190results about How to "Lethal" patented technology

The invention provides a humanized PD-L1 tumor cell line MC-38-hPD-L1, a builtanimal tumor model with the same and a method for constructing the humanized PD-L1 tumor cell line. The method particularly includes knocking out animal-origin PD-L1 by the aid of CRISPR-CAS9; carrying out amplification and cultivation to obtain knocked-out cell banks; extracting DNA (deoxyribonucleic acid) and carrying out PCR (polymerase chain reaction) amplification; recycling and cloning amplification products; carrying out over-expression on human-origin PD-L1 in MC-38 cell lines of mPD-L1 KO by the aid of lentivirus systems; packaging lentivirus and screening Puromycin to obtain the humanized MC-38 cell line of PD-L1. The humanized PD-L1 tumor cell line, the animal tumor model and the method have the advantages that as shown by results, high killing efficiency and multiplication capacity are obviously presented by tumor infiltration CD8 T lymphocytes after antibody treatment is carried out, tumor infiltration Treg cells can be obviously inhibited after antibody treatment is carried out, and accordingly the method is proved to be effective and feasible from the aspect of molecular mechanisms.

The invention belongs to the field of aseptic technologies integrating compound natural aseptic and bacteriostatic agents and flexible sterilizing conditions, and relates to a preparation method for an aseptic containing compound clove/cinnamon extracts and the aseptic application of the aseptic in sauce products. The compound clove/cinnamon extracts are firstly prepared, and the aseptic containing the compound clove/cinnamon extracts is prepared afterwards; the aseptic application of the aseptic in sauce products is characterized in that the addition of the aseptic with a mass fraction of 0.2 percent is integrated with the condition that sterilization is carried out for 20-30 minutes at a temperature of 85 DEG C for the aseptic and bacteriostatic purposes, so as to achieve the one-year shelf life of sauce products and good bacteriostatic and aseptic effects. According to the invention, the compound clove/cinnamon extracts have the advantages of high safety and wide bacteriostatic range, and the aseptic containing the compound clove/cinnamon extracts has high water solubility and stability; the aseptic method can not only kill microorganisms in sauce products but also better preserve nutrients in sauce products, and is an aseptic approach remarkably integrating natural aseptic materials and flexible sterilization, thereby achieving broad application prospects.

The serial pig raising compound Chinese herbal medicine mixtures have different compounding proportions for the requirement of pig in different growth stages, including sucking pig, piglet, adult pig and hog. The mixtures have the main ingredients of vitamins, choline, trace elements, lysine, calcium biphosphate, stone powder, table salt and 12 kinds of Chinese medicinal materials, including haw, malt, andrographis herb, etc. The mixtures can prevent and treat diseases of pig, raise pigí»s immunity and promote pigí»s growth, has less side effect and less resistance, and is favorable to production of green pork food.

The invention discloses a special seed dressing agent with effects of increasing the survival rate and invigorating seedlings for sweet corn. The seed dressing agent comprises gamma-aminobutyric acid, matrine, thiamethoxam, sedaxane, carboxin, amino-oligosaccharin, salicylic acid, glycine betaine, choline chloride, fulvic acid, starch grafted potassium acrylate, calcium chloride, a wetting dispersant, an emulsifier, gibberellin and microelement water soluble fertilizer mother liquor. Under normal conditions or adverse conditions of drought, low temperature and the like, the seed dressing agent has an obvious effect of improving the seed vigor and seedling quality of the sweet corn, can be used for effectively increasing the yield of the fresh spike of the sweet corn, also has effects of exterminating underground insects, protecting and invigorating seedlings and preventing and treating main plant diseases and insect pests of the corn, has control efficiency up to 70%-90% for plant diseases and insect pests in a seedling stage, has control efficiency up to 87%-96% for corn head smut, and not only can help farmers to save labor cost and seed cost greatly, but also can increase the yield by 15%-20%, thus having excellent social and economic benefits and application prospects.

