160 results about "Cell marker" patented technology

The invention discloses a treatment response prediction method and system, a storage medium and a terminal, and the method comprises the steps: carrying out the heterogeneity analysis and cell division of first single cell sequencing data of a patient through employing a clustering algorithm, and obtaining a plurality of corresponding cell subgroups; performing feature extraction and biomarker recognition on all the cell subgroups to obtain corresponding cell subgroup features; wherein the cell subset characteristics comprise a cell development trajectory and a plurality of cell markers; and inputting the clinical information and the cell subset characteristics of the patient into the treatment response prediction model, so that the model performs multi-factor analysis and outputs a treatment response prediction result of the patient for each treatment scheme. By adopting the embodiment of the invention, the tiny key difference between the cells is captured, the cells are divided based on the difference between the cells, and then feature extraction and biomarker recognition are performed on each cell subset, so that the specific gene expression modes of different cell subpopulations corresponding to diseases can be recognized, and the treatment response prediction accuracy is further improved.

The invention discloses a sebastes schlegeli testis cell line as well as a construction method and application thereof, and belongs to the technical field of marine fish cell culture. Primary culture and subculture are carried out on sebastes schlegeli juvenile fish testis tissues, so that the sebastes schlegeli testis cell line capable of being continuously subcultured in vitro for a long time is established. The sebastes schlegeli testis cell line established by the invention is in a fibroblast shape, has a good growth curve, and has relatively strong multiplication capacity. The lentiviral vector can significantly express the stromal cell marker gene temp2, can transfect the cell line, and can be subsequently applied to research of gonad development related functional genes. At present, the cell line has been subjected to in-vitro continuous passage for more than 60 generations, can be subjected to cryopreservation, and is high in recovery survival rate. Establishment of the cell line provides a stable and excellent experimental material for research of fish gonad development genes.

The invention discloses a gastric cancer multi-omics marker detection method, system and device, and the method comprises the following steps: S1, collecting a public database open-source space transcriptome, a single cell sequencing sample and bulk-RNAseq data for pre-processing, and collecting a primary tissue sample of a gastric cancer patient in the center for data processing; s2, integrating different modal data samples to obtain a patient label of an input end, and constructing a marker detection model and training the marker detection model; and S3, extracting a target feature value from the input external pathological section by using the trained model, and generating a diagnosis prediction result. Through multi-modal data chimerism, algorithm optimization and AI system development, subpopulation cell marker proportion prediction and prognosis diagnosis and marker evaluation with population prognosis information are realized, and an integrated diagnosis scheme for breaking through molecule-space-prognosis information is constructed.

The invention relates to the technical field of biological information, in particular to a brain glioma microenvironment formation key molecular mechanism analysis method, which comprises the following steps: calling expression data to analyze a cell marker sequence to construct a segmentation interval, calculating a candidate factor expression direction to judge trend consistency, identifying expression aggregation difference to construct a split node section, and constructing a subsection; according to the method, partitions are constructed on the basis of marker expression syn-position, judgment is carried out in combination with candidate factor expression directions and marker trends, factor collaboration features are constructed according to the number of trends consistent times, and the semantic sorting information is generated by analyzing the channel change trends to generate drift scores, evaluating multi-label output stability screening key factors and analyzing literature word order positions. Identifying and expressing an aggregation and split structure, guiding a functional pathway to perform trend analysis in a scoring interval and construct a dynamic trajectory, judging label stability and empowerment according to pathway scoring difference, and determining a factor semantic position in combination with a literature word order structure to realize integrated support of regulation and control information and literature evidence.

The invention discloses a spatial omics single cell data acquisition method and device, electronic equipment and a storage medium. The method comprises the following steps: acquiring a dyeing image of a biological sample, and acquiring a gene expression visual image of the biological sample; carrying out registration on the dyed image and the gene expression visualization image to obtain a registered image; performing cell segmentation on the registered image to obtain a cell mask pattern; determining cells of background gene molecules of unknown cells in the gene expression visualized image according to the cell mask graph, and marking the cells to obtain a space group molecular expression matrix with cell markers. Compared with the prior art, the method has the advantages that high-precision spatial single cell data is obtained through the processing flows of gene expression matrix graph, splicing, registration, segmentation and correction.

In one embodiment, a method is disclosed to determine one or more biological cells of interest to identify and count in a mixed biological sample fluid with differing biological cells. The method can include selecting cell markers associated for biological cells to which conjugated antibodies can attach with differing fluorescent dyes; displaying a panel builder graphical user interface window to display a co-expression matrix by biological cell type to assist in selecting cell markers to assign co-expression; and selecting cell markers to assign co-expression with an input device.

