85 results about "Cell loss" patented technology

A method and apparatus for embedding cells that utilizes a flow-through embedding technique maximizes the efficiency of extractions and decreases time for embedding the cell fragments, minimizes cell loss, and automatically positions cell samples at the position in which a microtome blade will section them. The apparatus includes a cell flow pathway defined by an inflow tube for delivering cell fragments from a cell sample to a sample port. The sample port is in fluid communication with a tissue cassette having attached thereto a filter. The cell flow pathway is in communication with a reagent flow pathway for delivering the reagents through the sample port to the cassette. The apparatus is configured such that the application of pressure directs the cell fragments from the cell sample through the cell flow pathway, and effects delivery of the reagents through the reagent flow pathway. The apparatus produces an embedded cell block having concentrated cells near the plane of the block to be sectioned in a quick and efficient manner.

Method and apparatus are disclosed for treating congestive heart failure. The method includes relieving wall stress on a diseased heart by an amount to decrease a rate of myocardial cell loss. Further, the method includes pharmacologically encouraging a myocardial cell gain. Cell gain may be encouraged by cell replication, cell recruitment or inhibition of cell death. Further embodiments of the method include a passive cardiac constraint selected to reduce wall stress on the heart. An apparatus of the present invention includes a passive cardiac constraint and a pharmacological agent to encourage cell gain.

The invention provides a method for detecting or monitoring Alzheimer's disease and other forms of dementia using positron emission tomography (PET) or single-photon emission computed tomagraphy (SPECT) and radiolabeled, serotonin 5-HT_1A receptor-specific tracers (such as [F-18]MPPF, [F-18]FCWAY, [C-11]WAY-100635, and other radiolabeled compounds having agonistic or antagonistic effect on serotonin receptors), for detection or monitoring of pathological changes (i.e., neuronal cell loss) associated with dementia.

A linked list buffer circuit can be configured to store different linked lists. The buffer includes insert logic and an insert state machine to add ATM cells to the linked lists in the buffer. Extract logic and an extract state machine allow for the removal of ATM cells from the linked lists in the buffer. Because, ATM cells can have different levels of priority, programmable monitor circuitry is provided to monitor the linked lists in the buffer. The monitor circuitry keeps track of buffer and list capacity, and also keeps track of cell loss priority and delay priority cells of the buffer and the linked lists. This information is used to drop received cells if necessary. The linked list monitor circuitry can be configured based upon the linked list configuration of the buffer. The linked list monitor can be configured by changing threshold levels provided to counter/comparator circuitry. Assignments of internal counter circuitry are also programmable based upon the buffer configuration.

The present invention discloses a method of isolation, pooling and further culturing of Mesenchymal Stem cells (MSC) for clinical application. Present invention also discloses the method of establishing Master Cell bank, followed by Working Cell Bank from which the final therapeutic composition referred to as Investigational Product/Investigational Medicinal Product comprising of allogenic bone marrow-derived MSC is formulated for clinical applications. Present disclosure also discloses a robust manufacturing process for consistent production of clinical grade Mesenchymal Stromal cells (MSCs). The process enables production of highly viable potent cells. The process steps relating to preparation of media, cell seeding, harvesting are fine tuned to achieve consistency in cell yield, superior cell viability, purity, improved cell proliferation, high cell recovery, low HLA-DR expression, reduction in culture duration. The viability and purity of cells are further improved by optimized wash process without cell loss/cell stress. The disclosure further provides a method of cyrostoring MSCs at high cell density without affecting the viability of cells. It further provides economical means to store and transport at −80° C.

The invention relates to the biological cell technology field, particularly a method for separating and culturing miniature swine bone marrow-derived endothelial progenitor cells. The conventional EPCs culture method has the disadvantages of complexity and greatly reduced cell harvest rate by using immunomagnetic bead separation. The method in the invention comprises the steps of directly using myelomonocyte to conduct culture in vitro to obtain EPCs, adding growth factors in culture solution, planting in a definite density in a culture flask/ vessel or a glass sheet enveloped by fibronectin, and conducing appropriate culture to obtain EPC. The method with high repeatability simplifies the steps of the conventional culture method, effectively avoids unnecessary cell loss caused by the conventional separation and culture method, increases the pick-up rate of EPCs, and saves a large amount of funds. The method of the invention also prepares for clinical application in future, and can reduce the usage of bone marrow and provide a technology platform for curing traumatic or ischemic diseases by autotransfusion.

