49 results about "Red blood corpuscle" patented technology

The present application relates to the application of Bifidobacterium longum subsp.longum BL21 in the preparation of a preparation for improving hematopoietic dysfunction, wherein the Bifidobacterium longum subsp.longum BL21 is a Bifidobacterium longum subsp.longum BL21 strain with a preservation number of CGMCC No.10452.The present application develops a new microbial strategy for improving hematopoietic dysfunction, and uses the Bifidobacterium longum subsp.longum BL21 in the preparation of a preparation for improving hematopoietic dysfunction.It is found that the Bifidobacterium longum subsp.longum BL21 can increase the number of peripheral blood cells (red blood cells and white blood cells), the content of platelets or the content of hemoglobin, reverse the weight loss caused by hematopoietic dysfunction, and improve the serum inflammatory response and the spleen inflammatory response caused by hematopoietic dysfunction.The present application also provides a new idea for the wide application of the Bifidobacterium longum subsp.longum BL21.

This invention belongs to the biomedical field and discloses a deep learning-based microscopic hyperspectral detection method for abnormal red blood cells. It constructs a Fusion-SSD abnormal red blood cell detection model based on fused data, consisting of a spectral data extraction module, a spectral feature extraction module, an improved SSD detection model, and a feature fusion module. This invention analyzes the backbone feature extraction network structure of the improved SSD detection model and, combined with the image characteristics of abnormal red blood cells, adds an effective feature layer to the backbone feature extraction network. Appropriate effective feature layers are then selected for top-down fusion, improving the detection capability for abnormal red blood cells. Compared with existing methods for detecting abnormal red blood cells, the improved SSD detection model enables rapid, non-destructive, and automated detection of abnormal red blood cells, effectively avoiding inter-class confusion caused by single-type data.

State of art techniques for measurement of blood viscosity, RBC concentration and the like are in vitro methods. Embodiments of the present disclosure provide a method and system for in vivo non-invasive measurement of blood parameters using Laser Enabled Swept Frequency Acoustic Interferometry (LE-SFAI) principle. A Continuous Wave Laser diode based Photo Acoustic (CWPA) signal in frequency domain generated from laser excitation incident on a biological tissue of a subject carrying blood vessel is acquired by the ultrasound sensor and converted to a windowed time domain CWPA signal limited to two peaks. The signal characteristics such as risetime, amplitude, and time difference between first peak and the second peak are obtained to derive blood viscosity, Red Blood Corpuscles (RBC) concentration, and blood vessel diameter.

The application provides a method for separating nucleic acid binding proteins in solid tissue cells, and the method comprises the following steps: step S1, preparing solid tissue into a cell suspension 1; step S2, cross-linking the cell suspension 1 by using a cross-linking agent to form a cell suspension 2 with nucleic acid-NABPs complexes; step S3, lysing red blood cells in the cell suspension 2 of step S2 to remove high-abundance protein interference; step S4, after the cells treated in step S3 are lysed by using an organic reagent, the nucleic acid-NABPs complexes in the solid tissue cells are selectively enriched by using affinity chromatography; and step S5, after the nucleic acid-NABPs complexes obtained in step S4 are de-cross-linked, nucleic acid binding proteins (NABPs) in the solid tissue cells are obtained.

The present invention relates to a colorimetric test sheet equipped with multi-color reference points, an image correction method, and a system for overcoming non-linear image distortion of a smartphone camera. To precisely correct the inherent color distortion of smartphones (ISP, AWB, etc.) that is impossible with basic three-color markers, the present invention places a plurality of multi-color reference color patches around a QR code. In addition, to prevent handling contamination such as fingerprints, instead of excluding physical reference points in the outer area, a logic for generating 'Fake References' is introduced that mathematically extends and copies the coordinates of the real reference points inside outward, thereby perfectly performing spatial color interpolation (IDW, etc.) without divergence errors. Furthermore, as a specific embodiment, the present invention incorporates a detachable plasma separation membrane upper structure that suppresses red blood cell interference when whole blood is injected, thereby removing the upper contaminants entirely after a chemical reaction and taking a single shot of only the exposed lower support with a smartphone, thereby achieving precise quantitative readings at the level of medical devices without the need for expensive dedicated equipment.

