Kit for identification of adipose-derived mesenchymal stem cells
A technology of mesenchymal stem cells and kits, applied in the direction of cell culture active agents, animal cells, vertebrate cells, etc., can solve the problems of affecting cell identification results, time-consuming, low induction efficiency, etc., achieve accurate identification results, and shorten induction differentiation The time, the effect of improving the efficiency of induction of differentiation
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Embodiment 1
[0061] The adipogenic induction and differentiation reagent set: including 100mL adipogenic induction medium and 10mL adipogenic differentiation detection reagent, the adipogenic induction medium is containing 2mmoL / L glutamine, 20μg / mL IGF-2, 5mmol / L 3- Glycerol phosphate, 1mmoL / L beclomethasone, 0.5mmoL / L isobutylmethylxanthine, 200μmoL / L indomethacin, 50μmoL / L acetyl CoA, AMEM medium with a volume fraction of 10% fetal bovine serum;
[0062] The osteogenic differentiation induction and detection reagent set: including 100mL osteogenic induction medium and 10mL osteogenic differentiation detection reagent, the osteogenic induction medium contains 2mmoL / L glutamine, 2mmoL / L beclomethasone, 20μg / mL TGF-β, 10mmol / L β-sodium glycerophosphate, 25mmol / L vitamin D3, 30mmol / L vitamin C, AMEM medium with a volume fraction of 10% fetal bovine blood;
[0063] The chondrogenic differentiation induction and detection reagent set: including 100mL chondrogenic induction medium and 10mL cho...
Embodiment 2
[0093] The adipogenic induction and differentiation reagent set: including 100mL adipogenic induction medium and 10mL adipogenic differentiation detection reagent, the adipogenic induction medium is containing 0.2mmoL / L glutamine, 1μg / mLIGF-2, 2mmol / L 3- Glycerol phosphate, 0.25mmoL / L beclomethasone, 0.1mmoL / L isobutylmethylxanthine, 100μmoL / L indomethacin, 1μmoL / L acetyl CoA, AMEM medium with a volume fraction of 2% fetal bovine serum;
[0094] The osteogenic differentiation induction and detection reagent set: including 100mL osteogenic induction medium and 10mL osteogenic differentiation detection reagent, the osteogenic induction medium contains 0.5mmoL / L glutamine, 0.5mmoL / L beclomethasone, 5 μg / mL TGF-β, 2mmol / L β-sodium glycerophosphate, 15mmol / L vitamin D3, 20mmol / L vitamin C, AMEM medium with a volume fraction of 2% fetal bovine blood;
[0095] The chondrogenic differentiation induction and detection reagent set: including 100mL chondrogenic induction medium and 10mL...
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