141 results about "Template synthesis" patented technology

The synthesized image generation method for generating synthesized image data by extracting face images of characters contained in image data and synthesizing the extracted face images with a template into a plurality of synthesis positions which have been set in advance on the template, the method comprising the steps of: forming groups of the synthesis positions by combining the adjacent synthesis positions; determining an order of the groups according to number of synthesis positions belonging to each of the groups; detecting the face images of the characters contained in the image data; detecting a relationship between the detected face images; distributing the face images to the groups in the determined order according to the relationship between the face images; and generating the synthesized image data by synthesizing the distributed face images with the template into the synthesis positions of the groups.

Systems and methods are described for synthesis of films, coatings or layers using templates. A method includes locating a template within at least one of a first precursor layer that is coupled to a first substrate and a second precursor layer that is coupled to a second substrate; forming a composition layer; and moving the first substrate relative to the second substrate, wherein the composition layer remains coupled to the second substrate.

The extensive synthesis ("amplification") of a nucleic acid sequence of interest is attained through a linked series of multi-cycle primer extension reactions (LLA). The primers used in each of the primer extension reactions of the process contain non-replicable and / or cleavable elements that halt nucleic acid synthesis and thereby prevent the synthesized molecules from serving as templates in subsequent cycles. Synthesized molecules accumulate during primer extension in a mathematically linear fashion, thereby rendering the process relatively insensitive to contaminating nucleic acids. Multiple primer sets are employed, and simultaneously included in the reaction mixture, thereby ensuring the accumulation of a large number of copies of the nucleic acid sequence of interest. The invention also provides for the detection of an amplified nucleic acid sequence of interest, as well as reagent kits for carrying out the reaction.

The present invention relates to a method and a device for verifying identity of an individual by employing biometric data derived from a physical feature of the individual. A basic idea of the invention is that, rather than determining peak locations in cyclic signals such as ECG or PPG signals when using these signals as a representation of biometric data for verifying identity of an individual, shape or morphology of the signals is considered.

The present invention relates to methods for fabricating a laterally-limited two-dimensional structure through template synthesis. The methods of the invention are useful in forming homogenous and heterogeneous layered materials. The invention also provides structures and devices formed by the method of the present invention and uses thereof.

The invention discloses a method for synthesis of a double-casing layer carbon nanometer hollow polyhedron by a metal-organic framework as a template and belongs to the field of novel energy and novel materials. The method comprises that a zeolitic imidazolate framework with a core-shell nanometer structure as a structure precursor is calcined at a high temperature to form the double-casing layer nanometer carbon hollow polyhedron. The cheap and easily available zeolitic imidazolate framework as a structure precursor is used for preparation of the double-casing layer carbon nanometer hollow polyhedron. The method has simple processes, is free of a template, realizes precise control of a shell structure by change of a zinc / cobalt-based zeolitic imidazolate framework ratio or calcining conditions, is environmentally friendly and can be industrialized easily. The double-casing layer carbon nanometer hollow polyhedron has a wide application prospect in the fields of energy storage, catalysis, photoelectric materials and drug transport.

A method of preparing a DNA copy of a target polynucleotide using template switching is described. The method includes mixing a double stranded template / primer substrate made up of a DNA primer oligonucleotide associated with a complementary oligonucleotide template strand with a target polynucleotide in a reaction medium and adding a suitable amount of a non-retroviral reverse transcriptase to the reaction medium to extend the DNA primer oligonucleotide from its 3′ end to provide a DNA copy polynucleotide. The DNA copy polynucleotide includes a complementary target DNA polynucleotide that is synthesized using the target polynucleotide as a template. Methods of adding nucleotides to the double stranded template / primer substrate are also described. The method can be used to facilitate detection, PCR amplification, cloning, and determination of RNA and DNA sequences.

The invention relates to a method for synthesizing a high-fluorescent alloy nano cluster by taking small molecules as a template, and belongs to the technical field of bioengineering. The method comprises the following steps: (1) mixing glutathione solution with copper ion solution uniformly, and regulating the pH to 4-5, wherein the mole rate of glutathione to copper ions is (4-8):1; and (2) adding silver ions into a product obtained in the step (1), and mixing uniformly, wherein the mole rate of glutathione to copper ions to silver ions is (20-40):5:(4-6). The method provided by the invention has the beneficial effects that by introducing the silver ions to synthesize an alloy copper-silver nano cluster, the fluorescence intensity of a copper nano cluster is enhanced, the stability of the cluster is improved, and a new nano cluster is provided. Moreover, the synthetic method provided by the invention is easy to control, and is simple and quick.

