12 results about "Antigen retrieval" patented technology

The utility model discloses a container box for immunohistochemical antigen repair, which relates to the technical field of biomedicine and comprises a box body and a sealing cover, the box body is used for accommodating tissue slices and antigen repair liquid, and the sealing cover is connected to a top opening of the box body. A supporting frame used for supporting tissue slices is arranged in a cavity of the box body. The box body, the sealing cover and the supporting frame are all made of materials suitable for ultrasonic conduction. The device is reasonable in layout, compact in structure and convenient to use, the ultrasonic treatment requirement is met through material selection, the antigen repair efficiency and the dyeing quality can be effectively improved, the operation process is simplified, and the experiment cost and the reagent loss are reduced.

To provide a system for unmasking protein antigens and nucleic acid targets from fixed biological samples.SOLUTION: The present disclosure is directed to specimen processing assemblies including (a) a lower plate 10, (b) an upper plate 30 complementary to the lower plate, and (c) a chamber formed therefrom. In some embodiments, the formed chamber is adapted to perform an unmasking operation, e.g., antigen retrieval and / or target retrieval. In some embodiments, the specimen processing assemblies are configured to keep a specimen-bearing substrate 15 horizontal during all processing steps. The present disclosure is also directed to systems including one or more independently operable specimen processing assemblies.SELECTED DRAWING: Figure 14A

The present invention includes compositions and methods for retrieving tumor-related antibodies and antigens. In one aspect, the invention includes a method for Sequential Tumor-related Antibody and antigen Retrieving (STAR) which directly and efficiently identifies potent antibodies that can specifically bind to tumor-related antigens on the tumor cell surface. In another aspect, the invention includes a CAR comprising a nanobody, a transmembrane domain, and an intracellular domain, wherein the nanobody is retrieved by a STAR method. In another aspect, the invention includes a CAR T system that targets CD13 and treats acute myeloid leukemia. In another aspect, the invention includes a CAR T system and ADC that targets CDH17 and treats NETs and other types of tumors expressing this antigen, with tolerable toxicities.

The invention relates to a breast cancer HER2 gene and muscle epithelium marker synchronous detection fluorescence double-staining kit and a method thereof. The double-staining kit comprises a HER2 gene specific fluorescence labeling probe and a CEP17 centromere probe; an anti-CK5 / 6 antibody and a corresponding fluorescently-labeled secondary antibody; the EDTA buffer solution is used for antigen repair; the invention discloses a dyeing buffer solution capable of ensuring double dyeing compatibility. The method is suitable for accurate detection of breast cancer samples with limited infiltration components in a dark field, synchronous analysis of HER2 genes and myoepithelium markers on the same slice is realized, the problems of inaccurate positioning, low efficiency, specimen waste, treatment delay and the like in the existing FISH detection technology are solved, and the method has the advantages of improving the detection accuracy, improving the efficiency, reducing tissue consumption, reducing the cost and the like. And the clinical application potential is improved.

The application provides a tissue staining method based on antigen repair treatment combined with silver staining, comprising the following steps: a) high-temperature heat repair of a tissue section to be treated with an antigen repair solution, and washing with water after cooling to obtain a repaired tissue section; b) silver staining of the repaired tissue section obtained in step a), dehydration, and then mounting to obtain a stained tissue section. Compared with the prior art, the tissue staining method based on antigen repair treatment combined with silver staining provided by the application adopts specific process steps and conditions to realize overall good interaction: first, the brain tissue is subjected to antigen repair treatment, and then the silver staining technology is adopted, which significantly improves the signal-to-noise ratio of the senile plaque region, and ideal staining effect is obtained, which can effectively improve the detail visibility in pathological detection, help more accurately evaluate the morphology and distribution characteristics of the senile plaque, and has a wide application prospect.

