Patents
Literature
2 results about "Antigen retrieval" patented technology
Efficacy Topic
Property
Owner
Technical Advancement
Application Domain
Technology Topic
Technology Field Word
Patent Country/Region
Patent Type
Patent Status
Application Year
Inventor
Tissues that have been preserved with formaldehyde, a highly reactive compound, contain a variety of chemical modifications that can reduce the detectability of proteins in biomedical procedures such as immunohistochemistry. Antigen retrieval is an approach to reducing or eliminating these chemical modifications. The two primary methods of antigen retrieval are heat-mediated epitope retrieval (HIER) and proteolytic induced epitope retrieval (PIER).
Multi-histochemical staining method based on controllable staining-decolorizing circulation
The invention relates to the technical field of biomedical detection, and particularly discloses a multiple histochemical staining method based on controllable staining-decolorizing circulation. The method is realized through the steps of water bath-assisted antigen repair, composite pretreatment, alternate labeling dyeing of nanogold and quantum dots, electrochemical-assisted controllable decoloration, dynamic monitoring circulation, optical transparency treatment, multi-modal imaging reconstruction and the like. The key composition comprises a composite decolorizing agent containing a specific electron shuttling medium and a series of coloring agents containing nanogold and quantum dots. The preparation method mainly comprises the following steps: performing incubation reaction on each component and a tissue sample according to a specific sequence, completing controllable decolorization circulation under the assistance of an electric field, and regulating and controlling the process in real time through an optical monitoring system. According to the method disclosed by the invention, the sequential marking and accurate recognition of multiple target molecules on the same tissue sample can be realized; in addition, the preparation method has the advantages of being high in process controllability, good in dyeing cycle stability and high in operation repeatability.
Owner:温伟
TUNEL-tsa multiplex fluorescent staining method based on TSA technology optimization and application thereof
The application belongs to the field of biological detection, and particularly relates to a TUNEL-TSA multiplex fluorescence staining method based on TSA technology optimization and application thereof. Tyramide signal amplification technology (TSA) is used to improve and optimize TUNEL combined immunofluorescence staining. The TSA technology can make the fluorescence signal intensity increase by more than 10 times, while maintaining a more complete cell morphological structure. In the multiplex staining process, the sequential scheme of "antigen retrieval-TSA fluorescence staining-protease K digestion-TUNEL staining" is significantly better than other staining strategies. It provides reliable technical support for establishing a standardized detection system, and has important clinical application and popularization value.
Owner:ZHEJIANG UNIV