Method for preparing natural material-liposome composite nanofiber based on electrostatic spinning technology

A technology of composite nanofiber and electrospinning technology, which is applied in spinning solution preparation, fiber treatment, rayon manufacturing, etc., can solve the problems of poor stability of bioactive molecules, adverse reactions of patients, short half-life, etc., and achieve low price, Good application prospect, simple operation effect

Inactive Publication Date: 2012-11-28
DONGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Bioactive molecules have poor stability and are easily degraded by some enzymes in the body or self-degraded when encountering acid and alkali, and the short half-life requires frequent administration, which is likely to cause adverse reactions or even toxic reactions to patients

Method used

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  • Method for preparing natural material-liposome composite nanofiber based on electrostatic spinning technology
  • Method for preparing natural material-liposome composite nanofiber based on electrostatic spinning technology
  • Method for preparing natural material-liposome composite nanofiber based on electrostatic spinning technology

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Liposome-loaded gelatin nanofibrous scaffold

[0034] (1) Prepare liposome microspheres by film dispersion method. Weigh 0.23g of lecithin, 0.15g of cholesterol, and 0.02g of octadecylamine into a 50mL small beaker, add 1.5mL of absolute ethanol, place in a 65°C water bath, stir to dissolve, and evaporate the ethanol under reduced pressure. Liposome membranes were obtained.

[0035] (2) Take 30mL of deionized water in a small beaker, place it in a 65°C water bath, and keep it warm for later use.

[0036] (3) Add the product of step (2) to the small beaker in step (1), stir and hydrate in a water bath at 65°C for 10 minutes. Then place the small beaker on a magnetic stirrer and stir at room temperature for 30 minutes. If the volume of the solution decreases, add deionized water and mix well.

[0037] (4) Ultrasonicating the product of step (3) to obtain a liposome suspension with uniform particle size.

[0038] (5) Use the product in step (4) as a solvent to configur...

Embodiment 2

[0041] Liposome-loaded silk fibroin nanofiber scaffold

[0042] (1) Prepare liposome microspheres by film dispersion method. Weigh 0.3g of lecithin, 0.15g of cholesterol, and 0.02g of octadecylamine into a 50mL small beaker, add 1.5mL of absolute ethanol, place in a 65°C water bath, stir to dissolve, and evaporate the ethanol under reduced pressure. Liposome membranes were obtained.

[0043] (2) Take 30ml of deionized water in a small beaker and place it in a 65°C water bath to keep warm for later use.

[0044] (3) Add the product of step (2) to the small beaker in step (1), stir and hydrate in a water bath at 65°C for 10 minutes. Then place the small beaker on a magnetic stirrer at room temperature and stir for 30 minutes. If the volume of the solution decreases, add deionized water and mix well.

[0045] (4) Ultrasonicating the product of step (3) to obtain a liposome suspension with uniform particle size.

[0046] (5) The product in step (4) was used as a solvent to pre...

Embodiment 3

[0049] Liposome-loaded silk fibroin nanofiber scaffold

[0050] (1) Prepare liposome microspheres by film dispersion method. Weigh 0.3g of lecithin, 0.15g of cholesterol, and 0.02g of octadecylamine into a 50mL small beaker, add 1.5mL of absolute ethanol, place in a 65°C water bath, stir to dissolve, and evaporate the ethanol under reduced pressure. Liposome membranes were obtained.

[0051] (2) Take 50ml of deionized water in a small beaker, add 100μg of plasmid DNA, and place in a 65°C water bath to keep warm until use.

[0052] (3) Add the product of step (2) to the small beaker in step (1), stir and hydrate in a water bath at 65°C for 10 minutes. Then place the small beaker on a magnetic stirrer at room temperature and stir for 60 minutes. If the volume of the solution decreases, add deionized water and mix well.

[0053] (4) Ultrasonicating the product of step (3) to obtain a liposome suspension with uniform particle size.

[0054] (5) The product in step (4) was used...

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Abstract

The invention relates to a method for preparing a natural material-liposome composite nanofiber based on electrostatic spinning technology. The method comprises the following steps of: (1) adding lecithin, cholesterol and octadecylamine into a reaction vessel, then adding anhydrous alcohol, stirring for dissolving, and finally depressurizing to remove alcohol so as to obtain a liposome membrane; (2) adding deionized water into the reaction vessel containing the liposome membrane, stirring at room temperature, and then carrying out ultrasonic treatment so as to obtain a liposome suspension with uniform particle sizes; (3) preparing a spinning solution containing natural materials by taking the liposome suspension as a solvent, and then carrying out electrostatic spinning so as to obtain a nanofiber; and (4) fumigating and cross-linking the nanofiber in a genipin solution or alcohol steam. The method provided by the invention has the advantages of simplicity in operation, low cost of raw materials, mild reaction conditions and good biocompatibility; and a composite nanofiber bracket prepared by the method provided by the invention can control the release of genes, growth factors and various medicaments, has stable performance, is easy to preserve, and has wide application prospects.

Description

technical field [0001] The invention belongs to the field of preparation of liposome composite nanofibers, in particular to a method for preparing natural material-liposome composite nanofibers based on electrospinning technology. Background technique [0002] There are many ways to prepare nanofibers, such as: template synthesis, self-assembly, phase separation and electrospinning techniques. Compared with other techniques, electrospinning has its significant advantages. The template synthesis method refers to the use of a mold with a nanostructure to form a material into a fiber or catheter structure. The biggest advantage of this method is that many materials such as metals, electronically conductive polymers, semiconductor materials, and carbon materials can be prepared into nanoscale by using templates. fibers or conduits. However, this method cannot realize the preparation of one-to-one continuous nanofibers. The phase separation method mainly uses different solvent...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): D01F9/00D01F4/00D01F8/02D01F8/18D01F1/10D01D5/00D01D1/02A61L27/20A61L27/22A61L27/24A61L27/54
Inventor 王红声崔呈俊何创龙莫秀梅
Owner DONGHUA UNIV
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