Drug-carrying liposome co-modified by folic acid and TAT peptide and preparation method thereof

A liposome and co-modification technology, which is applied in the field of drug-loaded liposomes co-modified with folic acid and TAT peptide and its preparation field, can solve the problems of limited application and lack of cell selectivity of cell-penetrating peptides, and improve the transport efficiency. , the density is easy to control, the effect of reducing steric hindrance
CN103976954AInactive Publication Date: 2014-08-13SUZHOU UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
SUZHOU UNIV
Publication Date
2014-08-13
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses drug-carrying liposome co-modified by folic acid and TAT peptide and a preparation method thereof. The drug-carrying liposome comprises liposome, a long chain targeted membrane material, a short chain targeted membrane material and a drug. Meanwhile, the invention provides the preparation method of the drug-carrying liposome co-modified by folic acid and TAT peptide. The method comprises the following steps: weighing phospholipid, cholesterol, the long chain targeted membrane material, the short chain targeted membrane material and the drug, dissolving the components in an organic solvent, and then carrying out rotary evaporation at 50 DEG C under reduced pressure to remove the organic solvent so as to obtain a medicated liposome membrane; adding a phosphate buffer solution into the medicated liposome membrane for dissolving, carrying out ultrasonic treatment for 2 minutes, and filtering for 10 times by using a 0.22mu m membrane to obtain double-target drug-carrying liposome. According to the drug-carrying liposome disclosed by the invention, the TAT peptide is connected with specific ligands by means of PEG with different weight-average molecular weights to establish a nanometer carrier modified by double ligands, and the prepared drug-carrying liposome can be used for efficiently conveying the drug in tumor cells.
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Description

technical field

[0001] The invention belongs to the field of drug carriers, in particular to a drug-loaded liposome co-modified with folic acid and TAT peptide and a preparation method thereof. Background technique

[0002] Nanocarriers have shown great potential in the tumor-selective delivery of chemotherapeutic drugs. It is worth noting that the antitumor effect of this nanotechnology-based treatment is still very limited, most likely because nanocarriers are not efficient enough to deliver chemotherapeutics. Drug delivery into tumor cells limits the antitumor activity of chemotherapeutic drugs. Nanocarriers enter the tumor interstitium through blood vessels mainly through convection and diffusion. At the same time, the high interstitial pressure (IFP) in the tumor stroma promotes the reverse flow of interstitial fluid into the surrounding tissue, thereby hindering the penetration of nanocarriers into the interior of solid tumors. If the nanocarriers that pass through t...

Claims

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