278 results about "Cell isolation" patented technology

The present application describes various applications of the non-expansion protocol for the preparation of an injectable autologous cell mixture of the present invention that can be used to prevent symptoms in a number of indications. Cells are isolated from surgically derived tissue and are at least partially disaggregated from each other. The heterologous cell mixture is mixed with growth factors, differentiation agents, extracellular matrix proteins and/or microspheres and injected into the patient without cell expansion. The harvesting of tissue, cell isolation, and injection are performed within a single surgical procedure lasting only minutes to hours.

This invention provides: agents determined to be capable of specifically inhibiting the fusion of a macrophage-tropic primary isolate of HIV-1 to a CD4⁺ cell, but not a T cell-tropic isolate of HIV-1 to a CD4⁺ cell; and agents determined to be capable of specifically inhibiting the fusion of a T cell-tropic isolate of HIV-1 to a CD4⁺ cell, but not a macrophage-tropic primary isolate of HIV-1 to a CD4⁺ cell. This invention also provides: agents capable of specifically inhibiting the fusion of a macrophage tropic primary isolate of HIV-1 with a CD+ cell susceptible to infection by a macrophage-tropic primary isolate of HIV-1; and agents capable of specifically inhibiting the fusion of a T cell-tropic isolate of HIV-1 with a CD4⁺ cell susceptible to infection by a T cell-tropic isolate of HIV-1. The agents include but are not limited to antibodies. This invention further provides: methods of inhibiting fusion of a macrophage-tropic primary isolate of HIV-1 with a CD+ cell susceptible to infection by a macrophage-tropic primary isolate of HIV-1 which comprises contacting the CD4⁺ cell with an amount of an agent capable of specifically inhibiting such fusion so as to thereby inhibit such fusion; and methods of inhibiting fusion of a T cell-tropic isolate of HIV-1 with a CD4⁺ cell susceptible to infection by a T cell-tropic isolate of HIV-1 which comprises contacting the CD4⁺ cell with an amount of an agent capable of specifically inhibiting such fusion so as to thereby inhibit such fusion.

A one-step real-time system for treating, reconstructing or augmenting a breast tissue defect in a patient comprising means for extracting adipose tissue, isolate adipocytes, process and re-inject stem and regenerative cells to the breast of the patient.

The invention relates to an acquisition method of adipose-derived stem cells. The method comprises the following specific steps of: digesting adipose tissues by use of mixed collagenase; culturing adipose-derived stem cells obtained through digestion in a culture solution; carrying out solution replacement and transfer of culture; and detecting to obtain the qualified adipose-derived stem cells. According to the invention, the problems that in the traditional cell separation method, cells can not be thoroughly digested by collagenase and the cell purity is low can be solved; and a separation method of adipose-derived stem cells is improved and optimized, so that tissues can be digested more thoroughly, and the purity during a culture process is higher, thus a more excellent seed cell source is ensured.

A tissue engineering cartilage construction method using bone matrix gelatin which comprises, using cartilage cell for isolated culture or base material stem cell for isolated culture to evoke cartilage cell i.e. seed cell, fetching New Zealand rabbit or human embryon os longum and metaphysic trabecular bone to construct bone matrix gel BMG, inoculating the seed cells harvested through isolated culture onto cortex bone or cancellous bone BMG for extracorporal culture.

The present invention provides a rare cell isolation device including: a first body which is disposed above a filtration membrane and includes a first inlet for injecting a biospecimen; and a second body which is disposed under the first body and bonded to the filtration membrane, wherein the first body and the second body have a disk-shaped structure to be rotatable around their centers, and the filtration membrane is disposed to be separated from the center of the second body in a radial direction.

The invention provides a separating method and an application of fat stem cells. The method provided by the invention comprises steps that: (1) an obtained fat cell tissue material containing fat stem cells is washed by using a washing liquid; (2) the material is digested by using collagenase, such that a digested tissue mixture is obtained; (3) the tissue mixture is filtered, such that a filtrate containing the fat stem cells is obtained; and (4) the filtrate is centrifuged, and an obtained precipitate is a fat stem cell mass. With the method provided by the invention, fat stem cells can be effectively separated; the purity of the obtained fat stem cells is high; and the proliferation speed of the fat stem cells is high. The fat stem cells separated by using the method provided by the invention can be applied in regeneration medicine, anti-aging treatments, and the like.

The invention relates to a centralized prison passageway access control system, which comprises a centralized access control system arranged on a prison gate and used for access to the prison gate, an access control system arranged on the door of a cell isolation area and used for access to the cell isolation area, an access control system arranged on the door of a cell building or floor and used for access to the cell building or floor, access control systems arranged on the doors of cells and used for access to the cells, an access control system arranged on the door of a prison office area and used for access to the prison office area, and an integrated information security platform. By using the centralized prison passageway access control system, the implementation of the daily supervision work of a functional department is more benefited, the ability of a prison in dealing with emergencies is further enhanced, and the control and management of incoming and outgoing people and all gates and doors by the prison are greatly enhanced.

A method for forming a memory cell and periphery circuit includes providing a substrate with a peripheral circuit region and a memory cell region. A mask layer is formed on the substrate to define multiple active regions in the peripheral circuit region and to define multiple channel regions in the memory cell region. Multiple field oxide layers are formed between the active areas, and Dopants are implanted in the substrate between the channel regions. Multiple inter-cell isolation layers are formed between the channel regions and the dopants are driven in the substrate to form buried diffusion regions. The mask layer is removed. A layer of electricity-storage material and multiple word lines are formed on the substrate in the memory cell region.

