472 results about "Red blood corpuscle" patented technology

The invention discloses a reticulocyte recognition and grading system based on a blood smear, and particularly relates to the field of medical cell morphology examination, and the system comprises an image acquisition module, a staining normalization module, an instance segmentation module, an erythrocyte classification module, a reticulocyte grading module and a statistics output module. The image acquisition module adopts an automatic microscope platform to continuously scan the blood smear under a 100-time visual field, acquires a high-quality bright field channel image through an automatic focusing technology, and monitors image definition, contrast and illumination uniformity; the statistical output module is used for calculating the proportion and bluish violet intensity statistics of reticulocytes of each level, and generating a visual quality control result and a diagnostic report; according to the invention, full-automatic processing from samples to reports is realized, interpretability and high classification precision are both considered, subjective difference and omission ratio of manual microscopic examination are significantly reduced, and efficiency and consistency of clinical detection are improved.

The invention provides a red blood cell test time domain adaptation detection method based on an average teacher model, which belongs to the field of deep learning and comprises the steps of pre-training, data enhancement, random recovery, loss calculation and update of a teacher model and a student model. First, a benchmark detection model is pre-trained on source domain data to obtain initial features. In the test stage, target domain data is input into the teacher model and the student model after being subjected to weak enhancement and strong enhancement respectively. A pseudo label is generated after the teacher model is input, so that the influence of error accumulation in the continuous adaptation process is reduced; the student model is optimized through the cross entropy loss of the prediction result of the teacher model, and the teacher model is updated through an index moving average method. In addition, a random recovery method is adopted, knowledge is explicitly recovered from a source domain pre-training model to a student model, and the problem of disastrous forgetting is effectively relieved. And the final model can keep good performance for target domain data during testing.

The invention belongs to the technical field of biological medicine, and particularly relates to application of chlorbuconazole or salt thereof in preventing, relieving or treating diseases related to HIF-2alpha. In-vitro experiments prove that the chlorbuconazole enhances the transcriptional activity of HIF-2alpha so as to promote the expression of downstream genes regulated and controlled by the HIF-2alpha; it is proved that chlorburazol has the effects of increasing erythropoiesis and relieving renal injury on an aristolochic acid-induced zebra fish chronic kidney disease model, a cisplatin-caused acute kidney injury and renal anemia model and a unilateral ureteral obstruction model. Meanwhile, the traditional Chinese medicine composition belongs to new use of old medicines, so that the medicine research and development cost and the treatment cost are saved, and the traditional Chinese medicine composition has obvious economic benefits and clinical application values.

The invention discloses a diluent for whole blood lateral immunochromatography detection as well as a preparation method and a detection method thereof, and belongs to the technical field of immunochromatography detection. The diluent solves the technical problems of unsmooth chromatography, background interference, poor stability of detection results and the like caused by red blood cells during direct detection of whole blood samples. The diluent comprises a buffer system, an osmotic pressure regulator glycine, a nonionic surfactant polysorbate, an erythrocyte aggregation inducer polyvinylpyrrolidone, a dynamic viscosity regulator trehalose, a blocking protein, a stabilizer combination L-methionine and L-histidine, and a composite formula of water. Red blood cells are effectively induced to gather and intercept on the sample pad through the synergistic effect of all the components, and meanwhile the stability of the chromatography process and the activity of the detection reagent are maintained. The diluent is mainly used for lateral immunochromatography quantitative detection of markers such as pro-enkephalin, C-reactive protein, cardiac troponin I or N-terminal pro-brain natriuretic peptide in a whole blood sample.

The invention discloses an intelligent pulmonary tuberculosis detection method and system based on images and clinical data, and belongs to the field of medicines.The method comprises the steps that S1, high-resolution chest X-ray images and clinical data such as serum C-reactive protein concentration, erythrocyte sedimentation value, erythrocyte distribution width and body temperature are collected; s2, performing non-linear contrast enhancement on the image, and extracting lung gate texture, lung field transparency and cavity edge features; s3, fusing the image and the clinical features through a graph attention mechanism, and generating a high-dimensional fusion vector; s4, risk grade discrimination is carried out through a multi-scale residual mapping network, and five-grade labels are output; and S5, optimizing the model by utilizing the pathology definite diagnosis sample in combination with the cross entropy and the center loss. The method has the beneficial effects that accurate grading evaluation of the pulmonary tuberculosis risk is realized, and the accuracy of early screening and the clinical interpretability of the model are improved.

