedforward temperature control compensation to eliminate temperature overshoot caused by thermal conduction lag. The application realizes closed-loop control of the physical phase evolution of a heterogeneous tissue dissociation process, solves the limitation of a fixed parameter in a traditional method, shortens the dissociation time, and significantly improves the single-cell yield and survival rate.

The process provided in this invention transfects primary cells with gene editing reagents using a high-volume gas-permeable cell culture device and a flow-through electroporation device under conditions that improve gene editing performance, cell yield, and drug (DP) quality characteristics for cell therapy applications. As demonstrated in the examples, primary cells edited according to the process provided herein achieved improved double-strand break (DSB) formation rates, increased frequency of homology-directed repair (HR) and non-homologous end joining (NHEJ) combinations, increased frequency of bi-allelic and mono-allelic HR events, improved cell viability, proliferative capacity, and cell fitness after gene editing, and reduced manufacturing time.

The invention relates to the technical field of cell biology, and particularly discloses an isolated culture method of primary meibomian gland epithelial cells, the obtained primary meibomian gland epithelial cells and application of the primary meibomian gland epithelial cells. The method comprises the following steps: efficiently and mildly digesting meibomian gland tissues by adopting a compound digestive juice consisting of I-type collagenase, dispersing enzyme and trypsin in a specific ratio; stopping digestion by using a serum-containing neutralizing solution and collecting cells; the method comprises the following steps: promoting cell attachment by using a serum-containing initial culture medium, and carrying out selective amplification by using a serum-free proliferation culture medium to finally obtain high-purity and high-activity primary meibomian gland epithelial cells. The method solves the problems of long digestion time, low cell yield, poor purity and the like in the traditional method. The obtained cell can be used for constructing a meibomian gland dysfunction model, screening drugs and researching lipid metabolism, and an important technical platform and a cell model are provided for research and treatment of related eye diseases.

This disclosure relates to the field of solar cell technology, and discloses a back-contact solar cell and a cell module. A first doped structure and a second doped structure are alternately formed on the back surface of a substrate. The first doped structure includes a first busbar and a first extension, and the second doped structure includes a second busbar and a second extension. The width of the first extension is W1, and the width of the second extension is W2, the sum of which is a constant W. An edge doped structure is connected to the busbar closest to the edge of the substrate, and includes an exit region and a connection region. The width of the connection region in a second direction ranges from (a, W), where a = min(W1, W2) or a = W1 = W2. The first extension and the second extension of this disclosure have different widths, and the width of the connection region is greater than the smaller of the two and less than or equal to the larger of the two, balancing carrier transport efficiency and passivation performance, thereby improving cell yield and power generation efficiency.

A solar cell and its fabrication method are disclosed. The fabrication method includes the following steps: depositing a tunneling layer and an amorphous silicon layer on the back side of a P-type silicon substrate, wherein the back side of the silicon substrate has a first region and a second region; fabricating a barrier layer of a first thickness in the electrode region of the first region and a barrier layer of a second thickness in the non-electrode region of the first region; diffusion doping the amorphous silicon layer using an N-type doping source to form an N-type doped crystalline silicon layer and an oxide layer; removing the oxide layer and the N-type doped crystalline silicon layer in the second region; fabricating a first electrode in the electrode region of the first region and a second electrode in the electrode region of the second region. The fabrication method of this invention forms barrier layers of different thicknesses in the first region, which have different blocking effects on phosphorus diffusion. During phosphorus diffusion, a selective emitter is formed in the first region, improving cell efficiency. Furthermore, the barrier layers are corrosion-resistant to alkaline solutions, protecting the N-poly layer in the first region during subsequent processes while completely removing the N-poly layer in the second region, thus improving cell yield.

The application discloses a disposable cell separation device, which comprises a transfer container, a rotary joint and a separation container connected with the rotary joint, the transfer container is communicated with the separation container through a pipeline, the transfer container is provided with a ventilation connecting part for generating positive pressure or negative pressure in the inside of the transfer container, the pipeline is provided with a first docking part for connecting an initial container, a second docking part for connecting a buffer solution container and a third docking part for connecting a collection container. The application also discloses a disposable cell separation device. In the feeding and discharging process, the material does not directly pass through the positive and negative pressure device and is not repeatedly extruded by the positive and negative pressure device, so that cell damage in the feeding and discharging conveying process is avoided and the cell yield and activity are greatly improved. The disposable cell separation device and the cell separation device have the advantages of avoiding cell damage caused by the suction device and greatly improving the cell yield and activity.

