99results about How to "High SERS activity" patented technology

The invention belongs to the technical fields of materials and biology, and in particular relates to a surface-enhanced Raman scattering (SERS) tag microsphere and a preparation method thereof. The preparation method comprises the following steps of: reducing silver nitrate solution by using a reducing agent by taking a polymer microsphere of which the surface is provided with a carboxyl functional group as a template by an in-situ chemical reduction method to prepare a composite microsphere with uniform and compact surface silver nanoparticle coverage degree; and cladding a silicon dioxide shell by using a chemical adsorption Raman probe molecule and a silane coupling agent by a sol-gel method to obtain the SERS tag microsphere with high SERS activity. The SERS tag microsphere has monodispersity, high stability and high biocompatibility, a silicon dioxide surface is easily modified into functional groups such as an amino group or carboxyl and the like, and the SERS tag microsphere is chemically combined with a biomolecule and is applied to the fields of immunoassay, biomedical imaging, disease treatment and the like. The method is easy to operate, and has a controllable process and a good application prospect.

The invention discloses a silicon nano cone array coated with a gold film as well as a preparation method and an application thereof. The array is a silicon nano cone sequence array with a surface coated with a gold film, wherein the conical bottom diameter of silicon nano cones forming the silicon nano cone sequence array is 180nm to 220nm, the cone height is 450nm to 550nm, the cone period is 250nm to 350nm, and the thickness of the gold film is 15nm to 25nm. The preparation method comprises the following steps: first synthesizing polystyrene colloidal spheres on a silicon substrate to form a single-layer colloidal crystal template by virtue of a gas-liquid interface self-assembling technology, placing the silicon substrate with the single-layer colloidal crystal template in a sulfur hexafluoride atmosphere, etching the silicon substrate by virtue of plasma to obtain the silicon substrate with silicon nano cone sequence array, and depositing the gold film on the silicon substrate with the silicon nano cone sequence array by utilizing an ion beam sputtering technology or thermal evaporation deposition technology to obtain a target product. The silicon nano cone array can be used as an active substrate of surface enhanced raman scattering to measure the content of clenbuterol hydrochloride attached thereon.

The invention discloses a gold nanoparticle-silver nano-semisphere array as well as a preparation method and the application of the array. The array is an ordered array which is placed on a silver membrane of a substrate and attached with silver nano-semispheres, wherein the sphere diameter of the silver nano-semisphere is 85-95 nm; the sphere interval is less than or equal to 10 nm; the gold nanoparticles are modified on the silver nano-semispheres; the particle size of the gold nanoparticle is 5-10 nm. The preparation method comprises the following steps: sequentially performing secondary anodic oxidation, reaming treatment and silver membrane plating on one side of an aluminum sheet, so as to obtain an aluminum oxide template covered with the silver membrane on one side and collected with the silver nano-semispheres in the hole; adhering and fixing the substrate to the silver membrane; then, placing the aluminum oxide template covered with the silver membrane and the substrate on one side and collected with the silver nano-semispheres in the hole in an alkali solution to etch off the aluminum oxide template, and then putting the rest in an ion sputtering device to perform gold sputtering for 8-12 s under the sputtering current is 35-45 mA, so as to obtain the objective product. The array can be taken as an active substrate for enhancing raman scattering on the surface, so that the content of trace rhodamine or polychlorinated biphenyl 3 attached on the array can be measured by a laser raman spectrometer.

The invention discloses a film formed by silver nanosheets and a preparation method and application of the film. The film is 200-500nm in thickness and is formed by covering a nanometer flat branch cluster on a conductive substrate, wherein a gap between every two nanometer flat branches in the cluster is 1-15nm; and the film is formed by connecting nanosheets with arc-shaped edges in series, wherein the thickness of each nanosheet is 10-50nm, width is 30-300nm and length is 90-150nm. The preparation method comprises the following steps of: mixing and stirring a silver nitrate water solution, a citric acid water solution and water to obtain a mixed solution, and injecting a sodium borohydride water solution into the mixed solution and stirring and aging to obtain a silver seed crystal solution; then coating the silver seed crystal solution onto the conductive substrate and then airing the conductive substrate; and then putting the conductive substrate which is coated with silver seed crystals and is used as a cathode in electrolyte consisting of the silver nitrate water solution and the citric acid water solution, and carrying out electro-deposition for 5-120 minutes under the constant current of which the current density is 0.1-1mA/cm<2> to obtain the target product. The film disclosed by the invention can be used as an SERS (Surface Enhanced Raman Scattering) active substrate to rapidly detect the content of trace rhodamine 6G or tetrachlorobiphenyl-77 attached onto the SERS active substrate.