The invention provides a method and a device for extracting cell marker genes in literatures, which are characterized in that statement texts are extracted from the literatures, entity statement texts are screened out and cleaned, target entity statement texts are obtained and input into a text classification model, and target statement texts conforming to cell marker gene sentence patterns are screened out; and pairing the core segmented words and the dependent segmented words or the dependent segmented words in the target statement text to obtain segmented word relation pairs, screening and filtering to obtain target segmented word relation pairs meeting conditions, and taking the target segmented word relation pairs as cell marker genes. According to the scheme, the entity statement text containing the cell name entity and the gene name entity at the same time is screened out through the entity library, the target statement text conforming to the cell marker gene sentence pattern is screened out through the text classification model, and the core segmented words and the dependent segmented words or the dependent segmented words in the target statement text are paired; therefore, the purpose of rapidly and efficiently extracting the cell marker gene from the literature is achieved.

The instant disclosure provides reagent compounds, and antibody and oligonucleotide reagents, for use in a variety of assays, including immunoassays and nucleic acid hybridizations. The reagent compounds comprise a bridging antigen or bridging oligonucleotide and a latent crosslinker moiety, such as a tyramide moiety. The bridging antigens are recognizable by the antibody of a corresponding antibody reagent with high affinity, and the bridging oligonucleotides are complementary to the oligonucleotide of a corresponding oligonucleotide reagent. The antibody reagents and oligonucleotide reagents also comprise a crosslinker activation agent, such as a peroxidase enzyme. Reaction of the reagent compounds with the crosslinker activation agent results in the amplification of signal in assays for target cellular markers, including cellular antigens and nucleic acids. Also provided are detectable antibodies specific for the bridging antigens, kits comprising the reagent compounds and antibody and oligonucleotide reagents, methods of signal amplification using the compounds and reagents of the disclosure, methods of preparation of the compounds and reagents, and compositions comprising the compounds and reagents.

The invention discloses an imitated intercorial nano preparation for treating acute respiratory distress syndrome, and belongs to the technical field of biological medicine. The internalized nano preparation comprises an active component, a liposome phospholipid bilayer and a target head, wherein the active component consists of an ROS (reactive oxygen species) removing drug and an anti-inflammatory drug. According to the invention, efficient and accurate targeting of the nano-preparation is realized by innovatively applying an imitated interment effect, an apoptotic cell marker is modified on the surface of the liposome, and an'eat me 'signal is released to simulate a natural biological process that macrophages clear apoptotic cells to be recognized and swallowed by alveolar macrophages, so that efficient and specific targeting is realized, and the targeting effect of the nano-preparation is improved. Meanwhile, up-regulation of PS receptor expression of macrophages at the inflammation part further enhances the targeting effect, and a new way is provided for efficient delivery of acute respiratory distress syndrome medicine preparations and remodeling of pathological microenvironment.

A system and method for analyzing mechanical properties of biological tissues combines light-sheet fluorescence microscopy with mechanical perturbation capabilities. The system integrates a MEMS-based compression device, high-speed light-sheet scanner, and digital image correlation analysis to characterize viscoelastic properties of three-dimensional cellular structures. The system enables quantification of spatially-resolved mechanical characteristics and cellular responses through volumetric strain analysis, correlating mechanical properties with fluorescence-based cellular markers. Implementation with maternal-fetal interface models demonstrates capabilities in analyzing tissue mechanics during cellular invasion processes. The system provides applications in studying mechanical interactions in biological processes including embryonic development, cancer metastasis, and wound healing. The disclosed methodology enables unprecedented analysis of mechanical property dynamics at cellular interfaces in three-dimensional tissue environments.

The invention discloses a specific disease iconography feature extraction method and system based on deep learning, and relates to the technical field of image analysis, and the method comprises the steps: collecting X-ray image data, stem cell markers, magnetic resonance imaging image data and dynamic ultrasonic image data of an osteoarthritis patient; performing standardization processing on the X-ray image data, the stem cell marker, the magnetic resonance imaging image data and the dynamic ultrasonic image data to obtain a standardized X-ray image data set, a standardized stem cell marker, a standardized magnetic resonance imaging image data set and a standardized dynamic ultrasonic image data set; a standardized X-ray image data set, a standardized magnetic resonance imaging image data set and a standardized dynamic ultrasound image data set are acquired. According to the invention, through multi-modal image data fusion and biomechanics-stem cell characteristic dynamic coupling, early accurate diagnosis and repair potential evaluation of osteoarthritis are realized.