The invention discloses a wound cell pre-pressing method and a wound cell pre-pressing device, and relates to the technical field of lithium batteries. Firstly, the staggered arc length of a head tab and a tail tab of a winding battery cell in the pre-pressing direction is obtained, the winding battery cell is in a circular ring shape, the head tab is located on the inner side of the ring of the winding battery cell, and the tail tab is located on the outer side of the ring of the winding battery cell; then obtaining the ring width of the winding battery cell; calculating a dislocation angle between the head tab and the tail tab according to the dislocation arc length and the ring width; then controlling the winding battery cell to rotate around the axis of the winding battery cell by a dislocation angle, so that the head tab and the tail tab are aligned in the pre-pressing direction; and pre-pressing the wound battery cell along the pre-pressing direction. According to the pre-pressing method for the wound battery cell, provided by the invention, accurate adjustment of the pre-pressing point position can be realized, tab dislocation is improved, the performance of the battery cell is ensured, the yield of the battery cell is improved, non-uniform current distribution is avoided, and the safety is improved.

The invention discloses a method for separating and extracting PBMC (peripheral blood mononuclear cells), which comprises the following steps: on the premise of retaining PBMC in a sample, carrying out volume reduction treatment on a sample solution, namely, carrying out volume reduction on the sample solution to discharge a certain amount of plasma and red blood cells, and then carrying out ficoll solution centrifugation, so as to carry out one-time PBMC separation operation on a large-volume sample; the process flow time of PBMC separation and extraction is greatly shortened, the cell yield is high, the activity is high, and an effective cell acquisition way is provided for clinical and basic cytology research so as to facilitate subsequent immune cell experiments; according to the separation and extraction method, less separation liquid, washing liquid (replacement liquid) and disposable consumables are used, so that the time, consumables and reagent cost are saved.

The invention discloses an amplification culture medium, an amplification method and application of an iPSC (induced pluripotent stem cell) derived hematopoietic progenitor cell population, and relates to the technical field of cell culture. According to the amplification culture medium provided by the invention, animal-derived components are replaced by chemical small molecule combinations, so that the safety of cell products is remarkably improved; the yield of NK cells cultured by the culture medium is greatly increased, the HPC amplification multiple is increased to 2.5 times or more of that of a traditional process by adding tranexamic acid, and the bottleneck of industrial production is broken through. The method provided by the invention improves the process stability. The cells amplified according to the method provided by the invention strengthen the pertinence of lung cancer treatment.

The invention relates to a solar cell and a preparation method thereof. The preparation method comprises the following steps that a battery piece to be plated is provided, the battery piece to be plated comprises a battery piece body, a seed layer and a grid line, the seed layer is arranged on the battery piece body, and the grid line is arranged on the seed layer; and applying an electric field to the surface of the battery piece to be plated, and plating a protective film layer on the grid line through a chemical plating process under the action of the electric field. An electric field is applied to the surface of a to-be-plated battery piece in the process of plating a protective film layer through the chemical plating process, electrostatic shielding is generated on the surface of the to-be-plated battery piece, a closed Faraday cage is formed, no external electron flows in the chemical plating process, and the surface of the to-be-plated battery piece is protected. The replacement reaction of chemical plating in the non-grid line conductive area on the surface of the battery piece is reduced, the damage to the seed layer is reduced, the grid line binding force is improved, and the battery yield is improved.

The invention relates to a culture medium for culturing human mycoplasma pneumoniae and a culture method, and belongs to the technical field of microorganisms, the culture medium for culturing the human mycoplasma pneumoniae comprises a mycoplasma culture medium and a growth promoting additive, the growth promoting additive comprises horse serum or fetal calf serum, cholesterol, penicillin sodium and an HEPES buffer solution, according to the culture medium for culturing the human mycoplasma pneumoniae, various components are simple and easy to obtain, the preparation method of the culture medium is greatly simplified, and the efficiency is improved; according to the culture method of the human mycoplasma pneumoniae, the culture mode is changed from a static state to a dynamic state, so that the culture efficiency of the human mycoplasma pneumoniae is improved, and meanwhile, the thallus yield of the human mycoplasma pneumoniae is also improved; the culture method for preparing the human mycoplasma pneumoniae is short in period, low in cost, high in culture viable organism density and high in yield, and is suitable for large-scale culture of the human mycoplasma pneumoniae.

