165 results about "Cell fixing" patented technology

PCT No. PCT/FR97/00794 Sec. 371 Date May 15, 1998 Sec. 102(e) Date May 15, 1998 PCT Filed May 5, 1997 PCT Pub. No. WO97/42503 PCT Pub. Date Nov. 13, 1997A process for magnetic immunoseparation of cells, in particular bacteria, fetal cells, stock cells of bone marrow and circulating cancerous cells, of the type consisting in affixing the target cells on paramagnetic balls and in causing a magnetic field to act on a sample containing the affixed cells, the free cells and the surplus paramagnetic balls, in order to isolate the paramagnetic balls, in that the sample is caused to circulate in a tube the section of which is much less than the length over which the magnetic field is applied.

The invention particularly relates to a circulating tumor cell detection kit, a preparing method thereof and an application thereof. The circulating tumor cell detection kit comprises a kit body, detection reagents and a micro-fluidic chip, wherein the detection reagents and the micro-fluidic chip are arranged in the kit body. The micro-fluidic chip is formed by a PDMS substrate and a glass slide in an attached mode. A micro-channel is distributed on the surface of the PDMS substrate, and a fishbone-shaped alternate type mixing structure is arranged on the bottom face of the micro-channel. After the PDMS substrate and the glass slide are bonded, a microfluid channel is formed on the surface of the micro-channel and the surface of the glass slide, and capturing probes are loaded on the surface of the micro-channel. The invention further provides the preparing method of the micro-fluidic chip and a method for detecting circulating tumor cells through the kit. Compared with the prior art, the circulating tumor cell detection kit and the methods have the advantages that the capture probes are loaded on all the surfaces of the micro-channel, and therefore the capturing efficiency is improved; as the micro-fluidic chip is modified through single strand nucleotide, the storage time of the chip is prolonged; in addition, a cell-fixing-free straining method is provided, and therefore convenience is provided for further cell gene sequencing.

A capacitor module is provided with a plurality of capacitor cells each having a capacitor and a capacitor case provided with a first closed-bottomed screw hole with an opening on a bottom surface for housing the capacitor, a metallic cell-fixing body having a through-hole in communication with the first screw hole to which the capacitor cells are fixed by inserting a cell screw in the through-hole and the first screw hole, an insulator made of a thermally conductive insulating material and provided between the capacitor cells and the cell-fixing body for insulating the capacitor cells from the cell fixing body, and a metallic heat-dissipating body having a second closed-bottomed screw hole in which a cell-fixing body screw is inserted and having a flow passage for causing a cooling medium to flow on a rear surface side of a surface on which the second screw hole is provided.

This invention relates to a method for immobilizing cells under laminar flow conditions in a matrix formed by biocompatible charged polymers, to a flow device for the use with the method of the present invention, and to uses of the polymer matrices containing the cells immobilized using the method of the present invention.

The invention discloses liquid-base cell preservation liquid as well as a preparation method and application thereof, relating to the technical field of biology. The liquid-base cell preservation liquid contains the following components in parts by weight: 12-18 parts of a fixing agent, 2-8 parts of an anti-coagulation agent, 2-10 parts of a buffer agent, 0.5-0.8 part of an ion strength maintaining agent, 2-8 parts of an antimicrobial reagent, 0.05-0.2 part of a mucus dissolving agent and 0.1-0.4 part of a cleanser. The liquid-base cell preservation liquid has the advantages that the cell stability is high, cells are well fixed, the cost is low, the preserved cell forms are relatively complete, and the like. Besides, the invention further provides the preparation method of the liquid-basecell preservation liquid and application of the liquid-base cell preservation liquid in human body cast-off cells.

A pressure vessel for holding a pressurized fluid such as compressed natural gas (“CNG”) includes two end cells and zero or more interior cells. The cell geometry ensures that the cells meet one another at tangential circular surfaces, thereby reducing the tendency of adjacent cells to peel apart. A web secured about the cells includes two sheets that are tangent to the cells. Unused volumes between the cells and the web contain wedges of foam or rubber. A valve provides fluid communication between the interior of the pressure vessel and a pressurized fluid line. The filled weight of one pressure vessel does not exceed the filled weight of a conventional gasoline tank that occupies substantially the same space as the pressure vessel. The pressure vessel may be configured with exterior recesses for engaging conventional gasoline tank straps.

The invention relates to a cell fixative that can be used in the preparation of slides for investigation by microscopy techniques. In particular the present invention provides a cell fixative that contains a light curable mounting medium.