The invention discloses a hollow core-shell nanoparticle and a preparation method, a test strip and a test method. The concentration of determinand in a sample solution can be quantitatively detected,and the detection sensitivity can be improved. The hollow core-shell nanoparticle includes a core body and a shell body, the shell body is located on the outer side of the core body, there is a spacebetween the shell body and the core body, the outer surface and the inner surface of the shell body are marked with Raman molecules respectively, and the outer surface of the core body is marked withthe Raman molecules; the shell body is made of a gold-silver alloy, and the core body is made of gold.

The invention discloses a silver nanoparticle modified carbon nanotube-egg membrane composite surface enhanced Raman scattering (SERS) substrate as well as a preparation method and application thereof. The SERS substrate is composed of a carbon nanotube loaded on an egg membrane and silver nanoparticles modified on the carbon nanotube, wherein the carbon nanotube is 0.5-2mu m long and is 30-50nm in diameter, and sizes of the silver nanoparticles are 5-10nm. The preparation method comprises the following steps: dissolving egg shell in an acetic acid solution to obtain an egg membrane, ultrasonically processing the egg membrane in an ammonium solution to obtain alkalified egg membrane, then dispersing a carbon nanotube processed by carboxylation in de-ionized water and ultrasonically processing to obtain a carbon nanotube solution, and ultrasonically processing the alkalified egg membrane in the carbon nanotube solution to obtain the egg membrane loaded with the carbon nanotube on the surface; and finally, sputtering silver on the egg membrane in a sputtering instrument to obtain a target product. The SERS substrate disclosed by the invention can serve as an active substrate for surface enhanced Raman scattering; and through a laser Raman spectrometer, content of rhodamine or parathion-methyl or polychlorinated biphenyl PCB-3 or bovine serum albumin attached on the substrate is measured.

The invention discloses a silver nanoparticles-assembled monolayer inverse opal structure, and a preparation method and a use thereof. The structure is a rod-shaped substance composed of an alumina hemispherical shell, a gold film and silver nanoparticls which are sequentially coated on a substrate, wherein the substrate is a conductive substrate, the diameter of the alumina hemispherical shell is 100 nm to 10 [mu]m, the thickness of the spherical shell is 50-500 nm, the thickness of the gold film is more than or equal to 5 nm, the thickness of the gold nanoparticles is 10-30 nm, and the rod-shaped substance is a hemispherical-bottomed strip formed by serially connecting the silver nanoparticles and a hemispherical-shelled rod formed by accumulating the silver nanoparticles and having two tapered ends. The method comprises the following steps: synthesizing a single layer of colloidal crystals on the substrate, filling gaps among the colloidal spheres with an aqueous solution of aluminum nitrate, using a chemical or physical technology to remove the monolayer colloidal crystal template, annealing the obtained material, coating the annealed material with the gold film, and carrying out electrodeposition in a silver electrolyte by using the coated material as a cathode and a graphite sheet as an anode to prepare the target product. The product has a stable structure, and can be easily and commercially widely applied to the rapid detection of a tiny amount of pesticide residues in vegetables.

