31 results about "Liposome membrane" patented technology

The invention discloses an irinotecan and afatinib maleate co-loaded liposome preparation as well as a preparation method and application thereof, irinotecan and afatinib maleate are taken as effective components of the liposome, and the liposome comprises a liposome carrier, an inner water phase positioned in a liposome membrane and an outer water phase positioned outside the liposome membrane; the irinotecan and the afatinib maleate are encapsulated in the inner water phase; the inner water phase comprises an ammonium salt aqueous solution, and an ammonium salt gradient exists between the inner water phase in the liposome membrane and the outer water phase outside the liposome membrane; the outer water phase is a physiological isotonic solution. According to the present invention, the research results show that the irinotecan and afatinib maleate co-carrying liposome can simultaneously deliver the two drugs with the synergistic ratio into the same tumor cell compared to the irinotecan single drug liposome and the afatinib maleate single drug liposome so as to maximize the synergistic effect of the drugs, and significantly improve the anti-tumor effect;

The application provides a paclitaxel liposome injection and a preparation method thereof, and belongs to the technical field of pharmaceutical preparations. The injection is prepared from the following injection-grade raw and auxiliary materials: paclitaxel, phospholipid, cholesterol, gamma-aminobutyric acid, potassium lactate, a buffer, a freeze-drying protective agent and water for injection. The gamma-aminobutyric acid and the potassium lactate are simultaneously added as the liposome membrane stabilizer, the two synergistically act, the compactness and the stability of the lipid bilayer are significantly enhanced, the paclitaxel encapsulation rate is improved, the drug leakage rate is reduced, and the long-term storage stability of the preparation is improved.

The invention discloses a cholesterol modified double-stranded DNA membrane skeleton liposome delivery system and a preparation method and application thereof, and belongs to the technical field of drug delivery. The system is composed of cholesterol, distearoyl phosphatidylcholine, distearoyl phosphatidyl ethanolamine modified by methoxy polyethylene glycol and double-stranded DNA (chol-dsDNA) modified by 5 '-cholesterol, and the chol-dsDNA is anchored on the inner side and the outer side of a liposome membrane through a hydrophobic effect to form a bionic skeleton. The system is excellent in mechanical stability, low in drug leakage rate, high in tumor targeted enrichment capacity and good in biocompatibility, can efficiently entrap irinotecan hydrochloride and other hydrophilic drugs, is used for tumor treatment, and is simple and convenient to prepare and easy to scale.

The invention discloses a multifunctional double-drug delivery system based on metal collaborative treatment and application of the multifunctional double-drug delivery system, and belongs to the technical field of biological medicine. The double-drug delivery system is double-drug delivery lipidosome and comprises metal ions, a lipidosome membrane and active ingredients, the metal ions are adsorbed on the surface of the lipidosome membrane, and the active ingredients are wrapped in the lipidosome membrane; the metal ions are divalent metal ions; the active component consists of an STING agonist and a traditional Chinese medicine active monomer component. According to the double-drug delivery system, the STING agonist and the traditional Chinese medicine anti-inflammatory components are co-loaded through lipidosome, and multi-dimensional remodeling of a tumor microenvironment is achieved through the double effects of exciting tumor immunity and inhibiting inflammatory reaction; and meanwhile, by introducing metal ions, the lysosome escape efficiency of a liposome drug delivery system is effectively enhanced, so that the intracellular delivery efficiency of the drug is improved.

Tolerogenic liposome-based compositions and uses thereof for treating immune disorders. The compositions include two populations of liposomes. The first population of liposomes has a size in the range from 2 to 200 nm, and the second population of liposomes has a size in the range from 500 to 2000 nm. The liposomes of the first and second populations carry one or more antigens. The liposomal membrane of each liposome in the first and second liposome populations include phosphatidylserine in an amount ranging from 20 to 60% by weight with respect to the total composition of the liposome's membrane.

