111 results about "Cell smear" patented technology

The invention relates to an automatic micro imaging instrument used for detecting cast-off cells and a detecting method, in particular to an automatic analysis instrument of a uterine neck cell smear and a detecting method. The instrument comprises a microscope, a three dimensional automatic object stage, an automatic film loading device and a control system thereof, a light system, a stable system, a light filter, a computer, a display and a digital camcorder, etc. The detecting method comprises the following steps: the smear is analyzed and colored, then arranged on the automatic object stage which moves up and down to focus automatically, when the automatic object stage moves horizontally, the smear scanning is realized; cell images are amplified by the microscope, picked up and sent to the computer to carry out image processing and analyzing. The detected cells are filtered by two-value filtration to remove the noises, the area and the perimeter are measured, and the impurity is deleted, then the spectrum and other shape parameters of the cells are measured; the measured cells are classified with a pattern recognition algorithm into the cast-off cells, white blood cells, lymphocyte cells and the impurity; after a viewing field is finished, the cells move down automatically, auxiliary diagnostic data is obtained by the computer. The instrument and the detecting method have the advantages of high degree of automation, accurate analysis results and reliability.

The invention relates to a liquid-based cell smear manufacturing and dyeing machine utilizing principles of injection and pipetting, which is characterized by structurally comprising a machine base, a machine body, a worktable, a horizontal slide rail, a moving arm, a horizontal motor moving device, an up-down motor moving device and a microcomputer motion control system, wherein four groups of smear manufacturing and dyeing units and a kit are mounted on the worktable board; and 24 samples can be automatically manufactured into smears and dyed when the machine runs once. The liquid-based cell smear manufacturing and dyeing machine does not need a tedious specific gravity liquid gradient centrifugation step, a micropipe type liquid drawing system of a liquid-based cytology test (LCT) machine, or processes of vacuum negative pressure drawing and positive pressure cell transfer of a microporous filter membrane of a Thinprep cytology test (TCT) machine. Cell samples are drawn, evenly mixed and scattered by using a pipetting injector and sample reagents are transferred by utilizing an accurate quantitative liquid drawing and injecting function of the pipetting injector. Cells in a settling and smear-manufacturing bin are naturally settled on a sticky glass slide by relying on the gravity of the cells and then automatically dyed. The liquid-based cell smear manufacturing and dyeing machine has the advantages that the operation is simple, the cost is relatively low, and high quality liquid-based cytological smears can be rapidly manufactured.

The invention discloses a preservation solution for exfoliated cell and a preparation method which uses the preservation solution to process cell thin layer smear. The preservation solution for exfoliated cell comprises the components which are prepared by the volume proportion of 10 to 15 portions of methanol, 10 to 15 portions of alcohol, 1 to 2 portions of glycerin, 1 to 2 portions of glycol polyethylene, 10 to 12 portions of formaldehyde, 0.5 to 1 portions of glacial acetic acid, 10 to 20 portions of sodium chloride with 0.85 percent to 0.9 percent of mass concentration and 33 percent to 68.5 percent of phosphate buffer. The steps of the preparation method for cell thin layer smear are that: 1) the collected sample is put in the preservation solution for exfoliated cell for shaking; the sample is transferred to a centrifuge for centrifugal treatment after standing; 2) the supernatant is removed until 1ml to 2ml residue is left in the centrifuge tube; the cell suspension is obtained after uniform vibration; 3) 0.5ml to 1ml cell suspension is put into a smear preparation cup which is made into cell thin layer smear by a cell smear preparation machine.

The present invention discloses an apparatus for thin-layer cell smear preparation and in-situ hybridization, comprising at least one positioning device and a sealing device. The positioning device comprises at least one first opening. When the positioning device is set on a carrier device, the wall of the first opening and the carrier device form a cavity. The cavity is used to accommodate a cell suspension. The sealing device is provided on the positioning device for sealing the cavity to form an enclosed space.