The utility model discloses antigen repair equipment. The antigen repair equipment comprises a heating cavity and an incubation cavity, two symmetrically arranged and movable semiconductor chilling plate modules are arranged in the heating cavity, and the semiconductor chilling plate modules can move relatively; when the antigen repair equipment is in a heating working condition, the two semiconductor chilling plate modules move in opposite directions, so that the second sides are tightly attached to the two sides of a container filled with repair liquid outside the tissue slice, and the container is directly heated; the air collecting shell is used for collecting cold air of the semiconductor chilling plate module and conveying the cold air to the incubation cavity through the first hose, and the incubation cavity is used for pre-cooling or refrigerating tissue slices in the incubation cavity. According to the utility model, the antigen repair efficiency can be greatly improved, the film making probability can be reduced, and the antigen repair quality is improved.

The invention relates to an immune cytochemical antigen repair liquid for liquid-based cells and a use method of the immune cytochemical antigen repair liquid, and belongs to the technical field of immunohistochemistry. The antigen repair liquid disclosed by the invention is prepared from tris (hydroxymethyl) aminomethane, ethylenediamine tetraacetic acid disodium salt, urea, boric acid, 2-methyl-4-isothiazolin-3-ketone, a nonionic surfactant, water and alkali metal hydroxide. The liquid-based cell antigen repairing liquid is good in repairing effect, repaired cell sheets are easy to dye, and the liquid-based cell antigen repairing liquid has the advantages of high immunohistochemical dyeing quality, accurate positive positioning, low non-specific background, clear background and good repeatability, safety and stability, so that the effects of improving the antigen detection rate, sterilizing and removing enzyme are achieved; therefore, the immunocytochemical staining of the liquid-based cytology sample can be adapted.

The techniques provided herein relate to a method for antigen retrieval of fixed samples, particularly freeze-fixed tissue samples, by using a two-step demasking approach, in which the biological sample is treated at low temperatures (50-75°C) with different buffers.

The application discloses a kind of dewaxing, hydration and antigen repair integrated buffer for immunohistochemical detection, kit and application thereof, belong to immunodetection technical field.The dewaxing, hydration and antigen repair integrated buffer includes Tris Base, EDTA, nonylphenol polyoxyethylene ether, preservative, proteinase K and polyethylene glycol 2000.The dewaxing, hydration and antigen repair integrated buffer of the application is used to carry out paraffin section processing, can significantly shorten the time of immunohistochemical detection, so that antigen staining is more efficient.Immunohistochemical detection is carried out using the application, without using xylene and alcohol and other organic solvents in the process, more environmentally friendly, and, staining intensity is more obvious, more easily observed, has clinical auxiliary diagnosis.

The invention relates to the technical field of biomedical detection, and particularly discloses a multiple histochemical staining method based on controllable staining-decolorizing circulation. The method is realized through the steps of water bath-assisted antigen repair, composite pretreatment, alternate labeling dyeing of nanogold and quantum dots, electrochemical-assisted controllable decoloration, dynamic monitoring circulation, optical transparency treatment, multi-modal imaging reconstruction and the like. The key composition comprises a composite decolorizing agent containing a specific electron shuttling medium and a series of coloring agents containing nanogold and quantum dots. The preparation method mainly comprises the following steps: performing incubation reaction on each component and a tissue sample according to a specific sequence, completing controllable decolorization circulation under the assistance of an electric field, and regulating and controlling the process in real time through an optical monitoring system. According to the method disclosed by the invention, the sequential marking and accurate recognition of multiple target molecules on the same tissue sample can be realized; in addition, the preparation method has the advantages of being high in process controllability, good in dyeing cycle stability and high in operation repeatability.

The application belongs to the field of biological detection, and particularly relates to a TUNEL-TSA multiplex fluorescence staining method based on TSA technology optimization and application thereof. Tyramide signal amplification technology (TSA) is used to improve and optimize TUNEL combined immunofluorescence staining. The TSA technology can make the fluorescence signal intensity increase by more than 10 times, while maintaining a more complete cell morphological structure. In the multiplex staining process, the sequential scheme of "antigen retrieval-TSA fluorescence staining-protease K digestion-TUNEL staining" is significantly better than other staining strategies. It provides reliable technical support for establishing a standardized detection system, and has important clinical application and popularization value.