The invention discloses a chitosan modified turmeric-broccoli exosome hybrid nasal spray and application thereof, and belongs to the technical field of biological medicine and drug delivery. The nasal spray is formed by dispersing double plant exosome-lipidosome hybrids in a carrier for nasal cavities. The preparation method comprises the following steps: fusing curcumin carried by a turmeric exosome and sulforaphane carried by a broccoli bud exosome with a pH response liposome membrane containing 1, 2-dioleoyl-sn-glycerol-3-phosphoethanolamine, and modifying by chitosan to form a three-layer structure; the preparation method comprises the steps of exosome extraction, ultrasonic drug loading, liposome hybridization, chitosan coating and preparation blending. The nasal spray is high in entrapment efficiency and uniform in particle size, the chitosan enhances nasal adhesion, inflammation targeted drug release is achieved through pH response, the brain targeting efficiency and stability are excellent, and the adaptability to children is good. The curcumin and sulforaphane are used for targeted therapy of children autism spectrum disorder neuroinflammation, anti-inflammation and anti-oxidation are achieved through synergism of curcumin and sulforaphane, the curative effect is remarkable, and a new scheme is provided for treatment of related diseases.

The invention relates to a process for obtaining an amphotericin B (AmB) formulation in liposomes containing dicetyl phosphate (DCP), based on incorporation of the drug into the membrane of preformed liposomes, combining the effects of pH on the solubility of amphotericin B and of temperature on the insertion of the drug into the liposome membrane. The technology also relates to use of the process and of the formulation for the manufacture of medicaments for the treatment of leishmaniasis and sporotrichosis, for oral or topical administration.

Disclosed herein is a biomimetic torpedo that can circumvent existing hurdles for RNA therapies. That is, RNA therapeutics can be harbored inside a torpedo shell made of neutrophil membranes in hybrid with a liposome membrane, which enables lesion targeting and shielding from immunogenicity. Further disclosed herein is a biomimetic targeting system that involves a neutrophil cell membrane capsule that encapsulates a therapeutic RNA.

This invention belongs to the field of ultrasound contrast microbubble synthesis technology, and relates to a method for preparing and applying ultrasound contrast microbubbles targeting CD93 molecules in tumor vascular endothelium. The method includes the following steps: constructing a recombinant expression vector capable of expressing the CD93 ligand MMRN2 protein, transfecting cells, and extracting the cell membrane using differential centrifugation; mixing dipalmitoylphosphatidylcholine, distearate phosphatidylethanolamine-polyethylene glycol 2000, and chloroform, adding DiI, and preparing a liposome membrane using a thin-film hydration method; mixing the cell membrane and the liposome membrane, performing ice-bath sonication, and mixing and shaking with perfluoropropane to obtain an ultrasound contrast agent targeting CD93 molecules in tumor vascular endothelium. The ultrasound contrast microbubbles prepared by the method of this invention have good biocompatibility compared to conventional antibody-targeting strategies, and can also improve stability and targeting, thereby enabling the assessment of CD93 molecule expression in tumors.

The application belongs to the field of biological medicine, and discloses a nano-lipid preparation for loading perfluorohexane and an antioxidant, wherein the nano-lipid preparation is a core-shell structure, the core-shell structure takes a phospholipid liposome membrane layer as an outer shell, and perfluorohexane and the antioxidant loaded in the outer shell serve as an inner core; the phospholipid liposome membrane layer is made of hydrogenated lecithin, distearoyl phosphatidyl ethanolamine-polyethylene glycol 2000 and cholesterol; and the application discloses an application of the nano-lipid preparation for loading perfluorohexane and the antioxidant in preparation of a medicine for treating myocardial infarction. The nano-lipid preparation has a suitable particle size, can be passively targeted and enriched to a myocardial infarction site, is stable, has a high encapsulation efficiency, and has a simple administration mode; after the lipid preparation is taken by myocardial cells, oxygen and the antioxidant are released, the oxygen can relieve an anoxic condition of a myocardial ischemia site, and the antioxidant can reduce ROS damage, both of which play a synergistic role, improve the survival rate of ischemic myocardial cells, and improve heart function.

The invention belongs to the technical field of biological detection, and relates to a synchronous detection sensor for small extracellular vesicle membrane proteins PD-L1 and miRNA-21 based on aptamer mediated membrane fusion and application of the synchronous detection sensor. The sensor comprises (1) a multifunctional liposome, a hairpin probe H1 and a hairpin probe H2 are encapsulated in the liposome, the surface of a liposome membrane is modified with an EpCAM nucleic acid aptamer and an auxiliary chain, and miRNA-21 in small extracellular vesicles, the hairpin probe H1 and the hairpin probe H2 form a first catalytic hairpin self-assembly system; (2) AptPD-L1-L with a hairpin structure, the AptPD-L1-L comprises a PD-L1 nucleic acid aptamer, a connecting arm, an auxiliary chain hybridization region and a partial excitation chain sequence which are sequentially connected from the 5'end to the 3 'end, and the partial excitation chain sequence and the 5' end of the auxiliary chain jointly form an excitation chain; and (3) a hairpin probe H3, a hairpin probe H4, the AptPD-L1-L, the auxiliary chain, the hairpin probe H3 and the hairpin probe H4 form a second catalytic hairpin self-assembly system. According to the invention, synchronous detection of miRNA-21 and membrane protein PD-L1 in sEVs is realized, and a new technical platform is provided for lung cancer liquid biopsy.

