Kit for identifying mesenchymal stem cells

A technology of mesenchymal stem cells and kits, applied in the field of kits for identifying umbilical cord mesenchymal stem cells, can solve the problems of affecting cell identification results, time-consuming, low induction efficiency, etc., achieve fast and accurate identification results, and shorten the time for induction and differentiation , the effect of improving the efficiency of inducing differentiation

Inactive Publication Date: 2016-08-24
TIANJIN PURUI SAIER BIOLOGICAL TECH CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Traditional mesenchymal stem cell identification methods are relatively mature, but the process of inducing differentiation takes a long time, and the induction efficiency is low, which affects the final cell identification results

Method used

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  • Kit for identifying mesenchymal stem cells
  • Kit for identifying mesenchymal stem cells
  • Kit for identifying mesenchymal stem cells

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Experimental program
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Effect test

Embodiment 1

[0061] The adipogenic differentiation induction and detection reagent set: including 100mL adipogenic induction medium and 10mL adipogenic differentiation detection reagent, the adipogenic induction medium contains 2mmoL / L glutamine, 20μg / mL IGF-1, 10mmol / L3 -Glycerol phosphate, 1.5mmoL / L betamethasone, 0.5mmoL / L isobutylmethylxanthine, 200μmol / L indomethacin, 100μmol / L acetyl CoA, DMEM / F12 medium containing 10% fetal calf serum by volume fraction ;

[0062] The osteogenic differentiation induction and detection reagent set: including 100mL osteogenic induction medium and 10mL osteogenic differentiation detection reagent, the osteogenic induction medium contains 2mmoL / L glutamine, 1μmol / L betamethasone, 10μg / mL TGF-β, 10mmol / Lβ-sodium glycerophosphate, 10mmol / L vitamin D3, 20mmol / L vitamin C, DMEM / F12 medium containing 10% fetal bovine serum by volume fraction;

[0063] The chondrogenic differentiation induction and detection reagent set: including 100mL chondrogenic inductio...

Embodiment 2

[0094] The adipogenic differentiation induction and detection reagent set: including 100mL adipogenic induction medium and 10mL adipogenic differentiation detection reagent, the adipogenic induction medium contains 0.2mmoL / L glutamine, 1μg / mLIGF-1, 5mmol / L Glycerol 3-phosphate, 0.5mmoL / L betamethasone, 0.1mmoL / L isobutylmethylxanthine, 100μmoL / L indomethacin, 20μmoL / L acetyl CoA, cultured in DMEM / F12 containing 2% fetal bovine serum base;

[0095] The osteogenic differentiation induction and detection reagent set: including 100mL osteogenic induction medium and 10mL osteogenic differentiation detection reagent, the osteogenic induction medium contains 0.5mmoL / L glutamine, 0.15μmol / L betamethasone, 3μg / mL TGF-β, 2mmol / Lβ-sodium glycerophosphate, 5mmol / L vitamin D3, 10mmol / L vitamin C, DMEM / F12 medium containing 2% fetal bovine serum by volume fraction;

[0096] The chondrogenic differentiation induction and detection reagent set: including 100mL chondrogenic induction medium ...

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Abstract

The invention discloses a kit for identifying mesenchymal stem cells. The kit comprises a flowing phenotypic detection reagent suit, cell fixing liquid, a general culture medium, an adipogenesis differentiation induction and detection reagent suit, an osteogenesis differentiation induction and detection reagent suit and a chondrogenesis differentiation induction and detection reagent suit. The conventional osteogenesis and adipogenesis induction differentiation generally need about 24 days, so that the time is longer, and the induction efficiency is lower. The kit provided by the invention has the advantages that the osteogenesis and adipogenesis induction differentiation time is shortened to be 18 days; the induction differentiation efficiency is improved; the induction differentiation time is shortened; a flowing detection antibody is provided, so that a faster and more accurate authentication result is obtained.

Description

technical field [0001] The invention relates to a kit, especially a kit for identifying umbilical cord mesenchymal stem cells. Background technique [0002] At present, more and more studies are using umbilical cord-isolated mesenchymal stem cells for clinical use, and the development prospects of umbilical cord mesenchymal stem cells are getting better and better. The International Society for Cell Therapy (ISCT) stipulates that the identification of mesenchymal stem cells includes three aspects: morphology, flow phenotype, and ability to induce differentiation. Traditional mesenchymal stem cell identification methods are relatively mature, but the process of induction and differentiation takes a long time, and the induction efficiency is low, which affects the final cell identification results. Contents of the invention [0003] The technical problem to be solved by the present invention is to provide a kit for identifying umbilical cord mesenchymal stem cells which imp...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68C12N5/0775
CPCG01N33/5073C12N5/0668
Inventor 鲁振宇刘俊江韩洪起张冰晶秦臻徐悦黄文敬
Owner TIANJIN PURUI SAIER BIOLOGICAL TECH CO LTD
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