22 results about "Template synthesis" patented technology

The invention discloses a preparation method of a space chip, the space chip and application of the space chip, the preparation method comprises the following steps: hybridizing: connecting a single-stranded nucleic acid molecule to the surface of a solid-phase substrate, the single-stranded nucleic acid molecule comprises a first fixed sequence, a space bar code sequence, a sequencing primer binding sequence and a second fixed sequence, the surface of the solid-phase substrate of the chip substrate is connected with a first probe, and the first probe comprises a first fixed sequence complementary sequence and a second fixed sequence complementary sequence; carrying out ring closing to enable the single-stranded nucleic acid molecules to form annular nucleic acid molecules; carrying out rolling circle amplification; sequencing to obtain sequence information of the space bar code sequence; synthesizing single-stranded nucleic acid molecules by taking the rolling circle amplification product as a template to obtain a single-stranded nucleic acid molecule library; fixing and clustering: fixing the single-stranded nucleic acid molecule library on the surface of a solid-phase substrate to form a single-stranded nucleic acid molecule cluster. According to the invention, the chip with the nucleic acid probe carrying spatial position coordinate information can be prepared.

The invention belongs to the technical field of new energy, and particularly relates to a manganese dioxide composite material for a battery and a preparation process thereof. Comprising the following steps: preparing iron ion loaded cattail wool; synthesizing beta-manganese dioxide by a hydrothermal template method; and carrying out vacuum impregnation and calcination treatment on the precursor template. Fe < 3 + > ions are loaded on the surface of cattail wool, glucose is doped while beta-manganese dioxide is synthesized, oxygen atoms can be induced to be separated from crystal lattices so as to form oxygen vacancies during subsequent calcination, the surface of manganese dioxide is coated with residual carbon generated by pyrolysis, backfilling of the oxygen vacancies can be inhibited, and the oxygen vacancies can be effectively degraded. The oxygen vacancies formed after calcination can increase the storage sites of carriers (Zn < 2 + > and H < + >), and endow the subsequent manganese dioxide composite material with higher specific capacity, thereby effectively increasing the discharge specific capacity of the manganese dioxide composite material as the positive electrode of the battery.

The present invention provides organic-inorganic hybrid mesoporous silica nanoparticles, their self-template synthesis method and application. Long-chain alkylamino groups are connected to the surface of silica nanoparticles, wherein the long-chain alkylamino groups are dialkyl-substituted alkylamino groups, and the length of the alkyl substituent on the nitrogen atom in the amino group is 1-3, thereby forming a mesoporous structure. The present invention uses the long-chain alkylamino groups as self-templates and synthesizes the mesoporous silica nanoparticle material by a reverse microemulsion method. The organic-inorganic hybrid mesoporous silica nanoparticle material can be used as an adsorbent to remove various organic pollutants in water and has a large adsorption capacity and high adsorption efficiency.

The present invention relates to a bioreactor for RNA in vitro transcription, a method for RNA in vitro transcription, a module for transcribing DNA into RNA and an automated apparatus for RNA manufacturing. Further, the use of a bioreactor for RNA in vitro transcription as described herein is part of the present invention. The present invention relates to an RNA in vitro transcription reactor designed to be operable in an automated manner under GMP-compliant conditions. In particular, said RNA in vitro transcription reactor allows repetitive use of DNA template for various RNA in vitro transcription reactions. Further, the invention relates to an apparatus for RNA manufacturing comprising (a) a module for template DNA synthesis, (b) a module for transcribing DNA into RNA comprising said RNA in vitro transcription reactor, and, optionally, (c) a module for RNA formulation.

A DNA synthesis method is provided. The DNA synthesis method according to some embodiments is a method for synthesizing a target DNA to be used as a data storage medium, and may comprise the steps of: preparing a template DNA; and synthesizing the target DNA from the template DNA on the basis of a DNA polymerase. Here, the sequence of the template DNA includes a universal base sequence, which can complementarily bind to any one of two or more types of data DNA bases, and a stop base sequence, wherein a starting base of the stop base sequence does not complementarily bind to the data DNA bases and can complementarily bind to a pre-designated non-data DNA base. According to this method, a target DNA having a desired sequence can be accurately and quickly synthesized in an eco-friendly manner.