The invention provides a tissue slice and cell smear sample treating device comprising a rack, a mounting plate, a swinging assembly and a three-axis moving mechanism, wherein the rack is fixedly provided with a test tube frame and a collection groove; the mounting plate is fixedly provided with a plurality of reaction cabins and the two ends of the mounting plate are pivoted on the rack; the swinging assembly comprises a swinging motor, a driving wheel which is synchronously coupled with a rotary shaft of the swinging motor, a driven wheel which is pivoted on the rack and is fixedly connected with one end of the mounting plate, and a synchronous belt which is synchronously wound outside the driving wheel and the driven wheel; the extending direction of the rotary shaft of the swinging motor is the same as the length direction of the rack; a movable end of the three-axis moving mechanism is fixedly provided with a suction tube; and the three-axis moving mechanism is used for driving the suction tube to move between a liquid suction position on the test tube frame and liquid feeding positions inside the reaction cabins. When the tissue slice and cell smear sample treating device is used for detecting, the reaction cabins are driven to swing back and forth, so as to guarantee that the reaction is sufficient enough, the moving-out speed of residual liquid can be increased, an operation procedure is simplified, and the accuracy and the efficiency are improved.

The invention relates to a method and a device for microscopically examining patient samples consisting of tissue or cells. Especially in the field of tumor diagnostics, typically the entire samples or selected sections thereof that are of special interest for test purposes are initially placed on substrate supports one by one or in groups. Said substrate supports are then placed individually or together with other substrate supports in holders in which the substrate supports are subjected to a defined sequence of process steps. According to the invention, the substrate supports that contain the patient samples are securely immobilized in the holders and can be rearranged differently for subsequent laboratory operations. For each laboratory operation, the patient samples thus form combined groups that are processed in parallel or are moved jointly.

The invention discloses a device and a method for making a liquid-based cytological smear in one step. The device comprises a sample centrifuge, a hanging basket which is installed on the sample centrifuge and a smear making bottle which is reversely placed in the hanging basket. The smear making bottle comprises a bottle cap, a bottle body and a filter screen which is installed between the bottle cap and the bottle body. Slide loading holes for loading glass slides are symmetrically arranged on the left and right of the side wall of the bottle cap. The size of the holes is slightly larger than the size of the glass slides. The method comprises that the steps that after the smear making bottle which contains sample cells is shaken, the glass slides are loaded in the smear making bottle and the smear is made in one step by conducting centrifugal treatment to the smear making bottle. The device and the method for making the liquid-based cytological smear in one step have the advantages that the effect of combining a liquid storage bottle and a centrifugal smear making chamber into one is realized, the centrifugal smear making process is simplified, the transfer link in the smear making process is omitted, the cell analysis, the cell settlement and the smear output process are completed in one step, and the stability of smear making quality is improved. As a device and a method for making cell smears, the device and the method for making the liquid-based cytological smear in one step can be widely used in the field of cell smear making.

The invention discloses a simple and quick preparation method of a thin-layer liquid-based cytology cell smear. The method is characterized by comprising the following steps: a, preprocessing a specimen; b, adding 5ml of adhesive separation liquid into a common centrifuge tube; c, centrifuging a sample mixture and discharging the precipitation from the centrifuge tube while reserving the supernate for use; d, placing a glass sheet with a written number into a long slot of a natural settling device fixing seat serving as corollary equipment for film making; e, placing a settling tube on the glass sheet; f, uniformly mixing the bottom sample left after the centrifugation of the step c, and adding the mixture into the settling tube; g, standing still and naturally settling for 15-30 minutes; h, rinsing the specimen with a washing liquid; i, fixing in 95% alcohol for 10 minutes, and performing Papanicolaou staining; and H, performing E staining or immunohistochemical staining and reading the film. Through the simple and quick preparation method of the thin-layer liquid-based cytology cell smear disclosed by the invention, the mucus, blood and inflammatory cells in the specimen can be separated by natural precipitation, the film is clear and the diagnosis accuracy is improved.

