87 results about "Liposome suspension" patented technology

The invention discloses a preparation method of composite lipopeptide capable of preventing high-temperature inactivation, and belongs to the technical field of bioactive peptide preparation. The preparation method comprises the following steps: mixing and dissolving a modified phospholipid compound, cholesterol and chloroform, and performing rotary evaporation to obtain a lipid film; mixing and shaking the lipid film, the lipopeptide mixture and a phosphate buffer solution, and performing ultrasonic treatment to obtain a composite lipopeptide liposome suspension; and carrying out spray drying on the composite lipopeptide liposome suspension to obtain composite lipopeptide powder. A lipopeptide mixture obtained through fermentation is mixed with a modified phospholipid compound and cholesterol, a high-temperature-resistant composite lipopeptide liposome suspension is constructed, and after high-temperature spray drying, the obtained composite lipopeptide still has good antibacterial activity.

The invention relates to a composite dissoluble microneedle gel patch mask and a preparation method thereof. Comprising the following steps: (1) preparing a lipid oil phase solution and a water phase solution; mixing the lipid oil phase solution with the water phase solution to obtain a liposome suspension; (2) preparing recombinant collagen hydrogel; (3) adding the tea polyphenol and the lipidosome suspension into the recombinant collagen hydrogel to obtain composite gel; and (4) injecting the composite gel into a mold, carrying out vacuum drying, and demolding to obtain the composite dissoluble microneedle gel patch mask. According to the composite dissoluble microneedle gel patch mask provided by the invention, the tea polyphenol, the plant exosome and the liposome carrier are integrated into the microneedle gel patch mask for the first time, the oxidation resistance is improved by utilizing the tea polyphenol, the inflammation resistance is improved by utilizing the plant exosome, the transdermal effect is enhanced by utilizing the liposome, the transdermal efficiency reaches 35.2-42.5 mu g / cm < 2 > / h, the medical beauty effect is obvious, and the application prospect is wide. The obvious advantages are realized in the aspects of safety, transdermal efficiency and repairing effect.

The invention relates to the technical field of compositions, and provides a hair-blacking and hair-loss-preventing traditional Chinese medicine and trace element composition and a preparation method and application thereof.The preparation method includes the steps that traditional Chinese medicinal materials with the effects of nourishing the liver and kidney, nourishing blood and growing hair are selected, and a multi-step water extraction and alcohol extraction combined technology is adopted to prepare high-purity traditional Chinese medicine extract dry powder; meanwhile, soluble trace elements such as zinc, iron and copper are selected, the optimal proportion of the soluble trace elements, the iron and the copper is determined through a response surface optimization algorithm, and a synergistic activity system is formed. Then, a mixture of traditional Chinese medicines and trace elements is dissolved in a buffer solution, and the mixture is entrapped in lipidosome composed of phospholipid and cholesterol through an ultrasonic nanocrystallization process, so that a stable nanoscale composite carrier is prepared; and finally, uniformly mixing the liposome suspension with a gel matrix to prepare the liposome gel dosage form for scalp external use. The problems of random component proportion, poor permeability and lack of mechanism verification in the prior art are solved.

The invention belongs to the technical field of collagen skin care products, and provides an oil-soluble recombinant collagen bionic nano-liposome delivery system and a preparation method and application thereof.The preparation method comprises the steps that A, a buffer solution with the pH being 3.8-4.2 is added into a lipid film, oscillation and ultrasonic treatment are conducted, and blank liposome is obtained; b, dissolving the integrin alpha6beta4 transmembrane protein by using a buffer solution with the pH value of 7.2-7.4; adding the transmembrane protein solution into blank lipidosome, oscillating and incubating at 37 DEG C, and performing ultrafiltration and centrifugation to obtain lipidosome suspension; c, dropwise adding the recombinant collagen solution into the liposome suspension, stirring at a constant temperature of 40 + / -2 DEG C, and performing ultrasonic treatment to obtain a collagen liposome suspension; and D, homogenizing the collagen liposome suspension at high pressure, and performing ultrafiltration purification to obtain the purified collagen liposome suspension. Through the technical scheme, the problems of low collagen encapsulation efficiency and low transdermal efficiency in the prior art are solved.