The invention provides a cell-free RNA (Ribonucleic Acid) synthesis method which is named as a single-stranded template in-vitro transcription method, wherein single-stranded linear DNA is prepared by amplifying double-stranded annular DNA and is used as an IVT template. Wherein the double-stranded circular DNA comprises a nicking enzyme binding and cleavage site and an RNA polymerase binding site, and the method comprises the following steps: a) contacting the double-stranded circular DNA with a nicking enzyme capable of recognizing the nicking enzyme binding and cleavage site to obtain a mixture; b) contacting the mixture in the step a) with constant-temperature DNA polymerase for rolling circle amplification to obtain single-chain linear multi-conjoined DNA; c) annealing the oligonucleotide fragment with the RNA polymerase binding site to form a local double strand; and d) contacting the local double strand with an RNA polymerase to initiate transcription. According to the method disclosed by the invention, large-scale RNA production can be carried out only by a small amount of plasmid templates, and a complete GMP three-level strain library does not need to be established. The DNA template is synthesized by adopting an in-vitro enzyme method, and then the RNA is synthesized, so that the raw materials are clear, and the system is simple.

The invention relates to the technical field of photocatalysts, in particular to preparation of a porous nano-zinc oxide photocatalyst and application of the porous nano-zinc oxide photocatalyst in antibiotic degradation, which comprises the following steps: S1, selecting and preparing a template, selecting and preparing a proper template material which is an ordered porous material; s2, preparing a precursor solution; s3, filling a precursor; s4, material deposition; s5, removing the template; s6, post-treatment, wherein the obtained material is washed, dried and subjected to heat treatment; and S7, performance testing. The porous nano zinc oxide photocatalyst is prepared by a template synthesis method, a nano structure with a specific shape and size can be prepared, the porous structure provides a relatively high specific surface area and is beneficial to improvement of catalytic efficiency, a nano material with uniform size distribution can be prepared by the template synthesis method, and the method has good repeatability and is suitable for industrial production. The method is suitable for large-scale production.

The application discloses a kind of supermolecule self-decomposition template synthesis multi-element doped diameter adjustable carbon-based micropipe method, belong to carbon micropipe preparation technical field, operation steps are as follows: (1) respectively preparation containing melamine (M) or 2,4,6-triaminopyrimidine (TAP) solution A and containing cyanuric acid (CA) or trithiocyanuric acid (TCA) solution B;(2) A liquid drop is added to B liquid, and the supermolecule precursor of M-CA, M-TCA, TAP-TCA is obtained;(3) 2-methylimidazole and sulfur, boron, phosphorus, fluorine and other element compounds are added in the solution of precursor, and after heating and drying, the precursor composite with different diameters is obtained by drying in oven for 24h;(4) it is obtained by calcining at 500-700 DEG C for 1-2h under argon atmosphere;In the application, the synthesized supermolecule as a template has the advantages of self-decomposition, adjustable diameter, and can be partially converted into carbon-based materials;During preparation, adding appropriate drugs realizes multi-element doping in one step, and the method has the advantages of simplicity, low cost and large-scale preparation.

The present invention relates to the use of templates with junction regions in the circularisation of linear nucleic acids; and to templates with junction regions for use in nucleic acid synthesis, methods of making said templates for use in nucleic acid synthesis, and methods of using said templates to synthesise nucleic acids.

The present invention relates to a bioreactor for RNA in vitro transcription, a method for RNA in vitro transcription, a module for transcribing DNA into RNA and an automated device for RNA production. In addition, the use of a bioreactor for RNA in vitro transcription as described herein is part of the invention. The present invention relates to an RNA in vitro transcription reactor designed to be operable in an automated manner under GMP compliance conditions. In particular, the RNA in vitro transcription reactor allows a DNA template to be repeatedly used for various RNA in vitro transcription reactions. Furthermore, the present invention relates to a device for RNA production comprising: (a) a module for template DNA synthesis, (b) a module for transcribing DNA to RNA, the module comprising the RNA in vitro transcription reactor; and optionally (c) a module for an RNA preparation.

The invention discloses a preparation method of a silver superstructure material, which adopts a one-pot method and template-free synthesis, and specifically comprises the following steps: S1, dissolving soluble silver salt in a certain solvent to obtain a silver salt solution with a certain concentration; s2, alkali is added into the silver salt solution obtained in the step S1, and the pH value of the silver salt solution is adjusted through the alkali; and S3, adding a reducing agent into the silver salt solution obtained in the step S2, and reacting for a period of time at a certain temperature to obtain the silver superstructure material. According to the preparation method of the silver superstructure material, the silver superstructure material is synthesized by adopting a one-pot method without a template, the preparation process is simple, the operation is convenient, the preparation cost is low, and large-scale industrial production is facilitated. Meanwhile, the silver superstructure material has the characteristic of large grain boundary density, can be used for high-selectivity electro-catalysis of reduction of carbon dioxide to form a carbon monoxide product, and has the advantages of good product selectivity, large current density, strong catalytic stability and the like.