The invention relates to the technical field of biological detection, and provides a kit and a method for rapidly detecting a platelet antibody by utilizing a phytolectin solid-phase adsorption method. The detection kit provided by the invention comprises a reaction plate which is coated with phytolectin and adsorbs platelets. According to the method, the platelet of multiple persons is adsorbed by utilizing the non-specific adsorption capacity of the phytolectin, and the adsorbed platelet is directly freeze-dried to the bottom of a microwell plate. During use, a detection result can be obtained only by adding detected plasma / serum into the reaction plate for incubation and then adding an anti-human globule reagent and indicating red blood cells, so that intermediate links of detection are reduced, and standardization and automation of detection are facilitated.

The invention discloses application of capsaicin to resistance of grass carp to hypoxia stress, and belongs to the technical field of agricultural cultivation. Aiming at the problems of red blood cell function damage, liver and gill tissue damage and the like of grass carps under hypoxia stress, a trace amount of capsaicin is added into the grass carp feed, so that the feed formula is optimized, the reduction of red blood cell quantity and brittleness increase of grass carps in the later growth period caused by hypoxia stress are relieved, liver damage is relieved, and the growth period of grass carps is prolonged. The death rate of the grass carp in a low-oxygen environment is reduced, the culture risk is reduced, and the method has the characteristics of being green, safe, low in cost and remarkable in economic benefit and is suitable for large-scale grass carp culture industry.

The invention relates to the technical field of bioengineering, in particular to an SOD (superoxide dismutase) impurity removal and purification process which comprises the following steps: extracting SOD enzyme from fresh animal blood, separating high-purity red blood cells through gradient centrifugation, preparing a crude extract by combining hypotonic lysis and freeze thawing strengthening, then selectively precipitating hemoglobin and other heat-sensitive impurity proteins by adopting a gradient temperature rise thermal denaturation method, and purifying to obtain the SOD impurity removal and purification process. The method comprises the following steps: extracting SOD, removing pigments and lipid impurities by using a mixed adsorbent, further enriching SOD by using ammonium sulfate salting-out, and finally obtaining high-purity SOD powder through vacuum freeze drying by combining ion exchange chromatography and ultrafiltration concentration technologies. The method has the advantages of high efficiency, rapidness and low cost, impurities can be effectively removed through a combined process of hypotonic / freeze-thaw cracking, hemoglobin removal through thermal denaturation, ammonium sulfate salting-out, ion exchange chromatography and ultrafiltration concentration, the purity and the recovery rate of the SOD enzyme are remarkably improved, the whole purification process is carried out at 4 DEG C, and the method is suitable for industrial production. The influence of high temperature on the activity of the SOD enzyme is avoided.

The invention relates to the technical field of thrombus mining robots, in particular to a micron robot with an asymmetric cilia structure, a preparation method and application. The micro-robot is of an asymmetric composite structure and is composed of a smooth hemisphere and a cilia-shaped structure, sandwich type layered arrangement of a PLGE cilia-shaped nano structure, an MNPs interlayer and a PS sphere core is formed on the surface and the interior of the micro-robot from outside to inside, and cooperative regulation and control of morphology and functions are achieved by regulating and controlling the material proportion of PS and PLGE. The thrombus mining robot shows an efficient thrombolysis effect in a plurality of in-vitro and in-vivo models, and rapid fibrinolysis, deep thrombus penetration and efficient mining of single red blood cells are realized, so that the limitation of a traditional thrombolysis agent is overcome. In addition, the thrombus digging robot has excellent biocompatibility and biological safety, has no obvious cytotoxicity or damage to main organs, and supports the safety of the thrombus digging robot in clinical application.