The invention provides a method and device for evaluating the satisfaction degree of a cell smear specimen. With the method and device adopted, the satisfaction degree of a cervical liquid-based cellsmear specimen can be evaluated intelligently and objectively. The method for evaluating the satisfaction degree of the cell smear specimen includes the following steps that: a cell recognition modelis constructed based on convolutional neural network technology; the cell recognition model is adopted to perform cell recognition on a test cell specimen image so as to obtain a recognition result; and whether the test cell specimen image is a satisfactory specimen image is judged according to the recognition result.

The invention provides an integrated type cell sedimentation and smearing device and a method for settling a cell and preparing a cell smear. The device comprises a cup body, covers and a slide, wherein the covers comprise an inner cover and an outer cover which are buckled with each other. The cup body is provided with an internally-arranged filtering cup which is connected with the inner cover to form a sealed structure; the inner cover protrudes upwards to from a protruding part; the protruding part and the inner cover are connected to form an integral structure which is through up and down; the inner cover is connected with the cup body; the protruding part and an inner cavity of the cup body are communicated to form a runner; upper and lower openings of the protruding part serve as a liquid outlet opening and a liquid inlet opening respectively; a slide fixing groove is formed in the top of the outer cover; the slide is inserted into the slide fixing groove to form a cell bearing structure adaptive to the liquid outlet opening. The invention further provides the method for settling the cell and preparing the cell smear. The device provided by the invention is practical and convenient, is low in cost and good in smearing effect, and is suitable for integrated full-automatic operation.

The invention discloses a glass slide adhesive. The glass slide adhesive includes 0.01-0.2% by volume of 3-aminopropyltriethoxysilane (APTES), 0.01-3% by volume of a hydrophilic reagent, 0.005-0.05% by volume of a stabilizer, 0.5-5% by volume of alcohol, and the balance ultrapure water. The glass slide adhesive makes cells and tissue slices be firmly adhered on a glass slide, prevents the shedding of the cells and the tissue slices from the glass slide because of peracidity, high temperature, high pressure and the like in a dyeing process, overcomes the disadvantages of bad adhesiveness and weak wettability of general adhesives when the general adhesives are used for the glass slide processing in practical work, is suitable for the tissue slices, routine cell smears, fluid-based cytologic preparation, and manual drops, and has the advantages of good glass slide adherence effect, long preservation time, low cost, and high popularization values.

The invention discloses a membrane type collecting system and method for liquid-based cell, belonging to the cell-collecting device and method of cell smear processing equipment. The prior art has the drawbacks of uneven quantity of cell collection, incompletion of cell and inconvenience for observation. The invention comprises a filtering device, a pressure feeding device for the filtering device, a controller. In the filtering device is provided a filter, which is a sealed vessel, and on which are provided with a liquid suction hole and an air vent; the liquid suction hole is covered by a filtrating membrane, and the air vent is provided with a windpipe to connect the filter with the pressure feeding device; on the filter is also provided with a filter pressure sensor to supervise the filter pressure; the filter pressure sensor and the pressure feeding device are connected with the controller. The invention has the advantages of realizing the basically constant quantity of cell collection, guaranteeing the completion of cells, removing the impurity, preventing from the cells deposit, as well as being convenient in use, observation and diagnosis.

The invention provides an identification method for an atypical abnormal glandular cell in a uterine neck cell smear. According to the method, glandular cells are divided into glandular cell clustersof clear structure, indivisible glandular cell clusters and single glandular cells; for the glandular cell cluster of clear structure, arrangement information and appearance information of the glandular cells serve as two inputs of a double flow neural network model; the single glandular cell and the indivisible glandular cell cluster are input to a convolutional neural network model; and the twomodels output an identification result. The method can be used to improve the identification effect for the glandular cells with obvious arrangement structures, identification is more complete, and misidentification and identification omission are reduced.