The invention discloses a silybum marianum lipidosome applied to a liver protection tablet product and a preparation method, the silybum marianum lipidosome comprises a core material, and an internal wall material and an external wall material which sequentially wrap the surface of the core material; the core material is a silybum marianum extract; the inner wall material is modified soybean phospholipid; the outer wall material is a combination of starch sodium octenylsuccinate and polyethylene glycol. A lipidosome membrane embedding technology is adopted, the silybum marianum extract and soybean modified phospholipid are mixed according to a certain proportion and emulsified into a membrane, phospholipid bimolecular membrane embedding is formed, and then high-molecular compounds including starch sodium octenylsuccinate and polyethylene glycol are used as external wall materials to further include the phospholipid compound of the silybum marianum extract. The prepared lipidosome can be uniformly dispersed in an aqueous solution, the angle of repose is less than or equal to 40 degrees, the fluidity and the tabletting effect are better, the lipidosome is prepared into a sustained-release tablet, the bioavailability of the lipidosome can be improved, and the application of the lipidosome in a liver protection factor system can be expanded.

The preparation method comprises the following steps: modifying lysine-proline-valine on phosphatidylserine active ester to obtain an anionic liposome, forming a liposome membrane by using the anionic liposome and cholesterol, and then uniformly coating a layer of liposome on the outer layer of each probiotic cell by using calcium ion mediation to realize single cell coating, so that a single cell is obtained; a probiotic oral delivery system is obtained. According to the probiotic oral delivery system, coating of probiotic single cells can be achieved, and meanwhile the survival rate of the probiotics is not affected by a coating method in the coating process. In addition, the probiotic oral delivery system provided by the invention can effectively improve the viability of the probiotics in gastrointestinal fluid, increase colonization of the probiotics in intestinal tracts, target inflammation parts in the intestinal tracts, effectively reverse DSS-induced colon injury, reduce inflammatory cell infiltration and recover crypts and mucous membrane layers; the expression of proinflammatory factors is obviously reduced, and the expression of anti-inflammatory factors is improved.

The invention provides a membrane phospholipid composition specific liposome for promoting eggshell mineralization and application thereof. The liposome membrane is prepared from the following components as raw materials: (a) core membrane phospholipid; (b) an acidic phospholipid and (c) a membrane stabilizing component; wherein the core membrane phospholipid comprises PC (Polycarbonate) and PE (Polyethylene); the acidic phospholipid is PS and / or PA; the membrane stabilizing component comprises at least one of methoxy polyethylene glycol modified phosphatidyl ethanolamine (DSPE-PEG), 1, 2-dimyristoyl-rac-glycerol-3-methoxy polyethylene glycol (DMG-PEG), 1, 2-dioleoyl-sn-glycerol-3-phosphoethanolamine-N-[methoxy (polyethylene glycol)] (DOPE-PEG), and cholesterol (Cholesterol). Through specific combination of core membrane phospholipids (PC and PE) and acidic phospholipids (PS and / or PA), an initial mineralization microenvironment in a living body is successfully simulated. In-vitro mineralization test results show that the liposome can effectively promote formation and growth of calcium carbonate crystal nucleuses, has excellent performance on interfaces of eggshell membranes, silicon wafers and the like, and proves universality and effectiveness.

The invention discloses a preparation method and anti-tumor application of a cantharidin-loaded ginsenoside nano-liposome, relates to the technical field of medicines, and in particular relates to a liposome which comprises phospholipid, ginsenoside and cantharidin. Ginsenoside and phospholipid are used as liposome membrane materials to wrap cantharidin, and the anti-tumor effect of the nano-liposome is improved. According to the preparation method disclosed by the invention, the nano-liposome particles with high entrapment efficiency are successfully obtained, the plaque and the ginsenoside are creatively combined for medication, and meanwhile, the use of cholesterol is reduced, so that the adverse stimulation reaction of the cholesterol to a human body is avoided.