The present application relates to the technical field of biological detection pretreatment device, and more particularly to a red blood cell folate pretreatment detection device, which comprises a magnetic stand, a reaction tube is placed on the magnetic stand, and a multi-gear magnetic separation mechanism for providing different magnetic field strengths in the magnetic adsorption stage, the washing stage and the resuspension stage is arranged on the magnetic stand, and the multi-gear magnetic separation mechanism comprises a lifting flat plate movably arranged in the magnetic stand. The lifting flat plate drives the cylindrical permanent magnet to approach or move away from the bottom of the reaction tube, and the three groups of rotating clamping blocks are matched to realize quick switching of gears. The strong magnetic gear makes the permanent magnet closely adhere to the bottom of the tube, and can quickly capture magnetic particles. The weak magnetic gear makes the permanent magnet maintain a proper distance, which can not only maintain adsorption but also avoid the agglomeration of magnetic beads. The zero magnetic gear makes the magnetic field basically disappear, which is convenient for resuspension. The three gears correspond to the core steps of pretreatment respectively, and the whole process is completed by one machine, so that the sample does not need to be transferred between different devices, and the detection efficiency and repeatability are effectively improved.

This invention discloses a method, apparatus, storage medium, and device for identifying full-path blockage in dual-impedance channels. The method includes: establishing a functional relationship between the voltage of a red blood cell impedance channel and the voltage of a white blood cell impedance channel; obtaining the current red blood cell impedance channel voltage and the current white blood cell impedance channel voltage at the current temperature; obtaining the target white blood cell impedance channel voltage based on the current red blood cell impedance channel voltage and the functional relationship; determining the absolute value of the difference between the current white blood cell impedance channel voltage and the target white blood cell impedance channel voltage; if the absolute value of the difference is greater than or equal to a first preset threshold and the difference is less than 0, then outputting full-path blockage information for the red blood cell impedance channel; if the absolute value of the difference is greater than or equal to the first preset threshold and the difference is greater than 0, then outputting full-path blockage information for the white blood cell impedance channel. This method can ensure that the voltage of the impedance channel is independent of temperature and can accurately identify whether full-path blockage has occurred in the impedance channel.

A method for staining reticulocytes includes staining reagent A and stabilizing reagent B; stabilizing reagent B is used M seconds after staining reagent A is mixed with the sample; M is greater than or equal to 20 seconds; staining reagent A includes staining agent A10, adjusting salt C10, and water; staining agent A10 is neomethylene blue; the concentration of neomethylene blue in staining reagent A is 0.8 × 10⁻⁶. ‑4 g / ml ~ 8.0 × 10 ‑4 g / mL; adjusting salt C10 is used to provide a low osmotic pressure environment and a pH range of 7.0–8.0; stabilizing reagent B includes staining stabilizer B10, adjusting salt D10, and water; the staining stabilizer B10 in stabilizing reagent B includes aldehydes. A unique two-step reagent formulation and method are used to achieve rapid staining of reticulocytes in a step-by-step manner, achieving successful staining in just 20 seconds, and the staining effect can be maintained for a sufficiently long time, exceeding 120 minutes.

This utility model relates to the field of comprehensive utilization technology of livestock and poultry blood, and discloses a centrifugal separation device for hemoglobin in livestock and poultry blood. It includes a base, an outer shell on top of the base, a motor mounting cavity inside the base, a first motor inside the motor mounting cavity, and a rotating shaft connected to the output end of the first motor. The rotating shaft extends to the inner bottom of the outer shell and connects to a rotating disk, with a central shaft clamped onto the rotating disk. In this utility model, red blood cells from livestock and poultry blood can be separated by centrifugation using a centrifugal device. The red blood cells are then broken down by a top stirring device that shears and precipitates them. After breaking down the red blood cells, the stirring device is removed, and the supernatant is extracted by centrifugation. This utility model has a simple and compact structure, and can continuously extract hemoglobin from livestock and poultry blood with high extraction efficiency.