The invention discloses a preparation method of an external skin care product, belongs to the technical field of cosmetics, and aims to solve the problems of weak effect of a single target spot, poor transdermal permeability of active ingredients and easy inactivation in the prior art. The method comprises the following steps: preparing a composition containing an A-phase licorice extract and a B-phase bearberry extract, wherein the mass ratio of the A phase to the B phase is 1: (1.5-3); the preparation method comprises the following steps: preparing a liposome suspension of which the entrapment rate is greater than or equal to 88% and the particle size is 50-200nm by using a hydrogenated soybean lecithin-phytosterol-ceramide composite membrane material through a membrane dispersion-high-pressure homogenization method; mixing with auxiliary materials to prepare essence or emulsion; and smearing according to 0.5-1mg / cm < 2 > to realize double-target synergistic blackening inhibition. According to the scheme, the melanin inhibition rate reaches 54%-62% through the double-target design in cooperation with composite membrane material liposome delivery, the active ingredient retention rate is larger than or equal to 89% after storage at 45 DEG C for 6 months, the transdermal rate is increased to 40%-48%, and the method is mild, low in irritation and remarkable in application value.

The invention belongs to the technical field of development of nintedanib preparations, and particularly relates to a nintedanib suspension preparation, a preparation method thereof and application of the nintedanib suspension preparation in treatment of pulmonary fibrosis. The preparation method comprises the following steps: dissolving a phospholipid material in an organic solvent at 50-70 DEG C, and preserving heat to obtain a solution A; dissolving inorganic salt in water for injection, and preserving heat to obtain a solution B; dissolving the ethanesulfonic acid nintedanib in the water for injection to obtain a solution C; dissolving an osmotic pressure regulator in the water for injection to obtain a solution D; mixing the solution A and the solution B by using microfluidics to obtain a suspension E; filtering through a tangential flow membrane to remove the organic solvent in the suspension E, adding the solution C, keeping the temperature for a period of time, adding the solution D as required, and uniformly mixing; and filtering the suspension with a sterilization filter to obtain the nintedanib suspension preparation. The liposome suspension prepared from the nintedanib prepared by the invention is stable in physical state and excellent in slow release effect, is suitable for low-dose inhalation administration in the lung, and is beneficial to improving the treatment effect and reducing side effects.

The invention discloses retinol retinoate-ceramide lipidosome modified by hyaluronic acid and salts thereof and a preparation method of the retinol retinoate-ceramide lipidosome, and belongs to the technical field of cosmetic nano-carriers. The preparation method comprises the steps that retinol retinoate-ceramide lipidosome is prepared, hyaluronic acid is pretreated, and the hyaluronic acid and the salts thereof are combined to prepare the retinol retinoate-ceramide lipidosome. The liposome is modified by hyaluronic acid and salts thereof in a dual-mechanism manner, and purification and stabilization are realized; the preparation method of the retinol retinoate-ceramide liposome comprises the following steps: dissolving phospholipid, a functionalized lipid component with positive ions and amino functional groups, cholesterol, retinol retinoate and ceramide in absolute ethyl alcohol to serve as an organic phase, taking a phosphate buffer solution as a water phase, dropwise adding the organic phase into the water phase under a stirring condition, stirring, performing ultrasonic treatment, washing, and drying to obtain the retinol retinoate-ceramide liposome. Performing membrane extrusion to obtain a liposome suspension; the retinol retinoate-ceramide liposome modified by hyaluronic acid and salts thereof prepared by the invention has excellent stability, skin transmittance and anti-aging and moisturizing effects, also has low irritation, and is suitable for anti-aging cosmetics.

The invention relates to a eugenol liposome / chitosan / polyoxyethylene hydrophobic electrospun fibrous membrane and a preparation method thereof, and the method comprises the following steps: based on soybean lecithin and cholesterol, adding eugenol and Tween 80, preparing a lipid solution, injecting the lipid solution into water or a phosphate buffer solution, and preparing an eugenol liposome suspension with the eugenol embedding rate greater than 88%; preparing a cross-linked chitosan acetic acid solution based on chitosan and a cross-linking agent; preparing a polyoxyethylene acetic acid solution, and mixing the cross-linked chitosan acetic acid solution with the polyoxyethylene acetic acid solution to obtain a base material spinning solution; mixing the eugenol liposome suspension with a base material spinning solution to prepare a final spinning solution, and preparing the eugenol liposome / chitosan / polyoxyethylene hydrophobic electrospinning fiber membrane by adopting an electrostatic spinning method. The embedding rate of eugenol is high, the water contact angle of the fiber membrane can reach 90 degrees or above, the slow release effect on eugenol is good, and the antibacterial and fresh-keeping effects are improved.