The application discloses a general allogeneic CAR-NK cell preparation and application thereof, and belongs to the technical field of cell immunotherapy biological preparations. The preparation comprises pre-activated modified allogeneic CAR-NK cells, a composite protective agent system and a medicinal buffer, and is prepared from umbilical cord blood-derived NK cells through non-integrated CAR lentivirus transfection, cytokine combination pre-activation and liposome membrane anchoring HLA-G immune shielding modification. The composite protective agent system is created for the first time, and can simultaneously realize the functions of cryopreservation protection, in-vivo synergism and immunoregulation, and solve the defects of existing allogeneic CAR-NK preparations, such as dependence on gene editing, poor cryopreservation stability, high immunogenicity, short in-vivo survival time and insufficient anti-tumor activity, and can be used for preparing a therapeutic drug for CD19-positive hematological malignancies, and is suitable for industrialized development of "off-the-shelf" general cell treatment products.

The invention discloses a photoresponse drug delivery system and a preparation method and application thereof. The photoresponse drug delivery carrier comprises a photosensitive liposome and a metal polyphenol coordination polymer micelle, the metal polyphenol coordination polymer micelle comprises a metal polyphenol network coating and a polymer micelle, the surface of the polymer micelle is coated with the metal polyphenol network coating, and the metal polyphenol network coating is coated with the polymer micelle. The metal polyphenol coordination polymer micelle is loaded on the surface of the photosensitive liposome; the photosensitive lipidosome comprises a lipidosome membrane and an inner water phase located in the lipidosome membrane, the inner water phase comprises EDTA and ammonium salt, and the lipidosome membrane comprises a lipid material and a photosensitizer. The photoresponse drug delivery system has an efficient photoresponse drug release rate, sequential release of drugs can be achieved, and multi-mode combined anti-tumor is achieved through combination of photodynamic and chemotherapeutic drugs.

An irinotecan liposome preparation, a preparation method, and use thereof. The irinotecan liposome includes: irinotecan, a liposome carrier, an internal aqueous phase located inside a liposome membrane and an external aqueous phase located outside the liposome membrane, the irinotecan being encapsulated in the internal aqueous phase; a sulfonate gradient exists between the internal aqueous phases inside the liposome membrane and the external aqueous phase outside the liposome membrane. The liposome takes monovalent sulfonate and disulfonate as the internal aqueous phases, encapsulates the irinotecan in the internal aqueous phases of the liposome in the form of insoluble sulfonate or disulfonate, and has a good sustained-release effect.

The invention discloses an extracellular vesicle biomarker detection method based on anion liposome membrane fusion and enzyme-free amplification, and belongs to the technical field of biomedicine nanotechnology. According to the method, multi-index direct detection of biomarkers (including micro ribonucleic acid and microRNA) in extracellular vesicles (EVs) can be realized by a one-step method through the anionic liposome, a complicated pretreatment process is not needed, and the method can be used for noninvasive early detection, real-time monitoring and therapeutic response evaluation. By optimizing the preparation method and detection conditions of the anion liposome, the optimized detection method is finally obtained, the complex process of extracellular vesicle extraction and internal microRNA release is avoided, the loss and caused errors in the process are reduced, and the detection sensitivity and accuracy are improved. The detection method provided by the invention can realize multi-index parallel detection, has high accuracy, can meet clinical application requirements, and can provide a new thought and technical scheme for detection of extracellular vesicle biomarkers of diseases such as tumors.

The invention discloses a photoresponse type titanium dioxide vitamin E succinate nanoparticle and a preparation method and application thereof.The photoresponse type titanium dioxide vitamin E succinate nanoparticle introduces a photoresponse mechanism by co-loading DOX and TiO2NPs to achieve controllable drug release, meanwhile, alpha-TOS is introduced into a liposome component to optimize the physicochemical property of a liposome membrane, meanwhile, the cytotoxicity of a drug is synergistically enhanced, and the drug release effect is improved. The photocatalytic effect of the TiO2NPs is activated by illumination, reactive oxygen species (ROS) is generated in situ to accelerate the rupture of a liposome membrane, and efficient release of drugs in a tumor microenvironment is realized; the preparation method adopts an ultrasonication method to obtain liposome nanoparticles with small particle size (50nm-150nm) and high encapsulation efficiency (greater than 60%), and the liposome nanoparticles have the characteristics of long circulation stability and targeted controlled drug release, can effectively improve the tumor curative effect and reduce the system toxicity, and provides a novel drug delivery platform for combined photocatalytic treatment and chemotherapy.

