Liquid-based cell detection kit for cervical cancer screening and use method

Abstract

The invention discloses a liquid-based cell detection test kit for cervical cancer screening and a use method, and the main points of the technical scheme are as follows: the kit comprises a liquid-based cell cleaning solution and a cell staining solution, and the liquid-based cell cleaning solution comprises a cell preserving solution and sucrose; the cell preserving solution is prepared from thefollowing components: paraformaldehyde, a phosphate buffer solution, ascorbic acid, EDTA-Na2.2H2O, N-acetyl-L-cysteine, 4M NaOH, tween-20 and sterile water, the cell staining solution comprises hematoxylin and an EA50 staining solution, and the hematoxylin comprises the following components: aluminum potassium sulfate, hematoxylin, sodium iodate, acetic acid, absolute ethyl alcohol, glycerol andsterile water; the EA50 staining solution comprises the following components: bright green SF, eosin, methanol, phosphotungstic acid, lithium carbonate, glacial acetic acid and sterile water. The liquid-based cell detection test has the advantages of high cell preservation stability, good cell fixation, preservation of complete cell morphology, prevention of mucus interference and cell aggregation, effective red blood cell lysis and the like; the liquid-based cell detection test is low in price, convenient and easy to obtain, and the liquid-based cell preserving solution low in cost and convenient to popularize and apply.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a liquid-based cell detection kit for cervical cancer screening and a use method. Background technique [0002] Cervical cancer is one of the most common malignant tumors in the female reproductive system. Cervical cancer is the only cancer with a clear cause of all cancers—persistent infection with high-risk HPV. Cervical cancer is the second most common cancer among women worldwide, with approximately 500,000 new cases each year. In the past 20 years, the incidence of cervical cancer has gradually become younger, and the incidence of cervical cancer in women has been increasing year by year. Cervical cancer has become an important disease that threatens women's health and has attracted the attention of the World Health Organization. In developing countries, the incidence and mortality of cervical cancer continue to increase, and more than 80% of cervical cancers occur in developi...

AI Technical Summary

Problems solved by technology

Liquid-based thin-layer cytology detection technology has a positive detection rate of more than 95% for cervical cancer cells. At the same time, it can also detect precancerous lesions and microbial infections such as mold, trichomonas, and chlamydia, which solves the false negative of traditional Pap smears High rate, high rate of lost cells (80%) and poor quality of smears and other technical difficulties

[0005] At present, the liquid-based cell fluid produced by Thinprere of the United States is commonly used in the market. When this product is used in conjunction with the company's Thinprere-2000 instrument, the detection rate of cervical squamous epithelial neoplasia has increased by more than 100%, and it can make Minimize the error rate of unsatisfactory specimens and uncertain cytology results, which can significantly improve the early detection rate of cervical lesions, but because of its high price, it is only promoted in some large hospitals, and it is difficult to promote it to low-income areas or grassroots , cannot be popularized

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