The invention discloses endocervical cell preserving fluid and a method for preparing an endocervical cell specimen. The endocervical cell preserving fluid comprises N-acetyl-3-cysteine, anhydrous alcohol, sodium chloride, disodium hydrogen phosphate, sodium dihydrogen phosphate, glacial acetic acid, concentrated hydrochloric acid and distilled water. The method for preparing the endocervical cell specimen comprises the following steps; soaking endocervical cells in a collection tube of the endocervical cell preserving fluid, uniformly oscillating, adding the endocervical cells in a smear device, arranging the smear device in a centrifugal machine for centrifuging, and taking a glass slide with a uniform thin-layer cell smear; and fixing the obtained glass slide with the cell smear by a fixing solution, dyeing, dehydrating, making the glass slide transparent, and finally, mounting to obtain a specimen smear for diagnosis. The endocervical cell preserving fluid and the method for preparing the endocervical cell specimen are reliable and practical, are low in cost, and have useful effects, thus being suitable for large-scale popularization.

A method of creating a layer of cells on a surface. In some embodiments, the method includes the steps of engaging a smear tool against the surface with an engagement force; flexing a portion of the smear tool to change an orientation of the smear tool with respect to the surface; moving the smear tool along the surface through a sample comprising cells suspended in a liquid; and adhering the sample to the surface to thereby create a layer of cells. Another method according to the invention creates a layer of cells on a surface by mixing the sample prior to and/or during the smear. The invention also includes systems for implementing the methods.

The present invention relates to the technical field of cytologic screening of tumorous lesions, particularly to an immunohistochemical staining method and applications of the immunohistochemical staining method in cervical tumorous lesion screening. The method comprises: obtaining a precipitate containing sample cells; obtaining a suspension containing sample cells; obtaining a cell smear or cell chip; and taking the cell smear or cell chip, immobilizing with an immobilize liquid, carrying out water washing on the cell chip with gradient alcohol, adding a repair liquid to carry out antigen repair, adding antibody or probe, adding a blocking solution to block the background, and carrying out immunohistochemical staining. According to the present invention, the false negative and false positive problem of the tumorous lesion cytologic screening can be solved, the workload and the burden of the pathologists can be significantly reduced, the immunochemistry and in situ hybridization staining background and non-specific coloring problem in the cell smear can be solved, and the per capita cost of the tumor screening can be significantly reduced.

The invention discloses a method for extracting residual nucleic acid from an HE (haematoxylin eosin) dyeing piece, and the method comprises the following steps of: decoloring the HE dyeing piece by using low concentration hydrochloric acid alcohol as a decoloring agent first, then, bleaching by an oxalate solution, and then extracting nucleic acid by enriching target cells. Compared with the prior art, the method provided by the invention can be used for effectively removing basic dye haematoxylin and acidic dye eosin, reducing the degradation of nucleic acid in the extracting and storing process effectively, and enriching and detecting needed target cells so as to extract residual nucleic acid by using all regular HE dyeing pieces such as a puncture tissue, a cell smear (including sputum and urea), gastrointestinal endoscope and the like and perform subsequent molecular neuropathologic detection, so that the method provided by the invention is good in practicality, and can be used for achieving good economical benefit and social benefit.

The invention discloses a cell smear dyeing machine structure. The structure comprises a casing, a control module and a core work module, wherein the control module and the core work module are arranged in the casing, and the control module is connected with the core work module. A dyeing machine is designed by focusing on low cost, small size, multiple functions and corrosion resistance, and thedesign of the core work module and the control module meets small-lot multi-batch co-dyeing demands; besides, the cell smear dyeing machine structure performs anti-corrosion protection on each structure of the core work module to prevent the core work module from being damaged. A fluid control module provides clear water required for cleaning and discharges waste liquids, a constant-temperature water bath module provides the constant-temperature demands of special dyeing vats, the temperature of dye liquor in the dyeing vats is controlled in a water bath manner, and an exhaust and lid openingfunction module is used for controlling auxiliary operations such as exhausting of waste gas, automatic opening and closing of a lid. Miniaturization and multiple functions of the dyeing machine are realized, and the service life of the device is notably prolonged.