Separation of the cellular components of whole blood, or other biological fluid, from plasma or serum can be achieved for assay analysis. A device for facilitating separation can include, for example, a capillary tube that accurately draws target blood volume, a pad that chemically interacts with red-blood cells, such that the red blood cells become chemically and / or physically trapped within pad material, a mechanism for plasma recovery from the pad upon diffusion or active mixing, and a dropper tip that facilitates dispensing the mixture onto a test device. The treatment of the cellular components can be performed prior to contact with a buffer solution, so release of the cellular components into the buffer solution is reduced or prevented. Additional filtration can be provided to filter any remaining cellular components in the mixture.

The invention provides a multi-channel viscoelastic micro-fluidic chip, a manufacturing method and a method for separating MCF-7 cells. The multi-channel viscoelastic micro-fluidic chip comprises a first multi-stage shunting module, a pipette, a second multi-stage shunting module, a single-stage viscoelastic micro-fluidic sub-module and a third multi-stage shunting module, the first multi-stage shunting module is connected with one inlet of the single-stage viscoelastic micro-fluidic sub-module through a pipette; the second multi-stage shunting module is connected with the other inlet of the single-stage viscoelastic micro-fluidic sub-module; and the outlet of the single-stage viscoelastic micro-fluidic sub-module is connected with the third multi-stage shunting module. According to the method, the PEO solution is used as sheath fluid, and the action of elastic force is introduced to cells, so that CTC can be directly separated from undiluted human blood in an ultrahigh-flux manner, and the limitation that erythrocytes need to be split and a blood sample needs to be diluted by dozens of times before cell separation in traditional inertial microfluidics is broken through; and the complexity of operation and the risk of sample pollution are reduced.

A system and a method for measuring deformability of red blood cells in microfluidic channels are disclosed. The system comprises one or more devices having one or more microfluidic channels with at least one cross-sectional dimension and configured to allow deformation of red blood cells of a blood sample to flow through the one or more microfluidic channels. Further, at least one imager is configured to generate a sequence of digital holography images or videos of the red blood cells of the blood sample transiting through the one or more microfluidic channels. Further, the system includes at least one processor that is operationally coupled to the at least one imager. The at least one processor is configured to analyze the generated sequence of digital holography images or videos to quantify and characterize deformability of the red blood cells within the one or more microfluidic channels.

The invention belongs to the technical field of biology, and particularly discloses a system for detecting HIV RNA, a fluorescent biosensor and preparation and application of the fluorescent biosensor. The system comprises red blood cells, a first report chain, a second report chain, crRNA, Cas12a and a DNA hairpin probe H1. On the basis of the system, a fluid membrane-red blood cell confinement Cas12a can be constructed, and a simple, sensitive and specific fluorescence detection sensing strategy of virus RNA such as HIV RNA is developed, so that the problems of low reaction efficiency, complex reaction steps and the like of an HIV RNA detection method based on CRISPR-Cas12a in the prior art are solved.

The invention discloses a composition for preventing and treating plateau erythrocythemia and application of the composition, and relates to the technical field of pharmaceutical composition preparation. The composition is prepared from PT2385 and salidroside. The combination of PT2385 and salidroside can effectively inhibit hyperplasia of red blood cells of HAPC rats, reduce hemoglobin and relieve blood flow stasis and microcirculation disturbance of body tissues and organs caused by hypoxia, so that hypoxia phenotypic characteristics of the HAPC rats are improved. In addition, free radicals can be effectively removed, oxidative stress, peroxidation reaction and inflammatory reaction induced by hypoxia are inhibited, the mitochondrial function is protected, and the dysfunction of HAPC rat tissue and organ injury is improved. The problem that in the prior art, an effective medicine for preventing and treating plateau erythrocythemia is lacked is solved.

A system and a method for measuring adhesion of blood cells in microfluidic channels are disclosed. The system includes a device having one or more microfluidic channels configured to adhere a plurality of diseased red blood cells (RBCs) of a blood sample on an interior surface of the one or more microfluidic channels. Further, the system includes at least one imager configured to generate one or more digital holography images or videos of the plurality of diseased RBCs adhered to the interior surface of the one or more microfluidic channels. Further, at least one processor is operationally coupled to the at least one imager and configured to receive the one or more digital holography images or videos and analyze the generated one or more digital holography images or videos to quantify adhesion of the plurality of diseased RBCs to the interior surface of the one or more microfluidic channels.

