16 results about "Cell loss" patented technology

The utility model discloses a microfluidic system for target object pairing, which comprises a first flow channel, a second flow channel, an oil liquid flow channel, a buffer liquid flow channel, a matching flow channel and a pairing micro valve, a first micro valve, a second micro valve and a buffer liquid micro valve are arranged, the matching flow channel is communicated to the oil liquid flow channel, and the first flow channel, the second flow channel and the buffer liquid flow channel are all communicated to the matching flow channel; when a single first target object and a single second target object exist in the matching runner, the first micro valve and the second micro valve are in a closed state, and the matching micro valve and the buffer solution micro valve are in an open state; and the buffer liquid in the buffer liquid flow channel feeds the single first target object and the single second target object in the flow channel into the oil liquid in the oil liquid flow channel to form liquid drops. According to the utility model, other first target objects and / or other second target objects can be completely prevented from entering liquid drops, so that the success rate of packaging is improved, and the cell loss caused by multiple packaging is reduced.

The invention discloses a microfluidic system for target object pairing and a pairing method, the microfluidic system comprises a first flow channel, a second flow channel, an oil liquid flow channel, a buffer liquid flow channel, a pairing micro valve, a first micro valve, a second micro valve and a buffer liquid micro valve, the first flow channel, the second flow channel and the buffer solution flow channel are all communicated to the matching flow channel; when a single first target object and a single second target object exist in the matching runner, the first micro valve and the second micro valve are in a closed state, and the matching micro valve and the buffer solution micro valve are in an open state; and the buffer liquid in the buffer liquid flow channel feeds the single first target object and the single second target object in the flow channel into the oil liquid in the oil liquid flow channel to form liquid drops. Other first target objects and / or other second target objects can be completely prevented from entering the liquid drops, the packaging success rate can be improved, and cell loss caused by multiple packaging is reduced.

The utility model provides a multi-stage filter tube for pretreatment of a cell suspension, which is characterized in that each second single tube body serves as a middle tube body and is of a transparent cylinder structure, an external thread structure is arranged at the port of the top end of each second single tube body, and an internal thread structure matched with the external thread structure is arranged at the port of the bottom end of each second single tube body; every two adjacent single pipe bodies can be connected and installed through matched thread structures, and connecting pipe bodies which are sequentially arranged from top to bottom and provided with multiple stages of filtering hole diameters which are reduced step by step are formed. The first single tube body is used as the uppermost tube body; the third single pipe body is used as the lowermost pipe body; and the air pressure adjusting device is communicated with the first single pipe body. According to the utility model, through a multi-stage filtering structure, the aperture is reduced stage by stage, so that the filtering efficiency is improved, and the cell loss and the impurity residue are obviously reduced; the cell integrity is ensured by adopting lower pressure, the damage to the cells caused by overhigh impact force is effectively avoided, and meanwhile, the impurities are ensured to be fully removed.

The utility model discloses an on-ice operation experiment table, and relates to the field of test equipment. The on-ice operation experiment table comprises a working table and further comprises an experiment groove formed in the working table, sliding grooves are symmetrically formed in the working table, and the two sliding grooves are located on the two sides of the experiment groove respectively; the sliding block is connected into the sliding groove in a sliding mode; the first spring is arranged in the sliding groove; the two first clamping plates are attached to the outer walls of the two sides of the ice block respectively, so that the ice block is clamped and fixed, the stability of the ice block in the experiment groove is kept, meanwhile, the second clamping plates located at the two ends of the first clamping plates are attached to the two ends of the ice block under the pushing of the second compressed springs, and the ice block is clamped and fixed. The stability of ice blocks in the experimental tank is improved, and the situation that the ice blocks gradually melt along with time and displace in the experimental tank, so that in the operation process of cell cryopreservation and resuscitation, a cell container topples over, and cell loss is caused is avoided.

The invention belongs to the technical field of cell smears, and discloses a cell filtering, sorting and enriching smear device which comprises an accommodating and resuspending mechanism, a filtering mechanism and a smear mechanism. The accommodating and resuspending mechanism comprises a sample accommodating assembly and a resuspending assembly, the sample accommodating assembly and the resuspending assembly can be coaxially and integrally connected or mutually independent, the sample accommodating assembly accommodates a liquid biological sample, and the resuspending assembly accommodates resuspending liquid; the filtering mechanism is detachably connected to the sample containing assembly and further detachably connected to the resuspension assembly, a filtering membrane is arranged in the filtering mechanism, and a liquid biological sample is filtered and enriched through the filtering membrane and then is resuspended to obtain a cell suspension; the smear mechanism is connected to the centrifugal smear machine and can bear a glass slide and contain the filtering mechanism, and the cell suspension can flow to the glass slide from the filtering mechanism. The cell filtering, sorting, enriching and smearing device can realize cell separation, enrichment and smearing processes, prevent cell loss, improve the smearing quality and shorten the smearing time.

