318 results about "Cell isolation" patented technology

The invention provides a spiral structure inertia focusing microfluidic cell screening device and a screening method thereof.The spiral structure inertia focusing microfluidic cell screening device comprises a planar spiral structure, the planar spiral structure is a single-ring planar spiral structure, and a single-ring planar spiral structure channel in the planar spiral structure is provided with at least two inlet channels and at least three outlet channels; the single-ring plane spiral structure channel, the inlet channel and the outlet channel are rectangular cross section channels, the single-ring plane spiral structure is provided with a plurality of stabbing structures, and the injection pump is driven to adjust the flow of a blood sample and sheath flow liquid so as to adjust the starting point Reynolds number of the single-ring plane spiral structure channel, so that stable cell inertial focusing is realized; the device can replace a complex and high-cost multi-separation microfluidic device, is not easy to block, easy to clean, high in stability, not easy to deform, high in experiment accuracy and reliability, beneficial to long-term experiment operation and use, and high in repeated utilization rate.

The invention discloses a method for separating, sorting and concentrating rare cells. The method comprises the following steps: pretreating a to-be-separated sample, adding the pretreated sample into a sample accommodating assembly in the cell separation, sorting and concentration device, and controlling the sample accommodating assembly to separate, sort and enrich the to-be-separated sample through a filtering and sorting mechanism to obtain rare cells. The specific cell separating, sorting and concentrating device is designed, and the method for separating, sorting and concentrating the rare cells is developed based on the device, so that the recovery rate and concentration rate of the rare cells are improved, the operation is simplified, the smear operation process is further developed, the separation, sorting, enrichment and smear processes of the cells can be realized, and the production efficiency is improved. The cell loss is prevented, the smear quality is improved, and the smear time is shortened.

A method for fabricating a semiconductor device includes forming a stack body including a plurality of recess target layers over a lower structure; forming sacrificial isolation openings in the stack body; forming sacrificial isolation layers in the sacrificial isolation openings; forming sacrificial vertical openings having bottom surfaces disposed at a lower level than bottom surfaces of the sacrificial isolation openings in the stack body between the sacrificial isolation layers; forming preliminary horizontal layers by recessing the recess target layers through the sacrificial vertical openings; forming sacrificial pillar structures that fill the sacrificial vertical openings; forming cell isolation openings by removing the sacrificial isolation layers; forming horizontal layers by trimming the preliminary horizontal layers through the cell isolation openings; and forming cell isolation layers that fill the cell isolation openings.

The invention discloses a monoluciferase-based ADCP biological activity detection method, which comprises the following steps: separating a mononuclear cell from a human peripheral blood mononuclear cell as an effector cell, phagocytizing a Raji cell transfected by Luciferase as a target cell by the mononuclear cell, acidifying and degrading the Raji cell in a phagocytic body, and degrading the Luciferase so as to attenuate a light-emitting signal, so that the ADCP biological activity is detected. The ADCP biological activity of the antibody therapeutic drug is accurately quantified by detecting the weakening of the light-emitting signal. According to the present invention, the method only needs to separate the mononuclear cells and perform Luciferase infection on the target cells, and only needs to add the luciferase substrate after the phagocytosis process, such that the luminescence value can be read by using the microplate reader, the operation process is simple, the steps are less, the time consumption is short, the high-throughput screening is achieved, and the new direction is provided for the ADCP detection.

The invention discloses an isolated culture method of placenta decidua basalis stem cells. The isolated culture method comprises the following steps: acquiring decidua basalis tissues from a placenta; a trypsin solution is added into the bottom demolding tissue for pre-digestion, and a pre-digested bottom demolding tissue is obtained; adding a mixed enzymatic hydrolysate of trypsin and collagenase into the pre-digested decidua basalis tissue, carrying out digestion treatment under a magnetic stirring condition, sieving, centrifuging, and discarding supernate to obtain decidua basalis stem cells; adding a complete culture medium into decidua basalis stem cells, blowing, beating and uniformly mixing, and inoculating the decidua basalis stem cells into a culture dish according to the inoculation density of 2000-5000 cells / cm < 2 > for culturing to obtain P0-generation cells; carrying out subculture on the P0 generation cells; according to the separation culture method, external pollution is effectively reduced through refined cleaning and stripping steps, and then a one-step digestion method is adopted and combined with a magnetic stirring technology, so that the cell separation efficiency and purity are remarkably improved; meanwhile, the optimal inoculation density is explored through practice, and the cell culture effect and yield are directly improved.

