57 results about "Thermosensitive liposomes" patented technology

A method and apparatus for controlling the safe delivery of thermosensitive liposomes containing medicant to a targeted tissue region using ultrasound. Thermosensitive liposomes containing medicants are delivered to a region of interest, the region of interest is located using ultrasound imaging, ultrasound therapy is applied to heat the region of interest, and the temperature of the region is monitored to determine whether a designated threshold temperature has been reached which allows for the release of medicants from the liposomes. If the threshold temperature is reached, and the liposomes are melted, the treatment stops. If the threshold temperature has not been reached, the application of ultrasound therapy and ultrasound imaging are alternated until the threshold temperature is reached. The ultrasound imaging, temperature monitoring and ultrasound therapy are preferably performed with a single transducer.

Thermosensitive liposomes encapsulating paramagnetic iron oxide nanoparticles are used as a drug controlled release system. Paramagnetic iron oxide nanoparticles are used to generate heat by applying alternative magnetic field to cause leakage of drugs in the liposomes.

The invention provides thermo-sensitive liposome. The thermo-sensitive liposome comprises a chemotherapeutic drug, a photosensitizer, phosphatidylcholine and polyethylene glycol-derived phospholipid, wherein the photosensitizer is indocyanine green or chlorins e6; the grain size of the thermo-sensitive liposome is 40-60 nm; by virtue of living-body fluorescence imaging, a distribution process of the thermo-sensitive liposome can be observed in real time; under a near-infrared light irradiation condition, the chemotherapeutic drug can be quickly and effectively released, so that the chemotherapeutic drug can be enriched at a target part very well; the obtained thermo-sensitive liposome particle has smaller particle diameter less than 100 nm, and good biocompatibility. The preparation method of the thermo-sensitive liposome is simple and easy to operate.

The present invention relates to supersonic focusing phase control array method to form large focus domain. Heat field shape is set based on the size and shape of the target tissue, and the supersonic focusing phase control array transducer has its element exciting signal phases and amplitudes regulated to generate various modes of multiple focus supersonic field distribution, so as to form required focus domain shape and size, provide supersonic energy for heating the target tissue and form intracorporeal local stereo heating region up to 30 mm. For even larger target tissue, the divisional synthesis mode is adopted. The formed multiple focus supersonic field may be exchanged or rotated to ensure homogeneous heat distribution. The present invention makes it possible to realize shaped directional heating of deep body target tissue, and this provides the technological foundation for the treating technology of directional opening of heat sensitive liposome and the heat synergistic effect of carried heat sensitive medicine.

The present application relates to thermosensitive liposomes encapsulating nanoparticles which can be used in the health sector, in particular in human health. The invention also relates to pharmaceutical and diagnostic compositions comprising thermosensitive liposomes as defined previously, as well as to their uses.

The invention discloses a thermosensitive liposome capable of conducting magnetic-heat release. The thermosensitive liposome consists of a phospholipid liposome which is constructed by a thermosensitive material and magnetic nanoparticles covered in the phospholipid liposome, wherein phase-transition temperature of the phospholipid liposome is at 40-51 DEG C; the magnetic nanoparticles are superparamagnetic ferriferrous oxide particles; the magnetic nanoparticles can become heated in a (high-frequency) alternating magnetic field, and the phospholipid liposome is heated up to the phase-transition temperature and substances in the phospholipid liposome are released; and near-infrared fluorescence quantum dots are also covered in the phospholipid liposome. The thermosensitive liposome provided by the invention, in which the magnetic nanoparticles are covered, has a magnetic-heat effect, and the thermosensitive liposome can achieve local heating to result in structural damage in the alternating magnetic field, so that effective ingredients in the thermosensitive liposome are released; the thermosensitive liposome has the characteristic of being high in controlled-release precision; and in the combination with the near-infrared fluorescence quantum dots which serve as a tracing ingredient, the problem that the magnetic thermosensitive liposome, when entering a body, is difficult to represent is effectively solved, and a tracing effect is achieved.