Classes of quinones for use in the treatment of red blood cell enzymopathologies. The treatment increases ATP in non-mitochondrial cells. Thus, the present invention relates to classes of quinones for use in the treatment of diseases or conditions associated with or caused by defects in red blood cell glycolysis.

This invention belongs to the field of medical image processing technology, specifically relating to a method for identifying, counting, and determining the proportion of target-shaped red blood cells. The method involves acquiring high-resolution digital images of bone marrow or peripheral blood smears under an oil immersion lens; inputting these high-resolution digital images into a dual-task deep learning detection model, which simultaneously performs pixel-level semantic segmentation of individual red blood cells and their intracytoplasmic target structures; based on the localization and pixel-level semantic segmentation, determining the presence and classification of each red blood cell, counting the number of target-shaped red blood cells and the total number of red blood cells, and determining the proportion of target-shaped red blood cells using a proportioning algorithm; based on the proportion of target-shaped red blood cells, fusing red blood cell parameters to generate morphological integrated indicators, and outputting a diagnostic report. This solves the problems of lacking standardized image acquisition procedures and difficulty in accurately distinguishing between target and non-target-shaped red blood cells in existing technologies.

We disclose here the nano-enabled delivery of highly potent 5-hydroxymethyl furfural (5-HMF) to sickled blood cells in patients. 5-HMF, a superior antisickling agent, has been formulated to address the limitations of existing treatment modalities arising from disfavorable pharmacokinetics of the drug compound. Specifically, two complementary 5-HMF prodrugs are integrated as a ‘protected’ biocompatible composite nanoparticle designed to readily fuse with RBCs and be amenable to transdermal delivery (5-HMF-grafted-phospholipid layered over a sucrose-derived graphitic carbon dot core). These RBC-targeted, protected, biocompatible, self-assembled 5-HMF prodrug nanoparticles for sickle cell disease are the first in class technology for offering a treatment for sickle cell disease which has improved potency, targeted payload delivery, and extended release with the red blood cells with enhanced pharmacodynamic effect in a treated patient.

A particle sorting apparatus includes a determination unit that performs sorting determination of a particle, the determination unit performing the determination using rule data that defines relationships between particles in terms of particle populations to which the particles belong and particle populations to which different particles within a predetermined range around the particle belong, the particle population to which the particle that is a target of the sorting determination and the different particle within the predetermined range can belong including (a) a particle population of particles to be sorted, (b) a particle population of particles that are not sorted but are ignorable in the determination, and (c) a particle population of particles that are neither the particles to be sorted nor the ignorable particles, the ignorable particles including red blood cells.

There are provided polypeptides that include an Activin receptor type IIB (ActRIIB) ectodomain (ECD) variant. In some embodiments, a polypeptide of the disclosure includes an ActRIIB-ECD variant fused to an Fc domain moiety. The disclosure also provides pharmaceutical compositions and methods of using the polypeptides to treat diseases and conditions associated with TGFβ superfamily ligand signaling, such as metabolic disorders, diabetes, obesity, cardiometabolic disease, pulmonary hypertension, fibrosis, muscle weakness and atrophy, bone damage, and / or low red blood cell levels (such as anemia).

The application provides application of a TADA2B gene in promoting terminal differentiation of erythroid cells, and relates to the technical field of biotechnology. The application comprises (I) promoting terminal differentiation of erythroid cells; (II) preparing a product for promoting terminal differentiation of erythroid cells; (III) promoting differentiation of hematopoietic stem cells into red blood cells; (IV) preparing a product for promoting differentiation of hematopoietic stem cells into red blood cells; and (V) preparing a drug for treating, preventing and / or alleviating a pathological condition or disease related to red blood cell deficiency of a subject. The application alleviates technical defects of existing in-vitro production of red blood cells.