The present invention provides methods and compositions for expanding the scope of chemical reactions that can be performed during nucleic acid-templated organic syntheses. In particular, nucleic acid-templated chemistries are used to produce reaction intermediates attached to an oligonucleotide that can be used to identify the reaction intermediates and / or the resulting reaction products. The reaction intermediates then are reacted with free reactants (for example, reactants that are difficult or impractical to couple to an oligonucleotide) to produce a reaction product. This approach expands the scope of reagents useful in nucleic acid-templated syntheses to reagents that do not need to be or cannot be tethered to an oligonucleotide. The reagents, however, still permit the synthesis of reaction products attached to oligonucleotides that can be used to identify the reaction products.

The invention discloses a preparation method of a SAPO-18-carried Cu-Fe catalyst for ammonia-selective catalytic elimination of NOx. The SAPO-18-carried Cu-Fe catalyst is used for selective catalytic elimination of nitric oxides. The preparation method is characterized in that SAPO-18 foraminate molecular sieve carriers having high specific surface areas of 400 to 550m<2> / g are prepared by a soft template synthesis method; and a FeCl3-CuCl2 mixed solution and the SAPO-18 foraminate molecular sieve carriers are prepared into the SAPO-18-carried Cu-Fe catalyst by an ion exchange method. In a wide temperature range of 150 to 550 DEG C, the SAPO-18-carried Cu-Fe catalyst has high catalytic elimination effects on pollutants having a high airspeed of 120000 to 360000mL.(g.h)<-1>, a high O2 concentration of 10 to 20vl.%, high H2O content of 5 to 10wt% and a low NO concentration of 300 to 1000ppm, and has high stability. After adding a hydrocarbon (300 to 1000ppm of propylene) into a reaction system, the SAPO-18-carried Cu-Fe catalyst has high carbon deposition resistance and high hydrothermal stability.

The present invention provides novel compositions and methods for suppressing the expression of a targeted gene using RNA-DNA hybrid constructs. The invention further provides novel methods and compositions for generating or producing RNA-DNA hybrids, whose quantity is high enough to be used for the invention's gene silencing transfection and possibly in therapeutics applications. This improved RNA-polymerase chain reaction method utilizes thermocycling steps of promoter-linked DNA or RNA template synthesis, in vitro transcription and then reverse transcription to bring up the amount of RNA-DNA hybrids up to two thousand folds within one round of the above procedure for specific gene silencing.

The invention discloses a method for preparing a zinc-doped iron trioxide composite structure gas-sensitive element and application thereof and belongs to the field of preparation of nanometer functional materials. The gas-sensitive element is a nanocomposite material synthesized by taking MOFs as a template. The preparation method specifically comprises the following steps: preparing a Zn-doped MIL-88B nano spindle by adopting a one-step solvothermal method; performing centrifugal drying on the obtained product, calcining in an air atmosphere, and finally obtaining Zn-doped alpha-Fe2O3 nanocomposite material powder; further adding terpilenol into the obtained nano powder, uniformly grinding, smearing on a ceramic tube, sintering in a muffle furnace, thereby obtaining the gas-sensitive element. The gas-sensitive element has the gas-sensitive characteristics of low concentration detection, fast response, high stability and high selectivity and can be used for preparing semiconductor gas-sensitive sensors.

A transition-metal-AEI / ITE molecular sieve catalyst and mixed-template synthesis procedure are disclosed.

The invention involves preparation method of three-dimensional network hierarchy photocatalysis materials which crystal phase can be controlled. The method bases on biological template synthesis technology, uses bird eggshell membrane as biological template material, dips the eggshell membrane into titanates prodromal liquid to handling the dip optimization after separating, washing and pretreating the eggshell membrane, then oxygenizes and incandesces it to forming titania nanometer crystal under the action of biological template in original position, then prepares nanometer titania with excellent performance and three-dimensional network hierarchy as photocatalysis material. The prepared photocatalysis material consists of mixing-phase titania crystal grain which grain size is below 20nm, the component percentage of anatase phase and rutile phase can be controlled by adjusting the pH of steeping liquor. The process of the invention is simple, cost is low and the performance of prepared photocatalysis material is stable; it can be used for gas sensor, pressure-active element, nonlinear optical material, solar battery and environmental purification material, it also has good prospect in the electrical, electronic, industrial and civil fields.