The invention relates to a traditional Chinese medicine insecticide with an ecological balance improving function, which belongs to the technical field of agriculture. The traditional Chinese medicine pesticide is used for solving the defect that the chemical insecticide of the prior art is harmful to both the human body and the environment, and mainly comprises the following components by weight: 2 kilograms of coptis chinensis, 2 kilograms of gentiana scabra bunge, 1 kilogram of sophora flavescens, 2 kilograms of kadsura root-bank, 2 kilograms of golden larch bark, 1 kilogram of stellera chamaejasme, 1 kilogram of fructus cnidii, 2 kilograms of sulphur, 0.6 kilogram of quicklime, 3 kilograms of radix aconiti agrestis and 1 kilogram of tobacco, then, 50 kilograms of water is added to stew at slow fire until 30 kilograms of the insecticide liquid is left, and preparation can be carried out according to the insecticide liquid-to-water ratio of 1:50. The traditional Chinese medicine insecticide has a durable pesticide effect, and has no pollution, no harm and no residual to both the human body and the environment, so the soil is enabled to be loosened, the crop growth is benefited, and the traditional Chinese medicine insecticide has a certain sterilization function to water, no pollution to the air and an effect of improving the ecological environment.

The present invention relates to one kind of pesticide composite, and the synergistic botanical orange terpene pesticide consists of orange terpene in 3-30 wt%, pyrethroid in 0-4 wt% and biological pesticide in 0-10 wt% as the effective components, as well as assistant, emulsifier and deionized water. All the components are emulsified in stirring emulsifier to form the emulsified product, which is mixed with water and sprayed to prevent various indoor and outdoor pests. The pesticide composition has low toxicity and may be used in public fields and fruits and vegetable.

The invention is concerned wih the food combination and preparation method for recovering from fatigue and invigorating the stomach, the compositions and ratio is: codonopsis pilosula, Chinese yam rhizome, hoantchy root, balsam pear and almond, which is better for refresh and recover from fatigue, lower price, easy to eat.

The invention discloses a high-purity allogeneic NK cell culture medium and an in-vitro amplification method. Mononuclear cells are separated from placenta blood through a lymphocyte separation liquid, and the separated mononuclear cells are added into a serum-free immune cell culture medium system containing a CD314 antibody, IL-2, inactivated autologous plasma and the like to be cultured for 3 days; and 10% inactivated autologous plasma and 1000 IU/mL IL-2 are added once on the fourth day and the fifth day of culture respectively for culture, massive amplification of cells can be seen after continuous culture is performed for 7 days, continuous culture is performed for 2 days, and addition factors are supplemented as required. A culture bottle does not need to be coated in advance, feeder layer cells do not need to be used, the expression rate of the prepared NK cells CD3-CD16+/CD56+ reaches up to 90% or above after 14-16 days of culture, the in-vitro tumor killing activity is high, and the problems that in an existing NK culture system and technology, the purity of the NK cells is low, and the killing activity is poor can be solved.

The invention provides a kieselguhr insecticide, and a preparation method and application thereof. The kieselguhr insecticide comprises the following components in percentage by mass: 80-91 percent of kieselguhr powder, 1.5-3.5 percent of pyrethrum powder and 7.5-16.5 percent of sepiolite powder. The insecticide contains simple components, is high in insecticide efficiency, safe, low in toxicity and low in residue, and is an environment-friendly natural plant insecticide. The insecticide is extremely powerful in lethality to software insects, and can be widely applied to storage of grains and seeds and prevention and treatment of plant diseases and insect pests of crop fruit, vegetable and food production; meanwhile, the insecticide preparation method is simple and is suitable for industrial production.