The invention discloses an N-type TOPCon battery PSG removing machine capable of automatically adjusting the difference value of oxide layers, and belongs to the technical field of TOPCon battery production. The N-type TOPCon battery PSG removing machine comprises a PSG removing machine body (1A); a feeding device and a discharging device are arranged on the two sides of the PSG removing machine body (A) respectively; a first oxide layer testing device and a second oxide layer testing device are arranged at the discharging end of the feeding device and the feeding end of the discharging device respectively, the PSG removing machine body (A) comprises a machine shell placed on the ground, and one side of an inner cavity of the machine shell communicates with an HF mixed liquid injection channel. According to the method, the oxide layer testing devices are added before and after PSG removal, and according to the difference value of the front oxide layer and the rear oxide layer, process adjustment and equipment maintenance improvement can be carried out on the machine deviating from the central value in time, so that the phenomenon of over-washing or under-washing is effectively reduced, the EL dark piece and electric leakage of the finished battery piece are improved, and the performances such as the yield and the efficiency of the battery piece are improved.

This invention discloses a chicken embryo fibroblast, its preparation method, and its application, relating to the field of biopharmaceutical technology. The preparation method of the chicken embryo fibroblast includes the following steps: S1, providing chicken embryos and obtaining the muscle tissue of the chicken embryos; S2, mixing the muscle tissue with a plant-derived compound digestion solution, and sequentially performing digestion, purification, and primary culture treatment using a serum-free special culture medium to obtain primary chicken embryo fibroblasts; wherein, the plant-derived compound digestion solution includes bromelain and papain. This invention, using a plant-derived compound digestion solution and a serum-free special culture medium, significantly improves the batch-to-batch stability, cell yield, and cell purity of the cells, reducing the risk of exogenous viral contamination. Therefore, the prepared chicken embryo fibroblasts exhibit high batch-to-batch stability and good reproducibility in viral culture.

The application discloses a cell non-damage delivery system, which comprises an initial container, a processing device, a collecting container, a transfer container and a suction device for generating positive and negative pressure in the transfer container. The transfer container is communicated with the initial container through a first connecting pipe, communicated with the processing device through a second connecting pipe and communicated with the collecting container through a third connecting pipe. A first valve body is arranged on the first connecting pipe, a second valve body is arranged on the second connecting pipe and a third valve body is arranged on the third connecting pipe. Cells in the initial container are delivered to the processing device through the transfer container, treated in the processing device and then delivered to the collecting container through the transfer container. The cell non-damage delivery system has the advantages of avoiding cell damage caused by the suction device and greatly improving cell yield and activity.

Methods and systems are disclosed for manipulating inert materials and biomaterials, including cell cultures, to efficiently form effective cell beds while preventing excess flow through of cells to permeate waste during bioprocessing. Gentle centrifugation concentrates a large volume of cells produced from bioreactors into the desired concentrated volume and cell density. When cells pass through the centrifuge, the majority fraction of cells are retained in the centrifuge disposable chamber pods as a cell bed. A recirculation loop redirects the remaining minority fraction of cells back to the cell bag instead of proceeding to waste. This prevents initial cell loss during cell bed formation in the chamber pods, increases overall cell yields at harvest, and conserves materials, for example. Growing and harvesting natural killer cells, in particular, increased yields by over 30% when the recirculation loop was employed.

The invention provides a preparation method of mesenchymal stem cell-like precursor cells, a culture medium composition and application of the culture medium composition, and relates to the technical field of biology. The preparation method comprises the following steps: using a second-stage culture medium to differentiate pre-induced induced pluripotent stem cells for 12-36 hours, then replacing the second-stage culture medium with a third-stage culture medium to continue induced differentiation for 70-74 hours, and then using a fourth-stage culture medium to perform cell self-adaptive culture for 44-50 hours, the second-stage culture medium and the third-stage culture medium both contain a pluripotent stem cell culture medium and a GSK-3beta pathway inhibitor, and the concentration of the GSK-3beta pathway inhibitor in the third-stage culture medium is higher than that of the second-stage culture medium; the fourth-stage culture medium contains a mesenchymal stem cell serum-free culture medium and 0-2ng / mL TGF beta-1. The preparation method is stable in quantity of harvested cells and high in differentiation efficiency; the obtained mesenchymal stem cell-like precursor cells are high in proliferation activity, clear in cell phenotype and high in uniformity.

