40 results about "Cell fixation" patented technology

The invention belongs to the technical field of biology, and discloses a stable protective agent, a cell antigen dry sheet and a preparation method and application of the dry sheet, the stable protective agent comprises glycerin with the volume fraction being 0.4%, gelatin with the mass volume fraction being 0.1%, EDTA with the mass volume fraction being 0.1 mM, trehalose with the mass volume fraction being 0.03% and resveratrol with the mass volume fraction being 100 [mu] M. The stable protective agent is adopted for preparing the cell antigen dry sheet, and the cell antigen dry sheet is applied to CBA detection. The stable protective agent has good permeability, can penetrate through cell membranes to enter cells, provides a protection effect inside and outside the cells, has moisture retention and oxidation resistance, can prevent cell deformation and cell fixing layer shedding, and can well maintain the stability and reliability of antigen proteins, and the prepared cell antigen dry sheet can be stored for a long time, and has a good application prospect. The antigenicity of a membrane signal index is not damaged. When a positive sample is detected, obvious green fluorescence cells can be seen, red fluorescence quenching is avoided, and co-localization of red fluorescence and green fluorescence can be observed conveniently.

According to the cell fixing liquid, phenol oxidase is used for catalyzing phenol to be oxidized into a quinone structure, the quinone structure reacts with protein amido to form light covalent immobilization, cell immobilization is achieved, a stable hydration environment and membrane protection are provided through the ionic liquid solvent choline dihydrogen phosphate, a phosphate-free buffer system is provided, and cell immobilization is achieved. Therefore, a novel cell fixing liquid is obtained, the cell structure integrity required by cell imaging analysis is effectively reserved, meanwhile, cell staining fluorescence signal interference is avoided, and therefore the accuracy of cell counting and cell imaging analysis is ensured.

The present invention relates to reagent-coated cover slips and methods of using them to carry out one or more reactions. The present invention provides methods using reagent-coated cover slips to carry out a test on a carrier slide. The present invention provides methods where sample cells are immobilized on a carrier slide and a test is carried on the carrier slide. The present methods carry out one or more chemical reactions in the embedded sample cells on a carrier slide by contacting reagent-coated cover slips with sample cells immobilized on the carrier slide to result in a reaction product on the carrier slide.

The application provides a cell fixing solution for a myasthenia gravis antibody spectrum (IgG) detection kit (cellular immunofluorescence method) and a product and application thereof, and relates to the technical field of in-vitro detection. The cell fixing solution provided by the application is composed of polyoxymethylene, formaldehyde, acetic acid, raffinose and a buffer solution, can effectively fix the cell morphology, maximally retains the natural spatial conformation and antigen epitope integrity of myasthenia gravis related antigens AChR, Titin, RyR1, MuSK and LRP4, reduces the problems of antigen denaturation, epitope shielding and protein loss in the fixing process, can efficiently and accurately realize the synchronous joint detection of various specific antibodies, and the myasthenia gravis antibody spectrum (IgG) detection kit (cellular immunofluorescence method) prepared by the application has stable detection results, and has important clinical application value and industrial popularization prospect.

This invention belongs to the field of biotechnology and discloses a dry film stabilizer, cytoplasmic antigen dry films, their preparation methods, and applications. The dry film stabilizer includes polyethylene glycol, trehalose, BSA, melatonin, and Tween-20. Cytoplasmic antigen dry films are prepared using this stabilizer and applied in CBA detection. This dry film stabilizer has good permeability, penetrating the cell membrane to enter the cell interior, providing protection both inside and outside the cell. It also possesses moisturizing and antioxidant properties, preventing cell deformation and shedding of the cell fixation layer, effectively maintaining the stability and reliability of intracellular antigen proteins. The prepared cytoplasmic antigen dry films can be stored long-term without damaging the antigenicity of cytoplasmic signal indicators. When detecting positive samples, strong and numerous green filamentous fluorescence signals are observed in the cytoplasm, with consistent green and red fluorescence intensities. Software synthesis reveals co-localization of green and red antigen fluorescence.

The invention relates to the technical field of liquid-based cytology, and particularly discloses serosa effusion cell preserving fluid. Comprising a preservation solution, an anticoagulant, a red blood cell treatment solution, a cell membrane stabilizer, an antioxidant and a metal ion chelating agent, the preservation solution contains a buffer system, an energy supply component, an antibiotic and a surfactant, and the red blood cell treatment solution contains a hemolysis active component and a cell immobilization component; the preparation method comprises the following steps: firstly, preparing the preserving fluid, adjusting the pH value, adding the active substances, mixing the antibiotics, and then completing constant volume and sterile filtration; the anticoagulant solution and the red blood cell treating fluid are independently prepared, and sterilization is achieved through filtration; and finally, packaging all the components in a sterilized storage bottle in a matched manner. The composition provided by the invention can be used for preserving cells in a clinical serosal effusion sample, and can provide a high-quality sample for subsequent cell morphology observation or detection; in addition, according to the preparation method, the controllability is high when all the components are independently prepared, and the preserving fluid with the consistent performance can be stably prepared.