The invention discloses a zinc-based modified molecular sieve adsorbent for removing hexafluoropropylene oxide in trifluoroacetyl fluoride and a preparation method of the zinc-based modified molecular sieve adsorbent, and belongs to the technical field of fluorine-containing fine chemical purification and multi-phase adsorption materials. Comprising the following steps: (1) soaking a molecular sieve carrier in an ammonium fluoride aqueous solution for treatment; (2) dispersing the product in the step (1) in absolute ethyl alcohol, and adding methyltrimethoxysilane for reaction to obtain a pretreated molecular sieve; (3) putting the pretreated molecular sieve into a zinc source aqueous solution, and stirring for reaction; (4) roasting the dried product in an air atmosphere; and (5) cyclically carrying out the steps (1)-(4) for 1-5 times to obtain a finished product. The adsorbent provided by the invention has the advantages of good thermal stability, excellent regeneration performance, template-free synthesis, low cost and the like, and is suitable for an industrial continuous adsorption process environment for purifying electronic-grade trifluoroacetyl fluoride.

This invention belongs to the field of molecular sieve preparation technology, specifically disclosing a non-transformable solid-phase template-free method for synthesizing molecular sieves. The invention first grinds and mixes a silicon source, an aluminum source, sodium hydroxide, potassium hydroxide dispersion, and seed crystals (CHA or PHI molecular sieve seed crystals) to obtain a mixture; then, the mixture is crystallized to obtain CHA or PHI molecular sieves. The preparation method disclosed in this invention, due to the absence of solvents, results in high product yield, low-pressure and safe synthesis process, simple process, no expensive molecular sieve raw materials, and no need for calcination to remove organic components. This eliminates organic pollution and wastewater problems at the source, achieving environmental friendliness in the molecular sieve synthesis process and significantly reducing production costs.

The present invention relates to a bioreactor for RNA in vitro transcription, a method for RNA in vitro transcription, a module for transcription of DNA into RNA and an automated device for RNA manufacturing. Furthermore, the use of a bioreactor for RNA in vitro transcription as described herein is part of the present invention. The present invention relates to an RNA in vitro transcription reactor designed to be operable in an automated manner under GMP compliant conditions. In particular, the RNA in vitro transcription reactor allows for repeated use of a DNA template for various RNA in vitro transcription reactions. Furthermore, the present invention relates to a device for RNA manufacturing comprising (a) a module for template DNA synthesis, (b) a module for transcription of DNA into RNA, the module comprising the RNA in vitro transcription reactor; and optionally (c) a module for RNA formulation.

The invention relates to the technical field of biological feature recognition, and discloses a fingerprint unified identity authentication platform which comprises a fingerprint registration module, a template synthesis module, a data storage module, a fingerprint comparison module and a dynamic threshold adjustment module. According to the unified fingerprint identity authentication platform, the fingerprint templates are stored in the database in a centralized manner in a hexadecimal data format through the unified fingerprint identity authentication platform, multi-system redundant storage is eliminated, the management complexity is reduced, unified encryption strategy and authority level-to-level management are supported, the risk of data leakage or tampering is prevented, and the user experience is improved. A user does not need to repeatedly register fingerprints in a plurality of systems, the operation process is simplified, the fingerprints are loaded to the memory space of the server in a binary data form based on multithreading, concurrent loading and comparison operation, and the fingerprint data are processed in parallel, so that the recognition efficiency and accuracy are improved.

The present application relates to the technical field of hierarchical pore molecular sieve materials, and discloses a preparation method of hierarchical pore FAU type and EMT molecular sieve with super-large pore volume. A silicon source such as sodium silicate, silica sol, orthosilicic acid, fumed silica and the like is mixed with an aluminum source such as aluminum powder, sodium aluminate, aluminum hydroxide, aluminum sulfate, aluminum isopropylate, tertiary butyl aluminum and the like to configure a gel with a certain proportion, and then additional silicon source and aluminum source are added to adjust the proportion, and crystallization is carried out at a certain temperature for a period of time. The mixture is separated by filtration, and then washed by water filtration. The obtained solid is dried to obtain the hierarchical pore molecular sieve material. The method provided by the present application has a simple synthesis process, does not need to use an organic template agent, has wide raw material selection, and has low production cost. The molecular sieve obtained by the present application has high total specific surface area and large external specific surface area (FAU type 834 m 2 / g and 138 m 2 / g, EMT type 591 m 2 / g and 199 m 2 / g), and super-large pore volume (FAU type 1.40 cm 3 / g, EMT type 0.92 cm 3 / g).

