43 results about "Cell yield" patented technology

The invention discloses a gelatin microcarrier lysate as well as a preparation method and application thereof. The gelatin microcarrier lysis buffer comprises lyase, a cell protective agent, a cell energy metabolism protective agent, an antioxidant, a buffer solution and a chelating agent. The gelatin microcarrier lysate has extremely strong protectiveness to cells, abandons damaging trypsin and EDTA, and innovatively adds a cell protection combination of a cell protective agent L-arginine, a cell energy metabolism protective agent sodium pyruvate and an antioxidant vitamin C, so that the cell survival rate (gt; 98%) and the retention rate (gt; the method is high in cell yield, good in safety and wide in range.

The invention discloses a tissue dissociation liquid, a tissue dissociation method and application thereof, and relates to the technical field of cell biology. In a DMEM / F12 culture medium, the tissue dissociation liquid only contains the following raw materials in concentration: 0.5 to 3 mg / mL of type II collagenase, 0.5 to 2 mg / mL of type IV collagenase, 0.5 to 2 mg / mL of hyaluronidase, 10 to 18 [mu] M of Y27632 and 80 to 150 [mu] g / mL of primocin. The tissue dissociation liquid provided by the invention can dissociate normal tissues or tumor tissues into single cells or cell clusters, the single cells obtained by the digestion method are high in proliferation rate and easy to successfully culture, and the tissue dissociation liquid has the technical advantages that the cell yield and survival rate are maintained at a relatively high level. Therefore, the tissue dissociation liquid provided by the invention has good application prospects in tumor tissue dissociation, cell line construction and organoid model construction.

The present invention belongs to the technical field of solar cells, specifically to a method for selective low-temperature annealing of solar cell electrodes. The method comprises performing periodic rapid temperature-increasing pulse annealing on a metal electrode and its contact interface with a semiconductor under nitrogen protection, wherein the duty ratio of the electrically controlled pulses within each cycle is 1:5, the annealing temperature within each cycle does not exceed 200°C, the heating time within each cycle does not exceed 1 minute, and the heating rate within each cycle does not exceed 5°C / s. Subsequently, the contact resistance between the metal electrode and the semiconductor is measured. The present invention effectively solves the problem of ohmic contact between the metal electrodes of a solar cell and with the semiconductor material, while controlling the depth and range of heat application, avoiding damage to high-temperature-sensitive materials such as the organic material bonding layer in the cell. This method improves cell performance while addressing cell yield issues. The method is applicable to most devices requiring low-temperature annealing.

The application relates to the technical field of biological sample pretreatment, and discloses a high-efficiency separation method of a tissue cell extraction device. The method comprises the following steps: performing parameter calibration and reference loss modeling of a driving motor, and eliminating background interference of an actuator; applying a disturbance signal to a dissociation chamber, locking the characteristic resonance frequency of a tissue sample through frequency domain energy analysis, and performing pulsating crushing to accelerate the dissociation of a matrix structure; extracting the net torque of the motor in real time, deducing the fluid dynamic viscosity, adaptively adjusting the motor speed according to the viscosity drop trend, maintaining the fluid shear force in a preset target interval, and avoiding mechanical damage to cells; calculating the mechanical energy dissipation power in real time, and performing feedforward temperature control compensation to eliminate temperature overshoot caused by thermal conduction lag. The application realizes closed-loop control of the physical phase evolution of a heterogeneous tissue dissociation process, solves the limitation of a fixed parameter in a traditional method, shortens the dissociation time, and significantly improves the single-cell yield and survival rate.

The invention provides a culture method and preparation of bifidobacterium longum subsp.infantis B8762 based on carbon source regulation and control, and belongs to the technical field of biology. The method comprises the following steps: inoculating an activated strain into a carbon-source-free improved MRS culture medium, adding a specific carbon source, monitoring a growth curve in real time through an on-line living cell sensor, and analyzing and regulating the growth stage and activity of thalli in combination with metabolites. By precisely regulating and controlling a substrate, the cell activity is remarkably improved, the proportion of living cells is increased, the sugar uptake ability and the key metabolic enzyme activity are enhanced, a growth curve is optimized, and the cell yield coefficient and the substrate conversion efficiency are improved. A bacteriostatic preparation, a viable bacterium preparation and a metabolite preparation can be prepared and respectively have specific metabolite concentration, living cell proportion and enzyme activity. The culture method and preparation of the bifidobacterium longum subsp.infantis B8762 based on carbon source regulation and control are simple to operate and easy to industrialize, the production cost can be reduced, the production efficiency can be improved, and the requirements of different application scenes can be met.

