173 results about "Cell isolation" patented technology

The invention belongs to the technical field of cell biology, and particularly discloses a culture medium and a culture method of fish primary liver cells. The culture medium of the fish primary liver cells comprises the following components: 90% of an M199 culture medium, 10% of fetal calf serum, 100 U / mL of penicillin, 0.1 mg / mL of streptomycin, 5 ng / mL of EGF, 10 [mu] U / ml of insulin and 10 mM of dexamethasone. Based on the culture medium, an efficient and stable fish liver cell culture method is developed by combining a standardized anesthesia and disinfection process, a precise dissection and liver sampling method, an optimized tissue digestion and cell separation scheme, an efficient cell purification and adherent culture system, a stable passage technology and the like; a standardized hepatocyte line establishment operation process is defined, and the problems that species binding exists in an existing fish liver cell culture method, and a standardized culture process suitable for multiple fish fingerlings is difficult to form are solved.

The cell separation element has a housing containing a fluid, which is formed by two foil-like housing parts. They are tightly connected to each other along their edges, with at least one integral connection extending along the length of the edge being provided in the edge region. This connection is covered by an elastically deformable cover part provided on the edge of the housing.

An integrated circuit device includes a first cell and a second cell apart from each other in a first lateral direction on a substrate, the first cell and the second cell each including a plurality of gate lines, an inter-cell isolation region between the first cell and the second cell, the inter-cell isolation region extending in a second lateral direction, a power line including portions overlapping a cell boundary of each of the first cell and the second cell in a vertical direction and a portion overlapping the inter-cell isolation region in the vertical direction, a plurality of dummy gate insulation lines, a bridge insulating pattern in contact with an end portion of each of the plurality of dummy gate insulation lines, and a via power rail passing through the bridge insulating pattern, the via power rail being connected to the power line.

The invention relates to a micro-fluidic chip for chemiluminescence imaging detection of single-cell secretions and a use method of the micro-fluidic chip. The micro-fluidic chip is composed of a single cell analysis layer and a glass substrate layer. The single cell analysis layer is prepared from polydimethylsiloxane and is provided with a capture channel, a sample introduction channel and a lantern-shaped microstructure array, the lantern-shaped microstructure comprises a small-size cell capture trap and a large-size chemiluminescence detection chamber, the small-size cell capture trap is connected with the capture channel, and the large-size chemiluminescence detection chamber is connected with the sample introduction channel. After a mixed solution of cells and a detection reagent is introduced into the capture channel, single cell capture is realized on the capture trap due to flow resistance difference; then, introducing air into the capture channel and the sample introduction channel to form a gas-in-liquid microcavity, and carrying out single cell isolation and incubation reaction; finally, the signal switch molecules and the substrate liquid are sequentially injected into the chemiluminescence detection chamber through the sample injection channel, and quantitative detection is conducted on the single-cell secretions according to chemiluminescence imaging signals.

The invention discloses a cell separation device for blood cell detection and a use method thereof, and belongs to the technical field of blood cell detection.The cell separation device is provided with a rotating disc, an adjusting screw rod, a movable adjusting plate, a fixed bottom plate, a supporting bottom frame, a rotating frame and a mounting clamping ring, and the movable adjusting plate moves up and down on the surface of a mounting cylinder to drive the rotating frame to rotate; a mounting clamping ring is pulled to obliquely rotate, so that the placing mechanisms and the test tubes incline around the connecting positions of the placing mechanisms and the test tubes and the supporting bottom frame, and the six placing mechanisms and the test tubes are controlled to incline by the same angle in the centrifugal machine box, so that the test tubes are conveniently inclined by a specific angle according to cell separation requirements, the area of a separation interface is increased, and the thickness of a cell layer is reduced; cell layers are prevented from being mixed, so that the separation effect is improved, the inclination angle of the test tube during rotary centrifugation is conveniently controlled, and the situation that the test tube shakes due to centrifugal force during rotation, and cell sap subjected to centrifugal separation in the test tube is mixed is avoided.