The invention provides a method for preparing a surface-enhanced Raman scattering (SERS) substrate. The method utilizes a melanin membrane as a key substrate surface morphology control layer on the SERS substrate and utilizes metal nanoparticles prepared from a melanin reduction metal salt solution to prepare the SERS substrate. Through reduction effects of a melanin organic molecule functional group, a metal ion is directly reduced into a metal nanoparticle and the metal nanoparticle is deposited on the surface of the melanin film, and through control of an effective area and density of the organic molecule functional group and reaction conditions of metal ion reduction into an atom and polymerization of atoms into a nanometer cluster, metal nanoparticle sizes and distance are adjusted and controlled so that metal nanoparticle/melanin film SERS performances are optimized. The method is used for detection of trace quantity of substances. A detected sample is directly put on the metal nanoparticle-loaded melanin film so that detection is finished. The method is free of a reduction additive in metal nanoparticle reduction, has good response enhancement effects on SERS detection and is not influenced by a background interference element.

The invention relates to a composite nanomaterial of a core-shell structure, a preparation method thereof and application thereof. The composite nanomaterial has a core-shell structure, and comprises silver nanoparticles serving as inner cores and gold and/or platinum layers serving as outer shells, the surfaces of the silver nanoparticles carry first raman detection molecules, the gold and/or platinum layers wrap the surfaces of the silver nanoparticles, and the outer surfaces, away from the silver nanoparticles, of the gold and/or platinum layers carry second raman detection molecules. The invention further discloses the preparation method and application of the composite nanomaterial. The composite nanomaterial is applied to the diagnosis and treatment process of tumors, and a novel multifunctional molecular probe with low toxicity, high stability, high SERS activity and a photo-thermal treatment effect can be obtained.

The invention discloses an SERS (surface enhanced Raman scattering) device, as well as a preparing method and application thereof. The device comprises a capillary tube with a mixing colloid and an active substrate both arranged therein, wherein the mixing colloid consists of poly-(N-isopropyl acrylamide), gold nanorods and water with the weight ratio of (1.8-2.2):(0.001-0.003):(100); the active substrate adopts the structure that tapered zinc oxide nanorods with silver nanoparticles modified surfaces are stood on the surface of a heating wire. The method includes the following steps: adding ammonia water in a zinc nitrate hexahydrate solution, and obtaining an electric precipitating solution after the ammonia water is completely dissolved; using the heating wire as a cathode and placing the heating wire in the solution for electric precipitation, so as to obtain the heating wire with tapered nanorods stood on the surface; then immersing the heating wire with the tapered nanorods stood on the surface in a silver nitrate solution and irradiating the heating wire by ultraviolet light, so as to obtain an active substrate; then placing the active substrate and the mixing colloid in the capillary tube, so as to prepare the target product. According to the invention, the SERS device can be widely applied in real-time detection of pollutants in water solutions in environmental, chemical, biological fields and the like.

The invention provides a preparation method of an SERS (Surface-Enhanced Raman Scattering) chip. By an ultrasonic spray coating method, a dispersion solution containing metal nanometer particles is sprayed and coated onto the surface of a substrate with a plurality of concave pits on the surface; solvents of the dispersion solution are removed through volatilization, so that the metal nanometer particles are self-assembled in the concave pits of the substrate. The ultrasonic spray coating is used for preparing the SERS chip; the cost is low; the high-quality SERS chip can be prepared at high flux; the SERS chip prepared by the method has good SERS activity, ultrahigh uniformity, batch reproducibility and ultrahigh stability, and can be used for trace substance detection.

The invention belongs to the field of material chemistry and detection and relates to a performance-controllable layer-by-layer assembled nanoparticle SERS substrate and a preparation method thereof. The substrate comprises a carrier, the surface of the carrier is provided with a polar group, the surface of the carrier is coated with a layer-by-layer assembled film, from inside to outside, even number layers of the layer-by-layer assembled film are negatively charged metal nanoparticle films, odd number layers of the layer-by-layer assembled film are positively charged polyelectrolyte films and the outermost layer is a negatively charged metal nanoparticle film. Through loading with metal nanoparticle films with the same metal and different particle sizes, SERS substrate performance regulation and control are realized. The preparation method is simple and effective and has wide applicability. The SERS substrate has good SERS activity and can be used for micromolecule detection.