In order to uniformly sow cells on the culture surface of a cartridge-type closed culture vessel and remove bubbles contaminating in liquid culture medium in the course of automatic culture, an automatic culture equipment is provided with a flow-controlling mechanism section for solving the non-uniformity in cell distribution by, after filling up a culture space in the cartridge-type closed culture vessel, said cartridge-type closed culture vessel being in an upright position, with cell suspension, turning the cartridge-type closed culture vessel into a horizontal position, and then repeatedly supplying the cell suspension and sucking the same multiple times to thereby create a mixing flow in the cell suspension. In this process, the liquid in channels is efficiently sent by applying a reduced pressure and an elevated pressure respectively to a channel on one side and a channel on the opposite side, using two syringes and check valves connected to the channels, to thereby load forces to the liquid from both sides. The liquid can be sent without any cell loss by conducting the same operations of the flow-controlling mechanism section in a tank and in a cell bag.

The invention relates to a movable-type electric automobile charging management system and a method thereof. A station-level management server of a charging control center comprises VBee networking equipment, a data processing module and a historical data storage module. The station-level management server establishes a position relation function of a movable charger and an electric automobile in all the area through positioning information of a GPS positioning system on each electric automobile. In a position relation function calculating method of the movable charger and the electric automobile, a local optimal greedy algorithm is used to solve a real-time optimal ordered charging scheme so as to realize a user interaction characteristic. The station-level management server carries out data excavation prediction on user data and power grid data and carries out performance analysis on a storage battery so that a user individuation demand is satisfied under the condition that cell losses are less and effective optimization to a power grid is realized.

A method for adaptively partitioning a buffer in a shared buffer switch is provided. The buffer partitioning method for a shared buffer switch which has a plurality of input ports, a plurality of output ports, and a shared buffer, the method for determining whether or not to store a cell, which is newly received through one of the input ports, in the shared buffer comprises the steps of (a) determining a buffer area of the shared buffer in which the newly received cell is stored; (b) determining a cell discard threshold with respect to the total number of cells stored in the shared buffer and the changing rate, with respect to time, of the total number of the cells; and (c) determining whether or not to store the newly received cell in the shared buffer, by comparing the number of cells stored in the buffer area in which the newly received cell is to be stored, with the cell discard threshold. In the method, using a cell discard threshold which is determined with respect to the total number of cells stored in a shared buffer and the changing rate of the total number of cells, it is determined whether or not to store a newly received cell. Therefore, the shared buffer switch adaptively handles changes in inflowing traffic volume and changes in outflowing cell traffic volume such that cell loss due to cell discard is effectively prevented.

The present invention discloses a business intercommunication test method and device for asynchronous transferring mode exchange. Test business flow is made to traverse all exchange channels, and theATM test card compares the final business flow with the initial business flow, counts and judges the cell loss and error data, and reports the test result. The present invention solves the problem ofdata source in high-speed cell business flow test and can perform the high-load business test of exchanger. The business flow produced by the test device may be exchanged to all channels without testleakage. The present invention may be used in high-efficiency automatic test.

The present invention generally relates to use of a stable solid pharmaceutical compositions that includes a clavulanate as the pharmaceutically active ingredients in an immediate-release or an extended-release solid dosage form. The composition can be used in a method of treating a neurodegenerative disease, providing neuroprotection, or preventing neuronal cell loss or death. Exemplary neurodegenerative diseases include Parkinson's disease, Alzheimer's disease and multiple sclerosis.

