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165 results about "Cell fixing" patented technology
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Process and installations for separation of magnetic particles in a fluid for biological analysis, and application of said process
InactiveUS6143577AMaterial thermal conductivityPreparing sample for investigationEngineeringBacteria
PCT No. PCT / FR97 / 00794 Sec. 371 Date May 15, 1998 Sec. 102(e) Date May 15, 1998 PCT Filed May 5, 1997 PCT Pub. No. WO97 / 42503 PCT Pub. Date Nov. 13, 1997A process for magnetic immunoseparation of cells, in particular bacteria, fetal cells, stock cells of bone marrow and circulating cancerous cells, of the type consisting in affixing the target cells on paramagnetic balls and in causing a magnetic field to act on a sample containing the affixed cells, the free cells and the surplus paramagnetic balls, in order to isolate the paramagnetic balls, in that the sample is caused to circulate in a tube the section of which is much less than the length over which the magnetic field is applied.
Owner:DELTA INSTR BV
Circulating tumor cell detection kit, preparing method thereof and application thereof
InactiveCN105115878AExtended shelf lifeFor long-term storageIndividual particle analysisNucleotideSingle strand
The invention particularly relates to a circulating tumor cell detection kit, a preparing method thereof and an application thereof. The circulating tumor cell detection kit comprises a kit body, detection reagents and a micro-fluidic chip, wherein the detection reagents and the micro-fluidic chip are arranged in the kit body. The micro-fluidic chip is formed by a PDMS substrate and a glass slide in an attached mode. A micro-channel is distributed on the surface of the PDMS substrate, and a fishbone-shaped alternate type mixing structure is arranged on the bottom face of the micro-channel. After the PDMS substrate and the glass slide are bonded, a microfluid channel is formed on the surface of the micro-channel and the surface of the glass slide, and capturing probes are loaded on the surface of the micro-channel. The invention further provides the preparing method of the micro-fluidic chip and a method for detecting circulating tumor cells through the kit. Compared with the prior art, the circulating tumor cell detection kit and the methods have the advantages that the capture probes are loaded on all the surfaces of the micro-channel, and therefore the capturing efficiency is improved; as the micro-fluidic chip is modified through single strand nucleotide, the storage time of the chip is prolonged; in addition, a cell-fixing-free straining method is provided, and therefore convenience is provided for further cell gene sequencing.
Owner:SHANGHAI JIAO TONG UNIV
Cell selection apparatus, and cell selection method using the same
InactiveUS20110033910A1Avoid difficult choicesMaintain activityBioreactor/fermenter combinationsBiological substance pretreatmentsCell selectionEngineering
The cell selection apparatus includes: a cell selection vessel which has a pair of electrodes and a sheet-like insulating material having a plurality of micropores, with a recognition molecule bindable to the specific substance being disposed on a bottom face of the micropore; and a power supply, wherein the power supply includes a cell-immobilization power supply and a cell-taking power supply. The cell selection method uses the cell selection apparatus, including; introducing cells into a cell selection area; immobilizing these cells in the micropores; effecting a binding reaction between the specific substance and the recognition molecule; thereafter, taking out a cell of which the specific substance is not or is weakly bound to the recognition molecule, from the micropore; otherwise alternatively, leaving a cell of which the specific substance on the surface of the cell is strongly bound to the recognition molecule, behind in the micropore.
Owner:TOSOH CORP
Capacitor module
InactiveUS20110007480A1High strengthIncrease freedomProtecting/adjusting hybrid/EDL capacitorDouble layer capacitorsCapacitanceFixed capacitor
A capacitor module is provided with a plurality of capacitor cells each having a capacitor and a capacitor case provided with a first closed-bottomed screw hole with an opening on a bottom surface for housing the capacitor, a metallic cell-fixing body having a through-hole in communication with the first screw hole to which the capacitor cells are fixed by inserting a cell screw in the through-hole and the first screw hole, an insulator made of a thermally conductive insulating material and provided between the capacitor cells and the cell-fixing body for insulating the capacitor cells from the cell fixing body, and a metallic heat-dissipating body having a second closed-bottomed screw hole in which a cell-fixing body screw is inserted and having a flow passage for causing a cooling medium to flow on a rear surface side of a surface on which the second screw hole is provided.