The invention belongs to the technical field of cell biology, and particularly discloses a culture medium and a culture method of fish primary liver cells. The culture medium of the fish primary liver cells comprises the following components: 90% of an M199 culture medium, 10% of fetal calf serum, 100 U / mL of penicillin, 0.1 mg / mL of streptomycin, 5 ng / mL of EGF, 10 [mu] U / ml of insulin and 10 mM of dexamethasone. Based on the culture medium, an efficient and stable fish liver cell culture method is developed by combining a standardized anesthesia and disinfection process, a precise dissection and liver sampling method, an optimized tissue digestion and cell separation scheme, an efficient cell purification and adherent culture system, a stable passage technology and the like; a standardized hepatocyte line establishment operation process is defined, and the problems that species binding exists in an existing fish liver cell culture method, and a standardized culture process suitable for multiple fish fingerlings is difficult to form are solved.

The invention provides a method for culturing primary spermatogonial stem cells of Tibetan sheep, and belongs to the technical field of cell separation and culture. The culture method provided by the invention comprises the following steps: disinfecting and cleaning testis tissues of Tibetan sheep lambs; shearing tissue blocks, and digesting and cleaning to obtain a single-cell suspension of the seminiferous tubule; purifying the single-cell suspension, and culturing without a feeding layer or with the feeding layer; the culture without the feeding layer refers to culture in a laminin-coated culture container by using a feeding-layer-free MEM alpha culture medium or a StemProTM-34SFM complete culture medium; culture with a feeding layer refers to culture in a mouse fibroblast line feeding layer or a Tibetan sheep primary testicular sertoli feeding layer, and a feeding layer MEMS alpha culture medium is added. According to the culture method provided by the invention, the Tibetan sheep spermatogonial stem cells with higher purity can be obtained through separation, the subsequent proliferation of the spermatogonial stem cells can be remarkably promoted, and the apoptosis of the spermatogonial stem cells can be inhibited.

The cell separation element has a housing containing a fluid, which is formed by two foil-like housing parts. They are tightly connected to each other along their edges, with at least one integral connection extending along the length of the edge being provided in the edge region. This connection is covered by an elastically deformable cover part provided on the edge of the housing.

1. Name of the product of this design: Graphical user interface for operation control of blood separator for electronic equipment (XJ‑III). 2. Purpose of this design product: used in an electronic device. 3. The key design point of this design product lies in the content displayed in the graphical user interface. 4. The picture or photo that best illustrates the key points of the design: main view. 5. The electronic equipment is of conventional design and other views are omitted. 6. Purpose of the graphical user interface: The product interface is used to operate and control the blood separator. Through human-computer interaction, users can realize equipment self-test, plasma separation, red blood cell separation, PRP separation, consumables installation, information entry, parameter setting, plasma collection, etc. 7. Human-computer interaction mode of the graphical user interface: click "Device Self-Test" in the main view, the interface jumps to interface change state diagram 1; click "Confirm" in interface change state diagram 1, the interface jumps to interface change state diagram 2; click "Plasma Separation" in interface change state diagram 2, the interface jumps to interface change state diagram 3; click "Pre-charge" in interface change state diagram 3, the interface jumps to interface change state diagram 4; click "Next" in interface change state diagram 4, the interface jumps to interface change state diagram 5; click "Confirm" in interface change state diagram 5, the interface jumps to interface change state diagram 6; click "Stop" in interface change state diagram 6, the interface jumps to interface change state diagram 7. In addition, the cross in the interface represents replaceable text and / or numbers and / or letters and / or symbols.

A cell detachment apparatus that detaches cells placed on a culture surface of a culture container for cells by applying vibration to the cells includes a vibrator and a vibrating plate, wherein the vibrator, the vibrating plate, an acoustic transmission medium, and the culture container are stacked in this order, wherein a bending vibration is excited on the culture container when a vibration of the vibrating plate is transmitted to the culture container via the acoustic transmission medium, and wherein the acoustic transmission medium and the culture container come into contact with each other at a region including an antinode of the bending vibration and do not come into contact at a node of the bending vibration.

An integrated circuit device includes a first cell and a second cell apart from each other in a first lateral direction on a substrate, the first cell and the second cell each including a plurality of gate lines, an inter-cell isolation region between the first cell and the second cell, the inter-cell isolation region extending in a second lateral direction, a power line including portions overlapping a cell boundary of each of the first cell and the second cell in a vertical direction and a portion overlapping the inter-cell isolation region in the vertical direction, a plurality of dummy gate insulation lines, a bridge insulating pattern in contact with an end portion of each of the plurality of dummy gate insulation lines, and a via power rail passing through the bridge insulating pattern, the via power rail being connected to the power line.