The process provided in this invention transfects primary cells with gene editing reagents using a high-volume gas-permeable cell culture device and a flow-through electroporation device under conditions that improve gene editing performance, cell yield, and drug (DP) quality characteristics for cell therapy applications. As demonstrated in the examples, primary cells edited according to the process provided herein achieved improved double-strand break (DSB) formation rates, increased frequency of homology-directed repair (HR) and non-homologous end joining (NHEJ) combinations, increased frequency of bi-allelic and mono-allelic HR events, improved cell viability, proliferative capacity, and cell fitness after gene editing, and reduced manufacturing time.

The invention relates to the technical field of cell biology, and particularly discloses an isolated culture method of primary meibomian gland epithelial cells, the obtained primary meibomian gland epithelial cells and application of the primary meibomian gland epithelial cells. The method comprises the following steps: efficiently and mildly digesting meibomian gland tissues by adopting a compound digestive juice consisting of I-type collagenase, dispersing enzyme and trypsin in a specific ratio; stopping digestion by using a serum-containing neutralizing solution and collecting cells; the method comprises the following steps: promoting cell attachment by using a serum-containing initial culture medium, and carrying out selective amplification by using a serum-free proliferation culture medium to finally obtain high-purity and high-activity primary meibomian gland epithelial cells. The method solves the problems of long digestion time, low cell yield, poor purity and the like in the traditional method. The obtained cell can be used for constructing a meibomian gland dysfunction model, screening drugs and researching lipid metabolism, and an important technical platform and a cell model are provided for research and treatment of related eye diseases.

To provide a differentiation inhibitor for stem cell culture which can inhibit differentiation of stem cells and increase cell yield when the stem cells are subjected to stirred culture.SOLUTION: A differentiation inhibitor for stem cell culture comprising, as an active ingredient, an alkaline extract protein of tea leaves. It is preferable that the tea leaves are residues obtained after hot-water extraction treatment of tea leaves. It is also preferable that the alkaline extract protein has a molecular weight of 3000 or more. Furthermore, it is preferable that the tea leaves are green tea.SELECTED DRAWING: None

This disclosure relates to the field of solar cell technology, and discloses a back-contact solar cell and a cell module. A first doped structure and a second doped structure are alternately formed on the back surface of a substrate. The first doped structure includes a first busbar and a first extension, and the second doped structure includes a second busbar and a second extension. The width of the first extension is W1, and the width of the second extension is W2, the sum of which is a constant W. An edge doped structure is connected to the busbar closest to the edge of the substrate, and includes an exit region and a connection region. The width of the connection region in a second direction ranges from (a, W), where a = min(W1, W2) or a = W1 = W2. The first extension and the second extension of this disclosure have different widths, and the width of the connection region is greater than the smaller of the two and less than or equal to the larger of the two, balancing carrier transport efficiency and passivation performance, thereby improving cell yield and power generation efficiency.

The invention belongs to the technical field of immune cell detection, and particularly relates to a detection reagent for immune cells and a preparation and detection method of the detection reagent. S2, carrying out enzymolysis digestion; s3, preparing a single-cell suspension; s4, sorting the immunomagnetic beads; s5, carrying out flow dyeing; s6, assembling the kit; according to the immune cell detection reagent and the preparation and detection method, through the synergistic effect of different enzymes, tissues can be decomposed mildly and effectively, the excessive digestion situation possibly caused by only using a certain enzyme is prevented, damage to cell activity is reduced, the activity requirement of cells in subsequent detection is guaranteed, and then the accuracy of a detection result is guaranteed; secondly, various enzymes are used for decomposing different components in the tissue, so that the tissue can be digested into a single-cell suspension more quickly and thoroughly compared with a single enzyme, the cell yield is increased, and enough cells are provided for the subsequent steps of immunomagnetic bead sorting and the like.

