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Preparation method of invisible thermosensitive liposome as well as application of drug delivery system of invisible thermosensitive liposome in tumor treatment drugs

An invisible heat-sensitive lipid and heat-sensitive liposome technology, which can be used in anti-tumor drugs, liposome delivery, drug combination and other directions, can solve problems such as large side effects and poor treatment effects, and achieve low side effects and preparation conditions. Simple and feasible, the effect of rich source of raw materials

Inactive Publication Date: 2014-01-22
ZHENGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In view of the above situation, to overcome the defects of the prior art, the purpose of the present invention is to provide a preparation method of a stealth thermosensitive liposome drug delivery system and its application in tumor therapy drugs, which can effectively solve the side effects of existing tumor therapy drugs Large, poor treatment effect and other problems

Method used

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Embodiment 1

[0020] The preparation method of the present invention can be realized by the following steps in concrete implementation:

[0021] (1) Synthesis of carboxylated carbon nanotubes: Weigh 100mg of carbon nanotubes, put them into a 250mL round bottom flask, add 120mL of mixed acid, 12mL of hydrogen peroxide solution with a mass concentration of 30%, and put them in an ultrasonic cleaner for 1 hour. Add 1L of ultrapure water to dilute, filter with 0.22 μm microporous membrane and Buchner funnel, rinse with ultrapure water until the pH is neutral, put it in an oven and dry at a constant temperature of 80°C to obtain carboxylated carbon nanotubes (CNTs- COOH); the mixed acid is a mixed acid composed of concentrated sulfuric acid and concentrated nitric acid in a volume ratio of 3:1;

[0022] (2) Synthesis of folic acid-targeted carbon nanotubes-lysine: Weigh 50 mg of carboxylated carbon nanotubes, add 25 mL of 0.2 M lysine solution, then add 10 mg of folic acid, 50 mg of 1-ethyl-(3- ...

Embodiment 2

[0025] The preparation method of the present invention can also be realized by the following steps in specific implementation:

[0026](1) Synthesis of carboxylated carbon nanotubes: Weigh 105 mg of carbon nanotubes, put them into a 250 mL round bottom flask, add 120 mL of mixed acid, 13 mL of hydrogen peroxide solution with a mass concentration of 30%, and sonicate in an ultrasonic cleaner for 1 hour. Add 1L of ultrapure water to dilute, filter with 0.22 μm microporous membrane and Buchner funnel, rinse with ultrapure water until the pH is neutral, put it in an oven and dry at a constant temperature of 80°C to obtain carboxylated carbon nanotubes (CNTs- COOH);

[0027] (2) Synthesis of folic acid-targeted carbon nanotubes-lysine: Weigh 53 mg of carboxylated carbon nanotubes, add 25 mL of 0.2 M lysine solution, then add 11 mg of folic acid, 53 mg of 1-ethyl-(3- Dimethylaminopropyl) carbodiimide hydrochloride (EDC·HCl), 11mg N-hydroxysuccinimide (NHS), placed in a flask, after...

Embodiment 3

[0030] The preparation method of the present invention can be realized by the following steps in concrete implementation:

[0031] (1) Synthesis of carboxylated carbon nanotubes: Weigh 100mg of carbon nanotubes, put them into a 250mL round bottom flask, add 120mL of mixed acid, 12mL of hydrogen peroxide solution with a mass concentration of 30%, and after ultrasonic cleaning for 1h, add Dilute with 1L of ultrapure water, filter with 0.22μm microporous membrane and Buchner funnel, rinse with ultrapure water until the pH is neutral, and dry in an oven at 80°C to obtain carboxylated carbon nanotubes (CNTs-COOH) ;

[0032] (2) Synthesis of carbon nanotubes loaded with doxorubicin hydrochloride-lysine: Weigh 50 mg of carboxylated carbon nanotubes, add 25 mL of 0.2M lysine solution, 50 mg of 1-ethyl-(3-dimethylaminopropyl base) carbodiimide hydrochloride (EDC·HCl) and 10 mg N-hydroxysuccinimide (NHS), placed in a flask, ultrasonically dispersed for 2 hours, then stirred at 100 r / mi...

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Abstract

The invention relates to a preparation method of a drug delivery system of an invisible thermosensitive liposome as well as an application of the drug delivery system of the invisible thermosensitive liposome in tumor treatment drugs, and can be used for effectively solving the problems that an existing tumor treatment drug is large in side effect, poor in treatment effect, and the like. The drug delivery system of the invisible thermosensitive liposome is composed of a thermosensitive liposome-loaded targeted heat sensitizing agent and chemotherapy drugs in a weight ratio of 1:3, wherein the invisible thermosensitive liposome is obtained through synthesis of carboxylic nanotubes, synthesis of folate-targeted carbon nano tube-lysine, preparation of the invisible thermosensitive liposome or synthesis of carboxylic nanotubes, synthesis of carbon nano tubes loaded with chemotherapy drugs, and preparation of the thermosensitive liposome. The preparation method disclosed by the invention is good in physical and chemical stability, simple and practicable in preparation condition, rich in material resources, low in cost, small in side effect and capable of effectively restraining multiplication of tumor cells, so that the effect of tumor-targeted treatment is achieved, and therefore, the preparation method is an innovation of a drug carrier in tumor-targeted treatment.

Description

technical field [0001] The invention relates to the field of drugs, in particular to the preparation of a stealth thermosensitive liposome and the application of the drug delivery system in tumor treatment drugs. Background technique [0002] Stealth thermosensitive liposome is a physicochemical and active targeting drug delivery vehicle. At normal body temperature, it is difficult for hydrophilic drugs to diffuse out through the liposome membrane, and when the liposome passes through the heated target organ along with the blood circulation, the local high temperature can promote the release of the drug at the target site and form a relatively tight liposome. High drug concentration; Stealth thermosensitive liposome has good drug loading, targeting and biocompatibility, and has become a research hotspot in the field of biomedicine. It has been reported that the local heating methods of thermosensitive liposomes include water bath method, Microwave method, radio frequency me...

Claims

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Application Information

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IPC IPC(8): A61K45/06A61K41/00A61K9/127A61K47/48A61P35/00A61K31/704A61K31/337
Inventor 祝侠丽张振中谢莹霞黄胜楠侯琳张慧娟王蕾史进进张英杰黄河清
Owner ZHENGZHOU UNIV
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