206 results about "Histocytochemistry" patented technology

The present disclosure provides isolated antibodies that specifically bind to human PD-L1, as well as antigen binding fragments of such antibodies, and kits comprising the anti-PD-L1 antibodies or binding fragments and a set of reagents for detecting a complex of the antibody, or antigen binding fragment thereof, bound to human PD-L1. The antibodies and antigen binding fragments of this disclosure are useful for immunohistochemical detection of human PD-L1 expression in tissue samples. Nucleic acid molecules encoding the antibodies and antigen binding fragments of this disclosure, as well as expression vectors and host cells for expression thereof, are also provided.

Scanning and analysis of cytology and histology samples uses a flatbed scanner to capture images of the structures of interest such as tumor cells in a manner that results in sufficient image resolution to allow for the analysis of such common pathology staining techniques as ICC (immunocytochemistry), IHC (immunohistochemistry) or in situ hybridization. Very large volumes of such material are scanned in order to identify cells or clusters of cells which are positive or warrant more detailed examination, and if analysis at higher resolution is necessary, information regarding these positive events is transferred to a secondary microscope, such as a conventional scanning microscope, to allow further analysis and review of the selected regions of the slide containing the sample.

The present invention describes a method utilizing a set of genes or gene products whose altered expression in cancer tissue, particularly head and neck cancer and other carcinomas, or its adjacent normal tissues predicts (a) probability of recurrence in time after treatment (b) sensitivity or resistance to therapies or (c) probability of metastasis at the time of initial discovery of the tumor. Furthermore, the invention describes methods of determining the molecular signature in tumor tissues, tissues adjacent to the tumor, or in saliva by using DNA microarray techniques, quantitative real-time PCR, immunohistochemistry or other methods that are used for determining gene or gene product expression levels.

Methods and apparatuses for gently removing embedding media from biological samples at temperatures below the embedding medium melting point with liquid composition using batch methods or automated instruments prior to immunohistochemical (IHC), in situ hybridization (ISH) or other special staining or histochemical or cytochemical manipulations.

A method of in situ immunohistochemical analysis of a biological sample is provided. The method allows for the multiplex and simultaneous detection of multiple antigens, including multiple nuclear antigens, in a tissue sample.

The invention discloses a preparation method for a gill tissue paraffin section. The preparation method comprises the following steps: fixing, decalcifying, dehydrating, transparentizing, carrying out paraffin permeation, embedding, slicing, sticking sections, expanding the sections, de-waxing and rehydrating, staining, re-staining, sealing and the like. Compared with an existing paraffin section manufacturing method, an operation process of dehydrating, transparentizing and immersing by wax is improved; the preparation fixing and tissue wax immersing effects of gill tissue paraffin are improved; a slicing problem when the gill tissue paraffin section is prepared is improved; the structure definition of the gill tissue section is greatly improved; a plurality of problems in a gill tissue manufacturing process in the prior art are solved. The preparation method is good for antigen positioning of immunocytochemical staining, so that when experiments including in-situ hybridization, immunohistochemistry, immunofluorescence and the like are carried out on the gill tissue paraffin section, tissue distribution and cell positioning of some genes and proteins can be displayed, and further feasible conditions are provided for carrying out gill research on levels of cells, genes and proteins.

The invention relates to a building method of a zebra fish hyperlipidemia model and application of the animal model to hyperlipidemia disease research and lipid-lowering drug screening. The building method of the zebra fish hyperlipidemia model mainly includes the steps of zebra fish selection, feeding of zebra fish by yolk powder, histochemical staining or fluorescent staining, image analysis and/or microwell plate analysis and statistical analysis. The building method has the advantages of simplicity, convenience, rapidity, economy, high efficiency, high throughput and the like, and the model can be used for hyperlipidemia disease research and lipid-lowering drug screening. The building method and application of the zebra fish bacterial infection model are of great significance to acceleration of research and development on lipid-lowering drugs and improvement on treatment of patients suffering from hyperlipidemia.

Relating to methods able to conduct fast histochemistry and other analyses in plant tissues, the invention specifically relates to an embedding medium and a direct frozen section method. The embedding medium is composed of polyvinyl alcohol, polyethylene glycol, carboxymethylcellulose sodium, glycerol, formaldehyde, collagen, plant gel, and deionized water. According to the frozen section method, a plant material is not fixed, and a sample subjected to histochemical staining and other treatments or a fresh tissue is wrapped on a sample table directly to perform frozen section. The method solves the problem that in the microscopic observation of phytohistochemistry, immunolocalization, in-situ hybridization and other studies, a target chemical substance is easy to dissolve in a fixation agent, and is impossible to be fixed first and then sectioned, or the target chemical substance dissolves in an alcohol substance and is impossible to be dehydrated by ethanol.

The invention discloses a preparation method and an application of a tissue slice for observing temporal-spatial distribution of early embryo development in vivo. The preparation method comprises the following steps that 4% paraformaldehyde fixing, upward gradient ethanol dehydration, wax dipping, embedding, serial section, baking, dewaxing and downward gradient ethanol rehydration are performed in sequence on oviducts or uterine tissues which contain mice embryos in every period, and finally, after haematoxylin-eosin staining is performed on the tissues in the slice, neutral gum is used for sealing the slice, or after immunofluorescence histochemical staining is performed on the slice, a fluorescence resistant quenching sealing agent is used for sealing the slice. The tissue slice disclosed by the invention can be used for manufacturing a map of early mice embryo development and detecting the expression of Crb3 in the mice embryos in every period of development in vivo. The preparation method has the advantages that positions of all organs in the embryos can be relatively fixed, so that the position change of embryo cells in a genital tract and the continuity of embryo development can be conveniently observed, the structure is clear, and the tissue slice is convenient to store.

The invention discloses a double-stained kit for auxiliary diagnosis of benign or malignant hepatocellular tumor and application thereof. The kit comprises the following components: mixed primary antibody of Arginase-1 and Glypian-3, mixed second antibody of alkaline phosphatase conjugated goat anti-rabbit second antibody and horse radish peroxidase labeled goat anti -mouse second antibody, chromogenic reagent AP-Red and DAB (Diaminobenzidine), and TBS (Tris buffer saline) buffer solution. Two immunohistochemical labels provided by the invention are used for auxiliary diagnosis of benign or malignant hepatocellular tumor and differentiation degrees of tumor, the two labels are displayed through staining label by different detection systems and utilizing the differences of target cells stained by each label, information quantity and differential staining on the same radiograph are improved, readability and accuracy of radiograph reading are increased, so that the double-stained kit has important significance to identification of benign or malignant hepatocellular tumor and differentiation degrees of tumor.

The present invention provides compositions, kits, assembles of articles and methodology for carrying out processes that permit biological enzymes to act directly on metals and metal particles. In particular, the invention relates to use of enzymes to selectively deposit metal to the vicinity of a target molecule. The invention also relates to linking of metals to enzyme substrates, control of enzymatic metal deposition and applications of enzymatic metal deposition to sensitively and selectively detect target molecules such as biomarkers in various biological samples, such as chromogenic immunohistochemical (IHC) detection in situ by using bright field light microscope.

The invention discloses a polypeptide that can be specifically combined with lung cancer cells and a preparation method and application thereof. The polypeptide specifically combined with lung cancer cells shows peptide library reducing screening by a bacterial virus so as to obtain bacterial virus clone specifically with lung cancer; the bacterial virus clone having stronger affinity with lung cancer is identified by Elisa and an immunohistochemical method; and sequencing is sent so as to obtain a 12 polypeptide, and the amino acid sequence is shown as SEQ ID NO: 1. The 12 polypeptide screened by the invention not only has the advantages of traditional antibody molecule but also has small molecular weight, strong penetrating power and easy arriving to tumor tissue; the 12 polypeptide has good tumor targeting effect and more sensibility of tumor diagnosis, and can be used for preparing a kit for tumor diagnosis and drug for curing tumors.

The present invention relates to a method for enhancing immunoreactivity of a tissue or cell sample fixed in a fixing medium, a target retrieval composition and its use. The method comprises providing a carrier in a horizontal position, said carrier having thereon a tissue or cell sample, said tissue or cell sample being on top of the carrier; contacting substantially the tissue or cell sample side of the carrier with a buffered target retrieval solution, wherein the target retrieval solution remains otherwise exposed to the environment; heating the tissue or cell sample and the target retrieval solution to a temperature above 100° C. The invention furthermore concerns the automated immunohistochemical staining of said samples, in particular the reactivation of antigens masked by fixation.