The invention provides a preparation method and application of a fluorinated linear polypropylene imine loaded lipidosome. A dipalmitoyl phosphatidylcholine (DPPC)-cholesterol liposome membrane is adopted to coat terminal group fluorinated linear polyethyleneimine (F-PPI), and the particle size and stability of the liposome are improved by adjusting hydration conditions and a post-treatment mode, so that the liposome with excellent antibacterial performance, small cytotoxicity and good biocompatibility is obtained. The fluorinated polycation (DPPC / F-PPI) antibacterial agent is coated with the lipidosome with high biocompatibility, so that the bioavailability of the fluorinated linear polypropylene imine is improved; the compound has a wide application prospect in preparation of antibacterial and anti-infection drugs.

The invention discloses a methylprednisolone-loaded cell membrane bionic liposome atomization preparation. The atomization preparation comprises methylprednisolone-loaded cell membrane bionic liposome, a liposome membrane stabilizer and a buffer solution of a pH regulator, the invention also discloses a preparation method of the methylprednisolone-loaded cell membrane bionic liposome atomization preparation. The invention also discloses an application of the methylprednisolone-loaded cell membrane bionic liposome atomized preparation in preparation of drugs for treating acute lung injury or asthma, and an application of the methylprednisolone-loaded cell membrane bionic liposome atomized preparation as a carrier of targeted drugs in preparation of drugs for treating acute lung injury. The atomization preparation provided by the invention can realize local drug delivery so as to realize pulmonary drug delivery, and the drug action efficiency is improved.

The invention discloses an ilicin A-based blank liposome, an ilicin A-based drug-loaded liposome, and a preparation method and an application of the ilicin A-based blank liposome and drug-loaded liposome. The blank lipidosome comprises a lipidosome membrane and a cavity formed by the lipidosome membrane, and the lipidosome membrane comprises phospholipid and ilicin A. The drug-loaded liposome comprises a drug active component and a blank liposome, and the drug active component is entrapped in a liposome membrane and / or a cavity of the blank liposome. The blank liposome is uniform in size and has good stability and targeting property; after the drug active ingredients are entrapped, the stability and the targeting property of the drug can still be effectively improved.

The invention provides a liposome co-loaded with soybean meal polypeptide and baicalein as well as a preparation method and application thereof. The liposome co-loaded with soybean meal polypeptide and baicalein comprises a liposome, baicalein entrapped on a phospholipid layer of a hydrophobic layer of the liposome, a hydrophilic core and soybean meal polypeptide on the surface of the liposome. Firstly, a liposome membrane material and baicalein are assembled through a reverse evaporation method, then baicalein liposome and soybean meal polypeptide are assembled through an ultrasonic dispersion method, and the liposome co-loaded with soybean meal polypeptide and baicalein is prepared. According to the invention, a liposome drug loading technology is adopted, so that the two drugs can reach a pharmacological site sufficiently on the premise of ensuring the stability and the safety, and the synergistic effect of the two drugs in vivo is realized.

The invention discloses an application of ginsenoside liposome gel in tumor treatment. According to the drug system, ginsenoside Rg3 is used for replacing cholesterol to serve as a liposome membrane material, lipidosome is prepared by combining with lecithin through a membrane hydration method, and a chemotherapeutic drug cis-platinum is carried to form Rg3amp; cis (at) Liposomes, and Cis (at) Liposomes; further, the Rg3amp is entrapped in chitosan-beta-sodium glycerophosphate-gelatin temperature-sensitive hydrogel, and injectable Rg3amp is constructed; and Cis (at) Lipogel. The system realizes stable and synchronous delivery of drugs through peritumoral / local injection, inhibits IDO1-KYN-AhR metabolism-immune checkpoint axis in a targeted manner, activates a cGAS-STNG-IFN-I immune signal pathway, enhances cellular immunity, and has a remarkable inhibition effect on bladder cancer, triple negative breast cancer and melanoma.

The invention relates to the technical field of protein separation and extraction, and provides a high-purity ovotransferrin separation and extraction method. According to the method, the temperature response type phosphatidylserine lipidosome compound is used for adsorbing and separating the ovotransferrin. According to the temperature response type phosphatidylserine liposome compound, the temperature response characteristic of poly (N-isopropylacrylamide) is utilized, at the temperature of 25 DEG C, a PNIPAM chain is in a stretched state, phosphatidylserine is fully exposed and can be fully combined with ovotransferrin through electrostatic and hydrophobic effects, and the adsorption effect of phosphatidylserine is greatly enhanced; when the temperature rises to 37 DEG C, the PNIPAM chain shrinks, the pore size of the liposome membrane is reduced, the adsorbed ovotransferrin can be wrapped and fixed, and the ovotransferrin is prevented from falling off in the subsequent centrifugal process. According to the adsorption and fixation mechanism under temperature regulation and control, the activity of the ovotransferrin is well protected, and an efficient and reliable method for separating and extracting the high-purity ovotransferrin is provided.