The embodiments of the present application provide a battery assembly, a battery module and a battery energy storage device. The battery assembly 10 comprises: a heat insulation fixing frame 101, a top isolation plate 102 and at least one cell 103; the heat insulation fixing frame 101 surrounds the at least one cell 103 to fix the position of the at least one cell 103; the cell 103 comprises a cell main body 1031, an electrode 1032 and a cell pressure relief valve 1033, the cell pressure relief valve 1033 being used for releasing flue gas; the top isolation plate 102 is mounted on the top of the heat insulation fixing frame 101 and is attached to the top of the heat insulation fixing frame 101; at least one first flue gas channel 1021 is provided on the top isolation plate 102; the first flue gas channel 1021 is used for discharging flue gas; the position of the first flue gas channel 1021 is opposite to the position of the cell pressure relief valve 1033; and the top isolation plate 102 is provided with at least one first electrode through hole 1022, so that the electrode 1032 is electrically connected through the first electrode through hole 1022. Therefore, the potential safety hazard during thermal runaway of the cell is reduced.

The invention provides a structured cell growth matrix or assembly comprising a one or more spacer layers and one or more cell immobilization layers. The invention further provides a bioreactor comprising said matrix or assembly.

The invention discloses an immunofluorescence staining method for karyocytes in blood. When immunofluorescence staining is carried out, polylysine is used for treating a cell slide, and then the cell is fixed and smeared and dried, so that the cell and the slide are attached more tightly. The problem that cells fall off in the cleaning process can be effectively avoided. Meanwhile, non-blood cells in the blood sample can be effectively reserved.

A rapid detection method for cytotoxic T cells in peripheral blood. The method enables rapid identification of cytotoxic T cells in peripheral blood by detecting the expression of T-cell surface protein CX3CR1, or CX3CR1 and GPR56. The detection method is rapid in operation, does not involve cell fixation and permeabilization, and does not affect cell activity.

This invention discloses a detection kit and identification method for CTCs based on SCLC molecular typing using microfluidic chips and multiplex immunofluorescence probe technology. The kit includes a microfluidic chip, a biotin-labeled trapping agent, a balanced salt solution, a tissue cell fixation solution, a specific hybridization blocking solution, an active nuclear staining solution, and a diluent. It also includes a detection agent composed of key subtype markers of SCLC labeled with different fluorescein groups, SCLC-related marker detection antibodies, and leukocyte auxiliary identification markers, or a detection agent composed of key subtype markers of SCLC labeled with different fluorescein groups and leukocyte auxiliary identification markers. This application enables non-invasive, early, and sensitive detection and analysis of the expression of four immunomarkers involved in CTCs in the peripheral blood of patients: ASCL1, NEUROD1, POU2F3, and YAP1. It is suitable for early tumor screening and can also be used for pan-cancer immunotherapy guidance, immunotherapy efficacy monitoring, and prognostic assessment.

The utility model relates to a potting energy storage battery pack, and aims to solve the problems of unstable fixation of battery cells, welding defects and high customization cost in the prior art. The battery pack comprises a box body, a battery module, a battery bracket and a welding bracket, the battery module consists of a plurality of cylindrical battery cells and a busbar, and the battery cells are connected with the busbar through welding surfaces; the battery bracket is provided with at least two groove surfaces and is used for fixing the battery cells, and the adjacent battery cells are connected through the groove surfaces; the welding support is of a groove-shaped structure, the bottom of the welding support is provided with a first positioning hole and a welding platform, and a busbar of a battery cell is accurately welded through the welding platform. According to the utility model, the busbar welding mode of the battery cell is optimized, the instability caused by assembly tolerance is reduced, and the high consistency of a welding surface is ensured, so that the problem of insufficient welding is effectively avoided, and the welding quality and the overall safety of a battery pack are improved.