The invention relates to an MPL composite adjuvant and a preparation method thereof, and belongs to the technical field of vaccine adjuvants. The composite adjuvant comprises neutral phospholipid, anionic phospholipid, cholesterol, monophosphoryl lipid A, QS-21 and surface modified lipid. The preparation method comprises the following steps: dissolving a lipid component in an organic solvent to form a mixed solution, evaporating the solvent to form a lipid film, adding a buffer solution, hydrating to form a liposome suspension, performing ultrasonic treatment to obtain small single-layer liposome, and mixing the small single-layer liposome with a QS-21 micelle solution. By introducing the surface modified lipid, the stability of the adjuvant is remarkably improved, the adjuvant is small in particle size, uniform in distribution, high in Zeta potential negative value and good in stability in a serum environment, can induce high-level antibody immune response, and is suitable for vaccine development.

The present invention provides an injectable magnesium-loaded lipid-based super-lubricating hydrogel microsphere, an articular cavity injection formulation, and a preparation method therefor. The method comprises the following steps: S1, preparing a magnesium-loaded multilamellar vesicular liposome suspension containing magnesium ions; S2, preparing an uncrosslinked magnesium-containing hydrogel; and S3, mixing the magnesium-loaded multilamellar vesicular liposome suspension with the magnesium-containing hydrogel to prepare hydrogel droplets, and carrying out crosslinking to form the magnesium-loaded lipid-based super-lubricating hydrogel microspheres. According to the present invention, the magnesium loading capacity of the lipid-based super-lubricating hydrogel microspheres can be greatly improved; on one hand, the biological function provided by the magnesium ions can be greatly prolonged, that is, the repair of cartilage by synovial liquid progenitor cells is continuously promoted; and on the other hand, the intrinsic lubricating effect of the lipid-based super-lubricating hydrogel microspheres can mitigate the further wear of newly formed cartilage; in addition, the magnesium ions can significantly promote the expression of PRG4 in chondrocytes, which not only facilitates the maintenance of chondrocyte homeostasis, but also further enhances articular lubrication.

The present invention provides liposome compositions containing sparingly soluble drugs that are used to treat life-threatening diseases. A preferred method of encapsulating a drug inside a liposome is by remote or active loading. Remote loading of a drug into liposomes containing a transmembrane electrochemical gradient is initiated by co-mixing a liposome suspension with a solution of drug, whereby the neutral form of the compound freely enters the liposome and becomes electrostatically charged thereby preventing the reverse transfer out of the liposome. There is a continuous build-up of compound within the liposome interior until the electrochemical gradient is dissipated or all the drug is encapsulated in the liposome. However, this process as described in the literature has been limited to drugs that are freely soluble in aqueous solution or solubilized as a water-soluble complex. This invention describes compositions and methods for remote loading drugs with low water solubility (<2 mg / mL). In the preferred embodiment the drug in the solubilizing agent is mixed with the liposomes in aqueous suspension so that the concentration of solubilizing agent is lowered to below its capacity to completely solubilize the drug. This results in the drug precipitating but remote loading is capability retained. The process is scalable and the resulting drug-loaded liposomes are characterized by a high drug-to-lipid ratios and predictable drug retention when the liposome encapsulated drug is administered in patients.

The application discloses a naringenin-antibacterial peptide composite liposome hydrogel and a preparation method and application thereof, and relates to the technical field of medicine, in particular to a naringenin-antibacterial peptide composite liposome hydrogel, which is prepared by the following steps: dissolving soybean lecithin, cholesterol and naringenin in a chloroform-methanol mixed solvent, rotary evaporation under reduced pressure, and vacuum solvent removal to obtain a naringenin-loaded lipid film; dissolving a bacillus subtilis antibacterial peptide in a PBS buffer solution to prepare a hydration solution, adding the lipid film to the hydration solution to rotate and hydrate to obtain a crude liposome suspension, and extruding the liposome suspension through polycarbonate membranes at room temperature to obtain a co-loaded liposome suspension; dispersing carbomer 940 in pure water, standing and swelling to prepare a gel matrix, mixing a humectant and a preservative into the gel matrix, and adding a neutralizing agent dropwise to adjust the pH value to 6.8-7.0; and mixing the liposome suspension and the gel matrix at room temperature, adding a PBS buffer solution to the mixture to prepare the naringenin-bacillus subtilis antibacterial peptide co-loaded liposome hydrogel, so as to solve the problems of skin irritation and bacterial drug resistance caused by long-term use of traditional acne treatment drugs.

