14results about How to "Fluorescence enhancement" patented technology

The invention belongs to the field of low-dimensional semiconductor material micro-nano photoelectric integration, and particularly relates to a method for enhancing TMDs fluorescence based on a dark state energy transfer mechanism, a perovskite-TMDs heterojunction is obtained, then excitation is performed through a two-photon pump light source, dark state energy is accumulated, and space-prohibited dark state energy transfer is induced, so that the TMDs fluorescence is enhanced; the perovskite-TMDs heterojunction comprises a perovskite nanosheet and TMDs compounded on the surface of the perovskite nanosheet, and dark exciton state energy bands of perovskite and TMDs are matched. The invention also provides application of the method to improvement of fluorescence intensity in a device. The invention provides a brand new TMDs fluorescence enhancement thought, the TMDs and the perovskite are innovatively used for constructing a heterojunction system, and through combined control of heterojunction energy band matching components constructed by the TMDs and the perovskite and excitation of the two-photon pump light source, dark-state energy transfer can be induced, and the fluorescence enhancement effect of the two-photon pump light source is improved. Therefore, fluorescence enhancement of TMDs is realized based on a dark state energy transfer mechanism.

The invention relates to a preparation method and application of selenium-doped carbon quantum dots. The selenium-doped orange fluorescent carbon quantum dots are successfully prepared by treating a mixture of mangosteen peel, ethanol and sodium selenite through a hydrothermal method. Based on the excellent fluorescence property of the carbon quantum dots, the Cr < 2 + > can quench the fluorescence of the selenium-doped carbon quantum dots, so that the Cr < 2 + > can be detected. Meanwhile, the carbon quantum dot is found to be responsive to disease micromolecule glutathione, that is, glutathione can enhance fluorescence of the carbon quantum dot. By utilizing the concentration difference of glutathione in normal cells and cancer cells, the carbon quantum dots can realize the recognition of tumor cells. The orange fluorescent carbon quantum dot provided by the invention is prepared from easily available raw materials, and is safe and non-toxic. The prepared carbon quantum dot has stable fluorescent property, uniform particle size of 4.2 + / -0.5 nm, and relative quantum yield of 34.35%, can be imaged in cells, has GSH detection limit of 27.16 umol / L, and can identify tumor cells and normal cells.

The invention provides a near-infrared response type asymmetric polymethine cyanine dye as well as a preparation method and application thereof. The dye has a structure as shown in a general formula I. The probe is combined with H < + > through an azaindole part in an asymmetric polymethine cyanine dye structure, so that rapid and reversible response to pH change is realized. The probe has high sensitivity and high specificity on pH, the pKa value is 6.46, the pH response linear range is 5.0-7.0, and the probe is suitable for monitoring the pH value in mitochondria. Due to inherent positive charges of the structure, the material can be gathered in mitochondria in a targeted manner and is kept stable in an autophagy process. The probe has the characteristics of high sensitivity, reliability and visualization, and is suitable for the fields of basic scientific research, drug action mechanism evaluation, cell physiological function and disease model analysis, environmental pollutant effect monitoring, regenerative medicine and the like. In addition, the probe is good in biocompatibility and high in light stability, is compatible with common fluorescence imaging equipment, and is convenient to popularize and apply.

The present invention discloses a preparation method and biological application of a fluorescent probe with lipid droplet recognition and polarity sensitivity. The fluorescent probe CN obtained by the present invention can efficiently and accurately target lipid droplets. The preparation method adopted by the molecule is simple and the conditions are mild. The maximum emission wavelength of the fluorescent probe is at 724nm, which can realize near-infrared I zone fluorescence imaging, and the maximum Stokes shift can reach about 190nm. The maximum emission wavelength and the maximum excitation wavelength have a large wavelength difference, thereby solving the problem of autofluorescence quenching in the measurement, and the background interference is low and the detection sensitivity is high. In the environment inside the lipid droplet, the fluorescence of the probe CN is significantly enhanced, and it can efficiently and accurately target the lipid droplets, detect changes in the number of lipid droplets and changes in polarity in cells. Imaging experiments at the cell and tissue animal level show that it has good biosafety and good photostability. The probe CN provides a reliable tool for further studying the dynamic changes of lipid droplets and the occurrence and development of Parkinson's disease.