The invention discloses a preparation method of a liquid-based cell. The preparation method comprises the following steps: step 1, taking a cell smearing device, wherein the cell smearing device comprises a tray, a preparation warehouse fixed on the tray, a glass slide positioned on the lower end surface of the preparation warehouse and used for plugging the lower end of the preparation warehouse, a filter membrane detachably arranged in the preparation warehouse and arranged in the circumferential direction of the preparation warehouse, a filter chamber formed in the preparation warehouse and positioned above the filter membrane and a cleaning chamber formed in the preparation warehouse and positioned under the filter membrane; adding cleaning liquid into the preparation warehouse and enabling all the cleaning liquid to enter the cleaning chamber and the liquid level of the cleaning liquid to be flush with the filer membrane; step 2, taking and adding a mixture of a sample and a preservation solution into the filter chamber, enabling the mixture to pass through the filter membrane to enter the cleaning chamber and enabling a cell to settle to the bottom of the cleaning chamber; step 3, removing the filter membrane as well as impurities on the filter membrane and enabling natural settlement to remove liquid supernatant; adding cleaning liquid, centrifuging and abandoning liquid supernatant; step 4, extracting the glass slide, fixing, dying and sealing. The preparation method has the effect of reducing the cell loss.

The present invention relates to inhibitors of BAG and uses thereof, such as to inhibit cell loss, inhibit parkin sequestration, and/or inhibit formation of protein aggregates. More specifically, the present invention relates to BAG inhibitors used to inhibit neurodegeneration and to treat a neurodegenerative disease, such as Parkinson's disease.

A method for measuring blood levels of β cell DNA that is released upon β cell death by using a quantitative probe technology to detect amplified methylated and demethylated forms of the insulin gene DNA, representing normal tissue and β cell specific origin, respectively. Using probes permits the sensitive and specific identification of demethylated insulin DNA patterns that are present only in β cells. The method offers a bioassay for detecting β cell loss in diabetes, useful for screening of prediabetes, monitoring of disease progression, and selection and monitoring of therapies. The technique finds potential use in both Type I and Type II diabetes, as well as gestational diabetes.

The invention provides an air-cooled battery pack with high heat dissipation efficiency. A second side plate can be moved along a first mounting plate so that the opening and plugging of a first heat emission hole on the first side plate are realized. A second sealing plate can be moved along a second mounting plate so that the opening and plugging of a second heat emission hole on the second sealing plate are realized. The air-cooled battery pack ensures the heat dissipation of the battery pack and the tightness of the battery pack. Through arrangement of fans along four directions of a cell, cross-ventilation is improved and fast heat dissipation is realized. Multiple cell support units are flexibly connected. Pressure relief sheets are arranged. The pressure relief sheets between each two adjacent cell support units are arranged at an interval. In cell explosion, heat and pressure can be effectively released, the explosion-caused surrounding cell loss is reduced, the safety coefficients of the module, assembly and whole battery pack are improved, a battery pack maintenance cost is reduced, a contact area with the air is increased and a part of heat produced by cell work is released.

The invention relates to a method for preparing grease rich in DHA and special equipment therefor. The method comprises the following steps: in an aeration fermentation tank containing a fermentation culture medium, cultivating schizochytrium CGMCCNo.7693 in a mechanical stirring manner; and extracting and recovering the grease rich in DHA from schizochytrium cells, wherein the culture process comprises continuous fed-batch cultivation of a medium, sedimentation-adverse perfusion of cells, and periodical discharge of fermentation liquor; the special cell sedimentation tank comprises a primary sedimentation tank, a secondary sedimentation tank, seal covers, openings and outlets; the primary sedimentation tank and the secondary sedimentation tank are arranged at an interval and are of conical shapes in appearance; the seal covers are arranged on the primary sedimentation tank and the secondary sedimentation tank; the openings are formed in the seal covers; the outlets are formed in the bottoms of the primary sedimentation tank and the secondary sedimentation tank; a liquid inlet pipe is arranged on the primary sedimentation tank; a supernate discharge pipe is arranged at the upper part of the secondary sedimentation tank; the primary sedimentation tank and the secondary sedimentation tank are connected through a communicating pipe; and the outlets are connected with a fermentation tank through concentrated cell reflux pumps and pipes. According to the method and the special cell sedimentation tank, the problems in a micro algae continuous cultivation technique that cells are difficultly intercepted and cell loss is serious are solved, the special cell sedimentation tank has the advantages of reduction of manual operation, easiness in automatic production, short production cycle and the like.

The present invention relates to methods for modulating a β-cell population using a TM4SF4 modulator or a modulator of a TM4SF4 homolog. More particularly, the invention relates, inter alia, to methods and compositions for generating, expanding, and maintaining a β-cell population and treatment of diseases associated with the loss of β-cells using a TM4SF4 modulator or a modulator of a TM4SF4 homolog.