A cell processing platform and method for selecting and isolating target cells, such as T-cells, NK-cells, or hematopoietic stem cells, from a biological sample using functionalized microbubbles. The platform employs microbubbles with lipid shells, functionalized with streptavidin and biotinylated linkers, to bind specific target cells and render them buoyant within the suspension. A centrifugation module separates non-target cells by sedimentation while maintaining the buoyancy of target cell-microbubble complexes. A pneumatic control system disrupts the microbubbles under controlled pressure or frequency, releasing the target cells for downstream processing without compromising viability. Additional features include a microbubble generation module capable of sequentially functionalizing ligands for multi-step cell selection and a temperature-controlled environment to ensure ligand stability. The system supports non-destructive cell release and sorting, enabling precise, contamination-free, and high-efficiency cell isolation for therapeutic and research applications.

The present invention relates to an automatic cell block preparation machine and method. The automatic cell block preparation machine comprises a main body and a centrifugal component, a temperature control component, a mechanical arm, a liquid circuit system and a desktop host arranged on the main body. Based on the cell block preparation machine platform, cells in a cytological specimen are separated and enriched on a plane through the centrifugal action of a cell block preparation tube and a matrix, and are automatically prepared into cell blocks, thereby realizing the automation, standardization and high quality of cell block preparation.

The invention provides a kit for detecting an AChR antibody based on a CBA method. The kit comprises skeletal muscle primary cells of fetal mice and skeletal muscle primary cells of adult mice. The skeletal muscle primary cells of fetal rats and adult rats obtained through primary cell isolation culture can naturally express fetal and adult AChR target antigens respectively, and compared with a traditional cell transfection technology, the skeletal muscle primary cells of fetal rats and adult rats obtained through the method have natural AChR protein expression, and can be used for preparing the skeletal muscle primary cells of fetal rats and adult rats. The problems of high cytotoxicity and low detection sensitivity caused by transient expression of five subunits and polymeric protein in traditional cell transfection are successfully avoided, and the detection sensitivity of the AChR antibody is effectively improved.

The invention relates to a micro-fluidic chip for chemiluminescence imaging detection of single-cell secretions and a use method of the micro-fluidic chip. The micro-fluidic chip is composed of a single cell analysis layer and a glass substrate layer. The single cell analysis layer is prepared from polydimethylsiloxane and is provided with a capture channel, a sample introduction channel and a lantern-shaped microstructure array, the lantern-shaped microstructure comprises a small-size cell capture trap and a large-size chemiluminescence detection chamber, the small-size cell capture trap is connected with the capture channel, and the large-size chemiluminescence detection chamber is connected with the sample introduction channel. After a mixed solution of cells and a detection reagent is introduced into the capture channel, single cell capture is realized on the capture trap due to flow resistance difference; then, introducing air into the capture channel and the sample introduction channel to form a gas-in-liquid microcavity, and carrying out single cell isolation and incubation reaction; finally, the signal switch molecules and the substrate liquid are sequentially injected into the chemiluminescence detection chamber through the sample injection channel, and quantitative detection is conducted on the single-cell secretions according to chemiluminescence imaging signals.

The invention aims to provide a method for effectively separating and proliferating stem cells from peripheral blood. The composition according to the present invention comprises interferon-gamma (IFN-gamma) in order to enhance the adhesion of stem cells isolated from peripheral blood and thus improve the acquisition rate of stem cells. Therefore, the composition and the peripheral blood-derived stem cell isolation and culture method according to the present invention have been established for inducing progenitor cells differentiated into somatic cells that can be differentiated into various target tissues, and for preventing or treating diseases.

The invention discloses a cell separation device for blood cell detection and a use method thereof, and belongs to the technical field of blood cell detection.The cell separation device is provided with a rotating disc, an adjusting screw rod, a movable adjusting plate, a fixed bottom plate, a supporting bottom frame, a rotating frame and a mounting clamping ring, and the movable adjusting plate moves up and down on the surface of a mounting cylinder to drive the rotating frame to rotate; a mounting clamping ring is pulled to obliquely rotate, so that the placing mechanisms and the test tubes incline around the connecting positions of the placing mechanisms and the test tubes and the supporting bottom frame, and the six placing mechanisms and the test tubes are controlled to incline by the same angle in the centrifugal machine box, so that the test tubes are conveniently inclined by a specific angle according to cell separation requirements, the area of a separation interface is increased, and the thickness of a cell layer is reduced; cell layers are prevented from being mixed, so that the separation effect is improved, the inclination angle of the test tube during rotary centrifugation is conveniently controlled, and the situation that the test tube shakes due to centrifugal force during rotation, and cell sap subjected to centrifugal separation in the test tube is mixed is avoided.