The application provides a fermented vegetable oil and a preparation method thereof. The preparation method comprises the following steps: crushing a vegetable oil raw material and mixing the same with water to obtain a raw material solution; adding a biological enzyme to the raw material solution to perform enzymolysis, and after enzyme inactivation, an enzymolysis product is obtained; then, a fermentation bacteria is added to perform fermentation, sterilization and centrifugation to obtain an oily liquid; the oily liquid, 1,2-dilauroylphosphatidyl ethanolamine, a self-assembled structure regulator and propylene glycol are uniformly mixed, and then added into water to obtain a liposome suspension of the fermented vegetable oil; 3-thiophene malonic acid and / or flower acid are first added into the liposome suspension of the fermented vegetable oil, and then heparan sulfate and calcium chloride are added, and after reaction, the fermented vegetable oil encapsulated in a carrier is obtained. The preparation method of the fermented vegetable oil has good long-term stability, and can also improve the poor compatibility of the fermented vegetable oil with a water-based system.

The invention discloses drug-loaded hydrogel as well as a preparation method and application thereof, and belongs to the technical field of medical hydrogel preparation. The preparation method comprises the following steps: mixing a drug-loaded liposome suspension, a carboxymethyl chitosan aqueous solution, an oxidized sodium alginate aqueous solution and a madecassoside aqueous dispersion according to a weight part ratio of 1: 10: (2-5): (0.2-0.5), incubating at 45-50 DEG C for 2-3 hours, cooling to 25 DEG C, and standing for 6-8 hours to obtain the drug-loaded hydrogel. The preparation method comprises the following steps: loading a natural antibacterial composition into lipidosome to obtain a drug-loaded lipidosome suspension, mixing a carboxymethyl chitosan aqueous solution, an oxidized sodium alginate aqueous solution and a madecassoside aqueous dispersion, and carrying out cross-linking gel solidification to obtain the drug-loaded hydrogel with good antibacterial performance.

The invention provides a polypeptide modified phenol essential oil nano-liposome and a preparation method thereof, and the method comprises the following steps: (1) successively carrying out rotary evaporation, film hydration and ultrasonic dispersion on soybean lecithin, cholesterol and origanum vulgare essential oil to obtain an origanum vulgare essential oil nano-liposome suspension; and (2) sequentially mixing the origanum vulgare essential oil nano-liposome suspension and polylysine, and stirring to obtain the polypeptide modified phenolic essential oil nano-liposome. Soybean lecithin, cholesterol and origanum vulgare essential oil in a specific mass ratio and polylysine in a specific mass are self-assembled to form the nano-liposome in which antibacterial and antioxidant active substances are slowly released, growth and reproduction of escherichia coli and staphylococcus aureus can be effectively inhibited, meanwhile, main base materials of the nano-liposome are edible materials, and the nano-liposome has a good antibacterial and anti-oxidation effect. The product can be used for functional delivery of active substances and storage and preservation of fresh food, and meets the requirements of people for safe, nutritional and healthy diet and green and environment-friendly ideas.

The invention belongs to the technical field of cosmetic liposomes, and relates to a PDRN-silk fibroin compounded plant external vesicle liposome as well as a preparation method and application thereof. The preparation method of the liposome comprises the following steps: preparing plant external vesicles; mixing the active component with alcohol, adding a water-soluble silk fibroin solution, and stirring and shearing to obtain an active component-silk fibroin compound; dissolving hydrogenated phospholipid and cholesterol in an organic solvent, and carrying out rotary evaporation to remove the organic solvent to obtain a lipid membrane; adding a PDRN solution and water into the lipid membrane, stirring and mixing, adding plant external vesicles and an active ingredient-silk fibroin compound, stirring, shearing and emulsifying to obtain a primary emulsion; and carrying out high-pressure homogenization treatment on the primary emulsion to obtain a liposome suspension, and filtering to obtain the liposome. The prepared liposome is small in particle size, low in PDI, high in zeta potential absolute value, excellent in stability, good in heat resistance, excellent in transdermal performance, high in active ingredient delivery efficiency and excellent in skin repairing and anti-aging effects.

This invention discloses a compound preparation for treating vascular dementia and its preparation method. The active pharmaceutical ingredient consists of protopanaxadiol and gallic acid in a 1:1 mass ratio, and the dosage form is liposomes. The preparation method includes extraction, mixing, liposome preparation, and optional freeze-drying steps: protopanaxadiol and gallic acid are extracted and purified from ginseng rhizome and Terminalia chebula fruit, respectively. After mixing, they are combined with phospholipids and cholesterol to prepare a lipid membrane. The membrane is then homogenized by hydration, low-temperature ultrasound, and high-pressure microfluidic jet to obtain a homogeneous liposome suspension, which can be further freeze-dried into a freeze-dried powder. The 1:1 composition of protopanaxadiol and gallic acid targets multiple points on the pathological mechanism of vascular dementia, showing significant therapeutic effects and broad application prospects. This preparation uses a liposome dosage form to improve the bioavailability of the active ingredient, the preparation process parameters are controllable, the active ingredient has high purity, the liposomes have uniform particle size, high encapsulation efficiency, and good stability.

The invention belongs to the technical field of biological medicine, and particularly discloses a sprayable double-network hydrogel microsphere adhesive and a preparation method and application thereof.The preparation method comprises the following steps that liquid paraffin, sorbitan monooleate and polyoxyethylene sorbitan monolaurate serve as oil phases, and an emulsion is formed; fully mixing the emulsion with petroleum ether, centrifugally separating gel microspheres, freeze-drying, and storing in a dark place; preparing a ropivacaine liposome; and adding ultrapure water into the lipid membrane to obtain a primary liposome suspension, adsorbing the liposome by using the hydrogel microspheres, and collecting the obtained dual-network hydrogel microspheres. According to the sprayable double-network hydrogel microsphere adhesive as well as the preparation method and the application thereof, prepared hydrogel microspheres can quickly and consistently cover irregular wounds, and liposome loaded with ropivacaine provides continuous analgesic release, so that the painful wound healing effect is improved. By simultaneously solving the problems of maintaining continuous adhesion and prolonging pain relief in blood-saturated wounds.

The invention belongs to the technical field of liposome modification, and particularly relates to a method for modifying stable light protection active collagen peptide liposome by adopting sodium alginate. Comprising the following steps: (1) adding a photoprotection active collagen peptide solution into a lipid film for hydration, and homogenizing to obtain a liposome suspension; and (2) dropwise adding a sodium alginate aqueous solution into the liposome suspension obtained in the step (1), mixing and stirring, and standing to obtain the photo-protection active collagen peptide liposome. The liposome prepared by the invention has excellent slow release performance, storage stability and gastrointestinal tract digestion stability, can stably release light in vivo for a long time to protect active collagen peptide, and can play a role in light protection for a long time; meanwhile, the preparation method is green and environment-friendly, the stability and bioavailability of the photoprotection active collagen peptide are greatly improved, and a brand new technical direction is opened up for research and development of cosmetics and medical products in the field of photoprotection.

The invention provides a rare ginsenoside nano-liposome, a preparation method thereof and application of the rare ginsenoside nano-liposome in improving sleep and relieving physical fatigue. The preparation method comprises the following steps: (1) preparing a lactoferrin solution; (2) preparing a rare ginsenoside solution; (3) preparing a thin film; (4) adding a rare ginsenoside solution into the film, and carrying out ultrasonic treatment to obtain a rare ginsenoside crude liposome suspension; (5) slowly dropwise adding the lactoferrin solution into the rare ginsenoside crude liposome suspension, and stirring for cross-linking reaction to obtain a rare ginsenoside crude liposome solution; and (6) carrying out high-pressure micro-jet nano-homogenization on the rare ginsenoside crude liposome solution to obtain the rare ginsenoside nano-liposome. The invention solves the technical problems of low safety, poor stability, low targeting property and low bioavailability of the traditional nano-liposome, and belongs to the technical field of functional foods.

