Hollow core-shell nanoparticle and preparation method, test strip and test method

Abstract

The invention discloses a hollow core-shell nanoparticle and a preparation method, a test strip and a test method. The concentration of determinand in a sample solution can be quantitatively detected,and the detection sensitivity can be improved. The hollow core-shell nanoparticle includes a core body and a shell body, the shell body is located on the outer side of the core body, there is a spacebetween the shell body and the core body, the outer surface and the inner surface of the shell body are marked with Raman molecules respectively, and the outer surface of the core body is marked withthe Raman molecules; the shell body is made of a gold-silver alloy, and the core body is made of gold.

Description

technical field [0001] The invention relates to the field of biomedical testing, in particular to a hollow core-shell nanoparticle and a preparation method, a test strip and a testing method. Background technique [0002] The immunochromatographic detection technology based on gold nanoparticles (Au NPs) is a membrane-based in vitro detection and diagnosis method developed on the basis of monoclonal antibody technology and new nanomaterial technology. It has been widely used in many fields, including allergies, Early pregnancy, myocardial infarction markers, infectious diseases, environmental pollutants, drugs, etc. Taking early pregnancy detection as an example, the amount of kits / test strips required each year has reached 10 7 indivual. The principle of immunochromatographic detection based on Au NPs is as follows: the specific capture antibody is immobilized on the nitrocellulose (NC) membrane to form bands; the AuNPs complex bound to the recognition antibody is loaded ...

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Problems solved by technology

However, the magnetic-based immunochromatography method is easily interfered by

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