Owner:KOMATSU LTD
Circulating tumor cell detection kit and application thereof
InactiveCN105785005AAccurate detectionEfficient enrichmentMaterial analysisLymphatic SpreadFluorescence
The invention relates to a circulating tumor cell detection kit and application thereof. The circulating tumor cell detection kit comprises a chip, sample diluent, a cell separation fluid, a cell trapping agent, a cell cleaning solution, a cell fixing agent, antibody diluent, a cell penetration agent and a fluorescence dye. The application method of the circulating tumor cell detection kit mainly comprises the several steps of separation of circulating tumor cells, trapped antibody coating, trapping of the circulating tumor cells and immuno-fluorescent staining of the circulating tumor cells. The circulating tumor cell detection kit is mainly used for tumor prognosis, reappear, metastasis, curative effect monitoring, early diagnosis and early warning, monitoring of curative effect and tumor progression situation, and utilizes the molecular subtyping, screening specificity and other characteristics of the circulating tumor cells to conduct targeted research on circulating tumor cell sensitive drug so as to supplement tumor metastasis, relapse and drug resistance mechanisms and guide individualized medical treatment.
Owner:杭州华得森生物技术有限公司
Immobilization of cells in a matrix formed by biocompatible charged polymers under laminar flow conditions
InactiveUS20060019361A1Improve stabilityGood flexibilityArtificial cell constructsCell culture supports/coatingCharged polymersCell fixing
This invention relates to a method for immobilizing cells under laminar flow conditions in a matrix formed by biocompatible charged polymers, to a flow device for the use with the method of the present invention, and to uses of the polymer matrices containing the cells immobilized using the method of the present invention.
Owner:AGENCY FOR SCI TECH & RES
Liquid-base cell preservation liquid as well as preparation method and application thereof
The invention discloses liquid-base cell preservation liquid as well as a preparation method and application thereof, relating to the technical field of biology. The liquid-base cell preservation liquid contains the following components in parts by weight: 12-18 parts of a fixing agent, 2-8 parts of an anti-coagulation agent, 2-10 parts of a buffer agent, 0.5-0.8 part of an ion strength maintaining agent, 2-8 parts of an antimicrobial reagent, 0.05-0.2 part of a mucus dissolving agent and 0.1-0.4 part of a cleanser. The liquid-base cell preservation liquid has the advantages that the cell stability is high, cells are well fixed, the cost is low, the preserved cell forms are relatively complete, and the like. Besides, the invention further provides the preparation method of the liquid-basecell preservation liquid and application of the liquid-base cell preservation liquid in human body cast-off cells.
Owner:生工生物工程(上海)股份有限公司
Composite conformable pressure vessel
InactiveUSRE41142E1Trend downIncrease pressureGas handling applicationsLarge containersLine tubingCompressed natural gas
A pressure vessel for holding a pressurized fluid such as compressed natural gas (“CNG”) includes two end cells and zero or more interior cells. The cell geometry ensures that the cells meet one another at tangential circular surfaces, thereby reducing the tendency of adjacent cells to peel apart. A web secured about the cells includes two sheets that are tangent to the cells. Unused volumes between the cells and the web contain wedges of foam or rubber. A valve provides fluid communication between the interior of the pressure vessel and a pressurized fluid line. The filled weight of one pressure vessel does not exceed the filled weight of a conventional gasoline tank that occupies substantially the same space as the pressure vessel. The pressure vessel may be configured with exterior recesses for engaging conventional gasoline tank straps.
