Method for preparing self-assembly ketoprofen liposome by electrostatic spinning technology

An electrospinning technology and ketoprofen lipid technology are applied in the field of preparation of pharmaceutical liposomes, which can solve the problems of limited application, poor stability of liposomes, etc., and achieve simple operation, less time-consuming, good degradability and The effect of biocompatibility

Inactive Publication Date: 2012-10-03
DONGHUA UNIV
View PDF6 Cites 8 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the poor stability of liposomes and the residue of toxic organic solvents in the preparation process limit its application and development.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for preparing self-assembly ketoprofen liposome by electrostatic spinning technology
  • Method for preparing self-assembly ketoprofen liposome by electrostatic spinning technology
  • Method for preparing self-assembly ketoprofen liposome by electrostatic spinning technology

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] (1) Put the mixture of chloroform and N,N-dimethylacetamide (DMAc) at a volume ratio of 4:1 into the reaction vessel;

[0031] (2) Slowly add the weighed PVP, PC and ketoprofen powder into the above-mentioned reaction vessel under stirring conditions, and continue to stir for 1.5h to completely swell; wherein, the content of PVP in the mixture of chloroform and DMAc is fixed at 10 % (w / w), the content of PC in the mixture of chloroform and DMAc was fixed at 5% (w / w), and the mass ratio of ketoprofen to PC was 0:100, 3:25, 5:25, 10 :25, 15:25;

[0032] (3) Place the Erlenmeyer flask in a shaker and shake it at 25°C for 24 hours until it is completely dissolved. The polymer solution is transparent, and it is degassed by ultrasonic treatment for 15 minutes to obtain a spinning solution;

[0033] (4) Use a 5ml syringe (the inner diameter of the needle is 0.5mm) to extract the spinning solution, fix it on the electrospinning device, set the static voltage to 14kv, the recei...

Embodiment 2

[0037] (1) Take 10ml of liposome suspensions with different drug-to-lipid ratios, dilute to 25ml with methanol, and ultrasonically treat for 30 minutes to break the emulsion. Take 0.5ml from them and dilute to 100ml with double distilled water. Under the condition of a wavelength of 260nm, Measure its absorbance to calculate the total content of ketoprofen;

[0038] (2) Take 10ml of liposome suspensions with different drug-to-lipid ratios, put them in dialysis bags (MWCO: 3500), and seal them;

[0039] (3) At the same time, take 200ml of double-distilled water as the external dialysis fluid, stir at room temperature with a stirring speed of 150rpm for 24 hours, and measure the absorbance of the external dialysis fluid at a wavelength of 260nm to calculate the content of free ketoprofen;

[0040] (4) Calculate its encapsulation rate according to the following formula:

[0041] E.E.(%)=(1-free ketoprofen amount / total ketoprofen amount)×100, the ketoprofen encapsulation efficiency...

Embodiment 3

[0043] (1) Take 10ml of liposome suspension with a drug-to-lipid ratio of 5:25, put it in a dialysis bag (MWCO:3500), and seal it;

[0044] (2) At the same time, take 200ml of double-distilled water as the external fluid for dialysis, and shake at 37°C with a stirring speed of 150rpm for 24h to remove free ketoprofen;

[0045] (3) Replace the external dialysis fluid with buffer solutions of different pH values, keep the temperature at 37°C, shake at 150rpm, and take samples from it with a sampler at 0h, 0.5h, 1h, 2h, 4h, 6h, 12h, 24h, 48h and 72h 3ml (2 parallel operations), measure the absorbance at 260nm at each time point, and calculate the release percentage with the drug content in the 0h liposome as 100%. The release curves of ketoprofen from liposomes under different buffers are as follows: Figure 7 shown.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
diameteraaaaaaaaaa
Login to view more

Abstract

The invention relates to a method for preparing self-assembly ketoprofen liposome by an electrostatic spinning technology. The method comprises the following steps of: (1) adding polyvinylpyrrolidone, soybean lecithin and ketoprofen into a mixed solvent while stirring, continuously stirring for 1 to 2 hours until the mixed solvent is completely swelled, oscillating until the mixed solvent is completely dissolved, making solution transparent, and performing ultrasonic processing for degassing to obtain spinning solution; (2) performing electrostatic spinning by means of the spinning solution, and drying collected electrostatically-spun fibrous membranes in vacuum; and (3) dissolving the electrostatically-spun fibrous membranes in double distilled water to obtain liposome suspending liquid, performing ultrasonic processing, and thus obtaining the ketoprofen liposome. The method is easy to operate, has low time consumption and is suitable for large-scale production; the used raw materials are inexpensive and readily available; and the ketoprofen liposome has high degradability and biocompatibility and has the potential when applied to subsequent related experimental analysis.

Description

technical field [0001] The invention belongs to the field of preparation of drug liposomes, in particular to a method for preparing self-assembled ketoprofen liposomes by means of electrospinning technology. Background technique [0002] Molecular self-assembly is the use of molecular recognition between molecules or between one segment of a molecule and another segment to form molecular aggregates with a specific arrangement order through non-covalent interactions. In recent years, micro- and nano-materials prepared from biological building blocks have shown great application potential in materials science, tissue engineering, bioengineering, and drug delivery due to their good biocompatibility and structural and functional diversity. So far, a series of molecular self-assembly systems have been constructed, such as proteins, polypeptides and their derivatives, nucleic acids, and phospholipids. For a long time, many scientists have explored different methods to regulate it...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/127A61K31/192A61K47/32
Inventor 朱利民袁彩艳聂华丽
Owner DONGHUA UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap