111 results about "Cell isolation" patented technology

The invention belongs to the technical field of cell biology, and particularly discloses a culture medium and a culture method of fish primary liver cells. The culture medium of the fish primary liver cells comprises the following components: 90% of an M199 culture medium, 10% of fetal calf serum, 100 U / mL of penicillin, 0.1 mg / mL of streptomycin, 5 ng / mL of EGF, 10 [mu] U / ml of insulin and 10 mM of dexamethasone. Based on the culture medium, an efficient and stable fish liver cell culture method is developed by combining a standardized anesthesia and disinfection process, a precise dissection and liver sampling method, an optimized tissue digestion and cell separation scheme, an efficient cell purification and adherent culture system, a stable passage technology and the like; a standardized hepatocyte line establishment operation process is defined, and the problems that species binding exists in an existing fish liver cell culture method, and a standardized culture process suitable for multiple fish fingerlings is difficult to form are solved.

An integrated circuit device includes a first cell and a second cell apart from each other in a first lateral direction on a substrate, the first cell and the second cell each including a plurality of gate lines, an inter-cell isolation region between the first cell and the second cell, the inter-cell isolation region extending in a second lateral direction, a power line including portions overlapping a cell boundary of each of the first cell and the second cell in a vertical direction and a portion overlapping the inter-cell isolation region in the vertical direction, a plurality of dummy gate insulation lines, a bridge insulating pattern in contact with an end portion of each of the plurality of dummy gate insulation lines, and a via power rail passing through the bridge insulating pattern, the via power rail being connected to the power line.

The invention relates to a micro-fluidic chip for chemiluminescence imaging detection of single-cell secretions and a use method of the micro-fluidic chip. The micro-fluidic chip is composed of a single cell analysis layer and a glass substrate layer. The single cell analysis layer is prepared from polydimethylsiloxane and is provided with a capture channel, a sample introduction channel and a lantern-shaped microstructure array, the lantern-shaped microstructure comprises a small-size cell capture trap and a large-size chemiluminescence detection chamber, the small-size cell capture trap is connected with the capture channel, and the large-size chemiluminescence detection chamber is connected with the sample introduction channel. After a mixed solution of cells and a detection reagent is introduced into the capture channel, single cell capture is realized on the capture trap due to flow resistance difference; then, introducing air into the capture channel and the sample introduction channel to form a gas-in-liquid microcavity, and carrying out single cell isolation and incubation reaction; finally, the signal switch molecules and the substrate liquid are sequentially injected into the chemiluminescence detection chamber through the sample injection channel, and quantitative detection is conducted on the single-cell secretions according to chemiluminescence imaging signals.

Affinity-based and activity-based probes (ABPs) described herein offer transformative resolutions to microbiome function. These ABPs target key metabolic pathways in the gut microbiome. The ABPs contain a binding group, a reactive group, and a. reporter group handle that allows for the addition of a "flexible" reporter group that can be easily swapped to enable multimodal fluorescence and proteomic measurements and isolation of live cells. The binding group, also called an affinity element or biorecognition element, mirrors monomeric and polymeric carbohydrates, sulfated and acetylated carbohydrates, and peptides to afford probe selectivity.

The invention relates to the technical field of cell separation equipment, in particular to a pipe clamping mechanism and a liquid road faceplate, the pipe clamping mechanism comprises at least one telescopic assembly, a mounting plate and a cover plate, the mounting plate is used for mounting the telescopic assembly, and the extending end of the telescopic assembly can be close to or away from the cover plate to clamp or loosen a hose; the liquid path panel is matched with the pipe clamping mechanism to tightly jack or loosen the hose, so that the hose is convenient to disassemble and assemble, and the flowing condition of a liquid path can be directly observed.

The invention provides a rabbit-source BCR sequence primer group based on high-throughput single cell V (D) J sequencing and application of the rabbit-source BCR sequence primer group. The primer group comprises a specific nested PCR (Polymerase Chain Reaction) primer pair (the sequence is as shown in SEQ ID NO.1-12) aiming at a rabbit BCR heavy chain (IgA / IgE / IgG / IgM) and a light chain (IgK / IgL). Through microfluid single-cell separation, nested PCR amplification and high-throughput sequencing, the naturally paired rabbit BCR full-length V (D) J sequence can be efficiently obtained. According to the rabbit-derived BCR sequence sequencing primer provided by the invention, B cell receptor library information of different tissue sources can be quickly and efficiently obtained. According to the method, paired antibody sequence information can be specifically obtained, B cell response diversity after rabbit immunization vaccine or pathogen infection can be better known, and important data support is provided for design, optimization, effect evaluation and the like of biological products.