Owner:NORTHROP GRUMMAN SYST CORP
Capacitor module
InactiveCN101981638AHigh strengthIncrease freedomProtecting/adjusting hybrid/EDL capacitorDouble layer capacitorsCapacitorCell fixing
A capacitor module having increased strength as a whole and having increased freedom in design of a cooling medium flow passage to be formed in the module. The capacitor module is provided with capacitor cells each having a capacitor and a capacitor case which is equipped with first closed-bottomed screw holes having respective openings in the bottom surface of the capacitor case and which houses the capacitor, a metallic cell-fixing body having through-holes communicating with the first screw holes and to which the capacitor cells are fixed by engaging cell screws into the through-holes and the first screw holes, an insulator consisting of a thermally conductive insulating material, installed between the cell-fixing body and the capacitor cells and, and insulating between the cell-fixing body and the capacitor cells, and a metallic heat-dissipating body having second closed-bottomed screw holes into which screws for the cell-fixing body are engaged and also having a flow passage for causing the cooling medium to flow to the rear side of that surface of the heat-dissipating body in which the second screw holes are formed.
Owner:KOMATSU LTD
Light curing fixative
InactiveUS20070134798A1High densityHigh refractive indexAnalysis using chemical indicatorsSamplingLight cureCell fixing
The invention relates to a cell fixative that can be used in the preparation of slides for investigation by microscopy techniques. In particular the present invention provides a cell fixative that contains a light curable mounting medium.
Owner:CYTYC CORP
Liquid-based cell preservation liquid composite and preparation method thereof
InactiveCN102318597AKeep shapeImprove diagnostic accuracyDead animal preservationFood additiveSodium diacetate
A liquid-based cell preservation liquid composite is characterized by comprising the following components in percentage by weight: cell fixing agent: 2-15% of formaldehyde, preservative: 0.1-5% of sodium diacetate, anticoagulant: 2-10% of sodium citrate, buffering agent: 0.5-2% of sodium chloride, chelating agent: 0.002-0.5% of ethylene diamine tetraacetic acid (EDTA), hemolytic agent: 0.5-10% ofglacial acetic acid, and water: 5-80% of pure water, wherein the formaldehyde is the analytical reagent, and accounts for 37-40%; the sodium diacetate is the food additives; the sodium citrate and the sodium chloride are analytical reagents, and NaCl is more than 99.5%; and the EDTA and the glacial acetic acid are analytical reagents, and H2COOH is more than 99.5%. The invention has the advantages that the liquid-based cell preservation liquid composite can keep the cell form to the maximum extent, can improve the diagnosis accuracy and can prevent the cell form of a specimen from significantdifference at room temperature for at least three months.
Owner:上海鎏轶生物技术中心
Determining Information for Cells
InactiveUS20140065637A1Bioreactor/fermenter combinationsBiological substance pretreatmentsControl cellCell system
Systems, methods, and devices for determining information for cells are provided. The systems, methods, and devices are configured such that information for more than 100,000 cells can be determined in a single run. The devices are configured to immobilize the cells. The devices also include features that can be used by the systems and methods for determining and tracking the positions of each of the cells in the device on a cell-by-cell basis. The systems and methods are configured for substantially high resolution of the cells while the cells are immobilized in the device. In addition, environmental control subsystems are provided that can control an environment of the cells while the device is positioned in the system or the method is being performed without altering positions of the cells within the device.
Owner:KLA CORP
Microfluidic control chip for cell immobilization and solution dilution
InactiveCN101290314AStructural advantagesEasy to operateMicrobiological testing/measurementBiological testingConcentration gradientEngineering
The invention provides a micro-fluidic chip used for cell immobilization and solution dilution. The invention comprises a transparent hard substrate layer one surface of which is provided with a plurality of microchannels by means of machining, and also comprises a transparent elastic rubber layer one surface of which is provided with micro-water pools by means of machining, wherein, the one surface of the transparent hard substrate layer which is provided with the microchannels by means of machining closely clings to the one surface of the transparent elastic rubber layer which is provided with the micro-water pools by means of micro-machining; the microchannels are overlapped with the micro-water pools to form a closed cell suspension or a solution flowing path; the dimension of the prior micro-water pools is changed through selection of different structures of the micro-water pools or through external force, thereby the flow condition of the whole chip can be controlled and cell immobilization of solution dilution are realized. The micro-fluidic chip can be used on a microchip to immobilize different biological living cells or abiological particles and then realize bioanalysis by taking the cells and molecules as the basis; simultaneously, the micro-fluidic chip can control different types of solution and mix the solution according to the set proportion and the diffusion conditions, and then the solution concentration or the concentration gradient required is obtained; and the micro-fluidic chip is used for high-flux biological sample analysis.
