190results about How to "Lethal" patented technology

The invention provides a humanized PD-L1 tumor cell line MC-38-hPD-L1, a builtanimal tumor model with the same and a method for constructing the humanized PD-L1 tumor cell line. The method particularly includes knocking out animal-origin PD-L1 by the aid of CRISPR-CAS9; carrying out amplification and cultivation to obtain knocked-out cell banks; extracting DNA (deoxyribonucleic acid) and carrying out PCR (polymerase chain reaction) amplification; recycling and cloning amplification products; carrying out over-expression on human-origin PD-L1 in MC-38 cell lines of mPD-L1 KO by the aid of lentivirus systems; packaging lentivirus and screening Puromycin to obtain the humanized MC-38 cell line of PD-L1. The humanized PD-L1 tumor cell line, the animal tumor model and the method have the advantages that as shown by results, high killing efficiency and multiplication capacity are obviously presented by tumor infiltration CD8 T lymphocytes after antibody treatment is carried out, tumor infiltration Treg cells can be obviously inhibited after antibody treatment is carried out, and accordingly the method is proved to be effective and feasible from the aspect of molecular mechanisms.

The invention belongs to the field of aseptic technologies integrating compound natural aseptic and bacteriostatic agents and flexible sterilizing conditions, and relates to a preparation method for an aseptic containing compound clove / cinnamon extracts and the aseptic application of the aseptic in sauce products. The compound clove / cinnamon extracts are firstly prepared, and the aseptic containing the compound clove / cinnamon extracts is prepared afterwards; the aseptic application of the aseptic in sauce products is characterized in that the addition of the aseptic with a mass fraction of 0.2 percent is integrated with the condition that sterilization is carried out for 20-30 minutes at a temperature of 85 DEG C for the aseptic and bacteriostatic purposes, so as to achieve the one-year shelf life of sauce products and good bacteriostatic and aseptic effects. According to the invention, the compound clove / cinnamon extracts have the advantages of high safety and wide bacteriostatic range, and the aseptic containing the compound clove / cinnamon extracts has high water solubility and stability; the aseptic method can not only kill microorganisms in sauce products but also better preserve nutrients in sauce products, and is an aseptic approach remarkably integrating natural aseptic materials and flexible sterilization, thereby achieving broad application prospects.

The serial pig raising compound Chinese herbal medicine mixtures have different compounding proportions for the requirement of pig in different growth stages, including sucking pig, piglet, adult pig and hog. The mixtures have the main ingredients of vitamins, choline, trace elements, lysine, calcium biphosphate, stone powder, table salt and 12 kinds of Chinese medicinal materials, including haw, malt, andrographis herb, etc. The mixtures can prevent and treat diseases of pig, raise pigí»s immunity and promote pigí»s growth, has less side effect and less resistance, and is favorable to production of green pork food.

The invention relates to exosome as well as a preparation method and application thereof in preparing medicines for treating lung cancer. The preparation method comprises a step of stimulating cells to secrete the exosome by using KRN7000 and ATP (Adenosine Triphosphate). By adopting the preparation method provided by the invention, efficient in-vitro exosome preparation can be achieved. The invention further provides the exosome prepared by using the method. The exosome has relatively good cytotoxic effects and lung tumor resistance activity. The invention further provides application of the exosome in preparing medicines for treating lung cancer.

The invention relates to a method for preparing photocatalyst nanometer wood-plastic boards. The method comprises the following steps: a) 50 to 100 portions by weight of wood meal and 2 to 4 portions by weight of aluminum-zirconium treatment agent are added in turn to a horizontal stirrer and stirred for 2 to 3 hours at a temperature between 100 and 120 DEG C; b) the treated wood meal and resin are put into a high-speed kneader in the mass ratio of 1:2, mixed with 7 to 40 portions by weight of additive, and are stirred for 10 to 15 minutes at the temperature between 100 and 120 DEG C, unloaded to a low-speed mixer and stirred to normal temperature; c) the mixed materials are put into a board extrusion device and subjected to vacuum and cooling setting and two-roller calendering setting so as to product a board, and the board is cut with fixed-length; and d) the surface of the board is sprayed with a layer of photocatalyst nanometer material, so as to obtain a finished product. The method has the advantages of low cost, extensive resource and good decorative effect, and more importantly, the method can utilize natural light to sterilize, thereby achieving the effect of purifying air.