The invention discloses a nanometer cluster medicine carrying thermosensitive liposome preparation and a making method and application thereof. The preparation is obtained after impurity removal of nanometer cluster medicine carrying thermosensitive liposome, wherein the nanometer cluster medicine carrying thermosensitive liposome contains a thermosensitive phosphatide component DPPC, a phosphatide component MPPC capable of promoting release, a phosphatide component DSPE-PEG2000 capable of promoting long circulation of the preparation, and a tumor treating medicine; and the liposome is prepared by a film dispersion method, the surface of the liposome is wrapped with a layer of gold nanometer clusters, or the inner part of the liposome is encapsulated with magnetic Fe3O4 nanoparticles. Thepreparation can be used as an antitumor carrier, is connected with an antibody, and is specifically targeted to tumors, through NIR illumination, the medicine is efficiently released at the positionsof the tumors, and through combination with the sensitization effect of thermotherapy and radiotherapy, the treatment effects are improved. The preparation can also be combined with the advantages ofgold and ferrum in the imaging respect of electronic computer tomoscan imaging (CT)/magnetic resonance imaging (MRI) and the like, and can perform medical diagnosis, so that the purpose of achieving multimode pinpoint diagnosis and treatment of the tumor can be realized.

The invention provides a preparation method of a targeted temperature-controlled water-loading silibinin temperature-sensitive lipidosome-microbubble complex drug delivery system. The preparation method comprises the following steps of with single-palmitoyl phosphatidyl choline, dipalmitoyl phosphatidyl choline, distearoyl phosphatidyl ethanolamine-polyethylene glycol-amino and silibinin as raw materials, preparing water-loading silibinin temperature-sensitive lipidosome by virtue of a membrane rotary evaporation method; and coupling the water-loading silibinin temperature-sensitive lipidosome with an ultrasonic contrast agent, namely SonoVue microbubble, so as to prepare the targeted temperature-controlled water-loading silibinin temperature-sensitive lipidosome-microbubble complex drug delivery system. According to the preparation method, a new drug delivery system is established by combining the temperature-sensitive lipidosome with the microbubble; the efficient release of local tissues of a drug at a sub-high temperature field are achieved by virtue of the targeted explosion of the microbubble and the local temperature control effect of the temperature-sensitive lipidosome, so that the bioavailability of the drug is improved, and furthermore, residual tumors during thermal ablation treatment are reduced.

The invention is directed to, inter alia, injectable materials, which comprise a a zero-length cross-linking agent encapsulated in a thermo-responsive liposome dispersed in a polymer solution where the polymer and cross-linking agent are segregated at a first temperature, and non-segregated at a second temperature, and the material is injectable at the first temperature. The invention is directed to, inter alia, processes for producing the material and use of the material for tissue repair and regeneration and controlled delivery of an agent are also described.

The invention relates to a preparation method of a drug delivery system of an invisible thermosensitive liposome as well as an application of the drug delivery system of the invisible thermosensitive liposome in tumor treatment drugs, and can be used for effectively solving the problems that an existing tumor treatment drug is large in side effect, poor in treatment effect, and the like. The drug delivery system of the invisible thermosensitive liposome is composed of a thermosensitive liposome-loaded targeted heat sensitizing agent and chemotherapy drugs in a weight ratio of 1:3, wherein the invisible thermosensitive liposome is obtained through synthesis of carboxylic nanotubes, synthesis of folate-targeted carbon nano tube-lysine, preparation of the invisible thermosensitive liposome or synthesis of carboxylic nanotubes, synthesis of carbon nano tubes loaded with chemotherapy drugs, and preparation of the thermosensitive liposome. The preparation method disclosed by the invention is good in physical and chemical stability, simple and practicable in preparation condition, rich in material resources, low in cost, small in side effect and capable of effectively restraining multiplication of tumor cells, so that the effect of tumor-targeted treatment is achieved, and therefore, the preparation method is an innovation of a drug carrier in tumor-targeted treatment.