The present invention relates to the use of light microscopy for reticulocyte maturity classification of stained reticulocytes. In particular, the present invention relates to a method for reticulocyte maturity classification from a whole blood sample, comprising: staining the sample with a supravital agglutination stain reagent or a fluorescent agglutination dye; illuminating the stained sample with a light beam to detect reticulocytes; determining for each reticulocyte the parameters (i) the fraction of reticular area (Ar) relative to the whole cell area (Ac) (Λ) and (ii) the fraction of reticular perimeter (Ur) relative to the reticular area (Ar) (Γ); and classifying the reticulocyte maturity into one of four main maturity classes according to the determined Λ and Γ values.

The present application belongs to the field of gas detection, and relates to a combined detection tube for detecting alcohol concentration in blood. The detection tube is composed of a sample adding tube and a detection tube. The sample adding tube is internally provided with non-woven fabric, filler and stopper, which can effectively remove red blood cells and absorb moisture when the sample passes through, so that alcohol substances are gasified and diffused into the detection tube under the action of carbonates. The detection tube is further provided with a multi-layer structure including non-woven fabric, filler and different color indicating glue, so that after the oxidation-reduction reaction of alcohol substances and Cr(VI), the alcohol concentration can be directly reflected according to the color change (orange to blue) gradient, and qualitative and quantitative analysis in the range of 0.2-2.0 mg / mL can be realized. The whole detection process takes less than 8 minutes.

1. Name of the product in this design: Horizontal ultra-low temperature storage box (2). 2. Application of this design: This design is intended for use in hospitals, universities, research institutions, blood banks, disease control centers, livestock farms, pharmaceutical factories, chemical reagent factories, electronics factories, and other institutions to preserve medicines, vaccines, blood, plasma, viruses, bacteria, red blood cells, white blood cells, skin, bones, bacteria, semen, biological products, ocean-going products, electronic devices, and special materials that require special storage temperatures. 3. The key design feature of this product is its shape. 4. The image or photograph that best illustrates the design's key points: a 3D model.

The present application relates to the technical field of medical quality control products, in particular to a quality control substance for lymphocyte subset analysis and a preparation method thereof, comprising human monocyte simulation particles, human granulocyte simulation particles, human lymphocyte simulation particles, human red blood cell simulation particles, human platelet simulation particles and a preservative, wherein the human monocyte simulation particles, the human granulocyte simulation particles, the human lymphocyte simulation particles, the human red blood cell simulation particles and the human platelet simulation particles are added into the preservative, and mixed according to the concentration of human particles to obtain the quality control substance for lymphocyte subset analysis. Compared with the prior art, the quality control substance for lymphocyte subset analysis and the preparation method thereof have the advantages of simple operation, small batch difference and good stability, and can be applied to the field of quality control of flow cytometry methodological lymphocyte subset analysis.

The application discloses a preparation method of a bone formation inducing biological additive based on red blood cell-derived apoptotic vesicles and application thereof, and the preparation method comprises the following steps: a, red blood cell apoptosis induction treatment; b, collection and purification of red blood cell-derived apoptotic vesicles RBC-ApoEVs through series differential ultracentrifugation; c, characterization and quality control of the apoptotic vesicles; d, acquisition, culture and basic identification of SHED; and e, determination of 0.5 mu g / mL as the optimal bone formation inducing synergistic concentration of the red blood cell-derived apoptotic vesicles RBC-ApoEVs. The bone formation inducing biological additive prepared by the preparation method can be used as a safe and efficient bone formation inducing synergistic agent, and can solve the problem that "proliferation promotion" and "differentiation promotion" are difficult to be coordinated, and can also avoid the dependence on exogenous growth factors and related risks. The bone formation inducing biological additive can be applied to in-vitro induction of SHED bone differentiation, bone regeneration cell suspension and bone tissue engineering compound.