The invention relates to the field of biomedicine, in particular to application of a biomarker in preparation of a product for detecting the aging degree of an individual. And the biomarker is any one or a combination of several of DDX24, EML4, IQGAP2, MT-ATP6, MT-CYB, MT-ND1, MT-ND4, MT-ND5 and PRDX6 (porcine reproductive and respiratory syndrome). Nine cell markers for aging evaluation and nine molecular markers for aging evaluation are obtained through single cell sequencing, flow cytometry sorting and QPCR identification of animals of different ages, the aging level of individuals can be rapidly evaluated by utilizing the markers, and the blank in the field of aging detection is filled.

The invention discloses a plaque typing system and device and a storage medium. The system includes a processor configured to perform a plaque typing method, the method including: determining a region of interest in an image of a target tissue sample, the image including cell marker information and plaque marker information; determining position data of at least two extracellular plaques included in the region of interest; according to the position data of the at least two extracellular plaques, a compactness index corresponding to the region of interest is determined, and the compactness index is determined based on the coverage proportion of the extracellular plaques and the distance between every two extracellular plaques; the coverage proportion of the extracellular plaques is the ratio of the area of the corresponding extracellular plaques to the sum of the areas of all the extracellular plaques included in the region of interest; and determining a plaque typing result corresponding to the region of interest according to the compactness index corresponding to the region of interest, wherein the plaque typing result is a dispersion type, a compact type or an intermediate type. The problem that existing plaque morphological description is low in accuracy is solved.

The present disclosure provides modified cells comprising a nucleic acid encoding an agent (e.g., a detection agent, a selection agent, or a detection agent and a selection agent) inserted at an endogenous proliferation gene or off-target cell marker gene, as well as compositions, methods, uses, and kits related thereto.

The present invention relates to a method for predicting a solid tumor immune cell therapy treatment response. The method comprises acquiring a tumor tissue section from a patient, and detecting the presence, number, proportion or density of one or more cell types in the tumor tissue section. A solid tumor immune cell therapy response is predicted by determining cell markers, such as α-SMA, CD3, CD4 or CD8, associated with the cell types. In addition, the present invention also relates to a kit used for predicting a solid tumor treatment response, including a reagent which detects expression levels of one or more cell types and cell markers in tumor tissue sections, and software and / or a user manual used for analyzing detection results. The method and kit provide an effective tool for clinical treatment, so as to optimize dosages and clinical regimens of immune cell therapy, thereby improving treatment response rates.

The present disclosure is concerned with substituted cyclopropyl carbamate and cyclopropyl sulfonamide compounds, pharmaceutical compositions comprising the compounds, and methods of using the compounds in the treatment of various cancers such as, for example, cancers comprising cells that express at least one Sox 2 stem cell marker. This abstract is intended as a scanning tool for purposes of searching in the particular art and is not intended to be limiting of the present invention.

1. The name of the design product: graphical user interface for computer cell marker gene statistics. 2. The use of the design product: the design product is used in electronic equipment to count cell marker genes. 3. The design points of the design product: the interface content of the graphical user interface. 4. The picture or photo that best shows the design points: front view. 5. The product involved in the design patent application is a graphical user interface, and the rear view, left view, right view, top view and bottom view are omitted. 6. Other circumstances that need to be explained: the interface of the product is a graphical user interface for cell marker gene statistics, which is a structured design of species, system, tissue, cell type and visualization design of species structure. The first column is species, the second column is system and tissue, the third column is cell type, and the fourth column is typical marker statistics of cells. Users can filter species, system, tissue and cell type through this interface, and finally view the typical marker statistics chart of cells.

The invention relates to the technical field of biomedicine detection, and discloses a tumor cell marker detection system for predicting the activation state of intracellular protein kinase. Comprising a marker detection module used for qualitatively detecting epithelial cell markers, mesenchymal cell markers, cell polarity markers and extracellular matrix related markers in tumor cells; the data processing module is used for performing cross validation on an epithelial cell marker, a mesenchymal cell marker, a cell polarity marker and an extracellular matrix related marker; the dynamic weighting module is used for performing dynamic weighting on different markers based on a complex system theory, and endowing epithelial cadherin with a higher weight; and the judgment module is used for determining whether epithelial intercellular substance transformation occurs or not according to the dynamically weighted marker data, and predicting the activation state of intracellular phosphoinositide 3-kinase alpha and the invasiveness of tumor cells based on the determination result of the epithelial intercellular substance transformation.