The invention belongs to the technical field of biomedicine, and particularly relates to a method for preparing a cell suspension through animal tendon dissociation. By using the method disclosed by the invention, the dissociated cell suspension can meet the requirements of single cell sequencing in the aspects of activity, living cell quantity, caking rate, cell yield and the like, and the method disclosed by the invention is simple and convenient in preparation flow and lower in cost.

A solar cell and its fabrication method are disclosed. The fabrication method includes the following steps: depositing a tunneling layer and an amorphous silicon layer on the back side of a P-type silicon substrate, wherein the back side of the silicon substrate has a first region and a second region; fabricating a barrier layer of a first thickness in the electrode region of the first region and a barrier layer of a second thickness in the non-electrode region of the first region; diffusion doping the amorphous silicon layer using an N-type doping source to form an N-type doped crystalline silicon layer and an oxide layer; removing the oxide layer and the N-type doped crystalline silicon layer in the second region; fabricating a first electrode in the electrode region of the first region and a second electrode in the electrode region of the second region. The fabrication method of this invention forms barrier layers of different thicknesses in the first region, which have different blocking effects on phosphorus diffusion. During phosphorus diffusion, a selective emitter is formed in the first region, improving cell efficiency. Furthermore, the barrier layers are corrosion-resistant to alkaline solutions, protecting the N-poly layer in the first region during subsequent processes while completely removing the N-poly layer in the second region, thus improving cell yield.

The application discloses a disposable cell separation device, which comprises a transfer container, a rotary joint and a separation container connected with the rotary joint, the transfer container is communicated with the separation container through a pipeline, the transfer container is provided with a ventilation connecting part for generating positive pressure or negative pressure in the inside of the transfer container, the pipeline is provided with a first docking part for connecting an initial container, a second docking part for connecting a buffer solution container and a third docking part for connecting a collection container. The application also discloses a disposable cell separation device. In the feeding and discharging process, the material does not directly pass through the positive and negative pressure device and is not repeatedly extruded by the positive and negative pressure device, so that cell damage in the feeding and discharging conveying process is avoided and the cell yield and activity are greatly improved. The disposable cell separation device and the cell separation device have the advantages of avoiding cell damage caused by the suction device and greatly improving the cell yield and activity.

The application discloses a kind of northern Shaanxi white cashmere goat muscle satellite cell separation and purification method, the present application uses northern Shaanxi white cashmere goat fetus dorsal longest muscle tissue as test material, by aseptic pretreatment, tissue block inversion adherent culture, primary subculture culture Combination of compound technology of hierarchical differential adherent purification, stably obtain the 5th generation muscle satellite cell.The present application optimizes and adapts culture system and purification process, overcomes the defects of low cell yield, serious interference of mixed cells, activity and poor purity of traditional preparation method.At the same time, the present application also adopts RT-PCR molecular identification and immunofluorescence staining combined with multi-level verification method, from gene and protein level Bidirectional verification cell specificity and differentiation potential, accurately determine the biological properties of cell.The present application process is simple, good stability and repeatability, can efficiently prepare northern Shaanxi white cashmere goat muscle satellite cell, provides important technical support for cashmere goat germplasm resource conservation, muscle development mechanism and muscle regeneration related research.

The invention discloses a wound cell pre-pressing method and a wound cell pre-pressing device, and relates to the technical field of lithium batteries. Firstly, the staggered arc length of a head tab and a tail tab of a winding battery cell in the pre-pressing direction is obtained, the winding battery cell is in a circular ring shape, the head tab is located on the inner side of the ring of the winding battery cell, and the tail tab is located on the outer side of the ring of the winding battery cell; then obtaining the ring width of the winding battery cell; calculating a dislocation angle between the head tab and the tail tab according to the dislocation arc length and the ring width; then controlling the winding battery cell to rotate around the axis of the winding battery cell by a dislocation angle, so that the head tab and the tail tab are aligned in the pre-pressing direction; and pre-pressing the wound battery cell along the pre-pressing direction. According to the pre-pressing method for the wound battery cell, provided by the invention, accurate adjustment of the pre-pressing point position can be realized, tab dislocation is improved, the performance of the battery cell is ensured, the yield of the battery cell is improved, non-uniform current distribution is avoided, and the safety is improved.