The invention belongs to the technical field of analysis and detection, and particularly discloses to a fluorescent-Raman dual probe material for detecting zinc ions and a preparation method thereof.The preparation method comprises the following steps: (1) firstly, performing a reaction on N-tert-butoxycarbonylethylenediamine and chloromethylpyridine to produce an N-Boc-protected dipyridine compound, and then removing a Boc protecting group by hydrofluoric acid to obtain N, N-dimethylpyridineethylenediamine; (2), performing an amidation reaction on the N, N-dimethylpyridineethylenediamine andmercaptoacetic acid to obtain bipyridinemercaptoacetamide; (3), with a self-assembly method, modifying the bipyridinemercaptoacetamide onto the surfaces of gold nanoparticles, incubating at room temperature, and centrifuging to obtain the fluorescent-Raman dual probe material for detecting the zinc ions. The preparation method is simple and easy to implement; the obtained fluorescent-Raman dual probe material can specifically recognize the zinc ions, and is high in sensitivity and fast in response; the fluorescent-Raman dual probe material has a potential application prospect in the fields ofbioimaging, environment and the like.

The invention discloses a silver-germanium-copper composite structural component and a preparation method and use thereof. The silver-germanium-copper composite structural component is prepared by constructing of germanium nanowires modified with silver nanoparticles on the surface and the mesh wall of a copper mesh, the mesh diameter of the copper mesh is 35-45 mum, the germanium nanowire line diameter is 100-150 nm, the line length is 5-15 mum, the silver nanoparticle grain diameter is 15-35 nm; and the method is as follows: the copper mesh is put at a position 1.5-2.5 cm from a gold target of a sputtering device for sputtering in the sputtering current of 15-25 mA for 1.5-2.5 min to obtain the copper mesh on which the surface and the mesh wall are evaporated with gold nanoparticles, then the germanium nanowires are grown on the copper mesh by chemical gas phase method to obtain the copper mesh on which the surface and the mesh wall are constructed with the germanium nanowires, and the sputtering device is used for sputtering the silver nanoparticles on the copper mesh on which the surface and the mesh wall are constructed with the germanium nanowires to obtain an objective product. The silver-germanium-copper composite structural component can be used as surface enhanced Raman scattering active base, and can be widely used for measuring the content of rhodamine 6G, or methyl parathion-methyl, or adenine, or 6-aminopenicillanicacid or alkanes acid or penicillin G sodium salt attached on the silver-germanium-copper composite structural component.

The invention discloses a preparation method and an application of a flexible transparent SERS substrate. The method comprises the following steps of heating an HAuCl4 water solution to be boiling under vigorous stirring, rapidly adding a citrate aqueous solution, and condensing and refluxing for 13-17 minutes after the color change of the solution is stable, stopping heating, and cooling to roomtemperature under stirring to obtain Au nano-sol; and adding the Au nano-sol into a beaker, and slowly injecting a mixed solution of n-hexane and ethanol into the Au nano-sol by using a needle cylinder syringe at room temperature, slowly forming two phases of water oil, wherein a layer of nanometer Au film is gradually formed at the water-oil interface, and after the oil phase naturally volatilizes, directly covering by using an adhesive tape for uncovering the single-layer nano Au on the water phase surface, removing residual gold sol on the surface of the adhesive tape by using deionized water to prepare the flexible transparent SERS substrate. By adoption of the technology, the Au nano-particles can have better distribution uniformity on an adhesive tape, the flexibility and the viscosity of the adhesive tape can be well maintained, direct measurement can be carried out by a laser backlight mode, and in-situ detection is realized.

The invention discloses a silver-germanium-silicon heterogeneous hierarchical structure array, and a preparation method and an application of the array. The array is an array of silicon micron hexagonal prisms in hexagonal arrangement, wherein the silicon micron hexagonal prisms forming the array of silicon micron hexagonal prisms in hexagonal arrangement are provided with germanium nanocones, surfaces of which is decorated with silver nanoparticles. The preparation method comprises: mixing a nickel nitrate solution and an oxidized grapheme solution to obtain a mixed liquid; immersing the array of silicon micron hexagonal prisms in hexagonal arrangement obtained via photoetching and deep silicon etching technique in the mixed liquid to obtain the array of silicon micron hexagonal prisms, surfaces of which are decorated with nickel nitrate, in hexagonal arrangement; then applying a chemical vapor deposition method to enable the array of silicon micron hexagonal prisms, surfaces of which are decorated with nickel nitrate, in hexagonal arrangement to be deposited with the germanium nanocones; and immersing the array of silicon micron hexagonal prisms, surfaces of which are decorated with the germanium nanocones, in a silver nitrate solution to obtain the target product. The silver-germanium-silicon heterogeneous hierarchical structure array can be used as an active matrix of SERS, and is widely applicable to fast detection of the fields, such as environment, chemistry, and biology.