The invention belongs to the technical field of biological medicines, and particularly discloses a liposome containing a natural extract composition as well as a preparation method and application of the liposome. The method for preparing the liposome containing the natural extract composition comprises the following steps: S1, adding the grape, poria cocos and apple extract, cholesterol and lecithin into an organic solvent, heating, stirring and dissolving to obtain an organic phase; adding the soybean extract and the ginseng extract into ultrapure water, heating and stirring to obtain a water phase; and S2, slowly adding the organic phase into the water phase while stirring, continuing stirring and emulsifying, and removing the organic solvent by reduced pressure distillation to obtain a liposome suspension. And S3, filtering the suspension, adding a freeze-drying protective additive, and freeze-drying to obtain the liposome. The invention also covers the application of the liposome in treating renal failure. In the invention, through the combination of natural extracts and the combination of a specific composition and liposome administration, the components generate a synergistic effect, and the pharmaceutical composition has a remarkable treatment effect on renal failure, especially chronic renal failure.

The invention discloses a preparation method of prodigiosin liposome, which comprises the following steps: S1, lipid phase emulsification: mixing phospholipid and cholesterol according to the mass ratio of (2-5): 1 to form a lipid phase with the concentration of 10-30 g / L, and emulsifying the lipid phase in a buffer solution with the volume ratio of 1: (3-5) to form a preliminary lipid phase emulsion; s2, mixed emulsion ultrasonic treatment: dropwise adding a prodigiosin alcoholic solution into the lipid phase preliminary emulsion to form a mixed emulsion, and sequentially carrying out emulsification and ultrasonic dispersion to obtain a dispersion liquid; s3, gradient extrusion and refinement: carrying out gradient extrusion on the dispersion liquid in filter membranes with the pore diameters of 180-260 nm, 140-160 nm and 80-120 nm in sequence to obtain a prodigiosin liposome suspension; and centrifuging and purifying to obtain the prodigiosin liposome. The prodigiosin liposome prepared by emulsifying and combining ultrasonic dispersion and gradient extrusion processes is uniform in particle size and high in encapsulation efficiency and stability.

The invention provides an injectable magnesium-loaded lipid-based super-lubricating hydrogel microsphere, an articular cavity injection preparation and a preparation method of the injectable magnesium-loaded lipid-based super-lubricating hydrogel microsphere. The method comprises the following steps: S1, preparing a magnesium-loaded multilayer vesicular liposome suspension containing magnesium ions; s2, preparing a non-crosslinked magnesium-containing hydrogel; and S3, mixing the magnesium-loaded multilayer vesicular liposome suspension and the magnesium-containing hydrogel to prepare hydrogel liquid drops, and carrying out crosslinking to form the magnesium-loaded lipid-based super-lubricating hydrogel microspheres. The magnesium loading amount of the lipid-based super-lubrication hydrogel microspheres can be greatly improved; on one hand, the biological function provided by magnesium ions can be greatly prolonged, that is, repairing of cartilage by synovial fluid progenitor cells is continuously promoted; on the other hand, the self-lubricating effect of the lipid-based super-lubricating hydrogel microspheres can reduce the secondary wear of the new cartilage; in addition, magnesium ions can remarkably promote expression of cartilage cells PRG4, so that the stable state of the cartilage cells can be maintained, and joint lubrication can be further enhanced.

The invention discloses a compound feed additive and a preparation method thereof, and belongs to the technical field of feed. The preparation method comprises the following steps: mixing a bacteria-loaded liposome suspension, a polysaccharide extract, modified stevioside and an emulsifier according to a weight part ratio of 1: (0.03-0.4): (0.1-0.2): 0.01, and carrying out a stirring reaction at 30 DEG C for 20-24 h to obtain a microcapsule emulsion; and carrying out spray drying treatment on the microcapsule emulsion to obtain the compound feed additive. Bacillus subtilis and bifidobacterium animalis are used as core strains in the compound feed additive, the core strains are prepared into a bacterium-loaded liposome suspension, then the bacterium-loaded liposome suspension is mixed with a polysaccharide extract, modified stevioside and an emulsifier for reaction, and finally high-temperature spray drying treatment is performed to obtain the compound feed additive. The compound feed additive capable of improving feed intake and weight gain is obtained.