The invention provides a separation method for mouse female germline stem cells in the technical field of transgenosis engineering. The separation method comprises the following steps: step one, collecting a mouse ovary, and adopting a two-step enzymic digestion method for preparing germline stem cell suspension; and step two, adding mvh first antibody and then second antibody magnetic bead in the cell suspension and re-suspending the cells, thus separating the female germline stem cells. The invention also provides a culture in vitro method for the mouse female germline stem cells, which comprises the following steps: transferring the female germline cells on an STO cell culturing layer, adding female germline stem cell culture solution, and conducting primary culture and subculture on the female germline stem cells, thus obtaining purified and stable germline stem cells. In the method, the mouse female fermline stem cells are separated and cultured in vitro successfully at first time, the methods are simple, the cost is low, the requirements on experiment conditions are low, and the operability is strong.

The invention relates to the technical field of adipose-derived stem cell isolated culture, and discloses a dental pulp stem cell isolated culture method. The dental pulp stem cell isolated culture method comprises the following steps: washing adipose tissues, digesting the adipose tissues by using collagenase Type I, separating adipose stromal vascular fractions, then carrying out scavenging-precipitation, planting and culturing obtained primary cells to obtain the primary cells in the end, and carrying out primary cell passage. The dental pulp stem cell isolated culture method has the beneficial effects that by standardizing the dental pulp stem cell isolated culture method and various parameters in concrete operations, the adipose-derived stem cell isolated culture efficiency is improved, the output rate is increased, and the production cost is reduced.

The present invention relates to a method of isolating cells from a sample which method comprises binding said cells to a solid support by means of a non-specific ligand immobilised on said solid support, particularly to a method of isolating microorganisms from a sample. Preferred ligands for use in such methods include carbohydrates and derivatives thereof. Also described is a kit for isolating microorganisms from a sample comprising: (a) a solid support having immobilised thereon a ligand which is capable of non-specific binding to microorganisms; (b) means for binding microorganisms to said solid support; optionally (c) means for lysing said cells; and optionally (d) means for binding nucleic acid released from said lysed cells to a solid support.

The invention provides a preparation method of a targeted superparamagnetic nano-probe. The method includes the steps of: 1. preparing ferroferric oxide nano-particles with a particle size of 10-20nm in an oil phase by high temperature thermal decomposition; 2. synthesizing an amphiphilic polymer; 3. conducting aqueous phase transformation of the ferroferric oxide nano-particles obtained by step 1 through the amphiphilic polymer obtained by step 2 so as to obtain water-soluble superparamagnetic nano-particles; and 4. under the coupling effect of EDC/NHS, subjecting the water-soluble superparamagnetic nano-particles obtained by step 3 to covalent coupling with a target molecule, thus obtaining the targeted superparamagnetic nano-probe. The invention also provides an application method of the probe. Under the action of a separation column and an applied magnetic field, the probe can specifically and effectively capture and separate target cells. Compared with the prior art, the probe prepared by the method provided by the invention has the advantages of uniform particle size, high T2 relaxation rate, good stability, high pH value, high salt tolerance, and good target cell separation effect.

The invention provides a kit for detecting human regulatory T cell subtypes and a detection method and belongs to the technical field of cell subtype detection. The provided kit comprises components as follows: a blood diluent, a mononuclear cell separation medium, a cell culture fluid, a lymphocyte activation solution, dead cell removal dyes, an FcR blocking agent, a fluorescence labeled antibodyresisting human cell surface labeling molecules, a fluorescence labeled antibody resisting human intracellular molecules, a PBS buffer solution, lipopolysaccharide, a washing buffer solution, a celldyeing buffer solution, a cell fixing solution and a permeable membrane lotion. During detection, firstly, mononuclear cells in whole blood are separated, then, mononuclear cells of human peripheral blood are stimulated, finally, a fluorescent antibody is stained, and the regulatory T cell subtypes can be detected. By use of the kit and the detection method, 2-6 regulatory T cell subtypes can be simply and rapidly distinguished.

The invention discloses a single cell separation system and method based on droplet microfluidics. The system comprises a micro-fluidic chip, a microscope, a high-speed camera, a computer, a single-chip microcomputer and a plunger pump, wherein the high-speed camera captures an image of liquid drops under the microscope and transmits the image to the computer for image processing; the computer sends a sorting signal to the single-chip microcomputer; and the single-chip microcomputer controls the plunger pump to move, negative pressure is formed to deflect the liquid drops, and the liquid drops wrapping single cells are enabled to flow to a collection channel. According to the invention, liquid drop identification is carried out through a machine vision method; that is, the high-speed camera shoots liquid drop images in the channel of the micro-fluidic chip under the microscope, and image analysis is carried out to realize liquid drop identification. According to the method, cells do not need to be treated, so the cells are hardly damaged; operation is simple, an automation degree is high, and the single cells can be efficiently and quickly separated; and the problems that an automation degree is low, cells are easily damaged, flux is relatively low, operation is relatively tedious and the like in previous single cell separation processes are solved.

The application discloses a magnetic nano composite material, and a preparation method and application of the material, and belongs to the field of medical materials. The magnetic nano composite material comprises magnetic nanoparticles and a hydrophilic compound layer coated outside the magnetic nanoparticles, the magnetic nanoparticles are iron oxides, the particle size of the magnetic nanoparticles is 0.1-20 nm, the particle size of the magnetic nano composite material is 0.5-300 nm, and the longitudinal relaxation rate r1 of the magnetic nano composite is >= 20 mM-1s-1. The provided magnetic nano composite material can be used as a contrast material, and can be used in the aspects of magnetic resonance imaging contrast agents, targeted drugs, cell separation, etc.