Owner:CHONGQING UNIV
Apparatus and method for electrically contacting biological cells suspended in a liquid
InactiveUS7361500B2Improve automationAvoid disadvantagesBioreactor/fermenter combinationsBiological substance pretreatmentsElectricityBiological cell
An apparatus for electrically contacting biological cells suspended in a liquid has a substrate having at least one opening and an electrode for electrically contacting a cell immobilized above the opening. A contact unit with a contact tip is arranged below said opening. A top end of the contact tip projects into the opening in such a way that it comes to bear against a cell membrane of the immobilized cell. The contact tip has a contact channel which ends at its top end. A hydrodynamic low pressure can be exerted upon the cell membrane via the contact channel, and the electrode is electrically connected to the contact channel. In a method for electrically contacting biological cells suspended in a liquid, a cell is immobilized above an opening provided in a substrate, and the immobilized cell is contacted via at least one electrode. For contacting the immobilized cell, a hydrodynamic low pressure is generated acting on a cell membrane through a contact tip projecting into the opening.
Owner:CYTOCENTRICS BIOSCI
Evaluation method for measuring cell DNA damage caused by soluble heavy metal
ActiveCN104931473AFast and accurate simultaneous detectionThe target is accurate and intuitiveFluorescence/phosphorescenceCell stainingHigh content imaging
The invention relates to an evaluation method for measuring cell DNA damage caused by soluble heavy metal. The method includes the following steps that firstly, tested cells are processed through a soluble heavy metal solution; secondly, the processed tested cells are fixed and are subjected to cell permeabilization; thirdly, the tested cells having been subjected to cell permeabilization are dyed; fourthly, detection is performed through a high-content imaging system and analysis is performed. The evaluation method for measuring the cell DNA damage caused by the soluble heavy metal is convenient to operate, high in sensitivity and high in detection speed and has high specific pertinence, and research on the genetic toxicity of the heavy metal is supplemented and expanded.
Owner:CHINA TOBACCO YUNNAN IND
Urine preservation reagent and application thereof
InactiveCN107881213AEffective protectionReduce the impactMicrobiological testing/measurementArginineDiazolidinyl urea
The invention discloses a urine preservation reagent and an application thereof. The urine preservation reagent comprises a nucleic acid buffer, a metal ion chelating agent, an antiseptic, a cell fixing agent, and a formaldehyde quenching agent; a nucleic acid buffer is Tris-HCl, a working pH value is 7-8, the metal ion chelating agent is EDTA, the antiseptic is citric acid and / or sodium citrate,the cell fixing agent is selected from at least one of paraformaldehyde, diazolidinyl urea, imidazolidinyl urea, and diethyl urea; and the formaldehyde quenching agent is selected from at least one ofglycine, lysine, ethene diamine and arginine. The urine preservation reagent can effectively preserve free DNA in urine, degradation can be avoided, and the urine preservation reagent establishes a base for subsequently detecting the free DNA; fragmentation of cells in the urine is prevented, a genome DNA background is reduced, and free DNA detection result accuracy is guaranteed. The urine preservation reagent is the novel reagent for preserving a urine sample, and provides the guarantee for clinical detection of the urine free DNA.
Owner:GENETALKS BIO TECH CHANGSHA CO LTD
Method and device for cell modification
InactiveUS20140315311A1Increase gravityImprove developmentBioreactor/fermenter combinationsBiological substance pretreatmentsRotational axisCentrifugation
The invention relates to a cell modification device, comprising a centrifugation chamber with at least one cell modifying surface with a normal vector having an angle of 135-45° to the rotational axis of the centrifugation chamber, wherein the centrifugation chamber comprises at least one input / output port and the cells to be modified are immobilized at the cell modifying surfaces by the rotation of the centrifugation chamber at 2 to 2000 g. Furthermore, the invention relates to a method for modifying cells comprising the steps—introducing cells in a cell modification device, comprising a centrifugation chamber with at least one cell modifying surface with a normal vector having an angle of 135-45° to the rotational axis of the centrifugation chamber wherein and comprising at least one input / output port, —immobilizing the cells on the cell modifying surfaces by the rotation of the centrifugation chamber at 2 to 2000 g—maintaining the rotation of the rotation of the centrifugation chamber until the cells are modified.
