5 results about "Cell loss" patented technology

The utility model provides a multi-stage filter tube for pretreatment of a cell suspension, which is characterized in that each second single tube body serves as a middle tube body and is of a transparent cylinder structure, an external thread structure is arranged at the port of the top end of each second single tube body, and an internal thread structure matched with the external thread structure is arranged at the port of the bottom end of each second single tube body; every two adjacent single pipe bodies can be connected and installed through matched thread structures, and connecting pipe bodies which are sequentially arranged from top to bottom and provided with multiple stages of filtering hole diameters which are reduced step by step are formed. The first single tube body is used as the uppermost tube body; the third single pipe body is used as the lowermost pipe body; and the air pressure adjusting device is communicated with the first single pipe body. According to the utility model, through a multi-stage filtering structure, the aperture is reduced stage by stage, so that the filtering efficiency is improved, and the cell loss and the impurity residue are obviously reduced; the cell integrity is ensured by adopting lower pressure, the damage to the cells caused by overhigh impact force is effectively avoided, and meanwhile, the impurities are ensured to be fully removed.

The application belongs to the field of cell therapy process engineering, and discloses a CAR-NK cell high-yield culture method with dynamic process control. The method realizes the dynamic balance of nutrient supply and shear force in the culture system by introducing the strategy of combining culture stage regulation with one-time large-volume liquid replacement in a swing bioreactor. The method realizes online interception and rapid replacement of the culture medium in the high-density cell stage by using the built-in interception membrane of the reactor culture bag, thereby avoiding cell loss and nutrient depletion. Meanwhile, the method effectively maintains the CAR expression level and cell functional activity by cooperating with the optimized culture medium formula and the application of a transfection aid. The method has the advantages of simple operation and high repeatability, is suitable for various swing or closed reactor platforms, has good amplification performance and industrial application value, and can provide an efficient and stable technical scheme for the preclinical preparation and industrial production of CAR-NK cell immunotherapy.

The invention provides a cell freezing and embedding method for immunohistochemical staining, and belongs to the technical field of biology. The cell freezing and embedding method comprises the following steps: collecting cells; adding an embedding medium into the cells, and uniformly mixing the cells with the embedding medium; uniformly mixing the cells with an embedding medium, and freezing in liquid nitrogen; after the embedding medium is solidified, taking out the pre-embedded block; and carrying out secondary embedding on the pre-embedded block by using an embedding medium to obtain the cell frozen block for immunohistochemical staining. The cell freezing and embedding method disclosed by the invention is low in cell loss, low in cost and simple to operate; and the obtained frozen block slice is low in difficulty, is beneficial to long-term preservation and reuse, is better in quality and good in antigen preservation effect when being used for immunohistochemical staining, avoids false negative, and has a good application prospect.

The application discloses a method for preparing a cell block by using mucin emulsifier and application thereof, and the preparation method comprises the following steps: centrifuging a non-gynecological sample to obtain a first separation liquid, adding the first separation liquid into a formaldehyde-alcohol solution to centrifuge to obtain a second separation liquid, and adding the second separation liquid into mucin emulsifier to centrifuge to obtain a cell block. The preparation method is simple, the manufacturing cost is low, the cell block has high enrichment efficiency, cell loss is small, and the mucin emulsifier does not affect cell activity. The cell block prepared by using the mucin emulsifier has the following advantages: the appearance of the section of the cell block is complete, the section has a regular circular structure, the porosity is moderate, and the thickness is uniform; the application effect of the section in two kinds of staining is good: the hematoxylin-eosin staining has a clear background and clear contrast, cells are uniformly distributed in a circular area, cell morphology presents a stereoscopic effect, and the excellent rate of the stained section is 98.94%; the immunohistochemical staining has a clean background, and structures and cells are specifically dyed, and the excellent rate of the stained section is 99.30%, so that the cell block has a wide application prospect.