Class of quinones for use in the treatment restoring, normalizing and / or improving ATP in cells lacking mitochondria, such as red blood cells. Specific example is use in the treatment of red blood cell enzymopathies, especially in the treatment of defects in red blood cell metabolism such as defects in red blood cell glycolysis. The treatment increases ATP in non-mitochondrial cells. Thus, the present invention relates to a class of quinones for use in the treatment of diseases or conditions associated with or caused by defects in red blood cell glycolysis.

The application relates to the technical field of immunomagnetic bead preparation, in particular to a composite antibody immunomagnetic bead with a ring-shaped magnetic attraction structure and a preparation method and application thereof. After being cleaned and activated, the magnetic bead is coupled with at least two kinds of composite anti-red blood cell antibodies with different antigen epitopes, and after being sealed, free anti-red blood cell antibodies are added to prepare a vacuum freeze-dried product; through a matched ring-shaped magnetic attraction structure, a plurality of trapezoidal permanent magnets are assembled according to a Halbach array, the periphery is fixed through a ring-shaped stainless steel sleeve, and a uniform radiation ring-shaped magnetic field is formed; when applied, a mixed solution of whole blood and the magnetic bead is placed in the center hole of the structure and is magnetically attracted for 10-90 seconds; the composite antibody immunomagnetic bead with the ring-shaped magnetic attraction structure and the preparation method and application thereof can significantly improve the red blood cell capturing and separation efficiency, shorten the separation time, reduce the magnetic bead consumption, facilitate plasma suction, cover most clinical scenes, and reduce the industrialization cost.

This application relates to the delivery of substances to enucleated cells. Current subject matter includes methods, systems, articles, and techniques for delivering substances to enucleated cells such as red blood cells. Using rapidly deformable microfluidic systems, loading red blood cells with macromolecules of varying sizes has been demonstrated. While delivery to some mammalian cells, such as cancer cell lines and fibroblasts, using this technique has previously been shown, these designs are incompatible with RBCs, which have significantly different physical properties. Successful delivery to red blood cells can be achieved by using smaller contractile portion sizes, high speeds, and different buffer solutions. By achieving robust delivery to red blood cells in a simple and scalable manner, the current subject matter can be implemented in a variety of applications, such as delivering substances for studying red blood cell diseases and / or using red blood cells as a therapeutic platform. Related devices, systems, techniques, and articles are also described.

The present application relates to the field of biotechnology, in particular to human bladder tissue digestion solution and application thereof.The digestion method of the present application can dissociate human bladder tissue cells while keeping the cell viability at more than 90%, and the amount of harvested cells is also obviously improved.Sufficient cell amount is the prerequisite for optimization treatment such as red blood cell lysis and dead cell removal.The single cell suspension obtained by the method has high viability, low clumping rate, high nucleated rate and less impurities, which can provide a basis for obtaining high-quality gene expression data in human bladder tissue single cell sequencing experiment.The present application can quickly obtain human bladder tissue primary cell suspension (sufficient cell amount, high viability, low clumping rate and less impurities) meeting the requirements of single cell experiment within 2-4 hours.

The utility model discloses a red blood cell folic acid detection kit, including detection kit, box cover, the hinge of box cover is in detection kit top, and the inside of detection kit is equipped with detection reagent bottle storage and detection storehouse. The detection reagent bottle storage is provided with multilayer stackable detection reagent bottle storage board in, is used for stable storage detection reagent bottle, and is equipped with the detection placement board of liftable in detection storehouse, and its height can be adjusted through electric telescopic link, and is equipped with ultraviolet germicidal lamp, and is convenient for detection and disinfection. This reagent box has the advantages of stable structure, moisture -proof bacteriostatic, flexible operation etc. improves the accuracy and convenience of red blood cell folic acid detection.