The present invention relates to compositions suitable for cell isolation comprising a polypeptide having protease activity. The invention further relates to polypeptides having protease activity, polynucleotides encoding the polypeptides, the present invention relates to polynucleotides, nucleic acid constructs and expression vectors comprising the polynucleotides, recombinant host cells comprising the nucleic acid constructs or expression vectors, methods of producing the polypeptides, particles comprising the polypeptides, fermentation broth formulations comprising the polypeptides, and methods and uses employing the polypeptides.

Affinity-based and activity-based probes (ABPs) described herein offer transformative resolutions to microbiome function. These ABPs target key metabolic pathways in the gut microbiome. The ABPs contain a binding group, a reactive group, and a. reporter group handle that allows for the addition of a "flexible" reporter group that can be easily swapped to enable multimodal fluorescence and proteomic measurements and isolation of live cells. The binding group, also called an affinity element or biorecognition element, mirrors monomeric and polymeric carbohydrates, sulfated and acetylated carbohydrates, and peptides to afford probe selectivity.

The invention relates to a cell separating element, having a housing (1) in which a fluid is provided and which is formed by two film-like housing parts (2, 3). These are connected to one another in a sealingly fixed manner along their edge (4, 5), wherein at least one integral connection (13) running over the length of the edge (4, 5) is provided in the edge region. Said connection is covered by an elastically deformable cover part (14) which is provided on the edge (4, 5) of the housing.

The invention relates to the technical field of cell separation equipment, in particular to a pipe clamping mechanism and a liquid road faceplate, the pipe clamping mechanism comprises at least one telescopic assembly, a mounting plate and a cover plate, the mounting plate is used for mounting the telescopic assembly, and the extending end of the telescopic assembly can be close to or away from the cover plate to clamp or loosen a hose; the liquid path panel is matched with the pipe clamping mechanism to tightly jack or loosen the hose, so that the hose is convenient to disassemble and assemble, and the flowing condition of a liquid path can be directly observed.

The invention provides a rabbit-source BCR sequence primer group based on high-throughput single cell V (D) J sequencing and application of the rabbit-source BCR sequence primer group. The primer group comprises a specific nested PCR (Polymerase Chain Reaction) primer pair (the sequence is as shown in SEQ ID NO.1-12) aiming at a rabbit BCR heavy chain (IgA / IgE / IgG / IgM) and a light chain (IgK / IgL). Through microfluid single-cell separation, nested PCR amplification and high-throughput sequencing, the naturally paired rabbit BCR full-length V (D) J sequence can be efficiently obtained. According to the rabbit-derived BCR sequence sequencing primer provided by the invention, B cell receptor library information of different tissue sources can be quickly and efficiently obtained. According to the method, paired antibody sequence information can be specifically obtained, B cell response diversity after rabbit immunization vaccine or pathogen infection can be better known, and important data support is provided for design, optimization, effect evaluation and the like of biological products.

The utility model belongs to the field of centrifuge tubes, and particularly relates to a multifunctional centrifuge tube convenient for cell separation and purification, which comprises a tube body and a separation and purification net, the outer side of an opening at the upper end of the tube body is connected with a tube cover through threads, the separation and purification net is positioned on the inner side of the tube body, and the top of the separation and purification net is fixedly connected with a backing ring. A backing ring is arranged in the pipe body and makes contact with the top of the pipe body, a flange is fixedly connected to the outer wall of the pipe body, a clamping pin is slidably connected to the interior of the pipe cover, the clamping pin is connected with the flange in a clamped mode, and a spring is arranged in the pipe cover. The separating and purifying net with the backing ring is arranged in the tube body and used for separating and purifying cells, the functionality of the centrifugal tube is improved, meanwhile, the flange is arranged on the outer side of the tube body, the clamping pin stretching out and drawing back through the spring is arranged in the tube cover, and the clamping pin can be clamped into the flange under the action of the spring, so that the cell separation and purification effect is improved. Therefore, the tube cover has a certain anti-loosening effect, so that the tube cover is not easy to loosen during centrifugation.

To provide: a cell culture vessel that can achieve both detachability and proliferation ability, that is, can facilitate cell detachment without reducing the proliferation ability of cells; and a cell detachment method using the cell culture vessel, provided is a cell detachment method of detaching, through use of a culture vessel having a periodic recess / protrusion pattern on a culture surface, comprising a detachment step of: causing a vibrating unit in which, when an observation image of a cross section perpendicular to the culture surface is obtained, a length Lcp of a contour of the cell on the culture surface side in a measurement region of the observation image and a length Lf of the measurement region in a direction parallel to the culture surface satisfy Lcp>Lf; and transmitting the ultrasonic vibration to the cell, to thereby detach the cell from the culture surface.