The invention discloses a method and a system for analyzing immune cell change of an HER2 variant lung cancer patient. The method comprises the following steps: collecting a peripheral blood sample and carrying out single-cell RNA (Ribonucleic Acid) sequencing; performing quality control, standardization processing, dimensionality reduction and clustering analysis on the sequencing data; carrying out immune cell subset annotation on the clustering result based on a preset immune cell marker; analyzing cell communication modes among different immune cell subpopulations by using a ligand-receptor database; performing quantitative statistics on the proportion of various immune cells based on sample grouping; and further performing subpopulation fine analysis, quasi-time sequence trajectory reconstruction and metabolic pathway activity evaluation on the CD8 + T cells to construct a comprehensive immune cell map. According to the method, the multi-dimensional characteristics of the peripheral blood immune cells can be presented at high resolution, and the comprehensive analysis of the functional state, the communication network and the metabolic activity of the immune cells is realized. According to the system and the device, automatic operation and visual display of the method can be realized. According to the invention, the immune monitoring efficiency can be improved, and an effective tool is provided for immune treatment effect evaluation and disease mechanism research.

The invention belongs to the technical field of biological medicine, and particularly relates to a new Spp1 + Carmil1 + and Spp1 + Cav1 + macrophage subgroup, a screening method and application in diagnosis and treatment of rheumatoid arthritis (RA). Aiming at the function limitation of a traditional M1 / M2 classification model, the invention proves the following new subgroup pathological interaction mechanism: osteoclast (ACP5 +) specifically releases TNFSF11 (RANKL), and is co-localized with Spp1 + Carmil1 + and Spp1 + Cav1 + subgroup space; the Spp1 + Carmil1 + subgroup secretes IL-18, and space interaction exists between the Spp1 + Carmil1 + subgroup and Treg (Foxp3 +) and Th17 (Il17a +) cells. The invention provides a novel cell marker and a targeted treatment strategy for accurate diagnosis and treatment of RA.

There is provided a modified cell expressing (a) a chimeric antigen receptor targeting GPC3 and / or CD19, and (b) a multi-specific antigen binding protein, variant or binding fragment thereof that binds one or more target, comprising a first antigen binding protein, variant or binding fragment thereof that binds to EpCAM (epithelial cell adhesion molecule) and a second antigen binding protein, variant or binding fragment thereof that binds to an immune cell marker, wherein the first antigen binding protein, variant or binding fragment thereof that binds to EpCAM comprises a heavy chain variable region and / or a light chain variable region comprise sequences as disclosed herein. Also disclosed are methods of producing / generating the cell as disclosed herein and methods of treatment employing the cell as disclosed herein.

The present invention relates to tumor-specific immune cell markers and their uses. Specifically, an immune cell marker combination is provided, comprising: (1) an immune cell activation marker, and (2) an immune cell inhibition marker, optionally further comprising (3) an intracellular marker, and / or (4) a tissue-resident memory marker. The marker combination of the present invention can more accurately identify and enrich tumor-specific immune cells, and the enriched cell population has better tumor reactivity and tumor killing function.

The invention discloses a recombinant rabies virus genome plasmid, a recombinant rabies virus and a preparation method and application thereof, and the preparation method specifically comprises the following steps: (1) introducing a sequence 1 and a sequence 2 into the recombinant rabies virus genome plasmid, so that the recombinant rabies virus genome plasmid has more selectivity in modification and application; (2) introducing a fluorescent protein gene and a functional probe into the recombinant rabies virus genome plasmid, so that the recombinant rabies virus genome plasmid has the functions of expressing the fluorescent protein and the functional probe; (3) preparing a corresponding recombinant rabies virus which can express fluorescent protein with high sensitivity for cell marking and can also effectively express a functional probe for functional research; the method has wide application value and market prospect in the aspects of neural circuit structure and function analysis, neuron sparse marking, vaccine and drug research and development, disease model establishment, neural network loss and reconstruction detection, virus replication or pathogenic mechanism research and the like.

A modified cell is provided that expresses (a) a chimeric antigen receptor targeting GPC3 and / or CD19, and (b) a multispecific antigen binding protein, variant or binding fragment thereof that binds to one or more targets, comprising a first antigen binding protein, variant or binding fragment thereof that binds to EpCAM (epithelial cell adhesion molecule), and a second antigen binding protein, variant or binding fragment thereof, which binds to an immune cell marker, wherein the first antigen binding protein, variant or binding fragment thereof, which binds to EpCAM comprises a heavy chain variable region and / or a light chain variable region comprising a sequence as disclosed herein. Also disclosed are methods of generating / generating cells as disclosed herein and methods of treating with cells as disclosed herein.