The utility model discloses a kind of platelet-rich plasma preparation devices, it is related to medical instrument technical field;To solve the problem that blood separation mode is easy to pour red blood cell, inconvenient operation, and platelet recovery rate and efficiency are lower, the following technical scheme is proposed: it includes storage cavity, separation cavity and handle, the lower end of separation cavity is arranged in storage cavity, the lower end of storage cavity rotatably sets handle, the upper end surface of separation cavity is clamped with upper cover, and the lower connecting end of storage cavity is equipped with lower cover;Storage cavity is nested in reversal portion, and the cavity of storage cavity is communicated with separation cavity through reversal portion, and the lower chamber of storage cavity is equipped with piston, and the lower end surface of piston is abutted with the thread rod end surface of handle.The utility model realizes the complete independent separation storage of platelet-rich layer, plasma layer and red blood cell layer after whole blood centrifugal separation.Improve work efficiency and recovery rate, also ensure the safety and reliability of blood treatment, with extensive application prospect and popularization value.

The application relates to the technical field of biomedical engineering, and discloses a method for constructing an ex vivo normal-temperature perfusion repair model based on a discarded liver. The method constructs a double-path closed-loop perfusion system, applies a shear stress flow field with low-frequency fluctuation characteristics to a portal vein loop to maintain endothelial function, loads a composite flow field obtained by superimposing low-frequency pulsation and high-frequency micro-shaking on a hepatic artery loop, and significantly reduces microcirculation flow resistance by using the thixotropy and shear thinning characteristics of oxygen-containing perfusion liquid. The system further realizes physical layering of red blood cells and lipids by using acoustic radiation force of a standing wave acoustic flow control layered cavity, and automatically guides a high-viscosity fat-rich flow layer into an adsorption module for specific purification by cooperating with a thixotropic passive shunt valve based on fluid viscosity feedback. Temperature-gas coupling adjustment based on a metabolic temperature coefficient is performed in a rewarming stage. The application solves the problems of microcirculation non-recurrent flow and hyperlipidemia clearance by coupling of multi-dimensional physical fields, and realizes effective repair of the discarded liver.

This invention provides a SNP site, identification kit, identification method, and application for the Jk(a+w) blood type, which triggers immune hemolytic transfusion reactions. The SNP site is the c.613C>T mutation at position 613, starting from the start codon of the SLC14A1 gene coding region. In the proband, the c.613C>T mutation was detected in one allele, while the other allele carried other known inactivating mutations. The allele carrying the c.613C>T mutation resulted in reduced expression of the Kidd protein in the proband's erythrocytes; the other allele, carrying other inactivating mutations, did not express the Kidd protein. The combined effect of these two mutated alleles resulted in the Jk(a+w) phenotype. This provides a genetic variation basis for the establishment of rare blood type banks and the assurance of transfusion compatibility.

Provided is a blood processing module for an automatic blood viscosity measurement device, and more specifically, a blood processing module for an automatic blood viscosity measurement device which scans blood collection tube information through scan, mixes a blood sample by rotating a blood collection tube 360° to completely prevent an erythrocyte sedimentation phenomenon that may occur during waiting time, and furthermore, can improve accuracy of blood viscosity measurement through uniform mixing of the blood sample by sucking and dispensing blood by using a pipette tip to achieve secondary mixing of blood.

1. Name of the product in this design: Horizontal ultra-low temperature storage box (1). 2. Application of this design: This design is intended for use in hospitals, universities, research institutions, blood banks, disease control centers, livestock farms, pharmaceutical factories, chemical reagent factories, electronics factories, and other institutions to preserve medicines, vaccines, blood, plasma, viruses, bacteria, red blood cells, white blood cells, skin, bones, bacteria, semen, biological products, ocean-going products, electronic devices, and special materials that require special storage temperatures. 3. The key design feature of this product is its shape. 4. The image or photograph that best illustrates the design's key points: a 3D model.