12 results about "Mixed Cellular Population" patented technology

Methods for characterizing genome editing, clonal expansion and associated reagents for use in such methods are disclosed herein. Some embodiments of the technology are directed to characterizing a population of cells following an engineered genomic editing event, that includes in some embodiments characterizing genomic alterations occurring at both intended and unintended genomic loci within the genome of the populations of cells. Other embodiments are directed to utilizing Duplex Sequencing for assessing a clonal selection in mixed cell populations and / or cell populations following a genomic editing event. Further examples of the present technology are directed to methods for detecting and assessing clonal expansion of cells following a genomic editing event.

The present invention relates to a cell population of spiral ganglion neural progenitors (SGNPs) and methods how to isolate these cells from an in-vitro mixed cell population. The cell population expresses one or more markers, wherein the one or more marker is CXCR4, SUSD2, LPAR3, EPHA5, CHRNA3, REEP1, NPFFR2, SORCS3, NFASC, GABRB3, KCNB2, GRM8, DLL3, and / or KCNH8.

The invention provides a microalgae cell swelling inertia screening and impedance detection integrated micro-fluidic device, and relates to the technical field of microalgae cell screening and detection. The microalgae cell swelling inertia screening and impedance detection integrated micro-fluidic device comprises a signal processing unit, the substrate is ITO (Indium Tin Oxide) conductive glass; the main micro-channel is of a micro-channel structure which is formed in a polydimethylsiloxane (PDMS) layer and is bonded on the substrate. According to the invention, the passive sorting module based on the expansion and contraction inertia effect and the differential impedance detection module are integrated in the single micro-fluidic chip, so that the full-process automation of'sample introduction-sorting-detection-output 'is realized. Sample loss, cross contamination and activity damage are avoided, interference of mixed cell populations on subsequent impedance signals is effectively eliminated through pre-sorting, and the accuracy of target microalgae detection is remarkably improved.

The present disclosure provides a method of performing a functional assay on human spheroids, e.g., three-dimensional human cell spheroids using, in one embodiment, a fluorometric imaging plate reader.

Several embodiments disclosed herein relate to methods and processes for the co-expansion of multiple types of immune cells, in order to generate a mixed cell population. Some embodiments relate to the use of various stimuli specific to the various subpopulations to achieve expansion of those subpopulations at a particular time in a culturing process in order to generate an expanded population of immune cells having a desired ratio of the various subpopulations. In several embodiments, such mixed cell populations exhibit desirable characteristics, such as cytotoxic effects against tumor cells that enhance the efficacy of cancer immunotherapy.

Methods for characterizing genome editing, clonal expansion and associated reagents for use in such methods are disclosed herein. Some embodiments of the technology are directed to characterizing a population of cells following an engineered genomic editing event, that includes in some embodiments characterizing genomic alterations occurring at both intended and unintended genomic loci within the genome of the populations of cells. Other embodiments are directed to utilizing Duplex Sequencing for assessing a clonal selection in mixed cell populations and / or cell populations following a genomic editing event. Further examples of the present technology are directed to methods for detecting and assessing clonal expansion of cells following a genomic editing event.

Materials and methods for labeling and isolating particular cell types from mixed cell populations are provided herein. Also provided herein are methods for generating data representing a synthetic genetic sequence configured for labeling at least one cell type by causing expression of a marker in the at least one cell type.

Provided herein are methods of enriching for photoreceptors and photoreceptor precursor cells from mixed cell populations. Such enriched populations of photoreceptors and photoreceptor precursor cells can be used as cell therapies for the treatment of retinal disorders.

The disclosure includes a method for producing an expanded population of engineered γδ T cells, comprising: (a) culturing an isolated mixed cell population comprising γδ T cells with at least one T cell mitogen to produce an activated γδ T cell population; (b) depleting αβ T cells in the activated γδ T cell population to produce an enriched γδ T cell population; (c) engineering the enriched γδ T cell population to express at least one antigen recognition moiety to produce an engineered γδ T cell population, the engineering step comprising stably integrating at least one nucleic acid construct encoding said at least one antigen recognition moiety in culture conditions comprising IL-4, IL- 13, and IL-21; and (d) culturing the engineered γδ T cell population in the presence of at least one cytokine to provide the expanded population of engineered γδ T cells.

The invention particularly discloses a preparation method and application of high-purity neural stem cells. The method comprises the following steps: firstly, acquiring haploid neural stem cells, injecting the haploid neural stem cells into blastocysts, and culturing to form chimera embryos; separating nervous tissues from the embryos and dissociating the nervous tissues into mixed cell populations; finally, the target cells are efficiently enriched through a magnetic separation technology by utilizing the functionalized magnetic nanoparticles with the surfaces modified with the neural stem cell specific antibodies. According to the method, the genetic homogeneity advantage of a haploid technology, the in-vivo bionic development environment of chimera embryos and the label-free and high-precision purification capacity of magnetic separation are creatively fused, and the key technical bottlenecks of low purity, high heterogeneity and complex genetic background in traditional neural stem cell preparation are solved. The invention also provides the high-purity neural stem cell population prepared by the method, the special functionalized magnetic nanoparticles and application of the functionalized magnetic nanoparticles. The obtained cell population is high in purity and stable in function, and has important application value in the fields of disease modeling, drug screening, cell therapy and the like.

Aspects of the invention described herein, concern approaches to make genetically modified T-cells comprising a chimeric antigen receptor for human therapy. In some alternatives, the methods utilize a selection and / or isolation of CD4+ and / or CD8+ T-cells from a mixed T-cell population, such as, peripheral blood or apheresis derived mononuclear cells. Once selected / isolated, the CD4+ and / or CD8+ T-cells are then activated, genetically modified, and propagated, preferably, in separate or isolated cultures in the presence of one or more cytokines, which support survival, engraftment and / or proliferation of the cells, as well as, preferably promoting or inducing the retention of cell surface receptors, such as CD62L, CD28, and / or CD27. Included herein are also methods of treatment, inhibition, amelioration, or elimination of a cancer by administering to a subject in need thereof, one or more types of the genetically engineered T-cells or compositions that comprise the genetically engineered T-cell prepared as described herein.