The utility model discloses a red blood cell osmotic brittleness colorimetric experiment analyzer which comprises a working stand, a centrifugal assembly used for separating red blood cells is fixedly arranged on the upper surface of the working stand, a solution assembly used for placing solutions with different concentrations is fixedly arranged on the upper surface of the working stand, the solution assembly comprises a solution box, and a mixing assembly comprises a limiting disc. The mounting block is fixedly mounted on the upper surface of the sliding block, one side of the drawing block and the telescopic end of the telescopic motor are mounted through a bolt, the drawing end of the negative pressure suction pipe and the drawing block are fixedly mounted, the negative pressure suction pipe is arranged on the upper surface of the reagent bottle in a sliding mode, and the negative pressure suction pipe extracts solutions in the reagent bottle and puts the solutions in the mixing test tube. The negative pressure suction tube is driven by the telescopic motor to move between the reagent bottle and the mixing test tube, and a required amount of solution is sucked under the action of negative pressure; and then the solution is moved above the mixing test tube, and the solution is released into the test tube, so that powerful support is provided for the erythrocyte osmotic brittleness experiment, and errors caused by manual operation are reduced.

The application discloses a use of Ejiao Qianggu oral liquid in regulating immunity, and particularly relates to an improving effect of the Ejiao Qianggu oral liquid on an immune system, which is realized by the following aspects. On one hand, the Ejiao Qianggu oral liquid promotes proliferation and transformation (mainly early T lymphocytes and part of B lymphocytes) and active functions of spleen lymphocytes; on the other hand, the Ejiao Qianggu oral liquid improves phagocytosis of macrophages. The Ejiao Qianggu oral liquid has the following characteristics: firstly, the Ejiao Qianggu oral liquid acts on early lymphocyte maturation, and has no significant effect on proliferation of peripheral blood lymphocytes; secondly, the Ejiao Qianggu oral liquid can significantly act on immune target organs, thymus and spleen tissues, and does not excessively stimulate the spleen, unlike positive drugs such as colony stimulating factor which can cause significant edema of the spleen; and thirdly, the Ejiao Qianggu oral liquid has a significant recovery effect on body weight, white blood cells, lymphocytes, red blood cells and hemoglobin in blood of patients with low immunity.

Therapeutic compounds for red blood cell-mediated delivery of an active pharmaceutical ingredient to a target cell are described. The therapeutic compounds are configured to bind CD47 on the surface of a red blood cell and to be subsequently transferred to CD47 on the surface of the target cell, the therapeutic compound ultimately being internalized by the target cell via endocytosis. The target cell may be a cancer cell, a virus-infected cell, or a fibrotic cell.

The present application relates to the technical field of vaccine production, in particular to a preparation method of a Sapindus saponin veterinary vaccine adjuvant, comprising: determining the limit concentration of Sapindus saponin with a hemolysis rate lower than 5% and preparing the vaccine adjuvant; determining the limit concentration of Sapindus saponin with a hemolysis rate lower than 5% against red blood cells by spectrophotometry; preparing the vaccine adjuvant by dialysis; and the vaccine adjuvant contains soybean lecithin and cholesterol in a weight ratio of 1:1. The present application first determines the safe use concentration of Sapindus saponin through a standard hemolysis experiment, ensures that the hemolysis rate of the prepared adjuvant meets the safety requirements, and avoids adverse reactions after injection; at the same time, the present application uses soybean lecithin and cholesterol as raw materials, and prepares the nano-liposome adjuvant embedding Sapindus saponin by dialysis, which does not require complex process operation, has low preparation cost, and has safety and practicability, and can effectively assist the vaccine to induce the body to produce stronger immune response and improve the protection effect of the vaccine.

This invention relates to a body fluid-based detection device for chronic diseases, which belongs to the field of medical devices. The device includes a body fluid detection fluid channel, a detection probe, a data processing module, and a data display module. The detection probe is disposed within the body fluid detection fluid channel, and the detection probe is connected to the data processing module, which is connected to the data display module. The body fluid detection fluid channels include: a body fluid ion detection channel, a body fluid pH detection channel, a body fluid hydrodynamic characteristic detection channel, a channel for detecting the content of soluble gases such as oxygen, a channel for detecting the physical characteristics of white blood cells and tumor cells, a channel for detecting the electrical characteristics of white blood cells and tumor cells, a channel for detecting the physical characteristics of red blood cells, a channel for detecting the electrical characteristics of red blood cells, a channel for detecting the electrical characteristics of separated body fluids, and a channel for detecting anticoagulation and fibrinolysis functions. This invention enables highly sensitive, non-invasive testing, providing a powerful tool for the early diagnosis of chronic diseases.