A liquid-based cell preservation liquid composite is characterized by comprising the following components in percentage by weight: cell fixing agent: 2-15% of formaldehyde, preservative: 0.1-5% of sodium diacetate, anticoagulant: 2-10% of sodium citrate, buffering agent: 0.5-2% of sodium chloride, chelating agent: 0.002-0.5% of ethylene diamine tetraacetic acid (EDTA), hemolytic agent: 0.5-10% ofglacial acetic acid, and water: 5-80% of pure water, wherein the formaldehyde is the analytical reagent, and accounts for 37-40%; the sodium diacetate is the food additives; the sodium citrate and the sodium chloride are analytical reagents, and NaCl is more than 99.5%; and the EDTA and the glacial acetic acid are analytical reagents, and H2COOH is more than 99.5%. The invention has the advantages that the liquid-based cell preservation liquid composite can keep the cell form to the maximum extent, can improve the diagnosis accuracy and can prevent the cell form of a specimen from significantdifference at room temperature for at least three months.

The invention provides a micro-fluidic chip used for cell immobilization and solution dilution. The invention comprises a transparent hard substrate layer one surface of which is provided with a plurality of microchannels by means of machining, and also comprises a transparent elastic rubber layer one surface of which is provided with micro-water pools by means of machining, wherein, the one surface of the transparent hard substrate layer which is provided with the microchannels by means of machining closely clings to the one surface of the transparent elastic rubber layer which is provided with the micro-water pools by means of micro-machining; the microchannels are overlapped with the micro-water pools to form a closed cell suspension or a solution flowing path; the dimension of the prior micro-water pools is changed through selection of different structures of the micro-water pools or through external force, thereby the flow condition of the whole chip can be controlled and cell immobilization of solution dilution are realized. The micro-fluidic chip can be used on a microchip to immobilize different biological living cells or abiological particles and then realize bioanalysis by taking the cells and molecules as the basis; simultaneously, the micro-fluidic chip can control different types of solution and mix the solution according to the set proportion and the diffusion conditions, and then the solution concentration or the concentration gradient required is obtained; and the micro-fluidic chip is used for high-flux biological sample analysis.

An apparatus for electrically contacting biological cells suspended in a liquid has a substrate having at least one opening and an electrode for electrically contacting a cell immobilized above the opening. A contact unit with a contact tip is arranged below said opening. A top end of the contact tip projects into the opening in such a way that it comes to bear against a cell membrane of the immobilized cell. The contact tip has a contact channel which ends at its top end. A hydrodynamic low pressure can be exerted upon the cell membrane via the contact channel, and the electrode is electrically connected to the contact channel. In a method for electrically contacting biological cells suspended in a liquid, a cell is immobilized above an opening provided in a substrate, and the immobilized cell is contacted via at least one electrode. For contacting the immobilized cell, a hydrodynamic low pressure is generated acting on a cell membrane through a contact tip projecting into the opening.

The invention discloses a urine preservation reagent and an application thereof. The urine preservation reagent comprises a nucleic acid buffer, a metal ion chelating agent, an antiseptic, a cell fixing agent, and a formaldehyde quenching agent; a nucleic acid buffer is Tris-HCl, a working pH value is 7-8, the metal ion chelating agent is EDTA, the antiseptic is citric acid and/or sodium citrate,the cell fixing agent is selected from at least one of paraformaldehyde, diazolidinyl urea, imidazolidinyl urea, and diethyl urea; and the formaldehyde quenching agent is selected from at least one ofglycine, lysine, ethene diamine and arginine. The urine preservation reagent can effectively preserve free DNA in urine, degradation can be avoided, and the urine preservation reagent establishes a base for subsequently detecting the free DNA; fragmentation of cells in the urine is prevented, a genome DNA background is reduced, and free DNA detection result accuracy is guaranteed. The urine preservation reagent is the novel reagent for preserving a urine sample, and provides the guarantee for clinical detection of the urine free DNA.

The invention relates to a cell modification device, comprising a centrifugation chamber with at least one cell modifying surface with a normal vector having an angle of 135-45° to the rotational axis of the centrifugation chamber, wherein the centrifugation chamber comprises at least one input/output port and the cells to be modified are immobilized at the cell modifying surfaces by the rotation of the centrifugation chamber at 2 to 2000 g. Furthermore, the invention relates to a method for modifying cells comprising the steps—introducing cells in a cell modification device, comprising a centrifugation chamber with at least one cell modifying surface with a normal vector having an angle of 135-45° to the rotational axis of the centrifugation chamber wherein and comprising at least one input/output port, —immobilizing the cells on the cell modifying surfaces by the rotation of the centrifugation chamber at 2 to 2000 g—maintaining the rotation of the rotation of the centrifugation chamber until the cells are modified.