The invention relates to a thermosensitive liposome, which contains at least one phosphatidylcholine, at least one lysophospholipid, and at least one unsaturated phospholipid. The phase temperature of the liposome is 39.0DEG C-45.0DEG C. The liposome further contains a material having a long circulating characteristic and a fat-soluble active agent. Specifically, the phosphatidylcholine, the lysophospholipid, the unsaturated phospholipid, and the material having a long cycle characteristic are in a weight ratio of 69-94:1-6:1-10:4-15. And all the phospholipids and the fat-soluble active agent are in a weight ratio of 30-120:1-10. The invention also relates to a pharmaceutical composition containing the thermosensitive liposome. The invention additionally relates to application of the thermosensitive liposome and the pharmaceutical composition in preparing drugs treating tumors.

The invention belongs to the field of targeted drug delivery systems, and particularly relates to a targeting thermosensitive liposome combination. The targeting thermosensitive liposome combination is characterized in that: the combination comprises thermosensitive liposome, the surface of which is modified by tumor homing peptide CREKA (cys-arg-glu-lys-ala) or peptide with a CREKA core sequence, and the thermosensitive liposome contains antitumor medicine. The invention also relates to a preparation method of the targeting thermosensitive liposome combination and an application of the targeting thermosensitive liposome combination in the preparation of drugs for treating malignant tumors. The targeting thermosensitive liposome has a good tumor-targeting effect and a good antitumor effect.

The invention relates to preparation of thermosensitive liposome co-loaded with hollow gold nanoparticles and tumor therapeutic agents and triple integration application thereof. The preparation is mainly prepared from dipalmitoyl phosphatidylcholine (DPPC), hollow gold nanoparticles and therapeutic agents. A preparation method of the preparation comprises the following steps: preparing thermosensitive liposome via a conventional method, coating the thermosensitive liposome with the hollow gold nanoparticles by using a film extrusion method, and then coating the thermosensitive liposome with the therapeutic agents. The preparation is capable of reducing toxic and side effects of systemic drug therapy and is also capable of achieving the triple integration therapy effect with combination of drug therapy, thermal therapy and radiation therapy; the tumor killing effect is improved.

The invention discloses a targeted lipidosome with pH and temperature dual responsiveness. The targeted lipidosome comprises a lipidosome carrier and lipotropic medicine, wherein the lipidosome carrier is prepared from the following raw materials in parts by weight: 8 parts of dipalmitoyl phosphatidyl choline, 1-3 parts of hydrogenated soybean phosphatide, 0.5-2 parts of cholesterol-benzimide-polyethylene glycol, 0-1.5 parts of single-chain phospholipid, 0.01-0.1 part of cell penetrating peptide Stearyl-R8 and 0-3 parts of vitamin E. Polyethylene glycol and the cell penetrating peptide are adopted to jointly modify the lipidosome, trigger type active targeting is realized through pH responsiveness, the requirements on targeting performance and in-vivo long circulation are taken into consideration, meanwhile, a temperature-sensitive lipidosome is adopted to be serve as a membrane material, and the rapid release of the medicine can be realized through local thermal therapy.

The invention relates to a compound phospholipid thermosensitive lipidosome with an effect of simultaneously delivering drugs in double target areas, a preparation method and an application thereof. According to the invention, a folic acid modified cyclodextrin loaded drug is used for preparing an inclusion compound, and then the folic acid modified cyclodextrin inclusion compound and free drugs are respectively loaded into an inner water phase and a lipid bilayer of a compound phospholipid thermosensitive lipidosome; in an antitumor application, the free drugs and the drug-loading folic acid modified cyclodextrin inclusion compound are triggered to release from the lipidosome on a target part in the manner of heating; the free drugs mainly act on the tumor extracellular mesenchyme; the folic acid modified cyclodextrin inclusion compound enters tumor cells in the manner of receptor-mediated active targeting drug delivery; the simultaneous drug delivery in the tumor stroma and tumor cell double target areas is finally realized; the optimal therapeutic effect is realized in the manner of flexibly adjusting the drug ratio in the free drugs and the folic acid modified cyclodextrin inclusion compound. The invention is especially suitable for the antitumor application of complex component or monomer component of the traditional Chinese medicine with multiple effects and has wide application prospect in the aspect of research on a novel antitumor drug delivery system.