The invention provides a rapid scanning method and system for cervical exfoliated cell smears. An area of a cervical exfoliated cell biological sample is set to be in a circular shape, cell scanning without repeating and missing is achieved through sample boundary judgment, the thickness of the smears is detected to formulate the adjusting step size of a microscope, definition judgment is carriedout on up to seven positions in each scanning area, a camera is called for photographing at the position with the highest definition, the cell number is recorded, and the scanning accuracy is achieved; focusing adjustment is made into a pst file, and correcting and editing are facilitated; and the cell number is recorded synchronously in the scanning process, and scanning is stopped after the target number is reached through logical operation. Thus, the speed and accuracy of the scanning process flow are improved.

The invention relates to a method for inspecting lymphocytic subgroup by a single clone antibody SPA red cell garland way. The method comprises the following procedures: first the red cell garland reagent is solved, then the circumference blood leukomonocytes are separated; leukomonocyte suspension is prepared by a Henke balancing salt solution free of Ca and Mg containing 20% serum of a newly borne calf; meanwhile, the sensitized red cell suspension is made into a sensitized red cell application liquid by using a Henke balancing salt solution free of Ca and Mg containing 20% serum of a newly borne calf; then the leukomonocyte suspension and the sensitized red cell application liquid are mixed by 1:1 V/V into a mixed suspension, which is centrifuged after placing still under 18-28 centigrade, then is placed still for 15-45 min under 4 centigrade; finally the mixed suspension is absorbed 15 or 20 times by a pipet having a sucking head, the absorbed suspension is made into cell smear, which is naturally dried, dyed and counted by a microscope with high magnification. Comparing with prior McAb-A-E method, the invention is of simplified detection process, shorter period, quantitative controlling, and can meet clinic detection.

The invention relates to an indirect method for using a monoclonal antibody SPA red cell rosette to detect lymphocyte subsets. The method firstly prepares a primary antibody sensitized red blood cell preservation solution and a secondary antibody sensitized red blood cell preservation solution; secondly, the peripheral blood lymphocytes are separated, and then a secondary antibody sensitized red blood cell application solution and a primary antibody sensitized lymphocyte suspension are prepared by a calcium-free and magnesium-free Hanks balanced salt solution which contains 20 percent newborn calf serum; then the primary antibody sensitized lymphocyte suspension and the secondary antibody sensitized red blood cell application solution are mixed by the volume ratio of 1: 1 to form a mixed suspension; a centrifugation is carried out after the mixed suspension is statically settled under 18 - 28 DEG C, and then the mixed suspension is statically settled for 15 - 45 minutes at the temperature of 4 DEG C; finally, a pipette which is provided with a suction head is used for blowing and sucking the mixed suspension for 8 - 15 times, then cell smears are prepared and calculated by a high-power lens after natural drying and dyeing. Compared with the original McAb-A-E indirect method, the invention is characterized by simplified inspection process, short period, quantized control and satisfying the requirements of clinical inspection.