The present disclosure relates to systems, devices, and methods for measuring analyte levels. A glycosylated hemoglobin level measurement system includes a sample test cartridge having microchannels that can passively transfer and compress cells. The system may execute one or more calibration or normalization routines on the sensor data to generate calibrated or normalized data. The calibrated or normalized data may be used to determine one or more analyte levels.

The invention discloses a red blood cell room-temperature preservation solution as well as a preparation method and application thereof, and belongs to the technical field of biological medicines. The erythrocyte preservative comprises the following components in parts by weight: 1-200 parts of an anticoagulant, 1-200 parts of a blood cell stabilizer, 1-100 parts of a metabolic inhibitor, 1-100 parts of an electrolyte, 1-150 parts of an antioxidant and 350-1000 parts of deionized water, and the pH value of the erythrocyte preservative is 4.6-7.0. All the components in the preservative are mutually matched and have a synergistic effect, and the integrity of erythrocyte membranes can be kept through synergistic protection of the multiple components; the electrolyte equilibrium osmotic pressure is maintained, the antioxidant scavenges free radicals, and hemolysis is reduced; the preservation time is prolonged, and the cell morphology and function are good; the dependence on a cold chain is reduced, and the demand on deep hypothermia equipment is reduced.

The present invention provides a device for classifying and counting white blood cells, comprising: an upper magnet and a lower magnet, with a sample channel disposed between the upper and lower magnets; a sample inlet and a buffer inlet disposed at one end of the sample channel; a white blood cell output port and a red blood cell output port disposed at the other end of the sample channel, the white blood cell output port being connected to the input end of a white blood cell shunt tube, the output end of the white blood cell shunt tube being connected to a sorting chamber; and a plurality of sensors mounted at the output end of the sorting chamber for classifying and counting purified samples. The device provided by the present invention is compact, portable, and provides accurate measurement results. It also avoids the drawbacks of traditional methods, such as the need for high-precision camera acquisition components, the reduced reliability due to compromised image quality, the generation of significant noise due to the need to lyse red blood cells prior to detection, and the resulting lack of data support for diagnosis of related diseases due to red blood cell lysis.

The invention discloses a fetal nucleated red blood cell sorting device and method based on inertia focusing microfluidics, and belongs to the field of cell screening. A fetal nucleated red blood cell sorting device based on inertia focusing microfluidics comprises an air pump, an anti-backflow device, a sample injection valve, a sample injection pipe, a micro-fluidic chip, a waste liquid pipe and a sorting pipe, the output end of the air pump is connected with the input end of the anti-backflow device through a hose, the output end of the anti-backflow device is connected with the input end of the sample injection valve through a hose, and the output end of the sample injection valve is connected with the output end of the micro-fluidic chip. The sample injection valve is in threaded connection with the sample injection pipe, and the output end of the sample injection valve is connected with a sample injection port of the micro-fluidic chip through a hose. According to the method, high-flux sorting of the fetal nucleated red blood cells (fNRBCs) in the peripheral blood of the pregnant woman is realized, and the recovery rate is high; meanwhile, the sorting process is mild, the cell viability and functional integrity of the fNRBCs are not influenced, and the accuracy and reliability of detection results such as subsequent antenatal diagnosis are ensured.

The present invention relates to a kit for determining folic acid and its metabolites in red blood cells by high performance liquid chromatography tandem mass spectrometry. The kit solves the problem of quantifying red blood cells by detecting the content of folic acid and its metabolites in red blood cells. The kit has a simple pretreatment process for the entire sample to be tested, high sensitivity, small sample volume, and strong specificity. Five types of folic acid and their metabolites in red blood cells can be simultaneously detected within 7.0 minutes. The kit can be used for clinical diagnosis of the five types of folic acid and their metabolites in red blood cells and health assessment.