The application belongs to the field of cell therapy process engineering, and discloses a CAR-NK cell high-yield culture method with dynamic process control. The method realizes the dynamic balance of nutrient supply and shear force in the culture system by introducing the strategy of combining culture stage regulation with one-time large-volume liquid replacement in a swing bioreactor. The method realizes online interception and rapid replacement of the culture medium in the high-density cell stage by using the built-in interception membrane of the reactor culture bag, thereby avoiding cell loss and nutrient depletion. Meanwhile, the method effectively maintains the CAR expression level and cell functional activity by cooperating with the optimized culture medium formula and the application of a transfection aid. The method has the advantages of simple operation and high repeatability, is suitable for various swing or closed reactor platforms, has good amplification performance and industrial application value, and can provide an efficient and stable technical scheme for the preclinical preparation and industrial production of CAR-NK cell immunotherapy.

The invention discloses an absolute quantitative detection method for urine exfoliated podocyte, relates to the technical field of biomedical detection, and aims to solve the problem that the urine exfoliated podocyte cannot be accurately and absolutely quantified due to uncertain cell loss in a sample treatment process in the prior art. The method comprises the following steps: before carrying out any physical treatment on a urine sample, adding a known number of internal standard reference substances with physical characteristics similar to those of cells into the urine sample, carrying out co-enrichment treatment on a mixed sample containing target podocytes and the internal standard reference substances, and preparing a cell slide by adopting a standardized slide preparation technology; and carrying out podocyte specific immunofluorescence staining on the slide. Through the design of internal standard preposition and whole-course synchronous calibration, the cell loss error in the operation process is effectively overcome, the traditional semi-quantitative detection is improved into accurate and repeatable absolute quantification, and the reliability and clinical application value of a detection result are remarkably improved.

The invention relates to the technical field of blood routine examination, and discloses a condensation element removing device and method, and the condensation element removing device comprises a first container, a second container, a filter screen and a carrier. The blood collection tube, the first container and the second container are assembled and uniformly mixed up and down, a detection sample moves back and forth in the blood collection tube and the second container, and when the detection sample flows to the second container from the blood collection tube and the first container, blood cells are intercepted by the filter screen, and blood plasma without the blood cells enters the second container, so that the blood collection tube and the second container are separated from each other. And combining condensed elements in the filtered plasma with an anti-human IgM antibody on the surface of the carrier, fixing the condensed elements on the surface of the carrier, pouring the reacted plasma into a first container and a blood collection tube, fully and uniformly mixing the reacted plasma with blood cells, and carrying out clinical blood routine examination. Theoretically, the process does not cause cell loss, red blood cells do not agglutinate again due to temperature change, and the method is more favorable for helping clinical laboratories to solve the problems of tedious treatment, long time consumption and the like of condensation set samples.

Disclosed in the present invention are a microfluidic system for target object pairing, and a pairing method. The microfluidic system comprises a first flow channel, a second flow channel, an oil liquid flow channel, a buffer solution flow channel, a pairing flow channel, a pairing microvalve, a first microvalve, a second microvalve and a buffer solution microvalve; the pairing flow channel is communicated with the oil liquid flow channel; the first flow channel, the second flow channel and the buffer solution flow channel are all communicated with the pairing flow channel; and when a single first target object and a single second target object are present in the pairing flow channel, the first microvalve and the second microvalve are in a closed state, and the pairing microvalve and the buffer solution microvalve are in an open state, so that a buffer solution in the buffer solution flow channel delivers the single first target object and the single second target object in the pairing flow channel into an oil liquid in the oil liquid flow channel to form liquid drops. The present invention can completely prevent another first target object and / or another second target object from entering the liquid drops, thereby facilitating the improvement of the success rate of packaging, and reducing cell loss caused by multi-packaging.

The present invention provides a negative pressure balanced centrifuge bottle, comprising: a centrifuge bottle body and a small opening with an inner diameter of 1 to 3 mm; the small opening is connected to the centrifuge bottle body via a conical body, and a thin tube is disposed between the small opening and the centrifuge bottle body. The negative pressure balanced centrifuge bottle provided by the present invention is installed in a centrifuge, and the centrifuge can effectively complete the entire process of separating cell specimens, concentrating cells, and preparing sections by repeatedly running the centrifuge at high and low speeds. This shortens the preparation time, greatly saves time, reduces workload, and achieves high preparation efficiency. It also effectively avoids the problem of cell loss caused by multiple transfers of cell specimens.

