Bactrian camel heavy-chain (HC) variable-domain antibody resisting porcine circovirus 2 as well as preparation method and application thereof
A technology of porcine circovirus and single-domain heavy chain antibody, which is applied in the fields of virus/phage, antiviral immunoglobulin, botany equipment and methods, etc.
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[0056] Example 1 Preparation of Bactrian camel VHH heavy chain antibody against porcine circovirus type 2
[0057] 1. Materials and methods
[0058] 1.1 Materials
[0059] 1.1.1 Test animals, antigens, adjuvants
[0060] 1 male Bactrian camel (1 year old), 1 female Bactrian camel (6 months old); PCV2 inactivated vaccine (Ingelvac circoFLEX, Serial NO.309-211), recombinant PCV2 VLPs antigen (Shuanghui Yin, Shiqi Sun, Shunli Yang, et al. Self-assembly of virus-like particles of porcine circovirus type 2capsid protein expressed from Escherichia coli [J.] Virology Journal 2010,7:166).
[0061] 1.1.2 Strains, helper phages and reagents
[0062] Escherichia coli TG1 and HB2151, helper phage M13K07, and carrier pHEN2 were purchased from Novagen; expression vector pSMK was donated by Dr. Sun Shiqi from our institute; restriction enzymes were purchased from NEB; lymphocyte separation solution (1.077) was purchased from Shanghai Sangong; Trizol, DNA fragment recovery kit and plasmid...
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