The invention discloses lymantria dispar linnaeus CYP6B53 gene dsRNA and application thereof in nuisanceless control. The dsRNA sequence is shown by SEQ ID NO.3, and can be applied to inhibition of the growth and development of lymantria dispar linnaeus or improvement of the sensitivity of the lymantria dispar linnaeus to an organic phosphorus insecticide. An experimental result shows that after the CYP6B53 gene dsRNA is injected into a lymantria dispar linnaeus larva, compared with a contrast (injected with ddH2O and GFP gene dsRNA), a relatively strong silencing effect is achieved, so that the growth and development of the lymantria dispar linnaeus larva are inhibited and then the lymantria dispar linnaeus larva dies. Therefore, the invention provides a silencing technology capable of using CYP6B53 to control an important forest pest, namely the lymantria dispar linnaeus and lepidopterous pests with similar structural domains, thereby providing a new train of thought and a new technology for green control of forest pests.

The invention discloses a special seed dressing agent with effects of increasing the survival rate and invigorating seedlings for sweet corn. The seed dressing agent comprises gamma-aminobutyric acid, matrine, thiamethoxam, sedaxane, carboxin, amino-oligosaccharin, salicylic acid, glycine betaine, choline chloride, fulvic acid, starch grafted potassium acrylate, calcium chloride, a wetting dispersant, an emulsifier, gibberellin and microelement water soluble fertilizer mother liquor. Under normal conditions or adverse conditions of drought, low temperature and the like, the seed dressing agent has an obvious effect of improving the seed vigor and seedling quality of the sweet corn, can be used for effectively increasing the yield of the fresh spike of the sweet corn, also has effects of exterminating underground insects, protecting and invigorating seedlings and preventing and treating main plant diseases and insect pests of the corn, has control efficiency up to 70%-90% for plant diseases and insect pests in a seedling stage, has control efficiency up to 87%-96% for corn head smut, and not only can help farmers to save labor cost and seed cost greatly, but also can increase the yield by 15%-20%, thus having excellent social and economic benefits and application prospects.

The invention relates to a traditional Chinese medicine insecticide with an ecological balance improving function, which belongs to the technical field of agriculture. The traditional Chinese medicine pesticide is used for solving the defect that the chemical insecticide of the prior art is harmful to both the human body and the environment, and mainly comprises the following components by weight: 2 kilograms of coptis chinensis, 2 kilograms of gentiana scabra bunge, 1 kilogram of sophora flavescens, 2 kilograms of kadsura root-bank, 2 kilograms of golden larch bark, 1 kilogram of stellera chamaejasme, 1 kilogram of fructus cnidii, 2 kilograms of sulphur, 0.6 kilogram of quicklime, 3 kilograms of radix aconiti agrestis and 1 kilogram of tobacco, then, 50 kilograms of water is added to stew at slow fire until 30 kilograms of the insecticide liquid is left, and preparation can be carried out according to the insecticide liquid-to-water ratio of 1:50. The traditional Chinese medicine insecticide has a durable pesticide effect, and has no pollution, no harm and no residual to both the human body and the environment, so the soil is enabled to be loosened, the crop growth is benefited, and the traditional Chinese medicine insecticide has a certain sterilization function to water, no pollution to the air and an effect of improving the ecological environment.

The present invention relates to one kind of pesticide composite, and the synergistic botanical orange terpene pesticide consists of orange terpene in 3-30 wt%, pyrethroid in 0-4 wt% and biological pesticide in 0-10 wt% as the effective components, as well as assistant, emulsifier and deionized water. All the components are emulsified in stirring emulsifier to form the emulsified product, which is mixed with water and sprayed to prevent various indoor and outdoor pests. The pesticide composition has low toxicity and may be used in public fields and fruits and vegetable.