The invention belongs to the field of super-resolution imaging, and particularly discloses a preparation method of a DNA probe and application of the DNA probe in super-resolution imaging of cell and tissue slice DNA, rhodamine dye modified by different groups is connected with a Hoechst structure through a short connecting chain, and the self-flickering DNA probe is formed; the spiro opening and closing capability of the fluorescent dye in the probe is modified and adjusted through different groups, so that fine regulation and control on the performance of the DNA probe are realized, and different SMLM super-resolution imaging requirements are met; sMLM imaging of DNA in living cells, fixed cells, tissue frozen sections and clinical tissue paraffin sections is achieved by combining the SMLM technology with a self-scintillation probe with DNA specificity, and the resolution ratio is far higher than that of a traditional imaging technology.

A method for measuring organelle temperature, according to the present invention, may comprise the steps of: binding antibodies to a fluorescent nanodiamond so as to prepare an antibody-fluorescent nanodiamond; culturing cells with the antibody-fluorescent nanodiamond so as to prepare a sample; preparing a first source in which a cell fixation solution is provided to the sample, and a second source in which an ATP synthesis inhibitor is provided to the sample; providing a test signal while changing the temperature of the first source, so as to acquire a temperature sensitivity coefficient through a method for measuring a first ESR center-frequency variation of the antibody-fluorescent nanodiamond bound to an organelle of the cell; providing a test signal to the second source so as to measure a second ESR center-frequency variation of the antibody-fluorescent nanodiamond bound to the organelle of the cell; and estimating the temperature of the organelle bound to the antibody-fluorescent nanodiamond in the second source by a method for calculating a temperature variation of the organelle bound to the antibody-fluorescent nanodiamond in the second source by using the temperature sensitivity coefficient and the second ESR center-frequency variation.

This invention discloses an ECL biosensor and its preparation and detection methods. The preparation method includes: preparing an electrode with PANI, an AuNPs solution, and an SA@Ru-M1 solution; adding the AuNPs solution to the surface of the electrode with deposited PANI and incubating; adding a pretreated SYL3C solution to the cleaned electrode surface and incubating to obtain an aptamer-modified electrode; immersing the aptamer-modified electrode in BSA solution; then adding the cell test solution to the electrode surface; after incubation at a constant temperature, performing cell fixation and permeabilization; finally, adding the SA@Ru-M1 solution to the electrode surface and incubating to obtain the ECL biosensor. The ECL biosensor prepared by this invention has high cell capture efficiency, high detection accuracy, and strong anti-interference ability, and can achieve highly sensitive and specific quantitative detection of MCF-7 cells.

The present disclosure relates to cell immobilized beads and a method for preparing the same and, more specifically, to cell-immobilized beads wherein the conversion activity of cells contained in the immobilized beads is excellent and wherein the conversion activity is maintained even during distribution and storage processes, a method for preparing the cell-immobilized beads, and a use of the conversion activity of the beads.

The present invention relates to a technology for gelling a sample (e.g., exfoliated human cells) in a vial, safely transporting the sample to a testing center, restoring the sample to its original state (e.g., converting it to a liquid phase) at the testing center, and then examining the sample. More specifically, the present invention relates to a technology for converting a sample-preserving liquid contained in a vial into a jelly state, safely transporting the sample to a testing center without deforming it, redissolving the sample-preserving liquid into a liquid phase at the testing center, and then examining the sample. The present invention is advantageous in that gelling of the cell-fixing liquid filled in the vial is simplified because the cell-fixing liquid is gelled by mixing a selected solution with a selected material.

A cell chip is produced by performing a fixing step for immersing cells in a cell fixing liquid, an arrangement step for discharging a cell suspension containing the fixed cells and a liquid from an inkjet-mode discharge part onto a discharge target medium such as a membrane to arrange droplets of the cell suspension on the discharge target medium, and a dyeing step for dyeing a cell-derived component, in this order.

The application discloses a short-range nitrification and denitrification reactor and a running method thereof. The reactor comprises, in sequence, a water inlet tank, a short-range nitrification and denitrification reaction tower, a sedimentation tank, an intermediate water tank, a cell immobilization anaerobic ammonia oxidation reactor and a water outlet tank. The running method of the reactor comprises the following steps: S1, sludge inoculation; S2, nitrification and denitrification treatment: S2-1, pretreatment; S2-2, continuous treatment; S2-3, post-treatment; and S3, cell immobilization anaerobic ammonia oxidation treatment. The application combines the short-range nitrification and denitrification technology and the cell immobilization anaerobic ammonia oxidation technology and applies the combination to the treatment of high-ammonia-nitrogen wastewater. Compared with the traditional nitrification and denitrification method, the oxygen supply amount is saved by 40-60%, the COD demand amount is reduced by 100%, the sludge yield is reduced by 60-90% through recycling and reusing of the sludge in the sedimentation tank, and the NO2 ‑ ‑N / NH4 + ‑N ratio is stable and convenient to control compared with the general short-range nitrification and denitrification-anaerobic ammonia oxidation technology.