A method for template-directed sequencing-by-synthesis of an array of target polynucleotide can include:(a) providing an array of target polynucleotides in a fluidic vessel;(b) contacting the array of polynucleotides with a solution comprising (i) polymerization complex and (ii) reversibly terminating and differently labeled A,C,G, and T / U nucleotides;(c) incorporating one of the differently labeled nucleotides, using the polymerization complex, into a chain complementary to at least one of the array of polynucleotides;(d) binding imaging tags to the differently labeled nucleotides of step (c);(e) imaging and storing the identity and position of the imaging tags of step (d);(f) reversing termination (b)-(e);(g) repeating steps (b)-(e) and assembling a sequence for each of the array of target polynucleotides from the stored identity and position of the imaging tags, optionally as a homogeneous or one pot reaction. Additional methods of sequencing target polynucleotides are described herein.

A ZSM-5 molecular sieve, a preparing method thereof and applications of the molecular sieve are provided. The ZSM-5 molecular sieve is prepared through an amine-free system. A silica-alumina ratio of the ZSM-5 molecular sieve is 40-200. The ZSM-5 molecular sieve is prepared by adopting a nanometer thin-layer ZSM-5 molecular sieve having a high silica-alumina ratio as a crystal seed with the mole ratio of raw materials meeting a condition that the mole ratio of SiO2 to Al2O3 is 100:(0.04-2). Compared with molecular sieves synthesized under traditional template-free conditions, the ZSM-5 molecular sieve synthesized by the method is a molecular sieve having a high silica-alumina ratio (higher than 40). The silica-alumina ratio of the prepared flake ZSM-5 molecular sieve is adjustable in a large range (40-200).

The invention provides a method for producing polymers having a desirable property, for example, catalytic activity or binding activity, via evolutionary nucleic acid-mediated chemistry.

The invention discloses an organic additive assisted hydrothermal synthesis method for one-dimensional nanometre morphology manganese lithium silicate anode material, belonging to the technical field of electrochemical electrical source materials. In the method, cheap organic additives including polyvinyl alcohol (PVA) and ascorbic acid (VC) are adopted as morphology guide modulators, so that an one-dimensional nanometre morphology manganese lithium silicate anode material is directly acquired by combining a simple and easy soft template synthesis method with the hydrothermal synthesis method; simultaneously, a carbon source is in-situ introduced, and a calcining technology is further adopted to in-situ generate an Li2MnSiO4 / C composite anode material, so that the electrochemical performance of the electrode is greatly improved. Relative to a solid-phase method and a sol-gel method, a pure phase can be acquired easily, and battery charging and discharging performance of the electrode is greatly improved. Besides, the synthesis method provides a method for preparing manganese lithium silicate one-dimensional nanometre anode material and has a wide application prospect in the lithium-ion battery anode material.

The invention discloses a method for synthesizing cationic polyacrylamide by a method using ultrasound to induce template polymerization. The method is as follows: adding an acrylamide monomer, a cationic monomer, deionized water, a template and urea in turn in a reaction device, stirring uniformly, adjusting pH, processing the system into a homogeneous solution under ultrasonic irradiation, introducing nitrogen gas for deoxygenation, immediately sealing the reaction device after adding an initiator, finally, performing ultrasonic treatment at a certain frequency to induce polymerization, purifying, washing, refining, drying in a vacuum drying box, and grinding to obtain a final product. The cationic monomer of the cationic polyacrylamide prepared by the method is concentrated in distribution, large in molecular weight and high in charge density, and greatly improves the electric neutralization and bridging capacity of a flocculant. The method has the advantages of short polymerizationtime, low reaction temperature, high polymerization efficiency and simple experimental operation, reduces energy consumption and production cost, and suitable for dehydration treatment of drinking water, sewage and sludge, and has good application prospects.