The invention provides a medical sterilization ultrasonic coupling agent and a preparation method thereof. The coupling agent comprises the following main components by the weight percentage: 0.2-0.6% of p-chlor-m-xylenol, 4-8% of propylene glycol, 4-8% of glycerine, 0.3-1.2% of crosslinking polyacrylic acid resin, 0.2-1.2% of sodium hydroxide, and 80-90% of distilled water; the preparation method thereof comprises the steps: (1) dissolving crosslinking polyacrylic acid resin in distilled water to obtain liquor A; (2) dissolving p-chlor-m-xylenol in propylene glycol to obtain liquor B; (3) mixing and stirring the liquor A and liquor B, neutralizing with sodium hydroxide and adding glycerine to obtain the finished product. The invention not only meets the property and functions of medical sterilization ultrasonic coupling agent, but also has efficient and disinfectant effect and causes an ultrasonic probe to always maintain at the disinfectant state, thus completely solving the problem that the ultrasonic probe can not realize the effective disinfection in short time. The invention reduces the risk of the mutual pathogenic bacteria cross-infection among ultrasonic examinees and is beneficial to the health of people.

The invention relates to polypeptide capable of specifically targeting HER2 (human epidermal growth factor receptor 2) protein and an application of the polypeptide. The polypeptide is shown by the following general formula: X1X2X3X4X5LX6X7X8NPX9X10X11X12X13. The invention also relates to a nucleotide sequence for coding the polypeptide, and an expression vector for expressing the polypeptide, and host cells; and the invention also relates to the polypeptide, a bivalent or a multivalent formed by the polypeptide, and a pharmaceutical composition which is formed by the polypeptide serving as a targeting polypeptide, a preparation capable of killing cancer cells, and an imaging preparation, as well as applications of the polypeptide, the bivalent or the multivalent, and the pharmaceutical composition. The polypeptide provided by the invention can achieve a targeting effect on positive tumor cells of HER2, is strong in selectivity, can be prepared by adopting a chemical synthesis method, is high in purity, small in molecular weight and strong in specificity, and is free of immunogenicity, safe and reliable.

The invention is concerned wih the food combination and preparation method for recovering from fatigue and invigorating the stomach, the compositions and ratio is: codonopsis pilosula, Chinese yam rhizome, hoantchy root, balsam pear and almond, which is better for refresh and recover from fatigue, lower price, easy to eat.

The invention discloses a high-purity allogeneic NK cell culture medium and an in-vitro amplification method. Mononuclear cells are separated from placenta blood through a lymphocyte separation liquid, and the separated mononuclear cells are added into a serum-free immune cell culture medium system containing a CD314 antibody, IL-2, inactivated autologous plasma and the like to be cultured for 3 days; and 10% inactivated autologous plasma and 1000 IU / mL IL-2 are added once on the fourth day and the fifth day of culture respectively for culture, massive amplification of cells can be seen after continuous culture is performed for 7 days, continuous culture is performed for 2 days, and addition factors are supplemented as required. A culture bottle does not need to be coated in advance, feeder layer cells do not need to be used, the expression rate of the prepared NK cells CD3-CD16+ / CD56+ reaches up to 90% or above after 14-16 days of culture, the in-vitro tumor killing activity is high, and the problems that in an existing NK culture system and technology, the purity of the NK cells is low, and the killing activity is poor can be solved.

The invention provides a kieselguhr insecticide, and a preparation method and application thereof. The kieselguhr insecticide comprises the following components in percentage by mass: 80-91 percent of kieselguhr powder, 1.5-3.5 percent of pyrethrum powder and 7.5-16.5 percent of sepiolite powder. The insecticide contains simple components, is high in insecticide efficiency, safe, low in toxicity and low in residue, and is an environment-friendly natural plant insecticide. The insecticide is extremely powerful in lethality to software insects, and can be widely applied to storage of grains and seeds and prevention and treatment of plant diseases and insect pests of crop fruit, vegetable and food production; meanwhile, the insecticide preparation method is simple and is suitable for industrial production.