The utility model belongs to the field of centrifuge tubes, and particularly relates to a multifunctional centrifuge tube convenient for cell separation and purification, which comprises a tube body and a separation and purification net, the outer side of an opening at the upper end of the tube body is connected with a tube cover through threads, the separation and purification net is positioned on the inner side of the tube body, and the top of the separation and purification net is fixedly connected with a backing ring. A backing ring is arranged in the pipe body and makes contact with the top of the pipe body, a flange is fixedly connected to the outer wall of the pipe body, a clamping pin is slidably connected to the interior of the pipe cover, the clamping pin is connected with the flange in a clamped mode, and a spring is arranged in the pipe cover. The separating and purifying net with the backing ring is arranged in the tube body and used for separating and purifying cells, the functionality of the centrifugal tube is improved, meanwhile, the flange is arranged on the outer side of the tube body, the clamping pin stretching out and drawing back through the spring is arranged in the tube cover, and the clamping pin can be clamped into the flange under the action of the spring, so that the cell separation and purification effect is improved. Therefore, the tube cover has a certain anti-loosening effect, so that the tube cover is not easy to loosen during centrifugation.

To provide: a cell culture vessel that can achieve both detachability and proliferation ability, that is, can facilitate cell detachment without reducing the proliferation ability of cells; and a cell detachment method using the cell culture vessel, provided is a cell detachment method of detaching, through use of a culture vessel having a periodic recess / protrusion pattern on a culture surface, comprising a detachment step of: causing a vibrating unit in which, when an observation image of a cross section perpendicular to the culture surface is obtained, a length Lcp of a contour of the cell on the culture surface side in a measurement region of the observation image and a length Lf of the measurement region in a direction parallel to the culture surface satisfy Lcp>Lf; and transmitting the ultrasonic vibration to the cell, to thereby detach the cell from the culture surface.

A method of isolating mature adipocytes from a tissue is disclosed. The disclosed method comprises incubating the tissue in a solution comprising an amphiphilic agent and an enzyme. The amphiphilic agent may be Pluronic F-68 and the enzyme may be collagenase. Methods of culturing mature adipocytes comprising culturing mature adipocytes isolated by one of the methods disclosed herein and methods of differentiating fat cells comprising culturing mature adipocytes according to any one of the methods disclosed herein are also disclosed. A kit comprising an amphiphilic agent and an enzyme is disclosed.

The application provides a back contact cell isolation glue composition and a back contact cell isolation glue. The back contact cell isolation glue composition comprises, in percentage by mass, 60-84% of a resin base material, 10-30% of an active diluent monomer, 0.5-2% of a filler and 2-8% of a photoinitiator; wherein the resin base material comprises silicone acrylate, acrylic monomer and polyurethane modified acrylate; the mass percentage content of amino groups in the polyurethane modified acrylate is 10-30%. The above technical scheme adjusts the proportion of the resin formula, ensures that the isolation glue keeps low yellowing and high light transmittance for a long time, and guarantees the power output and appearance stability of the photovoltaic module. The silicone acrylate makes the thermal decomposition temperature of the isolation glue reach 300-400 DEG C, meets the high-temperature edge passivation process, realizes continuous protection in the whole process of cell manufacturing, and solves the protection problem of the cell piece in the high-temperature process.

The utility model discloses a rapid mononuclear cell separation tube capable of being easily filled with a partition plate, and belongs to the technical field of cell separation, the rapid mononuclear cell separation tube comprises a centrifugal tube and the partition plate provided with three supporting legs, the partition plate is provided with a peripheral through hole, and the side wall of the bottom in the centrifugal tube is provided with a flange surrounding the axis of the centrifugal tube; a supporting cover is arranged at the open end of the centrifugal tube, a supporting rod moving in the axis direction of the centrifugal tube is arranged on the supporting cover, and a tray used for supporting the partition plate and driving the partition plate to move relative to the centrifugal tube in parallel is arranged at the end, located in the centrifugal tube, of the supporting rod. The tray is provided with a protrusion matched with the peripheral through hole, the protrusion is provided with an air hole which is the same as the peripheral through hole and penetrates through the tray, and the device has the advantages that the partition plate can be rapidly and accurately filled under the condition that it is guaranteed that the partition plate is relatively parallel to the inner diameter of the centrifugal tube, the filling efficiency is greatly improved, and the partition plate and the centrifugal tube are firmly fixed.