The present invention relates to the preparation and application of a targeted glutathione fluorescent probe based on cyanine dyes, and the structural formula of the fluorescent probe is: The present invention provides a preparation method for synthesizing the fluorescent probe using cyanine fluorescent dyes, D-biotin, 4-hydroxy-4-nitroazobenzene, etc. as raw materials; the fluorescent probe is a glutathione fluorescent probe with near-infrared emission, high selectivity and the ability to target cells. First, the fluorescent probe shows high sensitivity to GSH, and the fluorescence is significantly enhanced after the probe reacts with GSH; secondly, the fluorescent probe shows good selectivity to GSH and is not interfered by other common inorganic ions, reactive oxygen, reactive nitrogen, reactive sulfur, amino acids, and biothiols; in addition, the fluorescent probe has been successfully used for GSH imaging in cells and can detect the glutathione level in cells.

The invention provides a method for improving the fluorescence intensity of a two-dimensional polymer with excitation wavelength dependence, which comprises the following steps: (1) adding the two-dimensional polymer with excitation wavelength dependence into an organic solvent, stirring to obtain a uniform and transparent solution, adding fluorescent molecules and a catalyst into the uniform and transparent solution, and stirring to obtain a transparent solution; carrying out esterification or amidation reaction on the two-dimensional polymer with excitation wavelength dependence and fluorescent molecules; and (2) after the reaction is finished, precipitating, dialyzing, filtering and drying to obtain the product. Traditional fluorescent molecules are introduced into the two-dimensional polymer with excitation wavelength dependence to change the molecular aggregation state of the two-dimensional polymer and limit intramolecular rotation or intramolecular vibration, so that non-radiation attenuation is reduced, fluorescence of the two-dimensional polymer with excitation wavelength dependence is remarkably enhanced, and the fluorescence intensity of the two-dimensional polymer with excitation wavelength dependence is improved. According to the present invention, the excitation wavelength dependence is maintained in the relatively wide excitation wavelength range, the original blue and green are enhanced, the red fluorescence can be obtained through adjustment, and the red fluorescence intensity is correspondingly enhanced;

The utility model provides a night measuring tape, which comprises a shell, a rotating shaft rotationally arranged in the shell, a tape band wound on the rotating shaft, and a brake assembly separably abutted against the tape band, the brake assembly is slidably arranged on the side part of the shell, and scale marks on the tape band are printed by fluorescent materials; a lamp bead is arranged at the position, above the ruler belt, of the shell, a battery connected with the lamp bead is arranged in the shell, a solar panel connected with the battery is arranged on the outer side of the shell, a lamp bead switch is arranged between the lamp bead and the battery, and one end of the lamp bead and one end of the battery are connected through a wire. According to the night measuring tape provided by the utility model, the battery is charged through the solar panel, and the battery supplies power to the lamp beads on the shell in a dark environment so as to carry out auxiliary irradiation on the tape band, so that the fluorescent time of the fluorescent scale marks on the tape band is prolonged, the luminous effect of the tape band is better, the duration is longer, and the applicability of the night measuring tape is stronger.

The invention discloses a preparation method and application of a gold nanocluster / antigen aggregate, and the preparation method comprises the following steps: dropwise adding a Prot solution into ATT-AuNCs, incubating to synthesize Proton-AuNCs, carrying out centrifugal treatment, and storing in a dark place to prepare the Proton-AuNCs; the preparation method comprises the following steps: mixing Proton-AuNCs with CAR-BSA (Chimeric Antigen Receptor-Bovine Serum Albumin) to prepare a gold nano cluster / antigen aggregate; the pairing of FeNPs and the aggregate enables the FONLFA detection system to achieve high sensitivity, the sensitivity of the FONLFA detection system is respectively improved by 5 times and 100 times compared with the sensitivity of an off mode, the sensitivity of the FONLFA detection system is respectively improved by 5 times and 200 times compared with the sensitivity of a traditional colorimetric mode, and the FONLFA detection system can be used for carrying out naked eye detection on a large batch of samples.

A light-emitting material, a light-emitting device, an electronic apparatus, and an electronic device are provided. The luminescent material has a form in which an electron-donating group and an electron-withdrawing group are attached to the benzene and the electron-withdrawing group is located ortho to the electron-donating group.