Owner:MILTENYI BIOTEC
Blotting material used for specific recognition of cells, and preparation and applications thereof
The invention relates to a blotting material used for specific recognition of cells, and preparation and applications thereof. According to the blotting material, cells are taken as a template, cell culturing and cell immobilization technology are adopted to immobilize the cells on the surface of a substrate material, curing of a pre-polymerization system is carried out on the surface of the substrate material, the substrate material is removed via separation, and the template cells on the surface of an obtained cured polymer are removed so as to obtain a finished product. The blotting material is high in selectivity and stability, and can be used in identification, capturing, and enriching of cells.
Owner:DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
Battery cover plate and cover plate welding tooling, and battery preparation method
InactiveCN109860449AReduce the difficulty of security management and controlImprove safety and reliabilityFinal product manufactureSecondary cells manufactureElectrical batteryStructural engineering
The invention is applicable to the technical field of batteries, and provides a battery cover plate and a cover plate welding tooling, and a battery preparation method. The battery cover plate comprises the following parts of a cover body provided with positive and negative terminals; a positive conductive member for connecting the positive terminal and a positive tab of a cell, wherein one end ofthe positive conductive member is fixedly welded to the bottom of the positive terminal; and a negative conductive member for connecting the negative terminal and a negative tab of the cell, whereinone end of the negative conductive member is fixedly welded to the bottom of the negative terminal, and an insulating member is disposed between the positive terminal and / or the negative terminal andthe cover body. The cover plate welding tooling is used for welding the battery cover plate and the cell and comprises a first cell fixing member, a second cell fixing member and a cover plate bearingmember. The battery cover plate provided by the invention, with no need to perform welding between the positive conductive member and the positive terminal and the negative conductive member and thenegative terminal, can effectively prevent the welding dust from entering the cell, thereby greatly reducing the safety control difficulty during the battery preparation process and reducing securityrisks.
Owner:蜂巢能源(武汉)有限公司
Biological organic water-soluble fertilizer and preparation method thereof
InactiveCN101717303AStrengthen the ability to protect the benefits and discardPromote growthFertilizer mixturesDiseasePlant cell
The invention discloses a biological organic water-soluble fertilizer and a preparation method thereof. The biological organic water-soluble fertilizer is prepared by matching amino oligo-saccharide and chitosan with trace amount of elements of boron and zinc, degrading the chitosan by acetic acid, mixing amino oligosaccharin and adding boron powder and zinc powder. The biological organic water-soluble fertilizer can improve the self resisting and defending capability of plants of early harm of disease, has the functions of fixing and permeating cells of the plants, i.e. enhancing the permeability of the cells after polygalacturonic acid of plant cells is combined and increasing the leakage of low-molecule compound, thereby adjusting the growth of plants in the metabolism path in plant bodies, i.e. enhancing the tillering capability of crops, adjusting growing development of roots, stems, leaves and flowers and influencing the size and the number of reproduction organs of the plants.
Owner:杨宝森 +1
Method of Immobilizing Protein, Protein Chip, Method of Immobilizing Cell and Cell Chip
InactiveUS20080248972A1Effectively fixedGood reproducibilityPeptide/protein ingredientsAlbumin peptidesCell chipNuMA Protein
It is intended to provide a novel method of immobilizing a protein and a protein chip, by which the protein can be immobilized at a high reproducibility while preventing the protein from inactivation without resort to a large-scaled apparatus and the protein can be immobilized even in a microchannel. Further, by using a cell adhesive protein as the protein to be immobilized, it is also possible to use a cell as a target and to provide a method of immobilizing a cell and a cell chip, by which a cell can be immobilized in an arbitrary region on a substrate.