A cell detachment method for detaching a cell from a substrate by applying vibration to the cell adherent to the substrate includes an incubation step of incubating the cell after bringing a cell detachment liquid containing substantially no proteolytic enzyme into contact with the cell, an information acquisition step of acquiring information relating to the incubated cell, a determination step of determining a time when vibration is applied to the incubated cell in accordance with the information relating to the cell, and a detachment step of applying vibration to the incubated cell.

The invention relates to the technical field of biomedical processing, and particularly provides a cryopreservation method of cord blood mononuclear cells, which comprises six steps of whole blood separation, plasma inactivation, CBMC cell separation, cryopreservation liquid preparation, cell cryopreservation and detection after cryopreservation recovery. The CBMC cell separation comprises three sub-steps; performing cell cryopreservation, namely averagely dividing the separated CBMC into a plurality of parts, filling the CBMC into a cryopreservation tube according to a certain density, putting the cryopreservation tube into a programmed cooling instrument, and performing cryopreservation according to a plurality of programs; and detecting after cryopreservation and resuscitation, wherein the detection comprises three sub-steps of cell viability detection, cell recovery rate detection and cell function detection. The problems that in the cryopreservation process of a traditional method, cell viability is reduced, the number of cells is greatly reduced, cell adhesion and caking are caused, cell functions are abnormal, cell phenotypes are changed after recovery, functional activity is damaged and the like are solved, the utilization rate of the CBMC is increased, and the technical scheme is obviously superior to the traditional cell cryopreservation method; the viability, the recovery rate and the function of the recovered cells are obviously improved.

The application discloses a kind of mitochondrial quality control detection methods, the present application relates to mitochondrial quality control detection technical field, operating steps include preparation sample cell suspension, fluorescent dye labeling, cardiomyocyte separation, establish myocardial cell damaged model and detect mitochondrial membrane potential situation.This mitochondrial quality control detection method can simultaneously carry out multi-parameter, rapid quantitative analysis and sorting, measure fast, measure the multi-parameter characteristics of each cell, while carrying out cell characteristic analysis, can separate out the cell of specified characteristics, so as to further culture, cloning, observation or carry out mitochondrial fragmentation test to specific cell, establish myocardial cell damaged model using doxorubicin, the mitochondrial membrane potential situation of normal myocardial cell and damaged myocardial cell obtained by using JC-1 mitochondrial membrane potential fluorescent probe detection is verified the detection quality of this mitochondrial quality control detection method.

The present invention relates to use of a polypeptide having a sequence identity of at least 70% to SEQ ID NO:1, a mature polypeptide of SEQ ID NO:1, SEQ ID NO:2, or SEQ ID NO:3, wherein the polypeptide has chymopapain activity, in a cell detachment process. The present invention also relates to a method of cell detachment, comprising contacting a cell with a polypeptide having a sequence identity of at least 70% to SEQ ID NO:1, a mature polypeptide of SEQ ID NO:1, SEQ ID NO:2, or SEQ ID NO:3, wherein the polypeptide has chymopapain activity, and wherein the cell is attached to a surface and / or to another cell. The present invention further relates to compositions suitable for cell detachment comprising said polypeptide, polynucleotides encoding said polypeptide, recombinant host cells transformed with said polypeptide, and methods of producing said polypeptide.

This invention discloses a method for preparing and applying nanocluster magnetic beads, comprising the following steps: adding a salt solution containing ferrous and ferric salts to an aqueous solution, dissolving it uniformly, then adding a dispersant to obtain a mixed solution, stirring evenly, and heating; adding ammonia water to the heated mixed solution, stirring the reaction to obtain nanocluster magnetic beads. This invention directly synthesizes nanocluster magnetic beads using an innovative one-step method, eliminating the need for multi-step synthesis and subsequent separation steps, and the synthesized nanocluster magnetic beads can be used for cell separation and MRI imaging.

The invention discloses a blood cell separation magnet device and method, and belongs to the technical field of cell separation. The magnet device comprises a plurality of pole heads which are symmetrically arranged, and runway coils are arranged outside the pole heads; the central axis position of a gap defined by the runway coils is a working area, the working area is a uniform magnetic field capturing area, and magnetized target cells in blood cells are stably separated by using a uniform magnetic field; the width of the working area ranges from 8 mm to 15 mm, and the length of the working area ranges from 200 mm to 500 mm. Through cooperation of the runway coil and the pole head, a linear or planar working area which is small in space body, high in strength, large in gradient and uniform in magnetic field distribution is formed, and compared with an existing point-shaped or small-area high gradient, magnetized cells flowing through a sample in the area can be captured more efficiently and more thoroughly, and the recovery rate and the purity are remarkably improved.