The application belongs to the technical field of biology, and discloses a stabilizing protective agent, a cell antigen dry sheet, and a preparation method and application of the dry sheet. The stabilizing protective agent comprises 0.4% glycerol in volume fraction, 0.1% gelatin in mass volume fraction, 0.1 mM EDTA, 0.03% trehalose in mass volume fraction, and 100 muM resveratrol. The stabilizing protective agent is used to prepare the cell antigen dry sheet and is applied in CBA detection. The stabilizing protective agent has good permeability, penetrates into the cell interior through the cell membrane, provides protection inside and outside the cell, has moisturizing and antioxidant properties, can prevent cell deformation and cell fixation layer from falling off, well maintains the stability and reliability of antigen protein, and the prepared cell antigen dry sheet can be stored for a long time without damaging the antigenicity of the membrane signal index. When a positive sample is detected, obvious green fluorescent cells can be seen, and red fluorescent quenching does not occur, so that the co-localization of red fluorescence and green fluorescence can be observed.

Described herein are systems and methods for sorting and selecting cells by immobilizing or enclosing cells in degradable hydrogel chambers, including a method of sorting cells comprising: (a) providing a fluidics device comprising: (i) a channel comprising a surface, (ii) a spatial energy modulation element in optical communication with the surface, and (iii) a detector in optical communication with the surface and in operable association with the spatial energy modulating element, the detector identifying cells and determining positions thereof on the surface; (b) loading the channel with cells and one or more polymer precursors so that the cells are disposed on or adjacent to the surface; (c) immobilizing one or more cells selected based on one or more optical signals therefrom by synthesizing one or more gel structures enclosing each of the one or more cells by projecting light into the channel with the spatial energy modulating element such that the projected light causes cross-linking of the one or more polymer precursors to form gel structures, wherein the positions of the gel structures in the channel are determined by the positions of the cells enclosed thereby identified by the detector; and (d) removing from the channel unselected cells.

The invention discloses an HEK293T cell strain capable of stably expressing PLA2R and application of the HEK293T cell strain. The HEK293T cell strain is preserved in the China Center for Type Culture Collection (CCTCC), and the preservation number is CCTCC NO: C202601. After the cell strain is optimized by a cell immobilization scheme, BSA (bovine serum albumin) is added into an immobilization solution, so that PLA2R protein expressed on the cell strain can be protected, the positive rate of the cell strain in enzyme repair immunohistochemical staining is increased, and the cell strain can be applicable to an IHC staining scheme with enzyme repair of a full-automatic immunohistochemical machine such as LYNX480 equipment; therefore, the cell strain can be widely used as a positive cell quality control product of PLA2R.

The invention belongs to the technical field of biomedical analysis, and relates to a cell sorting and detecting system integrating microfluidic control and a terahertz transmission line and a method thereof. A cell sorting area of the system comprises: a cell injection chamber for introducing a multi-cell mixed culture solution to be sorted; the microfluid channel is used for realizing primary cell sorting based on a magnetic field; the capture trap microstructure array is used for capturing the free MNPs and realizing the separation of the free MNPs and the target cells with the MNPs markers; the transmission line detection area comprises a fixing structure used for fixing a to-be-detected cell in a sensing area of the transmission line; the transmission line detection unit is used for detecting the characteristic spectrums of the labeled cells and the unlabeled cells in situ in real time; an outlet collection chamber is also included. High integration of cell sorting and electrical detection is achieved, high-sensitivity detection of terahertz waves and accurate sample control of microfluidics can be combined, and higher-throughput, lower-damage and more accurate sample analysis and sorting are achieved.

The utility model discloses a cell stationary liquid device for flow type detection, which comprises a magnetic stirrer, a mixing bottle arranged at the top of the magnetic stirrer, a sample inlet arranged at the top of the mixing bottle, a limit cylinder arranged at the top of the inner side of a mounting rack, and a nozzle connected with the inner side of the limit cylinder through a driving mechanism, one side of the spray head is connected with the micro-flow pump through a hose, the other end of the micro-flow pump is connected with the bottom of the light-proof liquid storage tank through a hose, and a control panel is mounted on one side of the mounting frame, the light-proof liquid storage tank can prevent degradation of photosensitive stationary liquid, and the micro-flow pump can extract the stationary liquid and inject the stationary liquid into the mixing bottle through the spray head; the driving mechanism can push the spray head to be automatically connected with the sample inlet in an inserted mode, the magnetic stirrer can drive the stirrer in the mixing bottle to rotate, so that stationary liquid and cell suspension are evenly mixed, efficient, accurate and pollution-free flow cytometry cell fixing operation is achieved through the structure, and experiment repeatability and the cell survival rate are remarkably improved.

