Kit for identification of adipose-derived mesenchymal stem cells

A technology of mesenchymal stem cells and kits, applied in the direction of cell culture active agents, animal cells, vertebrate cells, etc., can solve the problems of affecting cell identification results, time-consuming, low induction efficiency, etc., achieve accurate identification results, and shorten induction differentiation The time, the effect of improving the efficiency of induction of differentiation

Active Publication Date: 2018-01-23
天津欣普赛尔生物医药科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Traditional mesenchymal stem cell identification methods are relatively mature, but the process of inducing differentiation takes a long time, and the induction efficiency is low, which affects the final cell identification results

Method used

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  • Kit for identification of adipose-derived mesenchymal stem cells
  • Kit for identification of adipose-derived mesenchymal stem cells
  • Kit for identification of adipose-derived mesenchymal stem cells

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0061] The adipogenic induction and differentiation reagent set: including 100mL adipogenic induction medium and 10mL adipogenic differentiation detection reagent, the adipogenic induction medium is containing 2mmoL / L glutamine, 20μg / mL IGF-2, 5mmol / L 3- Glycerol phosphate, 1mmoL / L beclomethasone, 0.5mmoL / L isobutylmethylxanthine, 200μmoL / L indomethacin, 50μmoL / L acetyl CoA, AMEM medium with a volume fraction of 10% fetal bovine serum;

[0062] The osteogenic differentiation induction and detection reagent set: including 100mL osteogenic induction medium and 10mL osteogenic differentiation detection reagent, the osteogenic induction medium contains 2mmoL / L glutamine, 2mmoL / L beclomethasone, 20μg / mL TGF-β, 10mmol / L β-sodium glycerophosphate, 25mmol / L vitamin D3, 30mmol / L vitamin C, AMEM medium with a volume fraction of 10% fetal bovine blood;

[0063] The chondrogenic differentiation induction and detection reagent set: including 100mL chondrogenic induction medium and 10mL cho...

Embodiment 2

[0093] The adipogenic induction and differentiation reagent set: including 100mL adipogenic induction medium and 10mL adipogenic differentiation detection reagent, the adipogenic induction medium is containing 0.2mmoL / L glutamine, 1μg / mLIGF-2, 2mmol / L 3- Glycerol phosphate, 0.25mmoL / L beclomethasone, 0.1mmoL / L isobutylmethylxanthine, 100μmoL / L indomethacin, 1μmoL / L acetyl CoA, AMEM medium with a volume fraction of 2% fetal bovine serum;

[0094] The osteogenic differentiation induction and detection reagent set: including 100mL osteogenic induction medium and 10mL osteogenic differentiation detection reagent, the osteogenic induction medium contains 0.5mmoL / L glutamine, 0.5mmoL / L beclomethasone, 5 μg / mL TGF-β, 2mmol / L β-sodium glycerophosphate, 15mmol / L vitamin D3, 20mmol / L vitamin C, AMEM medium with a volume fraction of 2% fetal bovine blood;

[0095] The chondrogenic differentiation induction and detection reagent set: including 100mL chondrogenic induction medium and 10mL...

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Abstract

The invention discloses a kit for verifying adipose-derived stem cells. The kit comprises a flow phenotype detection reagent set, cell fixation liquid, an ordinary culture medium, an adipogenesis induction and detection reagent set, an osteogenesis induction and detection reagent set and a chondrogenesis induction and detection reagent set. Usually, conventional osteogenesis induction and adipogenesis induction need about 24 days, consumed time is long, and induction efficiency is low; by the kit, the time for osteogenesis induction and adipogenesis induction is shortened to 18 days, induction efficiency is improved, and induction time is shortened; conventional flow detection mainly aims at universal surface markers of mesenchymal stem cells, but the adipose-derived stem cells have positively-expressed surface markers CD49d and negatively-pressed CD106 which are different from the surface markers of other mesenchymal stem cells; CD49d antibodies and CD106 antibodies are added into the kit, and accordingly, accurate verification results can be acquired.

Description

technical field [0001] The invention relates to a kit, especially a kit for identifying fat mesenchymal stem cells. Background technique [0002] At present, more and more researches are using adipose-derived mesenchymal stem cells for clinical use, and the development prospect of adipose-derived mesenchymal stem cells is getting better and better. The International Society for Cell Therapy (ISCT) stipulates that the identification of mesenchymal stem cells includes three aspects: morphology, flow phenotype, and ability to induce differentiation. Traditional mesenchymal stem cell identification methods are relatively mature, but the process of induction and differentiation takes a long time, and the induction efficiency is low, which affects the final cell identification results. Contents of the invention [0003] The technical problem to be solved by the present invention is to provide a kit for identifying adipose-derived mesenchymal stem cells which improves the induct...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/68G01N33/569C12N5/077
CPCC12N5/0653C12N5/0654C12N5/0655C12N2500/38C12N2500/40C12N2500/42C12N2500/84C12N2501/105C12N2501/15C12N2501/90C12N2501/998C12N2501/999C12N2502/1382G01N33/56966G01N33/6854
Inventor 刘俊江鲁振宇黄文敬韩洪起张冰晶秦臻徐悦
Owner 天津欣普赛尔生物医药科技有限公司
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