85 results about "Bovine blood" patented technology

A sterile method for preparing stable thrombin component from a single donor's plasma in which the thrombin component and the clotting and adhesive proteins component are harvested simultaneously from the same donor plasma in less than one hour. The combined components provide an improved biological hemostatic agent and tissue sealant by virtue of its freedom from the risk of contaminating viruses or bacteria from allogenic human or bovine blood sources. The thrombin provides polymerization of the clotting and adhesive proteins in less than five seconds, and is sufficiently stable to provide that fast clotting over a six hour period. Further, the clotting times can be predictably lengthened by diluting the thrombin with saline.

A sterile method for preparing stable thrombin component from a single donor's plasma in which the thrombin component is harvested simultaneously from the clotting and adhesive proteins component from the same donor plasma in less than one hour. The combined components provide an improved biological hemostatic agent and tissue sealant by virtue of its freedom from the risk of contaminating viruses or bacteria from allogenic human or bovine blood sources. The thrombin provides polymerization of the clotting and adhesive proteins in less than five seconds, and is sufficiently stable to provide that fast clotting over a six hour period. Further, the clotting times can be predictably lengthened by diluting the thrombin with saline.

A sterile method for preparing stable thrombin component from a single donor's plasma in which the thrombin component is harvested simultaneously from the clotting and adhesive proteins component from the same donor plasma in less than one hour. The combined components provide an improved biological hemostatic agent and tissue sealant by virtue of its freedom from the risk of contaminating viruses or bacteria from allogenic human or bovine blood sources. The thrombin provides polymerization of the clotting and adhesive proteins in less than five seconds, and is sufficiently stable to provide that fast clotting over a six hour period. Further, the clotting times can be predictably lengthened by diluting the thrombin with saline.

The invention relates to an application of an earthworm fibrinolytic enzyme in preparation of liver fibrosis resistant medicine or liver protection health-care food, which belongs to the technical field of biology. The effective dose of an oral liver fibrosis resistant earthworm fibrinolytic enzyme is 1,000-3,000 urokinase unit / person / day. Proved by experiments, earthworm collagenase can degrade collagen but cannot degrade (ox blood) fibrin, and the earthworm fibrinolytic enzyme can degrade the (ox blood) fibrin and can also degrade the collagen, thus the activity of the earthworm fibrinolytic enzyme is higher than the activity of the earthworm collagenase and the activity of a collagenase standard product (Sigma I type). Proved by a liver fibrosis resistant experiment by using a rat, the oral liver fibrosis resistant earthworm fibrinolytic enzyme has obvious effect and no obvious poisonous and side effect, but the oral liver fibrosis resistant earthworm collagenase does not have obvious effect.

The invention discloses a determination method for heparin activity. The method comprises three steps of treatment of standard substances and samples, establishment of a standard curve and sample measurement, and calculation of heparin activity contents in the samples, wherein reaction systems and time are as follows: (1) 10-40 microliters standard curve establishment solution and samples with 0-0.1 IU / ml heparin activity concentration are mixed with 10-40 microliters antithrombase with 1 IU / ml concentration, and are incubated for 3 minutes at the temperature of 37 DEG C; (2) 10-40 microliters activated bovine blood coagulation factor X with 10 nkat / ml concentration is added for mixing, and is incubated for 1.5 minutes at the temperature of 37 DEG C; (3) 10-40 ml color development substrate S-2765 with 2.5 mmol / L concentration is added for mixing, and is incubated for 3 minutes at the temperature of 37 DEG C; and (4) 10-40 microliters acetic acid with 50% of volume concentration is added for evenly mixing, and then the reaction is stopped. The method has the advantages of lower reagent dosage, high detection efficiency and high precision of measurement results, and is more suitable for detecting the samples with lower heparin activity.

The invention relates to a screening method for a hybridoma cell line able to secrete an anti-bovine immunoglobulin IgG monoclonal antibody for bovine immunoglobulin IgG detection, the hybridoma cell line, the monoclonal antibody secreted thereby and application in the field of bovine immunoglobulin IgG detection. The hybridoma cell is preserved in China Center For Type Culture Collection, Wuhan University at Luojiashan, Wuchang, Wuhan City in Hubei Province, and the preservation number is CCTCC No:2013183. The anti-bovine immunoglobulin IgG monoclonal antibody secreted by the hybridoma cell has the advantages of strong specificity, large affinity and high titer and the like, and can be widely applied in the detection reagent or detection equipment field of bovine immunoglobulin IgG. A bovine immunoglobulin IgG rapid detection card built based on the immunochromatography principle can be used for detection of bovine immunoglobulin IgG in colostrum and its products, bovine blood and other samples, and has significant advantages compared with conventional detection methods in the aspects of specificity, sensitivity and detection efficiency, etc.

