413 results about "Virus antigen" patented technology

The invention provides a porcine pseudorabies virus gene deletion strain, a vaccine composition, and a preparation method and an application of the vaccine composition. The vaccine composition comprises an immunizing dose of an attenuated livetotivirus antigen and an inactivated totivirus antigen of the porcine pseudorabies virus gene deletion strain or its culture. The vaccine composition can effectively induce the antibody production, can effectively protect pigs, and can be used as a marking vaccine to effectively differentiate wild strains and vaccine strains.

A freeze-dried high-temp. resistant protecting agent for virus-type biologic products for veterinary medicine is prepared from several components through proportionally mixing. It features that each of its components is sterilized separately. For the components which can be sterilized under high temp., they are dissolved in distilled water according to the proportion of formulation and are sterilized at 116 deg.C for 30-40 min; and for those which do not resist the high temp., they are also dissolved in distilled water according the proportion of formulation and are filtered with 0.22 micron pore diameter filter membrane to remove bacteria; and then the two parts are mixed to obtain the freeze-dried high-temp. resistant, protecting agent. The mentioned two parts are added to the virus antigen liquid according to a proportion of 1:1 and after packaging, they are freeze-dried. The product can be stored at 2-8 deg.C for 24 months.

The invention discloses a kit for detecting pig pseudorabies virus antibodies and a block enzyme-linked immuno sorbent assay (ELISA) method. The kit for detecting pig pseudorabies virus antibodies comprises pig pseudorabies virus monoclonal antibodies which are labelled by horseradish peroxidase, wherein the pig pseudorabies virus monoclonal antibodies are monoclonal antibodies obtained by pig pseudorabies viruses as immunogens and the pig pseudorabies viruses are pseudorabies virus strain Ea. The kit for detecting pig pseudorabies virus antibodies also comprises an enzyme label plate, a sample diluent, negative and positive contrasts, a coloured solution, a washing solution, and a stopping solution. The block ELISA method comprises the following steps of 1, taking out a detection plate pre-coated with virus antigens from the kit for detecting pig pseudorabies virus antibodies, adding diluted blood serum needing to be detected into the detection plate pre-coated with the virus antigens, and simultaneously, setting negative and positive contrast apertures, 2, shaking up the diluted blood serum in the negative and the positive contrast apertures, shaking off a solution in the negative and the positive contrast apertures, and washing the detection plate by the washing solution, and 3, adding the pig pseudorabies virus monoclonal antibodies labelled by horseradish peroxidase into the negative and the positive contrast apertures, washing, adding the colored solution into the negative and the positive contrast apertures to carry out room-temperature coloration in the dark, adding the stopping solution into the negative and the positive contrast apertures, and determining OD630nm values of the negative and the positive contrast apertures by an ELISA apparatus. The block ELISA method has the advantages of good singularity, high sensitivity, short detection time, and high accuracy because of utilization of an S/N ratio method in result determination.

This invention provides a trivalent immunogenic composition including a soluble portion of a Mycoplasma hyopneumoniae (M. hyo) whole cell preparation; a porcine circovirus type 2 (PCV2) antigen; and a PRRS virus antigen, wherein the soluble portion of the M. hyo preparation is substantially free of both (i) IgG and (ii) immunocomplexes comprised of antigen bound to immunoglobulin.

The present invention relates to the treatment of viral infections, particularly HBV and HCV infections, with a combination comprising a vaccine against a virus antigen and compounds that inhibit glucosidase activity in the host cell.

The invention discloses a bactrian camel heavy-chain (HC) variable-domain antibody resisting a porcine circovirus 2 as well as a preparation method and an application thereof, and belongs to the field of a biotechnology. A PCV2 (Porcine Circovirus Virus) inactivated vaccine immunized bactrian camel is firstly utilized and a phage display technology is utilized to establish a PCV2 immune VHH antibody base; and a high-specificity variable domain antibody of heavy chains of HCAbs (VHH) which has a good affinity with the PCV, particularly a PCV2 virus antigen; and the antibody disclosed by the invention has an amino acid sequence shown as SEQ (sequence) ID NO.1 or an amino acid sequence which is shown as SEQ ID NO.2 and has the sequence isogeny being more than 95%. The PCV2 heavy-chain variable-domain antibody disclosed by the invention has the very important meanings on researching porcine circovirus, particularly researching a porcine circovirus 2-type infection mechanism and establishing rapid antigen detection with high sensitivity and specificity or diagnosing a reagent.

The invention discloses combined detection test paper of influenza A virus antigen and influenza B virus antigen and a preparation method thereof. The test paper comprises a sample pad, a fiberglass membrane containing a colloidal gold particle label, a nitrocellulose membrane and water absorbing paper, wherein the nitrocellulose membrane comprises a detection area which is coated with an influenza A virus antibody, a detection area which is coated with an influenza B virus antibody and a control area which is coated with an goat anti-rabbit antibody; the colloidal gold particle label comprises a micro signal amplification system and a colloidal gold labeled rabbit IgG antibody; and the micro signal amplification system is a colloidal gold particle-avidin-biotin-influenza A/B virus antibody. According to the invention, an avidin-biotin microsignal amplification system is added in a double-antibody sandwich detection system, the signal of a target antibody is enlarged, the detection sensitivity is increased, false negative or detection omission due to weak signals can be avoided, simultaneously combined detection can be carried out on the influenza A and B virus antigens, and the detection time, sample and cost can be saved.

