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Surface-enhanced Raman scattering (SERS) tag microsphere and preparation method thereof

A microsphere and label technology, applied in the field of SERS-labeled microspheres and their preparation, can solve the problems of reduced SERS activity, time-consuming, complicated preparation process, etc., and achieve the effects of high SERS activity, high biocompatibility, and simple preparation process.

Inactive Publication Date: 2011-10-05
FUDAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the above methods can obtain substrates with high SERS activity, these methods require multiple steps, and the preparation process is cumbersome and time-consuming; moreover, the silver nanoparticles and Raman probe molecules are exposed outside and are easily affected by the external environment, resulting in reduced SERS activity and The signal is susceptible to interference from external molecules

Method used

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  • Surface-enhanced Raman scattering (SERS) tag microsphere and preparation method thereof
  • Surface-enhanced Raman scattering (SERS) tag microsphere and preparation method thereof
  • Surface-enhanced Raman scattering (SERS) tag microsphere and preparation method thereof

Examples

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Effect test

Embodiment 1

[0023] Example 1: Preparation of a SERS label using 4-aminothiophenol (4-ABT) as a Raman probe molecule

[0024] 1. Preparation of polymer template (PSA)

[0025] Add 2.0 g of styrene, 0.1 g of acrylic acid and 100 ml of deionized water into a 250 ml three-necked flask equipped with a mechanical stirrer and a condenser, and ventilate nitrogen for 30 minutes to remove oxygen in the flask; then raise the temperature to 70°C, and add 0.08 g potassium persulfate aqueous solution (dissolved in 2ml water in advance), after heat preservation for 10h, centrifuge and wash with deionized water to remove unreacted monomer and initiator, then disperse in deionized water to prepare microsphere content of 0.5wt% lotion.

[0026] 2. Preparation of polymer / silver nanoparticles composite microspheres (PSA@AgNPs)

[0027] Add 2ml of the polymer microspheres obtained above into a 100ml single-necked flask, then add 20ml of 0.1mol / L silver nitrate aqueous solution, and stir magnetically at room...

Embodiment 2

[0030]Example 2: Preparation of a SERS label using 4-hydroxybenzenethiol (4-HBT) as a Raman probe molecule

[0031] 1. The preparation of the polymer template (PSA) is the same as described in Example 1-1.

[0032] 2. The preparation of polymer / silver nanoparticle composite microspheres (PSA@AgNPs) is the same as described in Examples 1-2.

[0033] 3. SERS-labeled microspheres (PSA@AgNPs / 4-HBT@SiO 2 ) was prepared as described in Examples 1-3. The difference is that the Raman labeling molecule is 4-hydroxybenzenethiol (4-HBT), and the SERS spectrum of the final SERS label is as follows Figure 4 shown.

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Abstract

The invention belongs to the technical fields of materials and biology, and in particular relates to a surface-enhanced Raman scattering (SERS) tag microsphere and a preparation method thereof. The preparation method comprises the following steps of: reducing silver nitrate solution by using a reducing agent by taking a polymer microsphere of which the surface is provided with a carboxyl functional group as a template by an in-situ chemical reduction method to prepare a composite microsphere with uniform and compact surface silver nanoparticle coverage degree; and cladding a silicon dioxide shell by using a chemical adsorption Raman probe molecule and a silane coupling agent by a sol-gel method to obtain the SERS tag microsphere with high SERS activity. The SERS tag microsphere has monodispersity, high stability and high biocompatibility, a silicon dioxide surface is easily modified into functional groups such as an amino group or carboxyl and the like, and the SERS tag microsphere is chemically combined with a biomolecule and is applied to the fields of immunoassay, biomedical imaging, disease treatment and the like. The method is easy to operate, and has a controllable process and a good application prospect.

Description

technical field [0001] The invention belongs to the field of materials and biotechnology, and in particular relates to a SERS label microsphere and a preparation method thereof. technical background [0002] Raman spectroscopy is a kind of vibrational spectroscopy, which has many advantages when applied to detection and analysis: (1) It can provide information on chemical structures and functional groups at the molecular level and can be used to identify specific biomolecules; (2) The spectral peak width is narrow, and the signal (3) It is not interfered by water, oxygen, etc., and can detect aqueous samples; (4) It does not destroy the structure of the sample, and can provide non-destructive testing, especially suitable for the detection of biological samples. Therefore, Raman spectroscopy has received extensive attention in the fields of molecular imaging of biological samples and clinical diagnosis. However, the signal intensity of Raman spectroscopy is very low, which g...

Claims

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Application Information

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IPC IPC(8): C08J7/12C08L25/08C08F212/08C08F220/06C08F2/22G01N21/65
Inventor 汪长春李菊梅
Owner FUDAN UNIV
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