A reactor apparatus comprising a reactor vessel 32, media conditioning circuit 34 and a fluid flow controller 36 wherein the fluid flow controller allows the flow of fluid through the reactor vessel to be reversed. The external fermentation broth loop has conditioning modules 50 52 that may alter pH, temperature, gases, or remove waste products. The fluid flow controller may reverse the flow automatically at intermittent periods, and may be a pump or valve. In an alternative configuration, there is a media and cell separation device 42 44 that retains cells inside the reactor vessel, allowing circulation of reaction media between the bioreactor and media loop. The cells may be separated from the media through a filter or membrane, or mechanically separated by gravity or centrifugation. Additionally, there may be several reactor vessels that are connected in a cascading (430, Fig 5) or parallel (330, Fig 5) arrangement. A method of operating the reactor apparatus is also disclosed.

A method of isolating mature adipocytes from a tissue is disclosed. The disclosed method comprises incubating the tissue in a solution comprising an amphiphilic agent and an enzyme. The amphiphilic agent may be Pluronic F-68 and the enzyme may be collagenase. Methods of culturing mature adipocytes comprising culturing mature adipocytes isolated by one of the methods disclosed herein and methods of differentiating fat cells comprising culturing mature adipocytes according to any one of the methods disclosed herein are also disclosed. A kit comprising an amphiphilic agent and an enzyme is disclosed.

The utility model provides a cell separation device and an analysis device based on magnetic nanoparticles, and belongs to the technical field of biomedicine. The separation device comprises a cell and magnetic nanoparticle incubation unit, a cell separation unit and a cell collection unit. Target cells are accurately captured mainly through magnetic nanoparticles with antibodies and are accurately separated out through magnetic field adsorption force of an electromagnetic coil. According to the separation device and the analysis device, the whole multi-channel design in the cell incubation, separation, collection and analysis processes simultaneously realizes the treatment of a plurality of samples and the synchronous separation of different cells, and the accuracy of cell separation is improved through accurate magnetic field control and magnetic nanoparticles. The requirements on high throughput and high precision of cell separation at present are met.

The invention belongs to the technical field of in-vitro cultured calculus bovis, and provides in-vitro cultured calculus bovis and a preparation method thereof. The preparation method comprises the following steps: (1) separating cells; (2) primary culture; (3) cell inoculation and cultivation: adding the serum-free culture medium into the carrier matrix, putting the carrier matrix into a reactor, and performing cell inoculation on the carrier matrix after passage; the inoculated culture comprises a proliferation period; a synthesis period; a crystallization period; and (4) collecting and purifying the calculus bovis, namely stopping culturing after 45 days, and sequentially collecting, cleaning, degreasing, drying and crushing the calculus bovis. The preparation method aims at solving the problems of long culture period, unstable content of effective components, high production cost, large batch difference and the like in the existing in-vitro calculus bovis culture technology by optimizing a culture medium formula, regulating and controlling a culture process and a purification process.

The application provides a back contact cell isolation glue composition and a back contact cell isolation glue. The back contact cell isolation glue composition comprises, in percentage by mass, 60-84% of a resin base material, 10-30% of an active diluent monomer, 0.5-2% of a filler and 2-8% of a photoinitiator; wherein the resin base material comprises silicone acrylate, acrylic monomer and polyurethane modified acrylate; the mass percentage content of amino groups in the polyurethane modified acrylate is 10-30%. The above technical scheme adjusts the proportion of the resin formula, ensures that the isolation glue keeps low yellowing and high light transmittance for a long time, and guarantees the power output and appearance stability of the photovoltaic module. The silicone acrylate makes the thermal decomposition temperature of the isolation glue reach 300-400 DEG C, meets the high-temperature edge passivation process, realizes continuous protection in the whole process of cell manufacturing, and solves the protection problem of the cell piece in the high-temperature process.