The invention discloses a making method of organic metal carborane targeting agent based on magnetic nanometer material, which is characterized by the following: proceeding self-assemblage for magnetic nanometer particle Fe3O4 and one / two meal central carborane with surface decorated; making targeting agent move to tumour position under external magnetic field.

Disclosed is a method for preparing activated lymphocytes, which comprises isolating lymphocytes from peripheral blood and proliferating and activating the isolated lymphocytes in vitro. According to the disclosed method, highly effective toxic cells can be prepared in large amounts by culturing human peripheral lymphocytes in the presence of an anti-CD3 antibody, IFN-gamma and IL-2. The activated lymphocytes proliferated according to the disclosed preparation method comprise both CD3-CD56+ (natural killer cell marker) cells that are the main components of LAK cells, and CD3+CD56+ cells that are the main components of CIK cells, and can be cultured in large amounts. Thus, the lymphocyte cells can show a significantly higher anticancer effect compared to when the LAK cells and the CIK cells are used alone.

The invention discloses a preparation method and application of water-soluble titanium dioxide nanoparticles. According to the preparation method, beta-cyclodextrin is used as an initial reaction material, a macroinitiator is synthesized by means of modification, a multi-arm star-shaped block copolymer is prepared by using an atom transfer radical polymerization (ATRP) technology and a technologywhich combines ATRP with 'click' chemistry, and the water-soluble titanium dioxide nanoparticles with uniform sizes and good dispersibility are prepared by taking the multi-arm star-shaped block copolymer as a monomolecular micelle template on the basis of a solution phase synthesis method. The titanium dioxide nanoparticles prepared by the preparation method are stable in quality, uniform in particle sizes, and can be stabilized at a nanoscale; the properties of the titanium dioxide nanoparticles can be effectively enabled to be exerted. The surfaces of the titanium dioxide nanoparticles aremodified, so that the titanium dioxide nanoparticles can be enabled to be evenly dispersed in water, and an aggregation effect is further avoided.

An automatically changing saw blade unit used as the obstacle in robot fight game to destroy the robot is composed of pneumatic control system, cylinder, piston and its connecting rod, motor, saw blade, rotary supporter and electric control system with electromagnetic valve fixed to said cylinder.

The present invention relates to a biological insecticide. Currently, the broad use of poisonous pesticide causes the damage to the ecological agriculture. The toxicity caused by residual pesticide, especially the toxicity in the food enters the human body continuously. Therefore the continual using of the chemical pesticide goes against the environment and the existence of human being. The invention is prepared by the biological raw materials and has no poison or residual. The biological insecticide goes into the stomach and intestine of the destructive insect mainly through the air and has a stomach insecticidal effect. The insecticidal effect is strong and the efficiency is higher than 90%. The biological insecticide according to the invention also has the function of flower and fruit protection and invigorating the seed, and the purposes of production increase and income increase can be obtained. The product also has advantages of high toxicity, without any pesticide resistance generation and without any toxic and side effects to the fruit and vegetable. The biological insecticide according to the invention is an insecticide with advantages of health, environment protection and without pollution.

The invention relates to a preparation method of a nanometer preservative containing composite extracts and the corrosion resistance application of the nanometer preservative. According to the method disclosed by the invention, on the basis that composite clove / cinnamon / fresh ginger extracts (1:1-2:1-2) are extracted and compounded, the nanometer preservative containing the composite extracts is compounded, and the particle diameter can reach 8.69nm. The combination of the added 0.5g / kg of the nanometer preservative with the condition of disinfection for 15-20 minutes at the temperature of 85 DEG C can be applied to corrosion resistance and bacteriostasis of sauce products, so that the products can have the storage shelf life of one year. The nanometer preservative containing the composite clove / cinnamon / fresh ginger extracts, disclosed by the invention, has extensive bacteriostasis scopes, and has high safety, waster solubility and stability. The combination of the composite natural agent for corrosion resistance and bacteriostasis with flexible disinfection conditions can well maintain the nutrient substances and the flavor characteristics of products, and wide application prospects.