The invention relates to the technical field of a biomedical nano material, and more particularly to a tumor microenvironment responsive diagnosis and treatment integrated nano probe and a preparationmethod thereof. The nano probe uses bovine serum albumin as a template and a sulfur source to construct nanoparticles having uniform dispersion and uniform particle size with Bi2S3 and MnO2 by a biomineralization mode, and an activated carboxyl group of bovine serum albumin is cross-linked with an amino group of a peptide chain to link a matrix metalloproteinase-responsive peptide chain to provide the nano probe with multimodal imaging and treatment. The material is subjected to matrix metalloproteinases in the tumor microenvironment, and the peptide chain is sheared to promote the hydrophobic chain exposure, which results increase in the hydrophobic properties of the nanoparticles, the degree of enrichment of the material in the tumor site is enhanced, which can effectively improve the imaging and therapeutic performance of the nano probe.

The invention discloses a pig blood protein-free extract preparation, pertaining to the technical field of biochemical drugs. The pig blood protein-free extract freeze-dried powder injection preparation is prepared by pig blood protein-free extract and an excipient, and the weight ratio of the pig blood extract and the excipient is 200: 30 to 500. The pig blood protein-free extract of the invention applies pig blood as a raw material source to replace calf blood in the preparation of drugs for the treatment of cerebral vascular diseases, the improvement of nervous function defects caused by cerebral ischemia and craniocerebral trauma and the treatment of peripheral blood disorder symptoms, thus producing the biochemical drugs with the same function, replacing the similar products with the calf blood source, becoming the best choice of drug for the treatment and the auxiliary treatment of cardiovascular and cerebrovascular diseases and having very wide range of clinical application prospect. The product of the invention adopts the pig blood as the raw material, compared with the similar calf blood products, the product has the product advantages of high safety and high biological activity; furthermore, as the raw material is easy to obtain, the cost is low, the production process is advanced, the period is short and the yield is high, the product of the invention has the price advantage compared with the similar calf blood products.

An immobilized cholinesterase and preparation and application thereof are disclosed, the designated carrier is water-soluble material such as polyvinyl alcohol, polyvinyl pyrrolidone or Arabic gum, capable of film-forming by drying and of being dissolved rapidly in aqueous solution; the carrier also comprises composite material formed by the water-soluble material and lipoplast. 1-10mg / mL of bovine serum albumin phosphate buffer solution is used as a protective agent; the water-soluble material is used to immobilize cholinesterase, cholinesterase water-soluble material solution is either directly adhered to solid material by drip or soakage and then dried to form a thin film or dripped into a mould and then dried to form enzyme granules, when the material is put into the solution, enzyme molecules therein are dissolved and released immediately to catalytically function in homogeneous phase with better catalytic efficiency and inhibitive sensitivity; the invention can be applied to rapid detection of pesticide residue or screening of cholinesterase inhibitor.

The invention relates to a human peripheral blood lymphocytes culture medium and the application thereof. The human peripheral blood lymphocytes culture medium of the invention comprises: RPMI1640 liquid culture medium 77-81.9%, bovine blood 10-14.9%, PHA 4-9%, non-sulfated glycosaminoglycan 2-5%, CPPs 2-5% and Double-resist 0.05-0.1%. The culture medium provided in the invention achieves a high lymphocyte conversion rate, a high lymphocyte split index and a good reagent stability; can obtain a human chromosome figure which can provide a firm basis to chromosome core type analysis and clinic diagnosis.