The purpose of the present disclosure is to achieve stable, efficient detachment of sample cells from a culture substrate. A cell detachment device according to the present invention detaches cells adhered to a culture surface of a culture substrate from the culture surface and comprises an ultrasonic wave generation unit that generates vibration in the ultrasonic band at the culture substrate, a tapping unit that taps the culture substrate, and a shaking unit that shakes the culture substrate.

The utility model discloses a rapid mononuclear cell separation tube capable of being easily filled with a partition plate, and belongs to the technical field of cell separation, the rapid mononuclear cell separation tube comprises a centrifugal tube and the partition plate provided with three supporting legs, the partition plate is provided with a peripheral through hole, and the side wall of the bottom in the centrifugal tube is provided with a flange surrounding the axis of the centrifugal tube; a supporting cover is arranged at the open end of the centrifugal tube, a supporting rod moving in the axis direction of the centrifugal tube is arranged on the supporting cover, and a tray used for supporting the partition plate and driving the partition plate to move relative to the centrifugal tube in parallel is arranged at the end, located in the centrifugal tube, of the supporting rod. The tray is provided with a protrusion matched with the peripheral through hole, the protrusion is provided with an air hole which is the same as the peripheral through hole and penetrates through the tray, and the device has the advantages that the partition plate can be rapidly and accurately filled under the condition that it is guaranteed that the partition plate is relatively parallel to the inner diameter of the centrifugal tube, the filling efficiency is greatly improved, and the partition plate and the centrifugal tube are firmly fixed.

A cell detachment method for detaching a cell from a substrate by applying vibration to the cell adherent to the substrate includes an incubation step of incubating the cell after bringing a cell detachment liquid containing substantially no proteolytic enzyme into contact with the cell, an information acquisition step of acquiring information relating to the incubated cell, a determination step of determining a time when vibration is applied to the incubated cell in accordance with the information relating to the cell, and a detachment step of applying vibration to the incubated cell.

The invention relates to the technical field of biomedical processing, and particularly provides a cryopreservation method of cord blood mononuclear cells, which comprises six steps of whole blood separation, plasma inactivation, CBMC cell separation, cryopreservation liquid preparation, cell cryopreservation and detection after cryopreservation recovery. The CBMC cell separation comprises three sub-steps; performing cell cryopreservation, namely averagely dividing the separated CBMC into a plurality of parts, filling the CBMC into a cryopreservation tube according to a certain density, putting the cryopreservation tube into a programmed cooling instrument, and performing cryopreservation according to a plurality of programs; and detecting after cryopreservation and resuscitation, wherein the detection comprises three sub-steps of cell viability detection, cell recovery rate detection and cell function detection. The problems that in the cryopreservation process of a traditional method, cell viability is reduced, the number of cells is greatly reduced, cell adhesion and caking are caused, cell functions are abnormal, cell phenotypes are changed after recovery, functional activity is damaged and the like are solved, the utilization rate of the CBMC is increased, and the technical scheme is obviously superior to the traditional cell cryopreservation method; the viability, the recovery rate and the function of the recovered cells are obviously improved.

The application discloses a kind of mitochondrial quality control detection methods, the present application relates to mitochondrial quality control detection technical field, operating steps include preparation sample cell suspension, fluorescent dye labeling, cardiomyocyte separation, establish myocardial cell damaged model and detect mitochondrial membrane potential situation.This mitochondrial quality control detection method can simultaneously carry out multi-parameter, rapid quantitative analysis and sorting, measure fast, measure the multi-parameter characteristics of each cell, while carrying out cell characteristic analysis, can separate out the cell of specified characteristics, so as to further culture, cloning, observation or carry out mitochondrial fragmentation test to specific cell, establish myocardial cell damaged model using doxorubicin, the mitochondrial membrane potential situation of normal myocardial cell and damaged myocardial cell obtained by using JC-1 mitochondrial membrane potential fluorescent probe detection is verified the detection quality of this mitochondrial quality control detection method.

