Polyglutamic acid and human epidermal growth factor nano-liposome and preparation method thereof

An epidermal growth factor and nano-liposome technology, which can be used in liposome delivery, skin care preparations, skin diseases, etc., can solve the problems of difficult to penetrate cell membranes, large particle size of ordinary liposomes, and has broad application prospects. , The product has good compatibility, and the effect of improving the stability of placement

Active Publication Date: 2019-02-12
NANJING SHINEKING BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, due to the large particle size of ordinary liposomes, it is difficult to penetrate the cell membrane, so it is inevitably subject to many restrictions in application.

Method used

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  • Polyglutamic acid and human epidermal growth factor nano-liposome and preparation method thereof
  • Polyglutamic acid and human epidermal growth factor nano-liposome and preparation method thereof
  • Polyglutamic acid and human epidermal growth factor nano-liposome and preparation method thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0077] Embodiment 1, encapsulation efficiency comparison of different preparation methods

[0078] The present invention adopts five liposome preparation methods suitable for water-soluble products: film dispersion method, reverse phase evaporation method, double emulsion method, ethanol injection method and ultrasonic method. Film dispersion-high pressure method, reverse phase evaporation-high pressure method, double emulsion-high pressure method, ethanol injection-high pressure method to prepare polyglutamic acid and human epidermal growth factor nano liposomes.

[0079] The concrete formula that five kinds of preparation methods of present embodiment are used is as follows:

[0080] Based on 100mL nano-liposome, its composition is:

[0081] Polyglutamic acid with a molecular weight of 800,000 Da: 10 mg

[0082] Human epidermal growth factor: 0.5mg

[0083] Soy lecithin: 3g

[0084] Cholesterol: 0.5g

[0085] ε-polylysine: 0.5mg

[0086] Propylene glycol: 0.1g

[0087...

Embodiment 2

[0097] Embodiment 2, the preparation of nano liposome

[0098] Based on 100mL nano-liposome, its composition is:

[0099] Polyglutamic acid with a molecular weight of 10000Da: 1mg

[0100] Polyglutamic acid with a molecular weight of 1,000,000 Da: 3 mg

[0101] Human epidermal growth factor: 0.2mg

[0102] Soy lecithin: 1.2g

[0103] Cholesterol: 0.3g

[0104] ε-polylysine: 0.1mg

[0105] Propylene glycol: 0.02g

[0106] NaCl: 0.02mg

[0107] Phosphoric acid: appropriate amount

[0108] The balance is water.

[0109] Its preparation method is film dispersion-high pressure method, specifically as follows:

[0110] Step 1: Dissolve polyglutamic acid, human epidermal growth factor, ε-polylysine and NaCl in a certain amount of water to form an aqueous phase, and adjust the pH to 6.5; mix soybean lecithin, cholesterol, propylene glycol with 38g of absolute ethanol After mixing, an oily phase is formed;

[0111] Step 2: Put the oil phase in a round bottom flask and evapor...

Embodiment 3

[0115] Embodiment 3, the preparation of nano liposome

[0116] Based on 100mL nano-liposome, its composition is:

[0117] Sodium polyglutamate with a molecular weight of 8000Da: 2mg

[0118] Sodium polyglutamate with a molecular weight of 600,000 Da: 2 mg

[0119] Human epidermal growth factor: 0.5mg

[0120] Soy lecithin: 1.5g

[0121] Cholesterol: 0.3g

[0122] ε-polylysine: 0.8mg

[0123] Propylene glycol: 0.08g

[0124] NaCl: 0.05mg

[0125] Sodium hydroxide: appropriate amount

[0126] The balance is water.

[0127] Its preparation method is film dispersion-high pressure method, specifically as follows:

[0128] Step 1: Sodium polyglutamate, human epidermal growth factor, ε-polylysine and NaCl are dissolved in a certain amount of water to form an aqueous phase, and the pH is adjusted to 9; after mixing soybean lecithin, cholesterol, propylene glycol and 18g chloroform form an oily phase;

[0129] Step 2: Put the oil phase in a round bottom flask and evaporate u...

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Abstract

The invention belongs to the technical field of bioengineering and discloses a polyglutamic acid and human epidermal growth factor nano-liposome and a preparation method thereof. The nano-liposome comprises high molecular weight polyglutamic acid, low molecular weight polyglutamic acid, a human epidermal growth factor and a liposome membrane material. According to the polyglutamic acid and human epidermal growth factor nano-liposome, the high molecular weight polyglutamic acid and the low molecular weight polyglutamic acid are compounded with the human epidermal growth factor and the preservative-free nano-liposome is prepared by utilizing a bacterium-inhibition effect of epsilon-polylysine; the nano-liposome has an addition and multiplying effect on skin repairing; the transdermal performance and skin absorption rate of active components are greatly improved; the nano-liposome has stable performance, good utilization compatibility and relatively high encapsulation rate.

Description

technical field [0001] The invention belongs to the technical field of biological materials, and in particular relates to a polyglutamic acid and human epidermal growth factor nano liposome and a preparation method thereof. Background technique [0002] As an emerging drug carrier, liposomes have been widely used in pharmaceutical preparations. However, due to the large particle size of ordinary liposomes, it is difficult to penetrate the cell membrane, so it is inevitably subject to many restrictions in application. Therefore, people began to think about designing a new type of liposome that can break through this limitation, so nano-liposomes were born. The nanometer size endows liposomes with good penetrability and special targeting, and many people are focusing on this new type of liposomes, and are committed to introducing it into the field of medicine and cosmetics, so that it can give full play to the treatment of diseases and beauty benefits. People name this new ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/127A61K47/24A61K38/18A61K8/14A61K8/64A61K8/88A61P17/02A61Q19/00A61K31/785
CPCA61K8/14A61K8/64A61K8/88A61K9/127A61K31/785A61K38/1808A61K47/24A61Q19/00A61K2300/00
Inventor 徐虹詹伊婧冯小海任战坤胡左殷
Owner NANJING SHINEKING BIOTECH CO LTD
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