The low-speed horizontal centrifugal machine comprises a machine body, a centrifugal support is in transmission connection with an inner cavity of the machine body, a plurality of clamping units are fixedly connected to the peripheral side of the centrifugal support, a centrifugal tube is clamped between every two adjacent clamping units, a protrusion is arranged at the bottom end of each centrifugal tube in an abutting mode, and a tray is fixedly connected to the bottom face of each protrusion. The tray is fixedly connected with the centrifugal bracket; the clamping unit comprises a fixing assembly and a clamping assembly, the clamping assembly comprises a first pipe clamp and a second pipe clamp, one end of the first pipe clamp is hinged to one end of the second pipe clamp through a pin shaft, the other end of the first pipe clamp and the other end of the second pipe clamp are fixedly connected with mounting plates respectively, and the two mounting plates are in threaded connection with each other; the outer side of the first pipe clamp and the outer side of the second pipe clamp are fixedly connected with supporting rods correspondingly. According to the utility model, the centrifugal capacity is increased to realize less-time or one-time centrifugal collection, so that the urine sample cell collection efficiency can be effectively improved, the cell loss is avoided, and the cell collection quantity is increased.

The utility model discloses a flow type tube with a cell enrichment device. Relates to the technical field of cell enrichment and comprises a flow tube body, a top cover is arranged at the top of the flow tube body, an opening is formed in the bottom of the flow tube body, and a filter membrane is arranged at the opening and completely covers the opening. The top of the bottom cover is connected with the opening of the flow type pipe body, a through hole is formed in the side wall of the bottom cover, a protective cover is arranged at the through hole, and the protective cover is hinged to the side wall of the bottom cover; the negative pressure vacuum pump is arranged to generate negative pressure in the bottom cover, liquid in the flow type pipe body is affected by the negative pressure and is sucked into the bottom cover, cells are intercepted in the flow type pipe body through the filter membrane, and the cells are separated from the bottom cover through the negative pressure vacuum pump. And the problem of cell loss caused by a traditional centrifugal cell enrichment method is avoided.

The invention provides a cell freezing and embedding method for immunohistochemical staining, and belongs to the technical field of biology. The cell freezing and embedding method comprises the following steps: collecting cells; adding an embedding medium into the cells, and uniformly mixing the cells with the embedding medium; uniformly mixing the cells with an embedding medium, and freezing in liquid nitrogen; after the embedding medium is solidified, taking out the pre-embedded block; and carrying out secondary embedding on the pre-embedded block by using an embedding medium to obtain the cell frozen block for immunohistochemical staining. The cell freezing and embedding method disclosed by the invention is low in cell loss, low in cost and simple to operate; and the obtained frozen block slice is low in difficulty, is beneficial to long-term preservation and reuse, is better in quality and good in antigen preservation effect when being used for immunohistochemical staining, avoids false negative, and has a good application prospect.

The application discloses a method for preparing a cell block by using mucin emulsifier and application thereof, and the preparation method comprises the following steps: centrifuging a non-gynecological sample to obtain a first separation liquid, adding the first separation liquid into a formaldehyde-alcohol solution to centrifuge to obtain a second separation liquid, and adding the second separation liquid into mucin emulsifier to centrifuge to obtain a cell block. The preparation method is simple, the manufacturing cost is low, the cell block has high enrichment efficiency, cell loss is small, and the mucin emulsifier does not affect cell activity. The cell block prepared by using the mucin emulsifier has the following advantages: the appearance of the section of the cell block is complete, the section has a regular circular structure, the porosity is moderate, and the thickness is uniform; the application effect of the section in two kinds of staining is good: the hematoxylin-eosin staining has a clear background and clear contrast, cells are uniformly distributed in a circular area, cell morphology presents a stereoscopic effect, and the excellent rate of the stained section is 98.94%; the immunohistochemical staining has a clean background, and structures and cells are specifically dyed, and the excellent rate of the stained section is 99.30%, so that the cell block has a wide application prospect.

The invention discloses a centrifugation and dyeing all-in-one machine, and belongs to the technical field of biological experiment equipment, the centrifugation and dyeing all-in-one machine comprises a workbench, the workbench is provided with a centrifugation module, the centrifugation module comprises a collection box, and a centrifugation frame is rotatably arranged in the collection box; a plurality of tube seats are distributed on the centrifugal frame; supporting shafts are arranged on the two sides of each pipe base, each supporting shaft is rotationally connected to a centrifugal frame, the centrifugal frame is slidably connected with a toothed bar through a first spring, and the toothed bars are connected with the corresponding supporting shafts in an engaged mode. When supernate is poured out, the supernate is poured out from different sides of the centrifugal tube in two times. In the first pouring process, the cells are accumulated towards the inclined side of the centrifugal tube; after the tube seat resets and rotates, supernate is poured out from the other side of the centrifugal tube during secondary pouring, so that the phenomenon that cells are poured out together when the supernate is poured out is avoided, experimental errors caused by cell loss are effectively reduced, and more reliable data support is provided for medical decision making.