Devices and methods suitable for cell separation. The devices herein include non-random voids interconnected through non-random pores and / or non-random solid geometrical structures optionally connected through solid non-random interconnecting elements. Such devices are preferably suitable for affinity-based cell isolation techniques which rely upon binding interactions.

In one aspect, the disclosure relates to a method for neuromodulation in a target, wherein the target can be an isolated plurality of cells, an isolated organ, or an animal subject In an aspect, the method includes at least the step of delivering X-ray radiation to one or more neurons in the target, wherein the one or more neurons express dTRPA1(A)10b, and wherein the X-ray radiation generates H2O2 via radiolysis of water. In a further aspect, the H2O2 can increase intracellular calcium ions which can, in turn, stimulate the release of neurotransmitters. Also disclosed are treatments of diseases, disorders, and symptoms thereof using the disclosed method.

The present application relates to the technical field of CAR-T cell treatment, and particularly relates to a method for preparing universal CAR-T cells and application thereof. The VH domain and VL domain of the beta2M binding molecule provided in the present application contain CDR sequences, and can specifically bind to beta2M. The binding molecule is added to CAR-T cells, and the binding molecule can specifically bind to CAR-T cells without beta2M gene knockout, so as to separate CAR-T cells with beta2M gene knockout from CAR-T cells without beta2M gene knockout, and realize purification of universal CAR-T cells.

The invention relates to a system, comprising: a) a sample processing unit, comprising an input port and an output port coupled to a rotating container having at least one sample chamber, the sample processing unit configured provide a first processing step to a sample or to rotate the container so as to apply a centrifugal force to a sample deposited in the chamber and separate at least a first component and a second component of the deposited sample; and b) a sample separation unit coupled to the output port of the sample processing unit, the cell separation unit comprising separation column holder, a pump and a plurality of valves configured to at least partially control fluid flow through a fluid circuitry and a separation column positioned in the holder, the separation column configured to separate labeled and unlabeled components of sample flowed through the column.

1. Name of the design product: bracelet (blood cell separation sheet). 2. Use of the design product: the design product is a bracelet worn on the wrist, which activates health conditions naturally through physical therapy in a non-drug and non-chemical way. 3. Design points of the design product: in the combination of shape and pattern. 4. Picture or photo best indicating the design points: perspective view 1.

This invention relates to the field of biomedical technology and discloses a cell separation and dispensing method, apparatus, and storage medium. The method involves separating the sample solution and separation solution injected into a first centrifuge container to obtain an intermediate product solution, which is then output to an intermediate product container. The first centrifuge container is then switched to a second centrifuge container, and the washing solution and all the intermediate product solution from the intermediate product container are input into the second centrifuge container. The mixed washing solution is then concentrated to obtain a concentrated product solution. Finally, the resuspension is input into the second centrifuge container, and the mixed resuspension is dispensed to obtain the target product. This invention utilizes the first centrifuge container for the separation process and the second centrifuge container for the concentration and dispensing processes, eliminating the need to wash the first centrifuge container, avoiding the risk of cell contamination, and improving processing efficiency and safety.

The utility model provides a cell separating, flaking and dyeing all-in-one machine. The cell separation, sheet preparation and dyeing all-in-one machine comprises a shell; the cleaning device comprises a shell and two mounting ports, the two mounting ports are formed in the positions, located at the bottom, of the back face of the shell, a cleaning fluid frame is mounted in one mounting port, a row of first butt-joint ports are formed in the top of the cleaning fluid frame, a protective shell is mounted on the back face of the cleaning fluid frame, and a miniature conveying pump is mounted in the protective shell. A conveying pipe is mounted at an outlet of the micro conveying pump, a suction pipe is mounted at an inlet of the micro conveying pump, and a waste liquid frame is mounted in the other mounting opening; the test tube table is mounted at the position, close to one side, of the bottom of the inner wall of the shell. According to the cell separation, flaking and dyeing all-in-one machine provided by the utility model, the dyeing needle can be automatically cleaned after being used through the design, so that the dyeing needle does not need to be frequently replaced, and the operation efficiency of the cell separation, flaking and dyeing all-in-one machine is favorably improved.

Described herein is a reversible aptamer selection technology for production-scale isolation of label-free cells (e.g., CD8+ T cells). Provided herein are methods for isolating a cell of interest from a biological sample by contacting the biological sample with an aptamer that specifically binds a cell surface marker specific for the cell of interest; separating the aptamer-bound cells from cells not bound to the aptamer; and recovering a cell of interest by disrupting binding of the aptamer to the cell surface marker.