The invention discloses a method for inspecting acid-fast bacilli by liquid-based thin-layer cell smears. The method comprises the following steps: collecting a specimen, and collecting 2-5ml of sputum of a subject; preparing a treating liquid, and preparing a sputum pretreating liquid for inspecting acid-fast bacilli by the liquid-based thin-layer cell smears; centrifuging, adding the sputum into the prepared sputum pretreating liquid, and vibrating for multiple seconds; preparing a section, absorbing multiple milliliters of the sputum at the bottom of a sputum pretreating liquid test tube by a sucker, putting the sputum into a section preparation bin, putting the section preparation bin into a section preparation machine, and taking out the section preparation bin to dry when the section preparation machine completes the preparation; and carrying out acid-fast staining and microscopic inspection. Compared with the prior art, the method provided by the invention has the advantages that the positive incidence for inspecting acid-fast bacilli by sputum thick smears is improved by tens of times; the tuberculosis infection source can be found out as soon as possible; the tuberculosis can be treated early if being found out early; the burden of patients is relieved; the tuberculosis expansion can be controlled.

The invention provides a staining kit of micro megakaryocytes of a bone marrow smear, and a using method of the staining kit. The staining kit comprises a reagent tube filled with a Polymer Helper reagent, a reagent tube filled with a PBS (Phosphate Buffer Solution), a reagent tube filled with an antigen repairing solution, a reagent tube filled with a hydrogen dioxide solution, a reagent tube filled with diaminobenzidine, a reagent tube filled with hematoxylin, a reagent tube filled with acid alcohol of 1%, a reagent tube filled with ammonium hydroxide of 1%, a reagent tube filled with xylene, a reagent tube filled with alcohol, a reagent tube filled with CD41 monoclonal antibodies, a reagent tube filled with CD61 monoclonal antibodies, a reagent tube filled with universal CD41 and CD61 immunohistochemical antibodies, and a packing box for packing the reagent tubes in a separated and concentrated manner. The staining kit disclosed by the invention overcomes the defect that form cell smears are judged according to subjective experiences, and the interference of false positive of single CD antibody staining to results is avoided, so that the detection accuracy is improved.

The invention relates to a device for collecting and separating a body fluid sample used for cell examination and a method therefor. The device comprises a sample collection container and a cell smear device, wherein the sample collection container comprises a container body with an opening at the top part; a lateral liquid discharge hole provided with a liquid discharge hole switch is arranged at the lower part of the side wall of the container body; the bottom of the container body is of the V shape or the turbination; and a T-shaped collection pipe is arranged at the bottom of the V shape or the turbination. The method comprises: adding various body fluid samples into the container body for resting, so that the visible components such as cells and the like in the body fluid samples canbe naturally precipitated into the collection pipe, and the liquid supernatant of the body fluid can be discharged by the liquid discharge hole; and then taking down the collection pipe and directly fixing the collection pipe on the cell smear device, and adopting a general centrifugation smear machine to prepare a cell smear directly. The device and the method can successfully collect large amount of the visible components such as cells, sediment and the like in the body fluid sample, can achieve the aim of obviously improving the relevance ratio of positive cells and can be widely used for the cytological examination of various body fluid samples.

The invention discloses a cancer screening and diagnosis method via staining quantification of cellular DNA by a fluorescent dye DAPI. According to the method, cell nucleuses in cell smears or cells and tissue sections are stained with DAPI as a dye, pictures are taken under a fluorescence microscope, and the DNA in each nucleus is relatively quantified according to the fluorescence intensity of each nucleus. Cells with abnormal DNA content (non-integer ploidy or ploidy number being greater than 4.5 or above) are suspected cancer cells. The number and morphology of the suspected cancer cells can be used for cancer screening and diagnosis. The dyeing method provided by the invention has the advantages of short dyeing time, simple operation and accurate quantification, is wide in application, can quantify the DNA of cells in cell smears, tissue sections and cultured cells, and can be used for screening cancer samples.

The invention belongs to the technical field of clinical medicine, and particularly to a sample preservation solution and a preparation method thereof, and a cell smear production method, wherein thesample preservation solution can be used for preservation and fixation of human tissue samples and cast-off cell samples in pathological diagnosis. According to the present invention, the specimen preserved with the sample preservation solution has characteristics of complete cell morphology, complete quantity, uniform distribution, clear chromatography, good adhesion and easy observation and diagnosis.