The invention discloses a multi-targeting antitumor composite preparation and a preparation method thereof. The multi-targeting antitumor composite preparation comprises a Fu vesicle suspension, DOX-BSA and FA-DOX. A vesicle administration system is prepared through integrating immunotherapy, positive targeting chemotherapy medicine drug molecules and a passive targeting administration system realize in order to realize low toxicity and high efficiency treatment of tumors, and the vesicle administration system can obviously slowly release Fu to effectively containment tumor development and has small influences on tumor bearing bodies; a newly synthesized immunogen can obtain an antibody with high sensitivity, so foundation is laid for formation of an immune compound macromolecule required by immunotherapy; and the micro-molecular compound FA-DOX has a molecule bridging effect in immunotherapy to promote formation of the immune compound macromolecule. The composite preparation has obviously better curative effects than simple particle administration chemical treatment or simple immunotherapy, has obviously low toxicity, can improve the antitumor effect, and is an anticancer composite preparation with wide research and application prospect.

The invention discloses an intelligent remote-control solar insecticidal lamp which comprises a solar cell panel (1), a controller, an energy storage cell, a rain shade (2), a lamp post (9), a base (8), an energy-saving insecticidal lamp (4), a high voltage power network (3), an insect collector (5) and an insect collection box (7). The intelligent remote-control solar insecticidal lamp is characterized in that the intelligent remote-control solar insecticidal lamp further comprises a fault detection unit, a main control unit, a cell capacity detection unit, full collection warning units (6) for the insect collection box, a network transmission unit and a remote monitoring host computer, wherein the high voltage power network is arranged at the periphery of the energy-saving insecticidal lamp; the rain shade is arranged at the upper part of the energy-saving insecticidal lamp, and the lower part of the energy-saving insecticidal lamp is connected with the insect collector; the lower part of the insect collector is connected with the insect collection box; the controller, the fault detection unit, the main controller and the cell capacity detection unit are arranged below the insect collector; the full collection warning units (6) for the insect collection box are arranged at the inner part of the insect collection box; and the energy storage cell is arranged in the base. The intelligent remote-control solar insecticidal lamp has the advantages that the design is reasonable, and by additionally mounting remote monitoring control equipment, the working state of the insecticidal lamp can be remotely monitored.

The invention relates to a capsule for preventing and treating AIDS and a preparation technique thereof, pertaining to biomedical field, in particular to a capsule with high-esterified catechin as the main active ingredient thereof and a preparation technique of a micro-capsule of the high-esterified catechin as well. Microencapsulation enhances the stability of the high-esterified catechin and covers the unpleasant odor and taste. AIDS is one of the five main causations of death all over the world. The product of the invention is applied in the treatment of AIDS, performs a rather strong lethality on the AIDS, and can be substituted for the expensive and high-toxic imported drugs, which is suitable for national conditions of China, thereby having considerable economic benefits and promising market prospect.

The invention relates to a pesticide, in particular to a pesticide for killing cotton bollworms. The technical proposal is as follows: the pesticide is mixed by phoxim, karbofos, sumicidin and an emulsifying agent and is characterized in that the weight percentage of the components is 20 to 40 percent of the phoxim, 20 to 40 percent of the karbofos, 10 to 30 percent of the sumicidin and 30 to 50 percent of the emulsifying agent, and the components of the phoxim, the karbofos, the sumicidin and the emulsifying agent are put in a glass vessel to be mixed evenly according to the mixture ratio. The pesticide has the advantages of strong killing ability, high drug effect and low cost.

The invention belongs to the field of targeted drug delivery systems, and particularly relates to a targeting thermosensitive liposome combination. The targeting thermosensitive liposome combination is characterized in that: the combination comprises thermosensitive liposome, the surface of which is modified by tumor homing peptide CREKA (cys-arg-glu-lys-ala) or peptide with a CREKA core sequence, and the thermosensitive liposome contains antitumor medicine. The invention also relates to a preparation method of the targeting thermosensitive liposome combination and an application of the targeting thermosensitive liposome combination in the preparation of drugs for treating malignant tumors. The targeting thermosensitive liposome has a good tumor-targeting effect and a good antitumor effect.