The present invention relates to a gamma-interferon sandwich ELISA detection method based on recombinant fusion antigen protein, further relates to a recombinant fusion antigen protein preparation method and a gamma-interferon monoclonal antibody preparation method, and belongs to the technical field of medical examination determination. According to the present invention, the recombinant fusion antigen protein is formed by linking secretory antigen protein MPB70, antigen protein CFP10 and antigen protein ESAT6 through peptide bonds, the gamma-interferon monoclonal antibody is prepared through immunization of animals, cell fusion, hybridoma cell screening, cloning and purification, and the recombinant fusion antigen protein and the gamma-interferon monoclonal antibody are adopted to establish a sandwich ELISA detection method; and the recombinant fusion antigen protein can rapidly stimulate a bovine blood sample to secret gamma-interferon, and the gamma-interferon monoclonal antibody can quickly and specifically identify bovine gamma-interferon, such that strong sensitivity and strong specificity are provided, and broad application prospects are provided in the field of buffalo tuberculosis diagnosis.

The invention discloses a method for preparing a cored meatball by making use of beef processing by-products. According to the method, beef tendon pulp material which is rich in bone nutrition is used as the outer base of a stuffed meatball and boneless and minced beef, bovine liver and bovine blood meal are made into internal meatball stuffing. The method comprises the steps of (1) preparation of outer pulp material; (2) preparation of internal meatball; and (3) preparation of stuffed meatballs. The stuffed meatball prepared by the method provided by the invention has the advantages that the stuffed meatball is high in nutrition value and has multiple layers of flavors in taste; the outer layer of the meatball is sparkling, transparent, fine, smooth and flexible; the internal stuffing is tender, tasty and refreshing, the taste is coordinative, the flavor is unique, and no prominent fishy smell exists. The product prepared by the method provided by the invention improves the utilization value and additional value of beef processing by-products, and enriches the types of deep and fine processing food of beef by-products. And moreover, the raw materials of the product are natural food materials, no food additives such as edible gum and composite phosphate which are used in traditional meatballs are used; thus, the product well cater to the natural and healthy food ideal of modern people.

The invention relates to the field of biotechnologies, and especially relates to a leech-specific nutritional induction solution, and a method for extracting natural hirudin. The leech-specific nutritional induction solution includes sodium chloride, compound amino acids, glucose, trans-4,5-epoxy-(E)-2-decenal and sterile water. A technology for satiating Poecilobdella manillensis Lesson by usingliving body induction is adopted to avoid large-scale killing of leeches and make the leeches reused. Feeding of the specific nutritional induction solution instead of whole bovine blood and other induction substances with poor palatability can induce the full intake of leeches and reduce the mixing of a large amount of blood and other impurities, so the subsequent separation and purification of natural hirudin are benefited.

A serum alternative and serum-free rainbow trout embryo cell line medium relates to a serum alternative and a serum-free embryo cell line medium. The invention solves the problems of too high cost and unstable quality of the serum used in the medium, and the present lack of suitable rainbow trout serum-free embryo cell line medium. The serum alternative consists of 0.3g rhIGF-1, 0.5g rh-bFGF, 0.65g PDGF-B, 1.5g bovine serum albumin, 5g bovine transferring, 34g Beta- mercaptoethanol, 0.294g glutamatic acid, 0.110g sodium pyruvate, and 2 to 3g nonessential amino acid. The medium consists of RPMI-1640 medium and the serum alternate. The serum alternate of the invention has price as low as 60 percent of that of bovine serum and stable quality. The rainbow trout embryo cell line medium cultured by the serum-free rainbow trout embryo cell line medium of the invention has the advantages of rapid breeding speed and long regenerating cycle.

The invention discloses a detection method by using a PRSS2 gene as a lactoprotein content molecular marker. The method comprises the following steps: 1. carrying out whole genome DNA (deoxyribonucleic acid) extraction on bovine blood, and inspecting the DNA quality by agarose gel electrophoresis; 2. carrying out PCR (polymerase chain reaction) amplification on the bovine PRSS2 gene segment by using the whole genome DNA as a template and a primer A as the primer; 3. carrying out mixed-cell PCR on the whole genome DNA to detect the polymorphism sites; and 4. carrying out sequencing on an individual sample, and carrying out polymorphism correlation analysis according to the sequencing result. The method is utilized to detect the relevance between 5 SNP (single-nucleotide polymorphism) sites and lactoprotein traits in the bovine milk quality. The invention provides a simple quick genetic marker for screening and detecting close relevance to bovine milk quality traits on the DNA level, and the genetic marker can be used for bovine molecular breeding. The method solves the problem of high complexity in the SSCP (single strand conformation polymorphism) and PCR-PFLP (polymerase chain reaction-restriction fragment length polymorphism) techniques, and enhances the accuracy and precision.

The invention belongs to the field of food processing and discloses a cake prepared from a substitute for partially replacing eggs. The cake is prepared from fresh bovine plasma or / and bovine plasma protein powder which is used as an egg substitute for partially replacing the eggs. The sum of the weights of the eggs and the egg substitute accounts for 30 to 40 percent of the total weight of the raw materials of the prepared cake. The cake comprises the following other raw materials in percentage by weight: 15 to 25 percent of flour, 15 to 25 percent of sugar, 0.20 to 0.25 percent of salt, 5 to 10 percent of cake oil, 0.20 to 0.30 percent of baking powder, 2 to 3 percent of milk powder, 5 to 15 percent of water and 2 to 3 percent of salad oil. In the method, the fresh bovine plasma or / and the bovine plasma protein powder in a certain proportion are used for partially replacing the eggs to produce the cake, a new way of utilizing bovine blood is opened up, the pollution of the bovine blood to the environment is reduced, the cost of the raw materials of the cake is reduced, and the quality of the cake is improved.

The invention provides a method for optimally isolating monocytes in bovine peripheral blood. ACD-A is taken as an anticoagulant, the method comprises the optimization of centrifugal conditions, the optimization of cell cleaning conditions, the optimization of wall-sticking conditions and the like, and the success rate of the isolation is greatly improved. In the method, a whole set of complete system which comprises the sampling of bovine blood, the isolation of the monocytes in the bovine peripheral blood and the final transformation of the monocytes into macrophages is established for the first time, and the counting and dyeing are not required in the process, so that the time is saved and an isolation effect is improved. The survival rate of the monocytes isolated by the method is high; and the method is high in experimental repeatability and strong in operability, and provides powerful technical support for simply, conveniently and efficiently isolating the monocytes in the bovine peripheral blood.

The invention relates to a human peripheral blood lymphocytes culture medium and the application thereof. The human peripheral blood lymphocytes culture medium of the invention comprises: RPMI1640 liquid culture medium 77-81.9%, bovine blood 10-14.9%, PHA 4-9%, non-sulfated glycosaminoglycan 2-5%, CPPs 2-5% and Double-resist 0.05-0.1%. The culture medium provided in the invention achieves a high lymphocyte conversion rate, a high lymphocyte split index and a good reagent stability; can obtain a human chromosome figure which can provide a firm basis to chromosome core type analysis and clinic diagnosis.

The invention discloses a method for extracting albumin and globulin from bovine serum and a method for utilizing ox blood and relates to the technical field of protein extraction. According to the method for extracting albumin and globulin from bovine serum provided by the invention, the principle of plasma protein depositing in saline solution in a certain saturability is utilized, (NH4)2SO4 fractional precipitation is adopted and the bovine serum albumin is further purified. The bovine serum albumin is high in purity, is low in endotoxin content and meets the requirement of Chinese Biological Product Regulation. A saturated (NH4)2SO4 fractional repeated salting out method is utilized to extract the bovine serum albumin; the high molecular weight plasma protein is extremely removed; and the purity of the extracted bovine serum albumin is high. The method for extracting albumin and globulin from bovine serum provided by the invention is simple in process; no special device is required; the method is suitable for expanded production; and the method for utilizing ox blood provided by the invention can increase the use ratio of the ox blood.

The invention discloses a process for extracting bovine serum albumin through thermal alcohol method comprising the steps of, separating plasma, charging sodium citrate into ox blood, centrifugal extracting, placing the collected plasma into laminated reaction tank, heating and charging in sodium octoate, agitating slowly, the temperature being 55-65 deg. C, adjusting pH to 4.0-6.5 by charging in hydrochloric acid, charging in alcohol, filtering, desalinizing and concentrating, freeze-drying to obtain the end product. The process requires less material by short process flow, it is suitable fo mass production.