A method for detecting, on a detection support, at least one microorganism present in a sample to be analysed and revealed by means of at least one cell marker includes a first taking of an image (I0, I′0) before a step of controlled release of said markerand bringing this marker into contact with said microorganism, and at least one taking of an image (Ii) following this contact step, so as to detect said microorganism according to the change in the staining kinetics thereof, by means of a comparative analysis between the different images that have been taken. An automated detection device is capable of implementing the method and a detection support is capable of being used in the method and which is part of the device.

The present invention relates generally to the identification and isolation of human otic progenitor cells. More specifically, the present invention relates to a method of using cell markers to identify and isolate human otic progenitor cells from a mixed population of cells, methods of enrichment and production of human otic progenitor cells, and associated kits for use in identification and / or isolation of human otic progenitor cells, wherein the cell markers are selected from SSEA1 (CD15), disialoganglioside GD3, TRA-2-49 (liver / bone / kidney alkaline phosphatase), SSEA4, ganglioside GD2 and CD141.

The invention discloses a cerebrospinal fluid cell marking method and device, computer equipment and a storage medium, and relates to the technical field of cerebrospinal fluid cell marking, the cerebrospinal fluid cell marking method comprises the following steps: acquiring a cerebrospinal fluid cell image, and performing full-sheet display on the cerebrospinal fluid cell image, the cerebrospinal fluid cell image being an image obtained by performing full-sheet scanning on a cerebrospinal fluid cell smear; in response to a cell frame selection operation for the cerebrospinal fluid cell image, performing cell detection on a selected area, and performing frame selection positioning on cells detected in the selected area by using a square marking frame, the vertical distance between each edge of the square marking frame and the center of the cells being a preset distance; classifying the cells detected in the selected area by using a preset classification model to obtain classification information of the cells; obtaining color information corresponding to the classification information based on a preset mapping relation; and sequentially displaying the color blocks corresponding to the color information and the type names corresponding to the cells above the square marking frame.

The invention relates to the technical field of cell culture, in particular to a 3D culture and purification method for olfactory ensheathing cells and olfactory ensheathing cell spheres. The method comprises the following steps: carrying out isolated culture on a mixed solution containing primary olfactory ensheathing cells to obtain mixed cell spheres; incubating the mixed cell spheres on a low-adsorption culture plate to obtain purified olfactory ensheathing cell spheres; the surface of the low-adsorption culture plate is a hydrophobic surface. The method can avoid the use of unstable cell markers, and effectively realizes the separation and purification of olfactory ensheathing cells; the purified olfactory ensheathing cells have good biological activity, and research and medical application related to the olfactory ensheathing cells can be more effectively promoted.

Immune context scores are calculated for stage III colorectal tumor tissue samples using continuous scoring functions. Feature metrics for at least one immune cell marker are calculated for a region or regions of interest, the feature metrics including at least a density of human CD3+ cells in a region of interest including an invasive margin. A continuous scoring function is then applied to a feature vector, the output of which is an immune context score. The immune context score may then be plotted as a function of a diagnostic or treatment metric, such as a prognostic metric (e.g. overall survival, disease-specific survival, progression-free survival) or a predictive metric (e.g. likelihood of response to a particular treatment course). The immune context score may then be incorporated into diagnostic and / or treatment decisions.

The invention discloses a method for evaluating in-vitro lymphopoiesis inhibition of mesenchymal stem cells, and relates to the technical field of biological medicine, the method for evaluating in-vitro lymphopoiesis inhibition of mesenchymal stem cells comprises the following steps: S1, preparing an MSC cell suspension; s2, preparing a PBMC (peripheral blood mononuclear cell) suspension, namely preparing a commercialized mixed source PBMC with a built line; s3, MSC inoculation adherent culture is carried out; s4, carrying out CFSE dyeing and inoculation; s5, marking the PBMC proliferative cells by using the MSC: after culture is completed, adding a cell activity detection reagent, incubating in a dark place, detecting, and carrying out statistical analysis; according to the method, the sample size of the same batch of PBMCs is increased by adopting commercialized established-line mixed source PBMCs, so that the difference between batches is reduced; compared with a traditional evaluation method, the method provided by the invention can more comprehensively and reliably evaluate the in-vitro lymphocyte proliferation inhibition capability, so that a basis is provided for clinical application of mesenchymal stem cells.