The invention discloses a drug for treating and / or preventing tumor. The drug comprises active components, namely a component A and a component B, wherein the component A is ascorbic acid soluble salt or ascorbic acid, and the component B is disulfiram. The compound drug which comprises sodium ascorbate and the disulfiram can effectively inhibit the growth of in-vivo in-vitro cancer cells, achieves the synergistic effect, achieves the selective killing effect on tumor cells and can be used as a low-toxicity effective compound drug for treating and / or preventing the tumor.

The invention relates to a povidone iodine five-compound disinfectant which comprises the following components in percentage by weight: 5-20 percent of povidone lodine, 0.1-10 percent of medical glycerin, 0.1-2 percent of citric acid, 0.1-1 percent of nonoxynol-10, 0.01-0.1 percent of sodium hydroxide and the balance of water for injection. The povidone iodine five-compound disinfectant can be used for producing povidone iodine compound wipes with different specifications, povidone iodine compound cotton swabs, povidone iodine compound spongy cushions, povidone iodine compound hand-washing brushes and the like; and after the products are specially treated or sterilized, the sterility and the safety of the products are fully ensured, and after being unpacked, the products can be directly used, so that the cross contamination and the infection in an intermediate link are avoided. The povidone iodine five-compound disinfectant has the advantages of small skin irritation, low toxicity, lasting action, and safety and convenience in use; the povidone lodine five-compound disinfectant basically has no irritation to tissues and is used for skin and mucosa disinfection, such as cleaning before operation, operative site and wound disinfection, and skin cleaning and disinfection before and after arterial blood drawing.

The invention relates to a biochemical pesticide mixed fatty acid preparation and its application. The mixed fatty acid preparation contains 1-30% mixed fatty acid and 70-99% auxiliary agents based on the total weight of the mixed fatty acid preparation; the auxiliary agents are surfactants, emulsifiers, antifreeze agents, dispersants and the like. The mixed fatty acid preparation of the present invention has an EC50 of 0.0479 mg / L for the tobacco virus disease, has strong lethality against the tobacco virus disease, and the control effect can reach more than 70% 14 days after administration. The mixed fatty acid preparation of the invention has the characteristics of high efficiency, low toxicity, low price and no environmental pollution, and has extremely broad market application prospects.

The invention relates to an embedded composite wallboard of an earthquake proof construction, comprising a shell, and a corrugated ribbed slab and packing which are arranged in the shell, wherein the shell is made of a glass magnalium composite board; the corrugated ribbed slab is made of aluminum plastic glass fiber reinforced plastics; the packing is made of the mixture of perlite powder, kieselguhr and magnesite powder; and the corrugated ribbed slab in the packing and the shell outside the packing are solidified together by using mucilage glue. Since the composite wallboard takes the light high-strength glass magnalium composite board as the shell, the light high-strength aluminum plastic glass fiber reinforced plastics corrugated ribbed slab as a reinforcing rib and the light, heat-insulation, sound-insulation and insulated mixture of the perlite powder, the kieselguhr and the magnesite powder as the packing, and the shell, the corrugated ribbed slab and the packing are solidified together by using the mucilage glue, the embedded composite wallboard has the properties of firmness and light weight and the characteristics of heat preservation, fire prevention and noise insulation. Especially when the embedded composite wallboard is put in use in a mode of small area and a building is damaged once an earthquake happens, the antipersonnel force to people can be reduced a lot, and therefore, the embedded composite wallboard is an ideal building material for the traditional earthquake proof construction.

The invention relates to a plasma air purifier and an air cleaning device employing same. A plasma generator of the plasma air purifier is arranged in upwind of a mediate effective filter screen or in a blower fan cabinet, and a high-resistance filtration system is arranged on the leeward side of the blower fan. After multiple forced reaction, the plasmas in the plasma air purifier is fully used, the hydroxy free radical generated only exists in the air purifier and may not be released to the indoor space. The plasma air purifier caneffectively disinfects, removes PM0.1 or smaller harmful particles. No hydroxy free radicals are remained to cause damage to human bodies, and the safety is high.