The utility model discloses a solar module for fixing and pre-processing a battery piece, which comprises a solar module main body, and the surface of the solar module is provided with an inner hexagonal cylinder head and a plurality of limiting blocks; the solar module body is composed of tempered glass, a photovoltaic cell module, a first EVA plate, a frame, a back plate, a mounting plate and a second EVA plate. A plurality of fixing grooves are formed in the upper end of the mounting plate at equal intervals, a plurality of mounting grooves are formed in the lower end of the mounting plate at equal intervals, two pre-fixing mechanisms are slidably connected into each mounting groove, an adjusting rod is rotatably connected to the middle of one end of the mounting plate, and a plurality of fixing blocks are fixedly connected to the lower end of the photovoltaic cell assembly at equal intervals. The middle parts of the lower ends of photovoltaic cells of the photovoltaic cell assembly are fixedly connected with a plurality of fixing blocks. And the fixing plates in the mounting grooves are close to each other through rotation of the adjusting rods to clamp the fixing blocks, so that pre-fixation of the battery pieces of the photovoltaic battery assembly is realized.

The urine preservative liquid provided by the application comprises a pH buffer 6-10 mmol / L, a DNA enzyme inhibitor 0.2-0.4 mol / L, a preservative 0.1-0.3 mol / L, a cell fixing agent 5%-15% by mass fraction, and a formaldehyde quencher 0.3-1.0 mol / L; the urine preservative liquid avoids mutual interference between components, influences the functions of the components, still has respective corresponding protection effects between 4 DEG C and 40 DEG C, and has no direct influence on nucleic acids in urine, so that the preservative liquid can still maintain the urine unspoiled at 4 DEG C-40 DEG C, and guarantees the relative content of cfDNA.

This invention relates to the field of medical testing, and particularly to a Wright-Gymsza staining solution and its application. The Wright-Gymsza staining solution of this invention comprises staining solution A and staining solution B. Staining solution A mainly consists of eosin Y, azurite-eosin A, methylene blue, cell fixative, and stabilizer, while staining solution B is a buffer solution. Experiments have shown that the Wright-Gymsza staining solution of this invention can stain normally for 27 months after preparation, solving the technical problems of uneven staining and dye residue on the staining platform after a period of time after opening.

The invention relates to the technical field of single-cell gel electrophoresis, and discloses a single-cell gel electrophoresis first-layer gel spreading method, which comprises the steps of gel solution preparation, vertical impregnation, gradient temperature control solidification, storage and the like. According to the glue spreading method for the first layer of the single-cell gel electrophoresis, the physical performance of the glue layer is further optimized through two-stage temperature control of low-temperature solidification of gradient temperature control solidification and secondary heating stabilization, a 50 ml beaker is adopted as a dissolving container, and the uniformity of the solution volume and temperature diffusion can be ensured; compared with traditional natural cooling for 4 h or above, the total consumed time can be shortened to 2 h, meanwhile, the influence of room temperature fluctuation on the gel forming quality can be avoided, compared with a traditional method, the thickness of a gel layer prepared through the method is more uniform, background noise during electrophoresis can be reduced, the cell fixation success rate is higher, the experiment repetition rate is higher, and the method is suitable for large-scale popularization and application. The sensitivity and the accuracy of DNA damage detection are obviously improved.

The invention provides a structured cell growth matrix or assembly comprising a one or more spacer layers and one or more cell immobilization layers. The invention further provides a bioreactor comprising said matrix or assembly.

The application provides a cell climbing sheet processing reagent for a Ranvier node / paranode antibody spectrum detection kit (cellular immunofluorescence method), a method and application thereof, and relates to the technical field of in-vitro detection. The cell climbing sheet processing reagent comprises a cell fixing solution with a specific composition and an antigen repair composition after cell fixing, wherein the cell fixing solution is composed of paraformaldehyde, formaldehyde, acetic acid, raffinose and a buffer solution, and the antigen repair composition after cell fixing is composed of ammonium sulfate, hydroxylamine hydrochloride, Brij 78, TCEP, ProClin 300 and a buffer solution. Through the synergistic effect of the two, the natural conformation of the target antigen is maximally retained, and non-specific interference is reduced, so that precise and efficient detection of the Ranvier node / paranode antibody spectrum NF155, NF186, CNTN1, CASPR1 and MAG can be realized, the clinical diagnosis demand is met, and reliable technical support is provided for precise typing of autoimmune Ranvier node disease.