The invention discloses a liquid-based cell detection test kit for cervical cancer screening and a use method, and the main points of the technical scheme are as follows: the kit comprises a liquid-based cell cleaning solution and a cell staining solution, and the liquid-based cell cleaning solution comprises a cell preserving solution and sucrose; the cell preserving solution is prepared from thefollowing components: paraformaldehyde, a phosphate buffer solution, ascorbic acid, EDTA-Na2.2H2O, N-acetyl-L-cysteine, 4M NaOH, tween-20 and sterile water, the cell staining solution comprises hematoxylin and an EA50 staining solution, and the hematoxylin comprises the following components: aluminum potassium sulfate, hematoxylin, sodium iodate, acetic acid, absolute ethyl alcohol, glycerol andsterile water; the EA50 staining solution comprises the following components: bright green SF, eosin, methanol, phosphotungstic acid, lithium carbonate, glacial acetic acid and sterile water. The liquid-based cell detection test has the advantages of high cell preservation stability, good cell fixation, preservation of complete cell morphology, prevention of mucus interference and cell aggregation, effective red blood cell lysis and the like; the liquid-based cell detection test is low in price, convenient and easy to obtain, and the liquid-based cell preserving solution low in cost and convenient to popularize and apply.