The invention provides a preparation method of mesoporous zeolite by utilizing a polyelectrolyte-surfactant composite as a template, and belongs to the technical field of organic chemistry, physical chemistry, and catalytic chemistry. The preparation method comprises following steps: utilizing conventional silicon source and aluminum source, taking a low cost anion polyelectrolyte-surfactant composite as a template, adopting an one-pot method, hydrothermally crystallizing at the temperature of 140 DEG C, and burning at the temperature of 550 DEG C for 6 hours so as to obtain mesoporous silicalite-1 and mesoporous ZSM-5. The preparation method utilizes the polyelectrolyte (polyacrylic acid PAA)-surfactant (cetyl trimethyl ammonium bromide CTAB) composite as the template to prepare mesoporous zeolite with hole sizes of 5 to 20 nm, mesoporous ZSM-5 has a high catalytic activity in catalytic reactions; the adding sequence of NaCl is changed so as to synthesize mesoporous silicalite-1 with a hole size of about 3 nm, furthermore, the mesopores are smaller and the array of mesopores is tighter; mesoporous silicalite-1 can also be prepared from another polyelectrolyte (polyacrylic acid PAA)-surfactant (cetyl pyridinium chloride) composite, indicating that the method of synthesizing mesoporous zeolite by utilizing a polyelectrolyte-surfactant composite as the template can be widely popularized, and has a potential application value in the field of catalysis.

The invention relates to a dynamic page generation method and system with loose relation to business logic and belongs to the page generation technical field. The dynamic page generation system comprises the page management side and the page release side; the page management side is mainly used for managing release resources, a release structure and template definition, wherein the management comprises defining the release resources, a resource template, a release rule, a directory structure, a website domain name and a release page, enabling a produced template to be introduced into the generation system through a template management program and sending a task message to the release side automatically; the page release side is mainly used for performing generation processing through monitoring of a message file sent from the page management side and enabling the template to be converted into a dynamic page program of a website; the page release side is used for performing regular scanning, multitasking thread processing, template synthesis, calculation of a page outside network path and dynamic page conversion specifically and namely that the custom markup language in the template is replaced by a section of Java codes and a dynamic result page can be eventually generated.

The invention belongs to the technical field of a synthesis method of ZSM-5 molecular sieves, and particularly relates to a method for synthesizing ordered mesoporous ZSM-5 through in-situ template carbonization. The method comprises steps as follows: step one, an SBA-15 / P123 compound and an MCF-P123 compound are prepared; step two, the SBA-15 / P123 compound and the MCF-P123 compound are carbonized respectively; step three, the ordered mesoporous ZSM-5 is synthesized with a steam assisted crystallization method. According to the synthesis method, SBA-15 with a P123 template which is not removed is subjected to in-situ two-step carbonization, namely, sulfuric acid pre-carbonization and argon high-temperature carbonization at 900 DEG C, so that the internal P123 soft template is carbonized into an ordered mesoporous hard template to the greatest extent, after impregnation with a TPAOH solution and steam assisted crystallization, air calcination is performed to remove carbon finally, and two crystalized ordered mesoporous ZSM-5 molecular sieves with different mesopore sizes are obtained.

The present invention relates to a bioreactor for RNA in vitro transcription, a method for RNA in vitro transcription, a module for transcribing DNA into RNA and an automated apparatus for RNA manufacturing. Further, the use of a bioreactor for RNA in vitro transcription as described herein is part of the present invention. The present invention relates to an RNA in vitro transcription reactor designed to be operable in an automated manner under GMP-compliant conditions. In particular, said RNA in vitro transcription reactor allows repetitive use of DNA template for various RNA in vitro transcription reactions. Further, the invention relates to an apparatus for RNA manufacturing comprising (a) a module for template DNA synthesis, (b) a module for transcribing DNA into RNA comprising said RNA in vitro transcription reactor, and, optionally, (c) a module for RNA formulation.

The invention relates to a nano-nickel rod array atomic force microscope tip characterization sample and a manufacturing method thereof. The method comprises the following steps of: using a secondary anodization method to obtain a porous alumina template by processing; by using an electron-beam evaporation based template synthesis method, evaporating nickel ions on the porous alumina template to form a nickel film; and fixedly supporting the nickel film with a nanorod array structure, removing the porous alumina template on the nickel film, and finally forming the nickel film with the nanorod array structure ( 1 ), i.e., the nano-nickel rod array atomic force microscope tip characterization sample. The method has a simple process and a low manufacturing cost, and the size, aspect ratio and density of the processed nano-nickel rod array structure can be realized by adjusting the structure of the porous alumina template, so that the nano-nickel rod array structure has larger degree of freedom. The nano-nickel rod array atomic force microscope tip characterization sample with small characteristic size, high aspect ratio and good consistency can be manufactured by the method.