The application discloses a fine-line anti-wrinkle composite peptide flexible liposome and a preparation method thereof. The fine-line anti-wrinkle composite peptide flexible liposome is composed of the following components: acetylcholine blocking polypeptide, liposome lipid material, liposome membrane softener, solvent (dissolving lipid material), antioxidant, stabilizer, preservative and water. The flexible liposome has good deformability, so that the wrapped active ingredients can penetrate the skin to reach the target area. The palmitic acid modified polypeptide hydrophobic long chain and the carboxyl and amino hydrophilic groups on the side chain are used to form a three-dimensional network hydrogel through intermolecular and intramolecular hydrogen bonds, so that the stability is improved. The propylene glycol can more effectively dissolve phospholipids, so that the cost is reduced, and the moisturizing and transdermal penetration enhancer effects are achieved. The application can be mixed with water in any proportion, is convenient to mix, and can be widely used in facial, neck and whole body skin care products, such as essence water, essence liquid, essence milk, essence cream and jelly, and has significant fine-line anti-wrinkle effects.

The invention discloses bone cement loaded with a bisphosphate modified nano iron oxide diagnosis and treatment integrated probe as well as a preparation method and application of the bone cement. The bone cement is composed of a calcium phosphate bone cement matrix and diagnosis and treatment integrated probes uniformly dispersed in the calcium phosphate bone cement matrix. The probe takes superparamagnetic ferroferric oxide nanoparticles as a core, a bone targeting function is realized by connecting diphosphate molecules through surface coordination, and the outer layer is coated with a liposome membrane to enhance biocompatibility and prolong cycle time and is used as a drug carrier. The bone cement disclosed by the invention has excellent mechanical properties, biological activity and multi-mode diagnosis and treatment functions; on one hand, the bone cement can be used as a bone defect filling material to provide mechanical support and promote bone regeneration; and on the other hand, the nano iron oxide in the probe can be used as a magnetic resonance imaging contrast agent, the diphosphate modification endows the probe with natural targeting ability to bone tissues and a curative effect of inhibiting osteoclasts, the liposome encapsulation realizes long-acting slow release and controllable release of the drug, and the systemic toxicity is reduced.

The application belongs to the technical field of biological materials, and provides a preparation method of a phytosterol-stabilized liposome carrier for delivering a nutritional factor, comprising the following steps: dissolving phospholipid, phytosterol and the nutritional factor in a poor solvent, rotating and evaporating the obtained mixture under vacuum until the poor solvent is completely removed, and forming a uniform phytosterol-liposome film for delivering the nutritional factor; hydrating the liposome film with a phosphate buffer solution containing TwEEn-80 to form a crude liposome; and performing low-temperature water bath ultrasonic treatment on the crude liposome carrier loaded with the nutritional factor, so as to obtain the phytosterol-liposome carrier for delivering the nutritional factor. The phytosterol-liposome carrier for loading the nutritional factor provided by the application uses phytosterol instead of cholesterol as a membrane stabilizer, can improve the stability and bioavailability of the nutritional factor in the gastrointestinal environment while loading the nutritional factor, and avoids the adverse effects of excessive intake of cholesterol on the human body.

The application discloses a kind of lees extract liposome and its preparation method and purposes.The lees extract liposome, including liposome membrane and the lees extract solution and polysaccharide enclosed in the liposome membrane, the raw material of the liposome membrane includes phospholipid, cholesterol and active protein;The active protein is selected from one or two of lees alcohol-soluble protein and rice protein;The polysaccharide is selected from one or two of arabinoxylan and beta-glucan.The lees extract liposome of the application can effectively load active ingredients in lees extract solution by constructing stable phospholipid bilayer structure, not only can protect active substances from oxidative degradation, but also can improve its transdermal diffusion rate, so as to enhance skin repair and function improvement effect.In addition, the lees extract liposome of the application can significantly regulate skin tactile friction behavior, so that the application process is more smooth, reduces adhesion, significantly improves experience.