A system and method for Raman spectral analysis for diagnostic purposes to identify the disease state of biological tissue. The sampled tissue can be any human or animal tissue, including but not limited to prostate, breast, cervical tissue, etc. Raman spectroscopy is used to provide Raman bands which are decomposed and their chemical fingerprints identified after mathematical modeling. The method enables identification of chemical compounds in the tissue being diagnosed. Comparison with reference samples permits inferences concerning the presence or absence of disease, the stage of disease, and/or the response of disease to treatment. The method provides sufficient information about the chemical composition of the tissue to differentiate between normal and abnormal states, and may also reveal the locations of abnormalities. The method may also be used to monitor the treatment of a subject and/or the response of disease to drugs or other treatments.

The present invention provides a rapid frozen section method for camphor leaf. The method is as below: fixing fresh cut camphor leaf at 4 DEG C for 2-3 h; maintaining the temperature of box and freezing station at -20 DEG C; evenly smearing a material sample with an OCT frozen section embedding agent to embed the sample deeply in the agent; rapidly cooling and fixing the sample on a sample stage; shaking a rotating lever outside the machine body, and trimming to an observation site; then taking the sample stage along with the embedded block out of a slicer; placing at room temperature for 20-30 s to soften the embedded block; quickly placing the embedded block into the a slicer, and slicing in 15-20 s, wherein the slice thickness is 10-20 mum; gently touching the embedding agent around the section by using a dissecting needle, so as to attach the section to the dissecting needle; and transferring the section to a slide glass outside the slicer by the dissecting needle and fully expanding the section. Fully expanded section generally does not need special treatment for direct dyeing or tissue chemical analysis and observation and photographing under the microscope.

The invention discloses a tumor necrosis factor-alpha induced protein 8 L3 (TIPE3) immunohistochemistry detection kit for diagnosing lung cancer. Contents of the kit comprise a reagent which is used for inactivating endogenous peroxidase and biotin in a human tissue section, a non-specific protein blocking agent which is used for blocking non-specific staining of cross protein reaction in the human tissue section, a rabbit anti-human initial antibody which is combined with human tissue antigen, an antibody diluent, a goat anti-rabbit monoclonal connection antibody which can be connected with the initial antibody and is marked by horse radish peroxidase, a developing reagent which can be reacted with the horse radish peroxidase and used for developing, and a reagent which can specifically stain nuclei in tissues. TIPE3 is positively expressed in various kinds of pathological type lung cancer, tumors can be early detected and found, the kit is high in specificity and high in sensitivity and can be applied to early diagnosis of the lung cancer, a patient can be effectively and timely treated, unnecessary medical cost is reduced, the survival quality of the patient is improved, survival time is prolonged, and the survival rate of the patient who suffers from the lung cancer is improved.

The invention discloses a system used for predicting prognosis of a liver cancer patient. The system comprises a system for detecting expression quantities of four proteins c-Met, Caspase8, CXCR4 andP-P21, and a protein expression quantity data processing system. The system for detecting the expression quantities of the four proteins measures the expression quantities of the proteins through an immunohistochemistry staining method; and the protein expression quantity data processing system converts the expression quantities of the four proteins in a para-carcinoma tissue of the to-be-predicted liver cancer patient into prognosis score values, and predicts the prognosis of the liver cancer patient according to the prognosis score values.

The invention discloses a Ki67 cell nucleus counting method and system based on pathological immunohistochemistry, and the method comprises the steps: placing a pathological section after Ki67 staining under an optical microscope, and collecting an image of the pathological section after Ki67 staining through visual detection equipment CCD; obtaining a Ki67 negative cell RGB image and a Ki67 positive cell RGB image; carrying out intercellular substance interference filtering on the RGB image to generate a binary image of Ki67 negative cells and a binary image of Ki67 positive cell RGB; carrying out element marking on the binary image, and calculating the number and indexes of connected regions of the binary image; calculating the area of each connected region, taking a standard cell connected region as a reference form, and filtering the connected region with the area smaller than the reference form; segmenting the adherent cells according to the standard that the area of the connectedregion is equal to twice of the reference form; marking Ki67 positive cells and Ki67 negative cells according to the index of the final connected region, and performing counting.