The invention discloses a preparation method and application of water-soluble titanium dioxide nanoparticles. According to the preparation method, beta-cyclodextrin is used as an initial reaction material, a macroinitiator is synthesized by means of modification, a multi-arm star-shaped block copolymer is prepared by using an atom transfer radical polymerization (ATRP) technology and a technologywhich combines ATRP with 'click' chemistry, and the water-soluble titanium dioxide nanoparticles with uniform sizes and good dispersibility are prepared by taking the multi-arm star-shaped block copolymer as a monomolecular micelle template on the basis of a solution phase synthesis method. The titanium dioxide nanoparticles prepared by the preparation method are stable in quality, uniform in particle sizes, and can be stabilized at a nanoscale; the properties of the titanium dioxide nanoparticles can be effectively enabled to be exerted. The surfaces of the titanium dioxide nanoparticles aremodified, so that the titanium dioxide nanoparticles can be enabled to be evenly dispersed in water, and an aggregation effect is further avoided.

An automatically changing saw blade unit used as the obstacle in robot fight game to destroy the robot is composed of pneumatic control system, cylinder, piston and its connecting rod, motor, saw blade, rotary supporter and electric control system with electromagnetic valve fixed to said cylinder.

The invention relates to a preparation method of a nanometer preservative containing composite extracts and the corrosion resistance application of the nanometer preservative. According to the method disclosed by the invention, on the basis that composite clove /cinnamon /fresh ginger extracts (1:1-2:1-2) are extracted and compounded, the nanometer preservative containing the composite extracts is compounded, and the particle diameter can reach 8.69nm. The combination of the added 0.5g/kg of the nanometer preservative with the condition of disinfection for 15-20 minutes at the temperature of 85 DEG C can be applied to corrosion resistance and bacteriostasis of sauce products, so that the products can have the storage shelf life of one year. The nanometer preservative containing the composite clove /cinnamon /fresh ginger extracts, disclosed by the invention, has extensive bacteriostasis scopes, and has high safety, waster solubility and stability. The combination of the composite natural agent for corrosion resistance and bacteriostasis with flexible disinfection conditions can well maintain the nutrient substances and the flavor characteristics of products, and wide application prospects.

The invention discloses an intelligent remote-control solar insecticidal lamp which comprises a solar cell panel (1), a controller, an energy storage cell, a rain shade (2), a lamp post (9), a base (8), an energy-saving insecticidal lamp (4), a high voltage power network (3), an insect collector (5) and an insect collection box (7). The intelligent remote-control solar insecticidal lamp is characterized in that the intelligent remote-control solar insecticidal lamp further comprises a fault detection unit, a main control unit, a cell capacity detection unit, full collection warning units (6) for the insect collection box, a network transmission unit and a remote monitoring host computer, wherein the high voltage power network is arranged at the periphery of the energy-saving insecticidal lamp; the rain shade is arranged at the upper part of the energy-saving insecticidal lamp, and the lower part of the energy-saving insecticidal lamp is connected with the insect collector; the lower part of the insect collector is connected with the insect collection box; the controller, the fault detection unit, the main controller and the cell capacity detection unit are arranged below the insect collector; the full collection warning units (6) for the insect collection box are arranged at the inner part of the insect collection box; and the energy storage cell is arranged in the base. The intelligent remote-control solar insecticidal lamp has the advantages that the design is reasonable, and by additionally mounting remote monitoring control equipment, the working state of the insecticidal lamp can be remotely monitored.

The invention relates to a capsule for preventing and treating AIDS and a preparation technique thereof, pertaining to biomedical field, in particular to a capsule with high-esterified catechin as the main active ingredient thereof and a preparation technique of a micro-capsule of the high-esterified catechin as well. Microencapsulation enhances the stability of the high-esterified catechin and covers the unpleasant odor and taste. AIDS is one of the five main causations of death all over the world. The product of the invention is applied in the treatment of AIDS, performs a rather strong lethality on the AIDS, and can be substituted for the expensive and high-toxic imported drugs, which is suitable for national conditions of China, thereby having considerable economic benefits and promising market prospect.