The present invention discloses a method for preparing a hexagonal boron nitride quantum light source assisted by metal nanoparticles, comprising the following steps: transferring a two-dimensional material hexagonal boron nitride sheet to a PDMS sheet substrate, dotting metal nanoparticles, and then transferring the hBN sheet and the metal nanoparticles on the polyPDMS substrate as a whole to a silicon dioxide substrate; using a laser to activate defects in the hBN sheet to generate a quantum light source, and under the action of the metal nanoparticles, the quantum light source is enhanced. The present invention generates a localized surface plasmon resonance effect under laser excitation, and the hot electrons generated enrich carbon-related defects to form a quantum light source and generate fluorescence, so that the luminescence and Raman scattering intensity of the quantum light source are enhanced, and the metal nanoparticles cause strain in hBN, and the strain reduces the migration barrier in the process of carbon-related defect enrichment, further enhancing the fluorescence.

The invention discloses a preparation method of aggregation-induced luminescence gold nanoclusters and its effect on Ag + , Hg 2+ , H2O2, GSH detection, including the synthesis of dual-ligand aggregation-induced emission gold nanoclusters, and their application in fluorescence detection of silver ions and mercury ions in aqueous solution, and continuous colorimetric detection of hydrogen peroxide and glutathione, and the invention of a material that can be used for colorimetric-fluorescence dual-mode multi-target detection. The probe completes the simultaneous detection of these two metal ions based on the aggregation-induced emission mechanism. The gold nanoclusters of the present invention also have excellent peroxidase-like activity and can catalyze the oxidation of 3,3',5,5'-tetramethylbenzidine. Glutathione, as a strong reducing agent, can decolorize the system and remove free radicals generated during the reaction, thereby realizing continuous colorimetric detection of hydrogen peroxide and glutathione. The invention realizes dual-modal multi-target detection and has broad application prospects.

The invention belongs to the field of fluorescent probes, and discloses a novel rhodamine Al < 3 + > fluorescent probe as well as a preparation method and application thereof. The method comprises the following steps: synthesizing an intermediate R from cyanuric chloride serving as a framework and p-hydroxybenzaldehyde, synthesizing rhodamine B hydrazide from rhodamine B and hydrazine hydrate under the protection of nitrogen, and finally synthesizing the final product probe R1 from the intermediate R and the rhodamine B hydrazide. After 10 times of KSCN is added into the probe R1 as a color developing agent, the interference of Fe < 3 + >, Cu < 2 + > and Zn < 2 + > ions is masked, and the probe R1 shows high sensitivity and selectivity to Al < 3 + >. Furthermore, the lowest detection limit of Al < 3 + > is calculated to be 0.1 mu mol / L through fluorescence titration test. Therefore, the probe R1 prepared by the invention can realize high-selectivity fluorescent recognition on Al < 3 + > ions, and can be used as a fluorescent probe for detecting metal ions to be applied to the related fields of environment detection, biomedical treatment, agriculture and the like.

The application discloses a kind of nanodiagnosis and treatment materials based on water-soluble columnar aromatic hydrocarbon.The application designs and synthesizes a new type of columnar [5] aromatic hydrocarbon WP5, the columnar [5] aromatic hydrocarbon WP5 has water-soluble quality and contains rigid structure cavity macrocyclic molecule, can occur with porphyrin molecule host-guest complexation interaction.Using the water-soluble columnar [5] aromatic hydrocarbon WP5 and porphyrin guest molecule host-guest complexation, can realize cavity protection porphyrin compound inhibits the accumulation between porphyrin molecules, improves its stability and the ability of producing singlet oxygen, while enhancing fluorescence, and improve porphyrin compound light stability.

The invention discloses a gel bis-pyrenyl fluorescent probe, a preparation method thereof and application of the gel bis-pyrenyl fluorescent probe in detection and removal of cadmium, and the probe can be complexed with Cd < 2 + > in a 5% DMSO solvent, so that the fluorescence of the probe is enhanced. The probe molecule PYRX can qualitatively and quantitatively detect Cd < 2 + > in a real sample, has the characteristics of quick response and low detection limit, and has better stability under a wide pH condition, so that the probe molecule PYRX has important application value in the fields of biochemistry and the like. Meanwhile, a probe PYRX-loaded agarose gel PYRX-aga is constructed, and Cd < 2 + > in a food sample can also be qualitatively and quantitatively detected. As the agarose gel has a large number of apertures, the PYRX-aga is applied to adsorption removal of Cd < 2 + > in a solution. In the removal process, the PYRX-aga device can show a good fluorescence / colorimetric effect, and the removal efficiency can reach 96% or above. # imgabs0 #