Micro-fluidic chip device for circulating tumor cell (CTC) detection

Abstract

The invention provides a micro-fluidic chip device for circulating tumor cell (CTC) detection. According to the device, through an integrated micro-fluidic chip mode, functions of blood sample pretreatment, red blood cell lysis, leukocyte screening, circulating tumor cell enrichment, cell marker staining, cleaning and detection, CTC collection and the like are realized. Various functional units ofthe micro-fluidic chip are corresponding to the chip structure. Each solution tanks are connected through microfluidic channels, diameter of the channel matches the structure of researched cells, andfunctional transport of a cell solution in the channel is guaranteed. The device of the invention is used for screening, detecting and collecting circulating tumor cells in peripheral blood sample solutions of patients, providing clinic treatment and guidance and conducting quantitative statistics of circulating tumor cells, and also can be used for downstream single-cell genome and transcriptomeanalysis. The device of the invention is simple to operate, has advantages of saving time cost, realizing automatic detection and raising detection efficiency and analysis efficiency, and has vitallyimportant clinical examination value.

Description

technical field [0001] The invention relates to microfluidic analysis and detection technology in the field of biomedical engineering, in particular to a microfluidic chip device for detecting circulating tumor cells (CTC). Background technique [0002] The study of tumor single-cell transcriptome must be based on the library construction technology of single cell or even subcellular structure. In the face of the complex tissue environment in vivo and the need for sorting cell subsets, a reliable and stable cell isolation methodology has become the first step for successful single-cell RNA sequencing of tumors. CTC (Circulating Tumor Cells, circulating tumor cells) has diagnostic value, but its content in the blood of tumor patients is extremely low, so the sensitivity and specificity of its sorting are also high; in recent years, single cells in solid tumor tissues It also has the scientific value of tumor heterogeneity and immune microenvironment and has become a research...

AI Technical Summary

Problems solved by technology

However, because the above-mentioned equipment is not only very expensive, but also due to technical limitations and other reasons, most of the equipment can only count, and cannot be separated and used for other downstream applications. Therefore, only the CTC-Chip series is currently used in the clinical practice of single-cell RNA sequencing. Research

Moreover, these techniques are very time-consuming for the isolation of CTCs and cause certain damage to the cells, and they cannot meet the requirements of next-generation sequencing techniques for tumor transcriptome analysis.

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