The invention relates to a blotting material used for specific recognition of cells, and preparation and applications thereof. According to the blotting material, cells are taken as a template, cell culturing and cell immobilization technology are adopted to immobilize the cells on the surface of a substrate material, curing of a pre-polymerization system is carried out on the surface of the substrate material, the substrate material is removed via separation, and the template cells on the surface of an obtained cured polymer are removed so as to obtain a finished product. The blotting material is high in selectivity and stability, and can be used in identification, capturing, and enriching of cells.

It is intended to provide a novel method of immobilizing a protein and a protein chip, by which the protein can be immobilized at a high reproducibility while preventing the protein from inactivation without resort to a large-scaled apparatus and the protein can be immobilized even in a microchannel. Further, by using a cell adhesive protein as the protein to be immobilized, it is also possible to use a cell as a target and to provide a method of immobilizing a cell and a cell chip, by which a cell can be immobilized in an arbitrary region on a substrate.

The invention relates to a complete gluing apparatus and a gluing technology of a new energy automobile assembled battery. The apparatus comprises a main frame (1), the main frame (1) is provided with a conveying device (2), a feeding device (3), a gluing device (4) and a cell grasping and stacking device (5) are sequentially arranged above the conveying device (2), the main frame (1) is provided with a cell overturning device (6), the conveying device (2) is provided with a positioning sensor (7), and the positioning sensor (7) is connected with a controller (8); the cell grasping and stacking device grasps cells to the cell overturning device; and the cell overturning device overturns the cells to a cell fixing and clamping assembly, the cells are conveyed to the gluing device through a conveying track and are glued, and the cell grasping and stacking device grasps and stacks the cells. The apparatus and the technology have the characteristics of improvement of the gluing quality, reduction of the labor intensity of workers, increase of the working efficiency, improvement of the surface cleanliness of the cells, and improvement of the labor safety.

The invention discloses a sample pretreatment method based on flow combination ICP-MS (Inductively Coupled Plasma Mass Spectrometry) single cell protein detection, and belongs to the technical field of sample pretreatment of flow cytometry. The sample pretreatment method comprises the following steps: (1) collecting and transporting a whole blood sample; (2) performing PBMC (Peripheral Blood Mononuclear Cell) cell separation; (3) performing cell active dyeing; (4) performing cell stimulation; (5) performing cell immobilization; (6) performing surface antibody dyeing; (7) performing intracellular phosphorylated protein dyeing; (8) performing single cell labeling, and the like. With the combination of a metal element labeled antibody with cell surface antigen, labeled cells are mixed with beads as internal reference for standardization, dead cells and living cells are distinguished in the pretreatment process, then the situation that the testing result analysis and description are affected by too many dead cells is avoided, single cells are distinguished from cell dimmers, cell trimers or even cell multimers, and flow combination ICP-MS single cell protein detection requirements can be very well met.

The invention discloses a carrier made from an organic foam having open pores for enzymes or cells immobilization and the methods for preparing immobilized enzymes or cells. The invention uses flocculation and crosslinking technology to immobilize enzyme protein or cells on the organic foam material having open pores. The resultant immobilized products have larger specific surface area, higher specific activity and can be made into various shapes.

The method involves placing an oocyte cell in a cell holder (1), securing the cell holder in a treatment station, applying a treatment solution to the cell by washing the cell with the solution, and rapidly cooling the cell holder and cell to a predetermined cryopreservation temperature for cryopreservation of the cell. The cell is cooled at a high rate sufficient to permit vitrification of the cell and any surrounding treatment solution to occur. The cell is then maintained at or below a predetermined storage temperature for storage. The method allows multiple cells to be treated simultaneously each secured within a respective cell holder.

The invention provides a kit for detecting human regulatory T cell subtypes and a detection method and belongs to the technical field of cell subtype detection. The provided kit comprises components as follows: a blood diluent, a mononuclear cell separation medium, a cell culture fluid, a lymphocyte activation solution, dead cell removal dyes, an FcR blocking agent, a fluorescence labeled antibodyresisting human cell surface labeling molecules, a fluorescence labeled antibody resisting human intracellular molecules, a PBS buffer solution, lipopolysaccharide, a washing buffer solution, a celldyeing buffer solution, a cell fixing solution and a permeable membrane lotion. During detection, firstly, mononuclear cells in whole blood are separated, then, mononuclear cells of human peripheral blood are stimulated, finally, a fluorescent antibody is stained, and the regulatory T cell subtypes can be detected. By use of the kit and the detection method, 2-6 regulatory T cell subtypes can be simply and rapidly distinguished.