Owner:TOHOKU TECHNO ARCH CO LTD
Complete gluing apparatus and gluing technology of new energy automobile assembled battery
ActiveCN107046147AReduce labor intensityReduce secondary pollutionAssembling battery machinesFinal product manufactureNew energyEngineering
The invention relates to a complete gluing apparatus and a gluing technology of a new energy automobile assembled battery. The apparatus comprises a main frame (1), the main frame (1) is provided with a conveying device (2), a feeding device (3), a gluing device (4) and a cell grasping and stacking device (5) are sequentially arranged above the conveying device (2), the main frame (1) is provided with a cell overturning device (6), the conveying device (2) is provided with a positioning sensor (7), and the positioning sensor (7) is connected with a controller (8); the cell grasping and stacking device grasps cells to the cell overturning device; and the cell overturning device overturns the cells to a cell fixing and clamping assembly, the cells are conveyed to the gluing device through a conveying track and are glued, and the cell grasping and stacking device grasps and stacks the cells. The apparatus and the technology have the characteristics of improvement of the gluing quality, reduction of the labor intensity of workers, increase of the working efficiency, improvement of the surface cleanliness of the cells, and improvement of the labor safety.
Owner:湖州鑫远电池系统技术有限公司
Sample pretreatment method based on flow combination ICP-MS (Inductively Coupled Plasma Mass Spectrometry) single cell protein detection
ActiveCN107314965AImpact AnalysisImpact statementMaterial analysis by electric/magnetic meansIndividual particle analysisPretreatment methodPeripheral blood mononuclear cell
The invention discloses a sample pretreatment method based on flow combination ICP-MS (Inductively Coupled Plasma Mass Spectrometry) single cell protein detection, and belongs to the technical field of sample pretreatment of flow cytometry. The sample pretreatment method comprises the following steps: (1) collecting and transporting a whole blood sample; (2) performing PBMC (Peripheral Blood Mononuclear Cell) cell separation; (3) performing cell active dyeing; (4) performing cell stimulation; (5) performing cell immobilization; (6) performing surface antibody dyeing; (7) performing intracellular phosphorylated protein dyeing; (8) performing single cell labeling, and the like. With the combination of a metal element labeled antibody with cell surface antigen, labeled cells are mixed with beads as internal reference for standardization, dead cells and living cells are distinguished in the pretreatment process, then the situation that the testing result analysis and description are affected by too many dead cells is avoided, single cells are distinguished from cell dimmers, cell trimers or even cell multimers, and flow combination ICP-MS single cell protein detection requirements can be very well met.
Owner:马鞍山普梅森医学检验实验室有限公司
Fuel cell and method of manufacturing the same
InactiveUS20110287339A1Improve abilitiesExtended service lifeFinal product manufactureFuel cell auxillariesFuel cellsInsertion depth
A fuel cell includes a unit cell, a cell fixing member and a welding portion. The unit cell includes a first electrode layer, an electrolyte layer surrounding the first electrode layer, a second electrode layer surrounding the electrolyte layer while exposing an end portion of the electrolyte layer, and a coating layer formed by coating a mixture of ceramic and metal on the exposed end portion of the electrolyte layer. The cell fixing member includes a flow tube inserted into the unit cell, a fixing tube provided to an outside of the flow tube, and a connecting portion connecting the fixing tube and the flow tube to each other and to restrict an insertion depth of the electrolyte layer and the first electrode layer. The welding portion fixes and seals the coating layer and the inner circumferential surface of the fixing tube to each other.
Owner:SAMSUNG SDI CO LTD
Carriers for Enzyme or Cell Immobilization and Immobilization Method Using the Carriers
ActiveUS20100028971A1Large specific surface areaLess fragileOrganic chemistryMicrobiological testing/measurementFlocculationHigh specific activity
The invention discloses a carrier made from an organic foam having open pores for enzymes or cells immobilization and the methods for preparing immobilized enzymes or cells. The invention uses flocculation and crosslinking technology to immobilize enzyme protein or cells on the organic foam material having open pores. The resultant immobilized products have larger specific surface area, higher specific activity and can be made into various shapes.