The present invention relates to compositions suitable for cell isolation comprising a polypeptide having protease activity. The invention further relates to polypeptides having protease activity, polynucleotides encoding the polypeptides, the present invention relates to polynucleotides, nucleic acid constructs and expression vectors comprising the polynucleotides, recombinant host cells comprising the nucleic acid constructs or expression vectors, methods of producing the polypeptides, particles comprising the polypeptides, fermentation broth formulations comprising the polypeptides, and methods and uses employing the polypeptides.

Affinity-based and activity-based probes (ABPs) described herein offer transformative resolutions to microbiome function. These ABPs target key metabolic pathways in the gut microbiome. The ABPs contain a binding group, a reactive group, and a. reporter group handle that allows for the addition of a "flexible" reporter group that can be easily swapped to enable multimodal fluorescence and proteomic measurements and isolation of live cells. The binding group, also called an affinity element or biorecognition element, mirrors monomeric and polymeric carbohydrates, sulfated and acetylated carbohydrates, and peptides to afford probe selectivity.

The invention relates to a cell separating element, having a housing (1) in which a fluid is provided and which is formed by two film-like housing parts (2, 3). These are connected to one another in a sealingly fixed manner along their edge (4, 5), wherein at least one integral connection (13) running over the length of the edge (4, 5) is provided in the edge region. Said connection is covered by an elastically deformable cover part (14) which is provided on the edge (4, 5) of the housing.

The invention relates to the technical field of cell separation equipment, in particular to a pipe clamping mechanism and a liquid road faceplate, the pipe clamping mechanism comprises at least one telescopic assembly, a mounting plate and a cover plate, the mounting plate is used for mounting the telescopic assembly, and the extending end of the telescopic assembly can be close to or away from the cover plate to clamp or loosen a hose; the liquid path panel is matched with the pipe clamping mechanism to tightly jack or loosen the hose, so that the hose is convenient to disassemble and assemble, and the flowing condition of a liquid path can be directly observed.

The invention provides a rabbit-source BCR sequence primer group based on high-throughput single cell V (D) J sequencing and application of the rabbit-source BCR sequence primer group. The primer group comprises a specific nested PCR (Polymerase Chain Reaction) primer pair (the sequence is as shown in SEQ ID NO.1-12) aiming at a rabbit BCR heavy chain (IgA / IgE / IgG / IgM) and a light chain (IgK / IgL). Through microfluid single-cell separation, nested PCR amplification and high-throughput sequencing, the naturally paired rabbit BCR full-length V (D) J sequence can be efficiently obtained. According to the rabbit-derived BCR sequence sequencing primer provided by the invention, B cell receptor library information of different tissue sources can be quickly and efficiently obtained. According to the method, paired antibody sequence information can be specifically obtained, B cell response diversity after rabbit immunization vaccine or pathogen infection can be better known, and important data support is provided for design, optimization, effect evaluation and the like of biological products.

The utility model belongs to the field of centrifuge tubes, and particularly relates to a multifunctional centrifuge tube convenient for cell separation and purification, which comprises a tube body and a separation and purification net, the outer side of an opening at the upper end of the tube body is connected with a tube cover through threads, the separation and purification net is positioned on the inner side of the tube body, and the top of the separation and purification net is fixedly connected with a backing ring. A backing ring is arranged in the pipe body and makes contact with the top of the pipe body, a flange is fixedly connected to the outer wall of the pipe body, a clamping pin is slidably connected to the interior of the pipe cover, the clamping pin is connected with the flange in a clamped mode, and a spring is arranged in the pipe cover. The separating and purifying net with the backing ring is arranged in the tube body and used for separating and purifying cells, the functionality of the centrifugal tube is improved, meanwhile, the flange is arranged on the outer side of the tube body, the clamping pin stretching out and drawing back through the spring is arranged in the tube cover, and the clamping pin can be clamped into the flange under the action of the spring, so that the cell separation and purification effect is improved. Therefore, the tube cover has a certain anti-loosening effect, so that the tube cover is not easy to loosen during centrifugation.

To provide: a cell culture vessel that can achieve both detachability and proliferation ability, that is, can facilitate cell detachment without reducing the proliferation ability of cells; and a cell detachment method using the cell culture vessel, provided is a cell detachment method of detaching, through use of a culture vessel having a periodic recess / protrusion pattern on a culture surface, comprising a detachment step of: causing a vibrating unit in which, when an observation image of a cross section perpendicular to the culture surface is obtained, a length Lcp of a contour of the cell on the culture surface side in a measurement region of the observation image and a length Lf of the measurement region in a direction parallel to the culture surface satisfy Lcp>Lf; and transmitting the ultrasonic vibration to the cell, to thereby detach the cell from the culture surface.