Methods and systems are disclosed for manipulating inert materials and biomaterials, including cell cultures, to efficiently form effective cell beds while preventing excess flow through of cells to permeate waste during bioprocessing. Gentle centrifugation concentrates a large volume of cells produced from bioreactors into the desired concentrated volume and cell density. When cells pass through the centrifuge, the majority fraction of cells are retained in the centrifuge disposable chamber pods as a cell bed. A recirculation loop redirects the remaining minority fraction of cells back to the cell bag instead of proceeding to waste. This prevents initial cell loss during cell bed formation in the chamber pods, increases overall cell yields at harvest, and conserves materials, for example. Growing and harvesting natural killer cells, in particular, increased yields by over 30% when the recirculation loop was employed.

The invention discloses a method for separating and extracting PBMC (peripheral blood mononuclear cells), which comprises the following steps: on the premise of retaining PBMC in a sample, carrying out volume reduction treatment on a sample solution, namely, carrying out volume reduction on the sample solution to discharge a certain amount of plasma and red blood cells, and then carrying out ficoll solution centrifugation, so as to carry out one-time PBMC separation operation on a large-volume sample; the process flow time of PBMC separation and extraction is greatly shortened, the cell yield is high, the activity is high, and an effective cell acquisition way is provided for clinical and basic cytology research so as to facilitate subsequent immune cell experiments; according to the separation and extraction method, less separation liquid, washing liquid (replacement liquid) and disposable consumables are used, so that the time, consumables and reagent cost are saved.

The invention discloses a method for separating colonic lamina propria cells of mice with colitis and application of the method. According to the method, separation of the propria neutrophile granulocyte on the colon tissue with the complete length is tried for the first time, and the total time required by cell separation is effectively shortened through multiple digestion reagents with different compositions, so that apoptosis of the neutrophile granulocyte caused by long-time exposure of an intestinal sample is effectively avoided; the problems of low cell yield, poor activity and the like are solved, and a new thought is provided for separation of the neutrophile granulocytes.

The invention belongs to the technical field of molecular biology, and particularly relates to a tartary buckwheat protoplast separation and transient expression system construction method. The technical problem to be solved by the invention is that a tartary buckwheat protoplast separation technology is immature. According to the technical scheme, the method for separating the tartary buckwheat protoplast comprises the following steps: taking a tartary buckwheat hypocotyl tissue to an enzymatic hydrolysate for enzymolysis; and terminating the enzymolysis reaction, cleaning, and collecting the precipitate to obtain the protoplast. The invention also provides a tartary buckwheat protoplast instantaneous conversion system. According to the construction method, the tartary buckwheat hypocotyl cultured in darkness is used, the source is easy to obtain, chloroplast does not exist, observation is not affected, and after a vacuum pump is used for suction, the cell yield is greatly increased, and the cell activity is not affected. On the basis of protoplast separation, the invention further establishes a protoplast instantaneous conversion technology which can be applied to tartary buckwheat.

This invention discloses a centrifugal recovery device, relating to the field of biological cell processing technology. The centrifugal recovery device includes a centrifuge container and a paddle assembly. The centrifuge container includes a centrifuge chamber, with a collection area at the top for easy discharge of waste liquid. The paddle assembly is located within the centrifuge chamber and includes paddles and a paddle shaft. The centrifuge container and the paddle shaft share the same axis of rotation. A first flow channel and a second flow channel are formed between the paddles and the centrifuge container. The paddle shaft has an axially arranged inlet channel and a recovery channel. The inlet channel communicates with the centrifuge chamber. The first flow channel is arranged on the inner circumference of the centrifuge container, with one side communicating with the inner cavity of the centrifuge chamber. The second flow channel communicates with both the first flow channel and the recovery channel. This invention provides a continuous flow centrifugal recovery device for cell processing, which features a long cell fluid movement path, improving centrifugation efficiency and effectiveness, resulting in more thorough separation of cells from the supernatant, and increasing cell yield and viability.