The present invention relates to a formulation of thermosensitive liposomes, and more specifically to a formulation of liposomes comprising phospholipids and a surface active agent, wherein the liposomes support long term storage at temperatures less than or equal to about 8° C., control degradate formation to maximize product potency and release their contents at mild hyperthermic temperatures. Methods of making formulations are also described.

The invention discloses a target temperature control type polysaccharide-supporting long-circulating liposome-microbubble compound drug delivery system and a preparation method thereof. Ganoderma applanatum polysaccharide-supporting thermosensitive liposome prepared with a compound emulsified solvent evaporation method and perfluoropropane microbubbles prepared with a film dispersion method are taken as examples, liposome and microbubbles are connected with a biotin-avidin coupling method, and a ganoderma applanatum polysaccharide-supporting thermosensitive liposome-microbubble compound is prepared. The compound is used as the drug delivery system, efficient release of drugs in sub-high-temperature field local tissue can be realized by use of targeted blasting of microbubbles and local temperature control effects, the bioavailability of drugs can be improved, and residues of thermal ablated tumor are reduced.

The invention relates to a preparation method of thermosensitive liposome with a whitening effect. The preparation method is characterized by comprising the following steps: A, weighing a whitening composition, liposome, a membrane stabilizer, ethyl alcohol, glycerol and deionized water; B, adding the liposome and the membrane stabilizer into the ethyl alcohol, stirring under the condition that the temperature is 20 to 25 DEG C and the rotating speed is 300 to 800 rpm till a mixture is completely dissolved; adding the glycerol and continuously stirring till the liquid becomes clear and transparent to obtain a lipid membrane mixed solution; C, adding the whitening composition into the lipid membrane mixed solution and stirring under the condition that the temperature is 20 to 25 DEG C and the rotating speed is 300 to 800 rpm till the liquid becomes clear and transparent to obtain a whitening agent and lipid membrane pre-mixed solution; D, adding the whitening agent and lipid membrane pre-mixed solution into the deionized water, stirring for 10 minutes under the condition that the temperature is 20 to 25 DEG C and the rotating speed is 300 to 800 rpm, putting into a refrigerator andreducing the temperature to 2 to 5 DEG C; then at the pressure of 1500 bar, homogenizing for 1 to 3 times by using a high pressure homogenizer, thus obtaining the thermosensitive liposome with the whitening effect. The thermosensitive liposome with the whitening effect, disclosed by the invention, has the beneficial effects of simultaneous loading of three whitening active substances, good whitening effect and thermosensitive characteristic after transdermal absorption.

The invention relates to a nano copper sulfide based active-targeting thermo-sensitive liposome preparation method and application. The liposome is prepared from tumor targeting molecules, copper sulfide nano particles and a chemotherapeutic medicine, wherein the tumor targeting molecules refer to GA (glycyrrhetinic acid), the copper sulfide nano particles are hydrophilic copper sulfide nano particles obtained after povidone modification, and the chemotherapeutic medicine refers to docetaxel. The preparation method includes steps: synthesizing carbenoxolone octadecyl ester; preparing active-targeting thermo-sensitive liposome. The nano copper sulfide based active-targeting thermo-sensitive liposome prepared according to the method is applied to preparation of tumor targeted medicines. By adoption of the tumor targeting molecules as targeting heads, integration of tumor hyperthermia chemotherapy and targeted therapy is realized according to copper sulfide photothermal conversion characteristics. The method is stable, reliable, convenient to use, effective and easy in operation, and rich raw material sources, low cost, low side effects, effectiveness in inhibition of tumor cell proliferation and innovation on medicines for tumor targeted therapy are realized.