The present invention relates to use of a polypeptide having a sequence identity of at least 70% to SEQ ID NO:1, a mature polypeptide of SEQ ID NO:1, SEQ ID NO:2, or SEQ ID NO:3, wherein the polypeptide has chymopapain activity, in a cell detachment process. The present invention also relates to a method of cell detachment, comprising contacting a cell with a polypeptide having a sequence identity of at least 70% to SEQ ID NO:1, a mature polypeptide of SEQ ID NO:1, SEQ ID NO:2, or SEQ ID NO:3, wherein the polypeptide has chymopapain activity, and wherein the cell is attached to a surface and / or to another cell. The present invention further relates to compositions suitable for cell detachment comprising said polypeptide, polynucleotides encoding said polypeptide, recombinant host cells transformed with said polypeptide, and methods of producing said polypeptide.

This invention discloses a method for preparing and applying nanocluster magnetic beads, comprising the following steps: adding a salt solution containing ferrous and ferric salts to an aqueous solution, dissolving it uniformly, then adding a dispersant to obtain a mixed solution, stirring evenly, and heating; adding ammonia water to the heated mixed solution, stirring the reaction to obtain nanocluster magnetic beads. This invention directly synthesizes nanocluster magnetic beads using an innovative one-step method, eliminating the need for multi-step synthesis and subsequent separation steps, and the synthesized nanocluster magnetic beads can be used for cell separation and MRI imaging.

The invention discloses a blood cell separation magnet device and method, and belongs to the technical field of cell separation. The magnet device comprises a plurality of pole heads which are symmetrically arranged, and runway coils are arranged outside the pole heads; the central axis position of a gap defined by the runway coils is a working area, the working area is a uniform magnetic field capturing area, and magnetized target cells in blood cells are stably separated by using a uniform magnetic field; the width of the working area ranges from 8 mm to 15 mm, and the length of the working area ranges from 200 mm to 500 mm. Through cooperation of the runway coil and the pole head, a linear or planar working area which is small in space body, high in strength, large in gradient and uniform in magnetic field distribution is formed, and compared with an existing point-shaped or small-area high gradient, magnetized cells flowing through a sample in the area can be captured more efficiently and more thoroughly, and the recovery rate and the purity are remarkably improved.

Devices and methods suitable for cell separation. The devices herein include non-random voids interconnected through non-random pores and / or non-random solid geometrical structures optionally connected through solid non-random interconnecting elements. Such devices are preferably suitable for affinity-based cell isolation techniques which rely upon binding interactions.

In one aspect, the disclosure relates to a method for neuromodulation in a target, wherein the target can be an isolated plurality of cells, an isolated organ, or an animal subject In an aspect, the method includes at least the step of delivering X-ray radiation to one or more neurons in the target, wherein the one or more neurons express dTRPA1(A)10b, and wherein the X-ray radiation generates H2O2 via radiolysis of water. In a further aspect, the H2O2 can increase intracellular calcium ions which can, in turn, stimulate the release of neurotransmitters. Also disclosed are treatments of diseases, disorders, and symptoms thereof using the disclosed method.

The present application relates to the technical field of CAR-T cell treatment, and particularly relates to a method for preparing universal CAR-T cells and application thereof. The VH domain and VL domain of the beta2M binding molecule provided in the present application contain CDR sequences, and can specifically bind to beta2M. The binding molecule is added to CAR-T cells, and the binding molecule can specifically bind to CAR-T cells without beta2M gene knockout, so as to separate CAR-T cells with beta2M gene knockout from CAR-T cells without beta2M gene knockout, and realize purification of universal CAR-T cells.

The invention relates to a system, comprising: a) a sample processing unit, comprising an input port and an output port coupled to a rotating container having at least one sample chamber, the sample processing unit configured provide a first processing step to a sample or to rotate the container so as to apply a centrifugal force to a sample deposited in the chamber and separate at least a first component and a second component of the deposited sample; and b) a sample separation unit coupled to the output port of the sample processing unit, the cell separation unit comprising separation column holder, a pump and a plurality of valves configured to at least partially control fluid flow through a fluid circuitry and a separation column positioned in the holder, the separation column configured to separate labeled and unlabeled components of sample flowed through the column.

1. Name of the design product: bracelet (blood cell separation sheet). 2. Use of the design product: the design product is a bracelet worn on the wrist, which activates health conditions naturally through physical therapy in a non-drug and non-chemical way. 3. Design points of the design product: in the combination of shape and pattern. 4. Picture or photo best indicating the design points: perspective view 1.