The utility model belongs to the technical field of microbiological devices, and particularly relates to a cell separation box with a rotating shielding function, which comprises a box body, a rotating shielding part and a rotating shielding part, the rotary table is rotationally arranged in the box body and is provided with a plurality of accommodating positions for placing reaction tubes; the temporary storage position is arranged on one side of the rotary table, a plurality of reaction tubes are stacked in the temporary storage position, and each reaction tube is provided with at least one reaction chamber; wherein the number of the reaction chambers of each reaction tube is sequentially increased from bottom to top, and the volume of the reaction chambers of each reaction tube is sequentially decreased from bottom to top; according to the cell separation box with the rotary shielding function, the temporary storage position is arranged, and a plurality of reaction tubes with different specifications are stored in the temporary storage position, so that a user can select the reaction tubes with the required specifications according to requirements for cell separation; meanwhile, the reaction tube in the accommodating position abuts against the annular guide rail, and the accommodating position is driven by the rotary table to move along the annular guide rail so that the reaction tube can protrude at the operation window.

The utility model relates to the technical field of centrifugal tubes, in particular to a trap type centrifugal tube for primary cell separation. According to the technical scheme, the centrifugal tube comprises a centrifugal tube main body, wherein the centrifugal tube main body is sequentially provided with a thick tube part, a conical part and a thin tube part from top to bottom; the thin pipe part is provided with a plurality of prefabricated fractures in an array mode in the height direction of the thin pipe part; the conical part is used for communicating the thick pipe part with the thin pipe part, and the section of the conical part is funnel-shaped; a thin tube bottom with a semi-arc-shaped section is arranged at the bottom of the thin tube part; supporting side wings arranged on the outer wall of the thin pipe part in an array mode are arranged between the prefabricated fractures of each layer. And the sizes of the supporting side wings at the lower end of the thin tube part are gradually reduced. The cell culture device is relatively simple and convenient to operate, needs little time, does not need extra solution or antigen, and improves and avoids influence of non-culture medium solution on cells.

The utility model relates to the technical field of biology, in particular to a U-shaped tube cell separation device which comprises a bottom plate, a top plate is arranged at the upper end of the bottom plate, a negative pressure extraction mechanism is arranged on one side, located on the top plate, of the bottom plate, a lifting mechanism is arranged on the other side of the bottom plate, a containing groove is formed in the top plate, and a U-shaped cell filter tube is arranged in the containing groove. And the bottom of the U-shaped cell filter tube is supported by a lifting mechanism. The U-shaped tube cell separation device has the advantages of being simple in structure, convenient to operate, high in cell separation efficiency, small in cell damage and the like, can be suitable for separation of various types of cells through different microfiltration membranes, and meets the requirements of the fields of biological experiments, cell therapy and the like.

The utility model provides an immune cell filtering device, which relates to the technical field of cell filtering, and comprises a first filtering box, the surface of the first filtering box is fixedly connected with a pull plate, the bottom of the inner wall of the first filtering box is provided with a vertical rod, the bottom and the surface of the first filtering box are provided with first filtering grooves, and the first filtering grooves are provided with second filtering grooves. And first filter cloth is fixedly connected to the interior of the first filter tank. According to the utility model, cells are placed in the third filter box and are efficiently filtered through the filter cloth with different pore diameters, and meanwhile, the cells with different diameters can be separated according to the detachable filter box, so that the cell separation efficiency is improved, a user can conveniently use the cell separation device, and the cell separation device is convenient to use due to the arrangement of the propping sleeve. The second filtering box and the third filtering box can be conveniently and efficiently fixed, and the filtering stability and efficiency of the second filtering box and the third filtering box are improved.

The application discloses a disposable cell separation device, which comprises a transfer container, a rotary joint and a separation container connected with the rotary joint, the transfer container is communicated with the separation container through a pipeline, the transfer container is provided with a ventilation connecting part for generating positive pressure or negative pressure in the inside of the transfer container, the pipeline is provided with a first docking part for connecting an initial container, a second docking part for connecting a buffer solution container and a third docking part for connecting a collection container. The application also discloses a disposable cell separation device. In the feeding and discharging process, the material does not directly pass through the positive and negative pressure device and is not repeatedly extruded by the positive and negative pressure device, so that cell damage in the feeding and discharging conveying process is avoided and the cell yield and activity are greatly improved. The disposable cell separation device and the cell separation device have the advantages of avoiding cell damage caused by the suction device and greatly improving the cell yield and activity.