Kit for verifying adipose-derived stem cells

A technology of mesenchymal stem cells and kits, applied in the direction of cell culture active agents, animal cells, vertebrate cells, etc., can solve the problems of affecting cell identification results, time-consuming, low induction efficiency, etc., achieve accurate identification results, and shorten induction differentiation The time, the effect of improving the efficiency of induction of differentiation
CN105866431AActive Publication Date: 2016-08-17天津欣普赛尔生物医药科技有限公司
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
天津欣普赛尔生物医药科技有限公司
Publication Date
2016-08-17

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Abstract

The invention discloses a kit for verifying adipose-derived stem cells. The kit comprises a flow phenotype detection reagent set, cell fixation liquid, an ordinary culture medium, an adipogenesis induction and detection reagent set, an osteogenesis induction and detection reagent set and a chondrogenesis induction and detection reagent set. Usually, conventional osteogenesis induction and adipogenesis induction need about 24 days, consumed time is long, and induction efficiency is low; by the kit, the time for osteogenesis induction and adipogenesis induction is shortened to 18 days, induction efficiency is improved, and induction time is shortened; conventional flow detection mainly aims at universal surface markers of mesenchymal stem cells, but the adipose-derived stem cells have positively-expressed surface markers CD49d and negatively-pressed CD106 which are different from the surface markers of other mesenchymal stem cells; CD49d antibodies and CD106 antibodies are added into the kit, and accordingly, accurate verification results can be acquired.
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Description

technical field

[0001] The invention relates to a kit, especially a kit for identifying fat mesenchymal stem cells. Background technique

[0002] At present, more and more researches are using adipose-derived mesenchymal stem cells for clinical use, and the development prospect of adipose-derived mesenchymal stem cells is getting better and better. The International Society for Cell Therapy (ISCT) stipulates that the identification of mesenchymal stem cells includes three aspects: morphology, flow phenotype, and ability to induce differentiation. Traditional mesenchymal stem cell identification methods are relatively mature, but the process of induction and differentiation takes a long time, and the induction efficiency is low, which affects the final cell identification results. Contents of the invention

[0003] The technical problem to be solved by the present invention is to provide a kit for identifying adipose-derived mesenchymal stem cells which improves the induct...

Claims

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