The invention relates to a novel liposome polydopamine nanoparticle and application thereof in treating renal cell carcinoma. The preparation method of the nanoparticles comprises the following steps: adding liposome into si-circPVT1 dissolved in ribonuclease-free water, and carrying out vortex mixing to form a si-circPVT1 liposome suspension; dispersing the dopamine PDA modified liposome into a Tris-HCl solution, then adding dopamine hydrochloride, and stirring to obtain dopamine PDA modified liposome; then dispersing in a streptavidin solution, and fully oscillating at 4 DEG C under a dark condition to obtain a compound; and mixing the complex with a biotin-labeled MUC12 antibody solution for incubation, and washing the obtained suspension with a PBS (Phosphate Buffer Solution) to obtain the novel liposome polydopamine nanoparticles marked as SCLPM-NPs. The product prepared by the invention realizes targeted delivery of kidney cell cancer cell lines, and the SCLPM-NPs can effectively target tumor tissue parts and inhibit the growth of kidney tumors.

There is provided a liposomal suspension comprising transmembrane pH-gradient liposomes in an aqueous solution, the suspension comprising citric acid anhydrous and lipids in a ratio of (3.54 g / L) / (6.49 g / L) along with a kit for preparing the liposomal suspension, uses of the liposomal suspension and kits for treating an ammonia- or ammonia methylated analog-associated disease or disorder, or a symptom thereof; endotoxemia; or endotoxemia-associated disease or disorder or a symptom thereof, in a subject in need thereof. Also provided are a system and method for intraperitoneally administering the liposomal suspension to a subject in need thereof.

The application discloses a eugenol liposome / chitosan / polyethylene oxide hydrophobic electrospun fiber membrane and a preparation method thereof. The method comprises the following steps: based on soybean lecithin and cholesterol, adding eugenol and Tween 80 to prepare a lipid solution, injecting the lipid solution into water or a phosphate buffer to prepare eugenol liposome suspension with an eugenol embedding rate greater than 88%; based on chitosan and a crosslinking agent, preparing a crosslinked chitosan acetic acid solution; preparing a polyethylene oxide acetic acid solution, mixing the crosslinked chitosan acetic acid solution and the polyethylene oxide acetic acid solution to obtain a base spinning solution; mixing the eugenol liposome suspension with the base spinning solution to prepare a final spinning solution, and adopting an electrospinning method to prepare the eugenol liposome / chitosan / polyethylene oxide hydrophobic electrospun fiber membrane. The eugenol embedding rate is high, the water contact angle of the fiber membrane can reach more than 90 degrees, the slow-release effect of the eugenol is good, and the antibacterial and fresh-keeping effects are improved.

The present disclosure provides the use of a liposome suspension comprising transmembrane pH gradient liposomes in the treatment of acute hyperamminemia crisis (HAC) associated with innate metabolic error (IEM) in a subject, wherein the treatment comprises intraperitoneal administration of the liposome suspension to the subject and removal of a dialysate containing ammonia-loaded liposomes from the subject.

The invention discloses a preparation method and application of a wrapped glass pigment, belongs to the technical field of cosmetics, and provides the preparation method of the wrapped glass pigment. Comprising the following steps: weighing lecithin, choline phospholipid-polyethylene glycol-choline phospholipid and choline phospholipid-polyethylene glycol-cholesterol, and dissolving in absolute ethyl alcohol to obtain a phospholipid-cholesterol ethanol solution; performing ultrasonic treatment until no particles or insoluble substances can be seen by naked eyes, dissolving hydroxypropyl tetrahydropyrantriol in a phosphate buffer solution, and stirring under a 35-45 DEG C water bath condition after fully dissolving; the preparation method comprises the following steps: sucking a phospholipid-cholesterol ethanol solution by using an injector, dropwise adding the phospholipid-cholesterol ethanol solution into a hydroxypropyl tetrahydropyrantriol solution, stirring to form a liposome suspension, carrying out rotary evaporation on the suspension to remove ethanol, then carrying out ultrasonic dispersion under the condition of ice-water bath, and finally extruding to pass through a film to obtain the liposome wrapping the glassine. The glassine is wrapped by the cyclic polyethylene glycol modified lipidosome, so that the transdermal rate of the glassine can be remarkably improved.