The invention discloses a method for in-situ online monitoring of a cell state in a fermentation process, an automatic sampling system and application of the automatic sampling system. Collecting fermentation broth at different fermentation time points from the fermentation tank by using an automatic sampling system, and introducing the fermentation broth into the micro-fluidic chip; collecting the Raman spectrum of the single cell in the micro-fluidic chip by using a Raman spectrum system; preprocessing the obtained single-cell Raman spectrum data, performing feature extraction and dimension reduction through an unsupervised self-encoding algorithm, performing clustering analysis through an unsupervised clustering algorithm, dividing cell states based on a clustering analysis result, and constructing a single-cell state model; and establishing a relationship between the average Raman spectrum of the sampling points and the product content of unit cells by using a multivariable statistical regression analysis algorithm, and constructing a yield prediction model. According to the invention, the state of the microbial single cell in the fermentation tank can be monitored in real time, on line and with high throughput, and the cell yield can be directly predicted to realize the in-situ on-line monitoring of the single cell in the fermentation process.

The invention provides a preparation method and application of a multi-type CAR-immune cell mixed product containing CAR-macrophages and T cells, and relates to the technical field of biology. The preparation method of the mixed product containing the CAR-macrophages and the T cells, provided by the invention, comprises the following steps: culturing peripheral blood mononuclear cells obtained by separation in a culture medium, introducing a gene for coding a chimeric antigen receptor into the macrophages, and continuously culturing to obtain the multi-type CAR-immune cell mixed product containing the CAR-macrophages and the T cells. According to the preparation method, the mixed multi-type CAR-immune cell (CAR-mix) containing CAR-macrophages and T cells is prepared by adopting a one-step method, the cell yield is high, the proportion of the CAR-macrophages to the T cells in the prepared immune cell is proper, the synergistic effect of the CAR-macrophages and the T cells can be fully exerted, the generation of polyclonal T cells is stimulated, and the CAR-mix is used for preparing the CAR-macrophages and the T cells. A stronger solid tumor resisting function is realized.

The invention relates to a method for obtaining crystalline fetal calf serum by adding agmatine with a specific concentration. The method plays a role in increasing the cell yield and increasing the protein expression quantity per cell. The method also involves reconstitution into a specific form of liquid for use of the liquid without losing its original properties, allowing storage without ultra-low temperature chains and reducing the risk of contamination, thereby allowing precise dosing of the amount to be used.

The invention belongs to the technical field of solar cells, and particularly discloses an additive for removing a TOPCon cell winding plating mark and a use method of the additive. The invention relates to an additive for removing a TOPCon battery wound plating mark, which comprises the following components in percentage by mass: 4 to 6 percent of polishing agent, 0.02 to 0.04 percent of protective agent, 0.2 to 0.4 percent of complexing agent, 2 to 4 percent of defoaming agent, 0.1 to 0.2 percent of hydroxamic acid compound, 0.1 to 1 percent of piperazinyl silane compound, 0.5 to 1.5 percent of sugar alcohol and the balance of deionized water, the additive disclosed by the invention not only can completely remove the winding plating mark without winding plating mark residue, but also can protect the BSG layer from being corroded, so that the yield and the efficiency of the battery are ensured.

This invention discloses an explosion-proof silicon solar cell based on physical deposition technology and its manufacturing process. The cell structure includes a silicon substrate, with a P++ emitter, an aluminum oxide layer, a silicon nitride layer, and a metal electrode sequentially formed on the front side from the inside out. The back side is formed with a silicon oxide tunneling layer, a lightly doped and low-hydrogen polycrystalline silicon layer, a heavily doped and hydrogen-rich polycrystalline silicon layer, a silicon nitride layer, and a metal electrode sequentially from the inside out. The explosion-proof silicon solar cell manufacturing process of this invention is based on PVD technology, which does not cause winding plating. By adjusting the [H] content in the TOPCon structure, the occurrence of explosion defects is reduced, and subsequent ammonia annealing replenishes the [H] content, ensuring the passivation capability of the film. Compared with other disclosed preparation technologies, this PVD-based explosion-proof film process reduces the occurrence of explosion phenomena without winding plating, improves cell yield, and reduces defective products in module manufacturing.