A reactor apparatus comprising a reactor vessel 32, media conditioning circuit 34 and a fluid flow controller 36 wherein the fluid flow controller allows the flow of fluid through the reactor vessel to be reversed. The external fermentation broth loop has conditioning modules 50 52 that may alter pH, temperature, gases, or remove waste products. The fluid flow controller may reverse the flow automatically at intermittent periods, and may be a pump or valve. In an alternative configuration, there is a media and cell separation device 42 44 that retains cells inside the reactor vessel, allowing circulation of reaction media between the bioreactor and media loop. The cells may be separated from the media through a filter or membrane, or mechanically separated by gravity or centrifugation. Additionally, there may be several reactor vessels that are connected in a cascading (430, Fig 5) or parallel (330, Fig 5) arrangement. A method of operating the reactor apparatus is also disclosed.

1. The name of this design product: centrifuge. 2. Purpose of the product with this design: This product with this design is a special equipment used for cell separation, microorganism separation and blood separation. 3. The key point of the design of this product lies in its shape. 4. The picture or photo that best illustrates the design points: Stereoscopic drawing 1.

The invention is suitable for the technical field of cell separation and culture, and provides a pig nasal mucosa epithelial cell separation and culture method. A mixed enzyme overnight digestion method is adopted, mucous membrane tissue mucus is effectively removed, and more complete digestion is achieved. The pig nasal mucosa epithelial cells with relatively high purity are obtained through a differential attachment technology. And the culture medium is replaced by a complete culture medium containing EGF and vitamin A during first liquid replacement, so that a stable and excellent in-vitro growth environment is provided for epithelial cells. The method not only can rapidly and accurately separate the primary cells, but also ensures the stability and high quality of the culture state of the epithelial cells. According to the method, the blank of porcine nasal mucosa primary cell separation research is filled up, the primary porcine nasal mucosa epithelial cell in-vitro model which is simple and easy to implement and high in repeatability is constructed, and the model can simulate physiological and morphological characteristics of porcine nasal mucosa and can be used for preparing the porcine nasal mucosa epithelial cell in-vitro model. And a reliable in-vitro experimental basis is provided for researches on respiratory tract pathogen infection mechanisms, respiratory system disease drug screening, gene therapy and the like.

A method of isolating mature adipocytes from a tissue is disclosed. The disclosed method comprises incubating the tissue in a solution comprising an amphiphilic agent and an enzyme. The amphiphilic agent may be Pluronic F-68 and the enzyme may be collagenase. Methods of culturing mature adipocytes comprising culturing mature adipocytes isolated by one of the methods disclosed herein and methods of differentiating fat cells comprising culturing mature adipocytes according to any one of the methods disclosed herein are also disclosed. A kit comprising an amphiphilic agent and an enzyme is disclosed.

The utility model provides a cell separation device and an analysis device based on magnetic nanoparticles, and belongs to the technical field of biomedicine. The separation device comprises a cell and magnetic nanoparticle incubation unit, a cell separation unit and a cell collection unit. Target cells are accurately captured mainly through magnetic nanoparticles with antibodies and are accurately separated out through magnetic field adsorption force of an electromagnetic coil. According to the separation device and the analysis device, the whole multi-channel design in the cell incubation, separation, collection and analysis processes simultaneously realizes the treatment of a plurality of samples and the synchronous separation of different cells, and the accuracy of cell separation is improved through accurate magnetic field control and magnetic nanoparticles. The requirements on high throughput and high precision of cell separation at present are met.

The invention belongs to the technical field of in-vitro cultured calculus bovis, and provides in-vitro cultured calculus bovis and a preparation method thereof. The preparation method comprises the following steps: (1) separating cells; (2) primary culture; (3) cell inoculation and cultivation: adding the serum-free culture medium into the carrier matrix, putting the carrier matrix into a reactor, and performing cell inoculation on the carrier matrix after passage; the inoculated culture comprises a proliferation period; a synthesis period; a crystallization period; and (4) collecting and purifying the calculus bovis, namely stopping culturing after 45 days, and sequentially collecting, cleaning, degreasing, drying and crushing the calculus bovis. The preparation method aims at solving the problems of long culture period, unstable content of effective components, high production cost, large batch difference and the like in the existing in-vitro calculus bovis culture technology by optimizing a culture medium formula, regulating and controlling a culture process and a purification process.