The invention discloses a composition for improving adverse effects of chemotherapy as well as a preparation method, application and a product of the composition, and particularly relates to the technical field of medicines. The composition comprises the following raw materials: tortoise, soft-shelled turtle, antler, sea cucumber, fructus amomi, pericarpium citri reticulatae and hawthorn. Alkaline protease and flavourzyme are adopted for enzymolysis, and the obtained composition can remarkably reduce the tumor volume of tumor-bearing animals, increase the bone marrow DNA content of chemotherapy animals and remarkably increase the number of white blood cells and the number of red blood cells in peripheral blood of the chemotherapy animals. The composition disclosed by the invention is used for preparing related products for improving the adverse effects of chemotherapy, and the prepared products are easy to absorb and have a remarkable improvement effect on the adverse effects of chemotherapy.

The invention provides a peripheral blood cell morphological analysis system and a heart failure degree judgment method, and the method comprises the steps: taking the peripheral blood cell morphological analysis system as the basis, obtaining the fluorescence signal, intensity, phase, bright field and dark field images of red blood cells, building the relation between the heart failure degree and red multi-dimensional features, and determining the heart failure degree of the red blood cells; and constructing, training and optimizing an erythrocyte morphological feature extraction network model based on machine learning. On the basis, a red blood cell characteristic image feature extraction network model with a fluorescence label is adopted to analyze a red blood cell image obtained by detecting a peripheral blood sample by a multi-parameter flow cytometry imaging system, the correlation between the characteristics of red blood cells in the peripheral blood sample and the heart failure degree is analyzed, and the heart failure degree is judged quickly and precisely.

The present application relates to the application of Bifidobacterium longum subsp.longum BL21 in the preparation of a preparation for improving hematopoietic dysfunction, wherein the Bifidobacterium longum subsp.longum BL21 is a Bifidobacterium longum subsp.longum BL21 strain with a preservation number of CGMCC No.10452.The present application develops a new microbial strategy for improving hematopoietic dysfunction, and uses the Bifidobacterium longum subsp.longum BL21 in the preparation of a preparation for improving hematopoietic dysfunction.It is found that the Bifidobacterium longum subsp.longum BL21 can increase the number of peripheral blood cells (red blood cells and white blood cells), the content of platelets or the content of hemoglobin, reverse the weight loss caused by hematopoietic dysfunction, and improve the serum inflammatory response and the spleen inflammatory response caused by hematopoietic dysfunction.The present application also provides a new idea for the wide application of the Bifidobacterium longum subsp.longum BL21.

This invention belongs to the biomedical field and discloses a deep learning-based microscopic hyperspectral detection method for abnormal red blood cells. It constructs a Fusion-SSD abnormal red blood cell detection model based on fused data, consisting of a spectral data extraction module, a spectral feature extraction module, an improved SSD detection model, and a feature fusion module. This invention analyzes the backbone feature extraction network structure of the improved SSD detection model and, combined with the image characteristics of abnormal red blood cells, adds an effective feature layer to the backbone feature extraction network. Appropriate effective feature layers are then selected for top-down fusion, improving the detection capability for abnormal red blood cells. Compared with existing methods for detecting abnormal red blood cells, the improved SSD detection model enables rapid, non-destructive, and automated detection of abnormal red blood cells, effectively avoiding inter-class confusion caused by single-type data.

The embodiment of the invention relates to a sample analyzer and a sample analysis method. The sample analysis method comprises the following steps: sucking a to-be-detected blood sample; mixing at least one part of the blood sample to be tested, the hemolytic agent, the first dye and the second dye to prepare a test sample; in a single test, each particle in a measurement sample passes through an optical detection zone irradiated by excitation light one by one to acquire optical information including scattered light information, first fluorescence information, and second fluorescence information, the exciting light comprises light of a first wavelength capable of exciting the first dye and light of a second wavelength capable of exciting the second dye, and the second wavelength is larger than the first wavelength; and obtaining a white blood cell four-classification result and a counting result of a nucleated red blood cell group of the test sample based on the at least one kind of scattered light information, the first fluorescence information and the second fluorescence information. Therefore, the leukocyte classification result and the nucleated red blood cell counting result can be obtained at the same time in one test.