The invention discloses an amplification culture medium, an amplification method and application of an iPSC (induced pluripotent stem cell) derived hematopoietic progenitor cell population, and relates to the technical field of cell culture. According to the amplification culture medium provided by the invention, animal-derived components are replaced by chemical small molecule combinations, so that the safety of cell products is remarkably improved; the yield of NK cells cultured by the culture medium is greatly increased, the HPC amplification multiple is increased to 2.5 times or more of that of a traditional process by adding tranexamic acid, and the bottleneck of industrial production is broken through. The method provided by the invention improves the process stability. The cells amplified according to the method provided by the invention strengthen the pertinence of lung cancer treatment.

The invention relates to a solar cell and a preparation method thereof. The preparation method comprises the following steps that a battery piece to be plated is provided, the battery piece to be plated comprises a battery piece body, a seed layer and a grid line, the seed layer is arranged on the battery piece body, and the grid line is arranged on the seed layer; and applying an electric field to the surface of the battery piece to be plated, and plating a protective film layer on the grid line through a chemical plating process under the action of the electric field. An electric field is applied to the surface of a to-be-plated battery piece in the process of plating a protective film layer through the chemical plating process, electrostatic shielding is generated on the surface of the to-be-plated battery piece, a closed Faraday cage is formed, no external electron flows in the chemical plating process, and the surface of the to-be-plated battery piece is protected. The replacement reaction of chemical plating in the non-grid line conductive area on the surface of the battery piece is reduced, the damage to the seed layer is reduced, the grid line binding force is improved, and the battery yield is improved.

The invention relates to a culture medium for culturing human mycoplasma pneumoniae and a culture method, and belongs to the technical field of microorganisms, the culture medium for culturing the human mycoplasma pneumoniae comprises a mycoplasma culture medium and a growth promoting additive, the growth promoting additive comprises horse serum or fetal calf serum, cholesterol, penicillin sodium and an HEPES buffer solution, according to the culture medium for culturing the human mycoplasma pneumoniae, various components are simple and easy to obtain, the preparation method of the culture medium is greatly simplified, and the efficiency is improved; according to the culture method of the human mycoplasma pneumoniae, the culture mode is changed from a static state to a dynamic state, so that the culture efficiency of the human mycoplasma pneumoniae is improved, and meanwhile, the thallus yield of the human mycoplasma pneumoniae is also improved; the culture method for preparing the human mycoplasma pneumoniae is short in period, low in cost, high in culture viable organism density and high in yield, and is suitable for large-scale culture of the human mycoplasma pneumoniae.

The invention discloses an N-type TOPCon battery PSG removing machine capable of automatically adjusting the difference value of oxide layers, and belongs to the technical field of TOPCon battery production. The N-type TOPCon battery PSG removing machine comprises a PSG removing machine body (1A); a feeding device and a discharging device are arranged on the two sides of the PSG removing machine body (A) respectively; a first oxide layer testing device and a second oxide layer testing device are arranged at the discharging end of the feeding device and the feeding end of the discharging device respectively, the PSG removing machine body (A) comprises a machine shell placed on the ground, and one side of an inner cavity of the machine shell communicates with an HF mixed liquid injection channel. According to the method, the oxide layer testing devices are added before and after PSG removal, and according to the difference value of the front oxide layer and the rear oxide layer, process adjustment and equipment maintenance improvement can be carried out on the machine deviating from the central value in time, so that the phenomenon of over-washing or under-washing is effectively reduced, the EL dark piece and electric leakage of the finished battery piece are improved, and the performances such as the yield and the efficiency of the battery piece are improved.