The invention discloses a method for preparing an anti-duck viral hepatitis transfer factor, which comprises the following steps of: firstly, inoculating duck viral hepatitis in a chick embryo allantoic cavity for multiplication and extracting a virus antigen from the chick embryo allantoic cavity; secondly, immunizing a healthy pig body with the extracted virus antigen; and thirdly, extracting the anti-duck viral hepatitis transfer factor from the spleen of the immune pig. In the invention, specific transfer factor is extracted from duck viral hepatitis viruses and can effectively suppress the duck viral hepatitis viruses and protect the cells of a normal body from being infected with the duck viral hepatitis viruses, so that the duck viral hepatitis is prevented and treated radically. Moreover, the preparation method of the invention is simple and feasible, short in production time and low in cost and has a promising application prospect.

The invention relates to a double-antibody biotin-Avidin ELISA (enzyme-linked immuno sorbent assay) detection kit for cattle viral diarrhea virus antigen and an application method thereof. The kit comprises washing liquid for ELISA detection, developing liquid, stopping liquid, Streptavidin-HRP, positive control and negative control and is characterized by further comprising an ELISA plate coating cattle viral diarrhea virus single antibody 3D8 and a cattle viral diarrhea virus single antibody 3F9 marked by biotin. The detection without cross reaction is performed by using the kit, thereby being capable of preventing leak detection caused by low titer in the antibody and having high specificity; a biotin-affine sensitizer is used for amplification, thereby increasing the flexibility, improving the detection rate, and simultaneously reducing the amount of second antibody; and the kit is suitable for being used widely.

The invention relates to an EV71 virus neutralization epitope detection kit or a reagent and a preparation method thereof. Particularly, the invention performs quantitative detection on EV71 virus antigen by adopting an HD6 monoclonal antibody with spectral activity. The kit or the reagent has the advantages of easy operation, high sensitivity and the like.

The embodiment of the invention provides a biosensor, a preparation method, a virus detection system and method. The method comprises the following steps: modifying a virus antigen or a virus antibodyor a nucleic acid probe for detecting viruses on a biosensor through bridging molecules capable of connecting various biomacromolecules, inputting a to-be-detected sample into the biosensor, and analyzing current signals before and after reaction, so as to detect whether viruses exist in the to-be-detected sample or not. The bridging molecule of the biosensor provided by the embodiment of the invention can be connected with various biomacromolecules, so that different viruses can be detected.

The invention discloses two monoclonal antibodies west nile virus and an relative application, wherein the monoclonal antibody is secreted from mice hybridoma cell WNV-2F5 of preservation number CGMCC No.2261 and mice hybridoma cell WNV-4B3 of preservation number CGMCC No.2262. The antigenic determinant of the monoclonal antibody is the third structural field of membrane protein E on west nile virus envelope. The monoclonal antibody is selected by indirect enzyme linked immunity method, analyzed by western blot, detected by flow cytometry, analyzed by surface plasma resonance and indirect immunity fluorescence method to completely analyze the specificity and affinity of combination with antigen, the antibody is marked by bioepiderm to build an antigen trap indirect enzyme linked immunity detection system. The inventive monoclonal antibody can be applied in various west nile virus antigen and in the test agent box preparation of west nile virus.

The invention discloses a method for producing PRRS (Porcine Reproductive and Respiratory Syndrome) viruses through culturing Marc-145 cells by applying a torrent-perfusion bioreactor. The method comprises the following steps of: (1) selecting Marc-145 cells as cells for culturing seedlings; (2) subculturing the Marc-145 cells; (3) reproducing virus seeds for production; (4) perfusing and culturing the Marc-145 cells on a paper carrier in the torrent-perfusion bioreactor; (5) producing the PRRS viruses in the torrent-perfusion bioreactor; and (6) treating the obtained virus antigen solution. The invention can greatly decrease the production cost, improve the input-output ratio by more than 10 times and expand the production scale easily and rapidly, has short production preparation cycle and high degree of automation, occupies less area, causes less environmental pollution which is easy to treat, consumes less labor, is easy to realize balanced and stable virus quality and can obviously improve the yield and the quality of the viruses.

A method for treating a virus-containing sample, characterized by treatment of a virus-containing sample with a treatment solution containing (1) an anionic surfactant and (2) an amphoteric surfactant, nonionic surfactant or protein denaturant; a virus assay method using said treating method; a method for treating a virus-containing sample, characterized by treatment of a virus-containing sample with a treatment solution containing (1) a chaotropic ion and (2) an acidifying agent; a virus assay method using said treating method; a virus assay method, characterized in that a virus antigen and a virus antibody are measured based on their binding to their probe in the presence of a surfactant with an alkyl group of 10 or more carbon atoms and a secondary, tertiary or quaternary amine, or a nonionic surfactant, or of both of them; and a monoclonal antibody and a hybridoma producing the same for carrying out said method.