The invention discloses a separation method of bovine blood CD4 + T lymphocytes, and relates to the field of bioengineering.The separation method comprises the following steps that oxtail vein blood is collected; separating lymphocytes by using a commercial lymphocyte separation kit; centrifuging, collecting precipitate, and adding a red blood cell lysis solution to lyse red blood cells; adding a culture medium, washing, centrifuging and collecting precipitate; after cell counting, adding a primary antibody of CD4 + T lymphocytes for incubation; adding a magnetic bead secondary antibody for incubation; and finally, separating the CD4 + T lymphocytes by using a magnetic column. The invention introduces the bovine CD4 + T lymphocyte separation method for the first time, and fills the blank of the test method technology in the field. The bovine CD4 < + > T lymphocytes obtained by the method are good in activity, large in quantity and high in purity. The kit can be widely applied to clinical diagnosis besides scientific research, and has a good market prospect.

A pretreatment method for measuring lead in blood by graphite furnace atomic absorption process belongs to the field of a detection method of lead content in blood and especially relates to a pretreatment method for measuring content of lead in blood by graphite furnace atomic absorption process. The method comprises preparation of a matrix modifier and preparation of a lead standard solution. The matrix modifier contains ammonium biphosphate, Triton and nitric acid. The method comprises the following steps: Step 1, dissolving a freeze-dried bovine blood lead standard substance as required; Step 2, successively adding nitric acid, Triton and ammonium biphosphate, and stabilizing for 1-2 days; Step 3, successively adding whole blood, nitric acid and hydrogen peroxide into the matrix modifier so as to prepare a measuring sample; and Step 4, treating the measuring sample by water bath for 1-2 hours until the sample turns faint yellow from atropurpureus for measurement. The pretreatment method provides a method for accurately measuring content of lead in blood. The pretreatment method is simple to operate, has good stability and high precision, and eliminates interference of blood sample matrix composition.

The invention discloses fertilizer for production of high-Fe peaches. The fertilizer comprises 40-45 parts of kiwi fruit tree leaves, 30-35 parts of peach tree leaves, 20-25 parts of chicken blood, 20-25 parts of porcine blood, 15-20 parts of bovine blood, 30-35 parts of dried fowl manure, 15-20 parts of soybean cakes, 10-15 parts of plant ash, 5-7 parts of ferrous sulfate, 3-5 parts of an EM stock solution, 0.5-0.8 parts of molasses and 2-4 parts of a deodorant. The high-Fe peaches have the functions of invigorating qi and blood, nourishing yin and generating body fluid and can be applied to people with deficiency of Qi and blood, emaciation with sallow complexion, palpitation and short breath after serious illnesses; the high-Fe peaches have high Fe content and are ideal auxiliary food for patients with iron-deficiency anemia.

The present invention is related to immunoglobulin peptides that recognize a thermostable antigen from bovine blood. The invention also provides methods for determining the presence of bovine blood in a food sample or an animal feed sample.

Xenon based biosensors have the potential to detect and localize biomarkers associated with a wide variety of diseases. The development and nuclear magnetic resonance (NMR) characterization of cage molecules which encapsulate hyperpolarized xenon is imperative for the development of these xenon biosensors. We acquired 129Xe NMR spectra, and magnetic resonance images and a HyperCEST saturation map of cucurbit[6]uril (CB6) in whole bovine blood. We observed a mean HyperCEST depletion of 84% (n=5) at a concentration of 5 mM and 74% at 2.5 mM. Additionally, we collected these data using a pulsed HyperCEST saturation pre-pulse train with a SAR of 0.025 W / kg which will minimize any potential RF heating in animal or human tissue.

The invention discloses a smoked sausage. The smoke sausage comprises the following raw materials in parts by weight: 40 to 60 parts of duck breast meat, 25 to 28 parts of beef, 1 to 3 parts of garlic, 1 to 3 parts of ginger, 1 to 3 parts of edible salt, 1 to 3 parts of liquorice, 0.2 to 0.8 part of sodium hexametaphosphate and sodium tripolyphosphate, 0.5 to 1.5 parts of sodium citrate, 0.3 to 0.5 part of ground cinnamon, 4 to 6 parts of degreased milk powder, 0.2 to 0.5 part of allspice powder, 0.1 to 0.2 part of a sausage forming agent and 10 to 18 parts of salting liquor. The invention also discloses a processing process of the smoked sausage. The processing process comprises the steps of weighing raw materials, salting, mincing meat, chopping, filling a casing, smoking, steaming and packaging. Compared with a traditional process, for the processing process disclosed by the invention, the meat is preserved and salted through the salting liquor, and a finished product obtained by adopting a special cattle blood glutinous rice process is fresh and delicious in taste, reddish in appearance, moderate in brightness and not greasy.