The invention discloses a gene relevant to low-temperature, drought, ABA and high salt resistance of wheat and application thereof, and relates to the technical field of biology. The invention provides expression mode analysis of the wheat TaCOBL gene. The result shows that the gene realizes the up-regulated expression through low-temperature induction and realizes down-regulated expression through drought, ABA and high salt induction. A promoter-GUS fusion expression double-ingredient vector is respectively built; according to the GUS active determination and tissue chemical dyeing result, under the low-temperature induction effect, the promoter of the gene can greatly improve the GUS gene expression level; the result shows that the promoter of the gene belongs to the induction type promoter; under the low-temperature induction, the activity of the Hap5B-a type promoter is higher than that of an Hap5B-b type promoter. The result proves that the activity of the promoter can be enhancedthrough the insertion of promoter region TATA four basic groups. The gene type Hap5B-a is an excellent allelic variation.

The present invention relates to a method of biological labeling that occurs via a free radical chain reaction. The labeling occurs due to deposition of a detectable reporter molecule from a media comprising a substance comprising at least two moieties of a peroxidase enzyme substrate (termed herein ‘cross-linker’) in a target site comprising peroxidase activity and a biological marker. The labeling reaction described herein may generally be used to detect targets in a host of experimental schemes for detecting and visualizing a biological or chemical target, including immunohistochemistry (IHC), in situ hybridization (ISH), antibody-based staining methods such as ELISA, Southern, Northern, and Western blotting, and others.

The invention discloses a system for predicting prognosis of patients with lung adenocarcinoma and judging benefit of an adjuvant chemotherapy. The system comprises a system used for detecting expression quantities of six proteins, i.e., c-Src, Cyclin E1, TTF1, p65, CHK1 and JNK1, and a protein expression quantity data processing system. The system used for detecting the expression quantities of the six proteins can determine the expression quantities of the proteins by using an immunohistochemistry staining method; the protein expression quantity data processing system converts the expression quantities of the six proteins in lung adenocarcinoma tissues, separated from to-be-predicted patients with the lung adenocarcinoma, into a prognosis score, and predicting the prognosis of the to-be-predicted patients with the lung adenocarcinoma according to the prognosis score, and/or predicting whether the to-be-predicted patients with the lung adenocarcinoma are benefited from the adjuvant chemotherapy or not according to the prognosis score.

This invention discloses to a kind of immunofluorescence mark reagent box. It is a reagent box applied for the aspects of immunity mark which uses the green fluorescence protein (GFP) as the indicator, the staphylococcus protein A (SPA) as the antibody. It also discloses the preparation method of the reagent. This invention can applied wide range in the field such as the immunity fluorescence mark, immunity histochemistry, biochemistry analysis, antibody test, protein chip and so on. It can replace the exist product efficiently, its operation is more simple, stability, delicacy and reliability, it is especially the same with the high flux analysis which analyze by the fluorescence analysis instrument.

The invention discloses a reagent for auxiliarily diagnosing lung cancer lymph node metastasis, comprising 8 antibodies which are used for detecting 8 protein markers which are MMP1 (matrix metalloproteinase-1), TIMP1 (tissue inhibitor of metalloproteinases metallopeptidase inhibitor 1), IQGAP1 (IQ motif containing GTPase activating protein 1), TPX2 (targeting protein for Xklp2), uPA (Urokinase-type plasminogen activator), Cathepsin-D, Fascin and pIgR/SC (polymeric immunoglobulin receptor/secretory component). By adopting the 8 antibodies and an immunohistochemical staining result, lung cancer lymph node metastasis can be auxiliarily diagnosed, and the reagent is expected to be used for the risk estimation of lung squamous cell cancer lymph node metastasis and the prognostic prediction. The reagent has high creditability, strong practicability and clinical use value based on the clinical routine immunohistochemical staining technology when the reagent is used for auxiliary diagnosis.