The invention relates to a lipid microsphere composition. The lipid microsphere composition is characterized by comprising a lipid microsphere emulsifying agent and an immunogenic composition, wherein the lipid microsphere emulsifying agent comprises 1 to 30 weight percent of pharmaceutical grade grease, 0.1 to 10 weight percent of zwitterionic/non-ionic surfactant composition, 0.01 to 5 weight percent of stabilizing agent and the balance of aqueous solution; and the immunogenic composition is a univalent to multivalent composition and usually a trivalent composition, and contains a virus antigen or antigenic preparation from one or more pandemic influenza epidemic related influenza virus strains or with one or more pandemic influenza epidemic related influenza virus strains. The lipid microsphere composition not only can serve as an excellent aid of a vaccine, but also can serve as a conveying system of the vaccine. The lipid microsphere composition is applicable to different administration routes of the vaccine, improves compliance, diversity and selectivity of clinical application, and achieves wide, safe and effective immune effect.

The invention provides O-type aftosa synthetic peptide vaccine, and in particular relates to polypeptide or polypeptide polymer thereof used in the vaccine as well as the vaccine containing the polypeptide or the polypeptide polymer thereof and a preparation method thereof. The polypeptide has amino acid sequences shown in SEQ ID No.1, SEQ ID No.2 and SEQ ID No.3. The O-type aftosa synthetic peptide vaccine carries out chemical synthesis of potential antigen site peptide segments by carrying out sequencing of domestic aftosa epidemic strains to study the variation of the main antigen sites ofaftosa and combining with computer assistant to carry out antigen site analytical prediction. Candidate polypeptide antigens are screened out by carrying out large numbers of animal experiments and aftosa virus antigen sites are optimized according to the screening result; and T cell epitope and B cell epitope are effectively combined to improve the immune effects of the polypeptide antigens. TheO-type aftosa synthetic peptide vaccine can effectively cope with the antigen variation of aftosa virus and has ideal biosafety and easy large-scale synthesis, thereby having a good application prospect.

The invention provides a foot and mouth disease virus antigen polypeptide, a foot and mouth disease virus fusion antigen polypeptide and a foot and mouth disease virus vaccine containing the antigen polypeptide and/or the fusion antigen polypeptide. The invention further provides application of the antigen polypeptide, the fusion antigen polypeptide and the vaccine in prevention and control of foot and mouth disease virus infection. The foot and mouth disease virus antigen polypeptide, the fusion antigen polypeptide and the vaccine have broad-spectrum immunogenicity, and can generate good immunogenicity on different foot and mouth disease viruses and variant strains thereof.

The invention relates to a freeze-dried rabies vaccine for humans and a preparation method of the vaccine, relates to the field of vaccine production preparation technologies and aims at solving the problems that effective virus antigen expression content is low, the side effect rate of a vaccinator is high and vaccine yield and quality can not meet standard requirements as only a biological reactor is adopted for producing a rabies vaccine. The freeze-dried rabies vaccine for humans is obtained by inoculating aG strain rabies virus on Vero cells and sequentially carrying out ultrafiltration and concentration, separation and purification as well as freeze drying, wherein the packing volume of the freezed-dried rabies vaccine for human use is 0.5ml/dose, and during freeze drying, the adopted vaccine freeze-drying protecting agent comprises the following ingredients: 60-90g/l of trehalos, 6-14g/l of sodium glutamate, 3-6g/l of urea, 2-3g/l of L-arginine and 10g/l of 199 culture medium, and the vaccine freeze-drying protecting agent does not contain gelatin, human serum albumin or dextran. The freeze-dried rabies vaccine for humans has the advantages that cost is low, operation is easy, pollution is hardly produced, vaccine quality and yield are greatly improved, the content of impurities in a vaccine is reduced, allergy reactions are hardly caused, and vaccine safety is greatly improved.

The invention supplies a rapid testing tape that could simultaneously test A, B virus antigen. It covers FluA-McAb, FluB-McAb and IgG on NC film, compounds FluA-McAb, FluB-McAb marked by colloidal gold, simultaneously tests A, B virus antigen in tested sample by using film chromatography double antibody cream filling method. The invention could decrease cockamamie process, and could gain result in 10-15 minutes. It is suitable to hospital and the research for large scale epidemiology to handle sporadic affair.

The invention provides a multivalent immunogenic composition containing enterovirus antigens. The composition comprises inactivated EV71 antigens and/or inactivated CA16 antigens, and inactivated polio antigens. The composition can further comprise antigens selected from hepatitis A antigens, hepatitis B antigens, acellular pertussis antigens, tetanus toxoid, diphtheria toxoid, Haemophilus influenzae type b capsular polysaccharide, and meningococcal polysaccharide antigens, as well as physiologically acceptable carriers combined with bacterial polysaccharide antigens. The invention also provides a preparation method of the composition. The composition can prevent invasion of a plurality of pathogens simultaneously without interference among the antigens, and the immunogenicity is no less than that of individually activated antigens. With the composition, vaccination processes are significantly simplified, and the vaccination efficiency is improved with reduced costs.