The invention discloses a primer composition for detecting bovine citrullinemia harmful genes and a kit and an application thereof. The primer composition disclosed by the invention is composed of a primer set A and a primer set B, wherein the primer set A is composed of a primer 1 and a primer 2, and the primer set B is composed of a primer 3 and a primer 4; and the nucleotide sequences of the primer 1 to the primer 4 are respectively shown in SEQ ID NO.1-4. The invention also provides a kit containing the primer combination, a method for detecting bovine citrullinemia harmful genes by using the kit provided by the invention comprises the following steps: extracting the whole set of genomic DNA in bovine blood to serve as a template, carrying out nested PCR (Polymerase Chain Reaction) and sequencing the obtained PCR products. The base change of a mutation site can be directly controlled according to the sequencing result, thereby ensuring the result accuracy and meeting the requirements of detection technology with such characteristics as high speed, accuracy, high throughput and the like.

The invention discloses a traditional Chinese medicinal composition for treating chocolate cyst. The traditional Chinese medicinal composition is prepared from the following medicines by weight: 13g of Chinese angelica, 12g of cistanche deserticola, 6g of waxgourd seed, 11g of platycladi seed, 20g of cornu bubali, 10g of willow root, 15g of pork intestines, 20g of fresh bovine blood, 1g of dark plum, 200g of washing water of rice, 15g of ginger, 10g of diaphragma juglandis, 20g of lard oil, 30g of wheat bran. A preparation method comprises the following steps: wrapping 6g of waxgourd seed, 11g of platycladi seed, 20g of cornu bubali and 10g of willow root with the pork intestines, adding 200g of washing water of rice for decocting, keeping boiling for 20 minutes and filtering liquid medicine to obtain liquid medicine A; sequentially adding 13g of Chinese angelica and 10g of diaphragma juglandis into the liquid medicine A, decocting to boiling, and keeping boiling for 20 minutes; finally, simultaneously adding 12g of cistanche deserticola and 1g of dark plum, and filtering liquid medicine to obtain an oral medicine.

The invention relates to a composition for improving anemia as well as functional food, a preparation method and application thereof. The composition for improving anemia comprises the following raw materials in parts by weight: 5-25 parts of bovine bone marrow polypeptide powder, 10-30 parts of red date powder, 20-40 parts of bovine blood protein polypeptide powder, 12-25 parts of Chinese wolfberry fruit powder and 13-27 parts of fructo-oligosaccharide. According to the composition for improving anemia, starting from the reasons of absorption and utilization rate of iron in iron-deficiency anemia, recovery of hematopoietic function and the like, the aim of effectively adjusting and improving anemia conditions of anemia crowds can be achieved through mutual cooperation and synergistic effects of multiple components. The composition is high in effective rate and good in safety for anemia caused by iron deficiency in diet, excessive dieting or irregular diet, digestive tract function weakening, poor nutrient absorption, aplastic anemia and the like.

The invention belongs to the technical field of industrial labor protection, and particularly relates to an anti-splashing, easy-to-clean isolation and collection device in a beef cattle slaughteringworkshop. In the prior art, beef cattle need to be drained after being slaughtered, and devices are easy to cause splashing, and cannot meet the quantity requirement of blood draining. The device of the invention is provided with two blood draining tanks which are respectively used for receiving bovine blood and soiling liquid; an A-shaped isolation part is arranged above the side plate which is arranged between the two blood draining tanks; two sides of the isolation part are provided with anti-splashing parts; the isolation part and the anti-splashing parts are arranged in parallel along theblood draining tanks; the isolation part and the two anti-splashing parts respectively form a bovine blood channel and a soiling liquid channel; and the isolation part is provided with an operating rod which is assembled on an adjusting guide rail through a sliding block, so that the operating rod drives the isolation part to be adjusted left and right and / or up and down. According to the invention, the isolation part and the anti-splashing parts are adopted to form the two channels of the bovine blood channel and the soiling liquid channel so as to realize separate collection and treatment on the bovine blood and the soiling liquid; and the isolation part is provided with the adjusting guide rail which is capable of being adjusted horizontally and vertically and can adjust the hanging height of cattle.