Owner:BIORIGHT WORLDWIDE
Cryopreservation of Biological Cells and Tissues
ActiveUS20150011000A1Bioreactor/fermenter combinationsBiological substance pretreatmentsBiological cellVitrification
The method involves placing an oocyte cell in a cell holder (1), securing the cell holder in a treatment station, applying a treatment solution to the cell by washing the cell with the solution, and rapidly cooling the cell holder and cell to a predetermined cryopreservation temperature for cryopreservation of the cell. The cell is cooled at a high rate sufficient to permit vitrification of the cell and any surrounding treatment solution to occur. The cell is then maintained at or below a predetermined storage temperature for storage. The method allows multiple cells to be treated simultaneously each secured within a respective cell holder.
Owner:GENEA IP HLDG PTY LTD
Rechargeable battery
InactiveUS20090111004A1Improve welding strengthSmall-sized cells cases/jacketsLarge-sized cells cases/jacketsRechargeable cellPrinted circuit board
A rechargeable battery includes: a bare cell; a protection circuit board installed on an upper surface of the bare cell; and a lead plate to connect the protection circuit board with the bare cell, wherein the lead plate includes a substrate fixing part fixed to the protection circuit board, a bare cell fixing part fixed to the bare cell, and a connection part to connect the substrate fixing part with the bare cell fixing part, the lead plate being installed such that the connection part is disposed between the protection circuit board and the upper surface of the bare cell. A welding strength is improved when the lead plate connecting the bare cell with the protection circuit board is installed, thereby prominently reducing defects in manufacturing packs and modules.
Owner:SAMSUNG SDI CO LTD
Cell inclusions analysis method based on microfluid chip
InactiveCN1734265AShort fusion timeFully contactedMaterial analysis by optical meansDecompositionCell substance
This invention provides an analytic method of inner micro-fluidic chip cell substance, which is characterized by the following: introducing biotin and affinity system; fixing the cell at a specific position of microchannel, which is decomposed by chemical reagent; making biotin- affinity protein micro-adsorption mode on the surface of microchannel; utilizing the special interaction of biotin and affinity; fitting biotin cell on the specific position of microchannel surface; decomposing the specific cell in the chemical buffer solution with high voltage; detecting the inner discharging substance by electrophoresis separation. This method realizes single cell sampling, operating, decomposing, separating and detecting by simple device, which is convenient and feasible for micro-fluidic chip cell analysis and decomposition.
Owner:DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
Kit for detecting human regulatory T cell subtypes and detection method
ActiveCN108508196AEasy to get ingredientsPreparing sample for investigationBiological material analysisRegulatory T cellPeripheral blood mononuclear cell
The invention provides a kit for detecting human regulatory T cell subtypes and a detection method and belongs to the technical field of cell subtype detection. The provided kit comprises components as follows: a blood diluent, a mononuclear cell separation medium, a cell culture fluid, a lymphocyte activation solution, dead cell removal dyes, an FcR blocking agent, a fluorescence labeled antibodyresisting human cell surface labeling molecules, a fluorescence labeled antibody resisting human intracellular molecules, a PBS buffer solution, lipopolysaccharide, a washing buffer solution, a celldyeing buffer solution, a cell fixing solution and a permeable membrane lotion. During detection, firstly, mononuclear cells in whole blood are separated, then, mononuclear cells of human peripheral blood are stimulated, finally, a fluorescent antibody is stained, and the regulatory T cell subtypes can be detected. By use of the kit and the detection method, 2-6 regulatory T cell subtypes can be simply and rapidly distinguished.
Owner:沈阳汇敏源生物科技有限责任公司
Cell selection apparatus, and cell selection method using the same
InactiveCN102066547AEasy to filterHigh Fusion Regeneration ProbabilityBioreactor/fermenter combinationsBiological substance pretreatmentsCell selectionBinding force
Owner:TOSOH CORP
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