The invention belongs to the technical field of biology, and particularly relates to terminal deoxyribonucleotide transferase and application thereof. The present invention provides a terminal transferase or a mutant thereof; wherein both the terminal transferase and the terminal transferase mutant have catalytic activity independent of template synthesis of nucleic acid molecules, the total cost of synthesizing custom nucleic acid is reduced, and nucleic acid can be synthesized faster, cheaper and higher in quality even if modified nucleotide is used; especially, compared with the terminal transferase, most of the terminal transferase mutants have The present invention relates to a primer chain having a higher catalytic activity (i.e., the activity of catalyzing the addition of nucleotides (preferably 3 '-O-allyl modified nucleotides, preferably RNA) to the primer chain) and / or a wider extension / incorporation ability (i.e., more species of nucleotides (preferably 3'-O-allyl modified nucleotides, preferably RNA) can be incorporated to the primer chain).

The present disclosure discloses a method for preparing a spatial chip, a spatial chip, and a use thereof. The method comprises: hybridizing to attach a single-stranded nucleic acid molecule to a surface of a solid-phase substrate, the single-stranded nucleic acid molecule comprising a first fixed sequence, a spatial barcode sequence, a sequencing primer binding sequence and a second fixed sequence, a first probe being attached to the surface of the solid-phase substrate of the chip, and the first probe comprising a complementary sequence of the first fixed sequence and a complementary sequence of the second fixed sequence; ring closing, causing the single-stranded nucleic acid molecule to form a circular nucleic acid molecule; rolling circle amplification; sequencing, to obtain sequence information of the spatial barcode sequence; synthesizing a single-stranded nucleic acid molecule by using the rolling circle amplification product as a template, to obtain a single-stranded nucleic acid molecule library; and fixing and clustering, fixing the single-stranded nucleic acid molecule library on the surface of the solid-phase substrate to form a single-stranded nucleic acid molecule cluster. According to the present disclosure, a chip having a nucleic acid probe carrying spatial position coordinate information can be prepared.

The invention discloses an oligonucleotide modified carrier as well as a preparation method and application thereof, and particularly relates to a polymer, a polymer modified carrier, an oligonucleotide modified carrier as well as a preparation method and application thereof. In the oligonucleotide modified vector, oligonucleotide can be connected to the surface of the vector in a specific connection form, and the oligonucleotide is not easy to fall off and has good stability while the oligonucleotide load is relatively high, so that the oligonucleotide modified vector can be successfully applied to DNA template-free synthesis reaction and the like.

The invention belongs to the technical field of supramolecular chemistry, and particularly relates to a cerium-based metal organic framework (Ce-MOF) material as well as a preparation method and application thereof. According to the ultrasound-assisted soft template synthesis method disclosed by the invention, the reaction of metal ions and organic ligands on the surface of the soft template is accelerated by virtue of ultrasound within a short time, and the Ce-MOF sphere with a core-shell structure is synthesized in one step. The method disclosed by the invention is simple and easy to implement and short in time consumption, the whole synthesis process only needs 5-10 minutes, the reaction condition is mild, and the method can be completed at room temperature. Besides, the Ce-MOF material with the hollow structure synthesized by the method disclosed by the invention reserves the antioxidant enzyme activity of Ce-MOF and can effectively remove active oxygen, and meanwhile, the hollow structure of the Ce-MOF material provides an efficient drug loading potential, so that the Ce-MOF material has a wide application prospect in treatment of nerve injury diseases.

The invention provides organic-inorganic hybrid mesoporous silica nanoparticles, and a self-template synthesis method and application thereof. A long-chain alkylamino group is connected to the surface of a silicon dioxide nanoparticle, the long-chain alkylamino group is a dialkyl-substituted alkylamino group, the length of an alkyl substituent on a nitrogen atom in an amino group is 1-3, and a mesoporous structure can be formed. According to the invention, the long-chain alkylamino group is used as a self-template agent, and the mesoporous silica nanoparticle material is synthesized by a reversed-phase microemulsion method. The organic-inorganic hybrid mesoporous silica nanoparticle material can be used as an adsorbent to remove various organic pollutants in water, and has large adsorption capacity and high adsorption efficiency.