The invention relates to a biologic patch material and concretely relates to a biologic patch material for cardiac repair. The biologic patch material comprises (i) at least two porous structural layers that are compounded together, wherein the porous structural layer comprises a first structural layer for preventing repairing cell loss and a second structural layer for loading (or accommodating) cardiac repairing cells and the repairing cells are selected from cardiac stem cells, cardiac progenitor (precursor) cells or their composition (derived from embryonic stem cells, induced pluripotent stem cells, heart tissue stem cells, bone marrow and peripheral blood), and (ii) repairing cells loaded on the second structural layer, wherein at least a part of or all the repairing cells are located in gaps of the second structural layer. The biologic patch material can load growing of repairing cells and has very good biocompatibility and mechanical strength.

The invention provides a method for optimizing charge and discharge power of an electric car charge-storage-discharge integrated power station. The method is characterized in that the interaction power between an integrated power station and a power grid is optimized through a particle algorithm, so that the difference between peak and valley for the power grid load can be greatly reduced; and the capability that the integrated power station participates in the power grid auxiliary service has positive correlation with the capacity of a power station energy storage system, that is, the greater the capacity becomes, the greater the capability that the integrated power station participates in the power grid auxiliary service becomes, and the more significant the effect of peak clipping and valley filling becomes. The method for optimizing charge and discharge power of an electric car charge-storage-discharge integrated power station can also further reduce the cell loss cost for the charge-storage-discharge integrated power station.

The invention discloses a method for improving proliferation ability and performance of umbilical cord blood OECs (outgrowth endothelial cells). The method comprises the steps as follows: pure umbilical cord blood-derived OECs are obtained by separation from fresh umbilical cord blood and purification, and mixed with umbilical cord-derived mesenchymal stem cells for culture, and a culture medium is a EGM-2BulletKit complete medium containing the fetal bovine serum with the volume fraction of 10%. By the aid of the method, the proliferation efficiency and the vascularization ability of the OECs are improved. After the method is applied, due to the immune down-regulatory effect of the mesenchymal stem cells and the expansion of the total treatment cell amount, the cell loss proportion caused by immune reactions is reduced, the treatment effect is guaranteed, and the application possibility of the umbilical cord blood-derived OECs is increased. The mesenchymal stem cells also have the ability of assisting in maintaining blood vessels, so that the long-term treatment effect is guaranteed. The method is mainly applied to blood vessel repair treatment, tissue engineering and the like.

The invention discloses a micro-immunofluorescence detection method without cell loss. The micro-immunofluorescence detection method comprises the steps of: performing 0.22 um Spin-X Centrifuge Tube sterilization; transferring cells into a sterilized 0.22 um Spin-X Centrifuge Tube for carrying out subsequent steps directly or carrying out subsequent steps fter cultivation; and performing the subsequent steps of PBS buffer solution rinsing, 4% paraformaldehyde fixation, methanol treatment on ice, 0.3 M glycine confining liquid treatment, 4% BSA confining liquid treatment, fluorescence primary antibody room temperature dark incubation, staining in DAPI staining solution, slide sealing after placing a filtering membrane in a slide, and observation of the slide under a laser scanning cofocal microscope. The method makes up for the shortcoming that the immunofluorescence technique is difficult to achieve precise screening of specific cells, and provides a simple, precise and visual technique for screening specific cells in a mixed cell population.

A method and apparatus for embedding cells that utilizes a flow-through embedding technique maximizes the efficiency of extractions and decreases time for embedding the cell fragments, minimizes cell loss, and automatically positions cell samples at the position in which a microtome blade will section them. The apparatus includes a cell flow pathway defined by an inflow tube for delivering cell fragments from a cell sample to a sample port. The sample port is in fluid communication with a tissue cassette having attached thereto a filter. The cell flow pathway is in communication with a reagent flow pathway for delivering the reagents through the sample port to the cassette. The apparatus is configured such that the application of pressure directs the cell fragments from the cell sample through the cell flow pathway, and effects delivery of the reagents through the reagent flow pathway. The apparatus produces an embedded cell block having concentrated cells near the plane of the block to be sectioned in a quick and efficient manner.