This invention discloses a chicken embryo fibroblast, its preparation method, and its application, relating to the field of biopharmaceutical technology. The preparation method of the chicken embryo fibroblast includes the following steps: S1, providing chicken embryos and obtaining the muscle tissue of the chicken embryos; S2, mixing the muscle tissue with a plant-derived compound digestion solution, and sequentially performing digestion, purification, and primary culture treatment using a serum-free special culture medium to obtain primary chicken embryo fibroblasts; wherein, the plant-derived compound digestion solution includes bromelain and papain. This invention, using a plant-derived compound digestion solution and a serum-free special culture medium, significantly improves the batch-to-batch stability, cell yield, and cell purity of the cells, reducing the risk of exogenous viral contamination. Therefore, the prepared chicken embryo fibroblasts exhibit high batch-to-batch stability and good reproducibility in viral culture.

To provide: processes for differentiating an endodermal cell into a posterior foregut cell, a posterior foregut cell into a hepatic progenitor cell, and / or a hepatic progenitor cell into a hepatocyte-like cell; and obtained cell populations.SOLUTION: In some embodiments, the process can be conducted in the absence of serum. The hepatocyte-like cell population obtained from this process have a detectable CyP3A4 activity and / or express a detectable level of albumin and / or of urea. The process can be designed to increase cellular yield.SELECTED DRAWING: None

The application discloses a cell non-damage delivery system, which comprises an initial container, a processing device, a collecting container, a transfer container and a suction device for generating positive and negative pressure in the transfer container. The transfer container is communicated with the initial container through a first connecting pipe, communicated with the processing device through a second connecting pipe and communicated with the collecting container through a third connecting pipe. A first valve body is arranged on the first connecting pipe, a second valve body is arranged on the second connecting pipe and a third valve body is arranged on the third connecting pipe. Cells in the initial container are delivered to the processing device through the transfer container, treated in the processing device and then delivered to the collecting container through the transfer container. The cell non-damage delivery system has the advantages of avoiding cell damage caused by the suction device and greatly improving cell yield and activity.

Methods and systems are disclosed for manipulating inert materials and biomaterials, including cell cultures, to efficiently form effective cell beds while preventing excess flow through of cells to permeate waste during bioprocessing. Gentle centrifugation concentrates a large volume of cells produced from bioreactors into the desired concentrated volume and cell density. When cells pass through the centrifuge, the majority fraction of cells are retained in the centrifuge disposable chamber pods as a cell bed. A recirculation loop redirects the remaining minority fraction of cells back to the cell bag instead of proceeding to waste. This prevents initial cell loss during cell bed formation in the chamber pods, increases overall cell yields at harvest, and conserves materials, for example. Growing and harvesting natural killer cells, in particular, increased yields by over 30% when the recirculation loop was employed.

The invention provides a method for separating mononuclear cells of umbilical cord blood, and relates to the technical field of mononuclear cell separation. The separation method of the umbilical cord blood mononuclear cells comprises the following steps: centrifugally separating upper plasma from umbilical cord blood added with an anticoagulant, mixing the upper plasma with a diluent, carrying out oxygenation and pressurization treatment, mixing with a pre-cooled separating medium, standing to recover to normal temperature, carrying out centrifugal layering, collecting the mixed diluent of a mononuclear cell layer, continuously centrifuging, and reserving a precipitate to obtain the umbilical cord blood mononuclear cells. The defects in the prior art are overcome, the separation efficiency of the umbilical cord blood mononuclear cells is effectively improved, and the final mononuclear cell yield is improved under the condition that the cell activity is not affected.

Provided is a high-capacity cell pack in which cell imbalance is eliminated, precipitation of a poorly soluble redox shuttle can be prevented, deterioration due to overcharging is suppressed, cell yield is improved, and charge / discharge cycling is stable. The cell pack according to the present invention is a cell pack comprising non-aqueous secondary cells each comprising a non-aqueous electrolyte solution containing an electrolyte salt and a non-aqueous solvent, a positive electrode, and a negative electrode, wherein the cell pack is configured with a module in which two or more of the non-aqueous secondary cells are connected in series or with two or more of the modules in parallel, or the cell pack is configured with a module in which two or more of the non-aqueous secondary cells are connected in parallel or with two or more of the modules in series.