The invention belongs to the field of targeted drug delivery systems, and particularly relates to a targeting thermosensitive liposome combination. The targeting thermosensitive liposome combination is characterized in that: the combination comprises thermosensitive liposome, the surface of which is modified by tumor homing peptide CREKA (cys-arg-glu-lys-ala) or peptide with a CREKA core sequence, and the thermosensitive liposome contains antitumor medicine. The invention also relates to a preparation method of the targeting thermosensitive liposome combination and an application of the targeting thermosensitive liposome combination in the preparation of drugs for treating malignant tumors. The targeting thermosensitive liposome has a good tumor-targeting effect and a good antitumor effect.

The invention discloses a drug for treating and/or preventing tumor. The drug comprises active components, namely a component A and a component B, wherein the component A is ascorbic acid soluble salt or ascorbic acid, and the component B is disulfiram. The compound drug which comprises sodium ascorbate and the disulfiram can effectively inhibit the growth of in-vivo in-vitro cancer cells, achieves the synergistic effect, achieves the selective killing effect on tumor cells and can be used as a low-toxicity effective compound drug for treating and/or preventing the tumor.

The invention relates to a povidone iodine five-compound disinfectant which comprises the following components in percentage by weight: 5-20 percent of povidone lodine, 0.1-10 percent of medical glycerin, 0.1-2 percent of citric acid, 0.1-1 percent of nonoxynol-10, 0.01-0.1 percent of sodium hydroxide and the balance of water for injection. The povidone iodine five-compound disinfectant can be used for producing povidone iodine compound wipes with different specifications, povidone iodine compound cotton swabs, povidone iodine compound spongy cushions, povidone iodine compound hand-washing brushes and the like; and after the products are specially treated or sterilized, the sterility and the safety of the products are fully ensured, and after being unpacked, the products can be directly used, so that the cross contamination and the infection in an intermediate link are avoided. The povidone iodine five-compound disinfectant has the advantages of small skin irritation, low toxicity, lasting action, and safety and convenience in use; the povidone lodine five-compound disinfectant basically has no irritation to tissues and is used for skin and mucosa disinfection, such as cleaning before operation, operative site and wound disinfection, and skin cleaning and disinfection before and after arterial blood drawing.

The invention relates to a biochemical pesticide mixed fatty acid preparation and its application. The mixed fatty acid preparation contains 1-30% mixed fatty acid and 70-99% auxiliary agents based on the total weight of the mixed fatty acid preparation; the auxiliary agents are surfactants, emulsifiers, antifreeze agents, dispersants and the like. The mixed fatty acid preparation of the present invention has an EC50 of 0.0479 mg/L for the tobacco virus disease, has strong lethality against the tobacco virus disease, and the control effect can reach more than 70% 14 days after administration. The mixed fatty acid preparation of the invention has the characteristics of high efficiency, low toxicity, low price and no environmental pollution, and has extremely broad market application prospects.

The invention relates to an embedded composite wallboard of an earthquake proof construction, comprising a shell, and a corrugated ribbed slab and packing which are arranged in the shell, wherein the shell is made of a glass magnalium composite board; the corrugated ribbed slab is made of aluminum plastic glass fiber reinforced plastics; the packing is made of the mixture of perlite powder, kieselguhr and magnesite powder; and the corrugated ribbed slab in the packing and the shell outside the packing are solidified together by using mucilage glue. Since the composite wallboard takes the light high-strength glass magnalium composite board as the shell, the light high-strength aluminum plastic glass fiber reinforced plastics corrugated ribbed slab as a reinforcing rib and the light, heat-insulation, sound-insulation and insulated mixture of the perlite powder, the kieselguhr and the magnesite powder as the packing, and the shell, the corrugated ribbed slab and the packing are solidified together by using the mucilage glue, the embedded composite wallboard has the properties of firmness and light weight and the characteristics of heat preservation, fire prevention and noise insulation. Especially when the embedded composite wallboard is put in use in a mode of small area and a building is damaged once an earthquake happens, the antipersonnel force to people can be reduced a lot, and therefore, the embedded composite wallboard is an ideal building material for the traditional earthquake proof construction.