A preparation method of hollow metal microspheres relates to a composite material. The invention provides a preparation method of hollow metal microspheres which is quick, environment-friendly and simple in subsequent treatment process. The preparation method comprises the steps of preparing gold nanoparticles and titanium dioxide successively, and then functionalizing the surface of titanium dioxide, and then preparing Au / TiO2, and finally preparing the hollow metal microspheres. Titanium dioxide microspheres are used as templates, and dimension-controllable PtM metal hollow microspheres are prepared by means of the pH sensitivity of the titanium dioxide. The size of the PtM metal hollow microspheres can be controlled by regulating the size of the template titanium dioxide, and simultaneously, the thickness of the PtM metal hollow microspheres can be changed by changing the concentration of the metal precursor. Multi-layer PtM hollow microspheres are synthesized with the titanium dioxide as the templates; and therefore, the preparation method of the hollow microspheres is expanded. Besides, the technical conditions are mild and easy to control, the reaction time is short, the templates are easy to remove without use of a high temperature or a strong acid and a strong base, and the like, and simultaneously, the subsequent treatment process is simple.

A transition-metal-CHA molecular sieve catalyst and mixed-template synthesis procedure are disclosed.

The invention provides a method for preparing porous carbon from ethylene tar, and belongs to the field of advanced processing of petrochemical byproducts. The method comprises the following steps: preprocessing and pre-oxidizing ethylene tar, and uniformly dispersing the pre-oxidized ethylene tar in an alkali solution with a certain concentration; dropwise adding a magnesium precursor to the above obtained colloid, standing the obtained mixture overnight, carrying out centrifuge and drying to obtain a carbon precursor and template agent coprecipitate, and carrying out high-temperature charring and pickling in inert atmosphere to obtain the target product. The in-situ template synthesis method provided by the invention realizes homogeneous mixing of the precursor and the template and regeneration of the pickled template and also realizes the material circulation of the whole technology, and the prepared porous carbon has narrow aperture distribution, has a specific surface area reaching 1620 m<2> / g, has a specific capacitance reaching 260 F / g, can be used as an energy storage material and an electrode material, and realizes high-added value utilization of the ethylene tar.

The invention discloses a technology for enhancing fluorescence and improving anti-oxidant activity of DNA-Ag NCs synthesized by the DNA template method. The fluorescence intensity of the DNA-Ag NCs synthesized by taking a cytosine-rich oligonucleotide as a template is remarkably enhanced in the presence of Ag nano-particles with stable sodium citrate, and the anti-oxidant activity of the DNA-Ag NCs in saturated dissolved oxygen is also improved obviously. The technology provided by the invention has the advantages of simple operation, high speed and efficiency, low cost and the like.

A process for preparing the hollow copper sulfide nano-balls with optical clipping performance features that the binding of surfactant micelles is used, the resultant ammonia gas is used to form nucleating center, and the simple soft template synthesis method is used. It can be used in military, laser and microelectronic fields.

The invention discloses a rare earth phthalocyanine sandwich layered structured compound and a preparation method thereof. According to the method, a "solid phase" or a "liquid phase" template synthesis method is adopted to synthesize the compound in one step, and then through a purification technology with a combination of specifically designed recrystallization, washing and thermal vacuum treatment, the high-purity rare earth phthalocyanine sandwich layered structured compound is obtained, wherein the layer spacing of the compound is 2-4angstroms, and effective reversible intercalation and deintercalation of lithium ions can be realized. The synthesis technology disclosed in the invention has high yield, less side products, high target compound purity, simple operation process and low cost and is suitable for industrial large-scale production; the capacity of the negative electrode material is obviously higher than the theoretical capacity (372 mAh / g) of the conventional graphite negative electrode material; in addition, the negative electrode material has very high initial coulombic efficiency and cycle performance; and therefore, the lithium ion battery assembled by taking therare earth phthalocyanine sandwich layered structured compound as the negative electrode material has excellent electrochemical performance and practical value.