The invention discloses a compound type multilayer film structure silver nanowire and a preparation method thereof. The method of combining the physical deposition technology and annealing treatment is mainly adopted, a [Ag 20-30nm/SiO2 3-5nm]n nano-multilayer film array is prepared on a two-dimensional ordered colloidal sphere mold through the magnetron sputtering technology and further subjected to annealing treatment under the protection of argon, and thus the silver nanowire grows on the surface of a compound type multilayer film structure. According to the method, preparation cost is low, and consumed time is short. The method has the characteristics that the structure is stable, the controllability is high and the purity is high. Silicon dioxide and silver are combined together, the silicon dioxide can change the dielectric environment around metal and can protect the silver against oxidization, and thus the survival time of the silver nanowire structure is longer. In addition, the structure has abundant hot spots and high surface enhanced Raman scattering (SERS) activity and can be applied to the fields of molecular detection, recognition technology, electronics, magnetic, acoustics, optics and the like.

The invention discloses a germanium nanotube top raised array modified by silver nano-particles as well as a preparation method and application thereof. The array is formed by modifying a raised surface of a germanium nanotube in an aluminium oxide template and a template therein with silver nano-particles. The preparation method comprises the following steps: firstly soaking a blind-hole aluminum oxide template in a mixed aqueous solution of nickel nitrate and phosphoric acid, and then soaking and washing the blind-hole aluminum oxide template with a nickel nitrate aqueous solution, thereby obtaining the blind-hole aluminum oxide template with nickel nitrate absorbed on a hole wall; depositing a germanium nanotube in a blind hole by utilizing a chemical vapor deposition method through the blind-hole aluminum oxide template with nickel nitrate absorbed on the hole wall, and soaking the aluminum oxide template in a sodium hydroxide aqueous solution after removing the un-oxidized aluminum on the back surface of the template by utilizing a stannic chloride solution, thereby obtaining the aluminum oxide template with the germanium nanotube with raised and exposed top in the blind hole; sputtering the silver nano-particles onto the aluminum oxide template in a plasma sputtering instrument, thereby obtaining a target product. The germanium nanotube top raised array can be used as an active substrate of SERS (surface enhanced raman scattering) and can be widely used for the rapid detection in the fields of environment, chemistry, biology and the like.

The invention discloses a gold nanotube array with gold and sliver nanowires built on the surface and a preparation method and application thereof. The gold and sliver nanowires growing on the radial direction of each tube are built on the inner wall and the outer wall of each gold nanotube of the array, wherein the external diameter of each gold nanotube is 60-80 nm, the lengths of the gold and sliver nanowires are 6-10 nm, and the diameters of the wires are 3-6 nm. The method comprises the following steps: using an aluminum sheet to manufacture a through-hole alumina template with a two-step anodic oxidation method, carrying out evaporation deposition of gold film on one surface of the through-hole alumina template, then putting the through-hole alumina template into gold electrolyte for electro-deposition, and thus obtaining the alumina template which is coated with the gold film on one surface and provided with gold nanotubes in holes; removing the alumina template in an acid or alkali solution, and putting the gold nanotube array in mixed liquid of aniline and lauryl sodium sulfate for ultrasonic reaction; sequentially adding a chloroauric acid solution and a silver nitrate solution drop by drop in the mixed liquid subjected to the ultrasonic reaction, leaving to stand at the room temperature, and thus obtaining the target product. The target product can be used as an SERS substrate and is used for tract detection of organic pollutants.