83 results about "Bovine blood" patented technology

A sterile method for preparing stable thrombin component from a single donor's plasma in which the thrombin component and the clotting and adhesive proteins component are harvested simultaneously from the same donor plasma in less than one hour. The combined components provide an improved biological hemostatic agent and tissue sealant by virtue of its freedom from the risk of contaminating viruses or bacteria from allogenic human or bovine blood sources. The thrombin provides polymerization of the clotting and adhesive proteins in less than five seconds, and is sufficiently stable to provide that fast clotting over a six hour period. Further, the clotting times can be predictably lengthened by diluting the thrombin with saline.

The invention discloses a determination method for heparin activity. The method comprises three steps of treatment of standard substances and samples, establishment of a standard curve and sample measurement, and calculation of heparin activity contents in the samples, wherein reaction systems and time are as follows: (1) 10-40 microliters standard curve establishment solution and samples with 0-0.1 IU/ml heparin activity concentration are mixed with 10-40 microliters antithrombase with 1 IU/ml concentration, and are incubated for 3 minutes at the temperature of 37 DEG C; (2) 10-40 microliters activated bovine blood coagulation factor X with 10 nkat/ml concentration is added for mixing, and is incubated for 1.5 minutes at the temperature of 37 DEG C; (3) 10-40 ml color development substrate S-2765 with 2.5 mmol/L concentration is added for mixing, and is incubated for 3 minutes at the temperature of 37 DEG C; and (4) 10-40 microliters acetic acid with 50% of volume concentration is added for evenly mixing, and then the reaction is stopped. The method has the advantages of lower reagent dosage, high detection efficiency and high precision of measurement results, and is more suitable for detecting the samples with lower heparin activity.

The invention relates to a human peripheral blood lymphocytes culture medium and the application thereof. The human peripheral blood lymphocytes culture medium of the invention comprises: RPMI1640 liquid culture medium 77-81.9%, bovine blood 10-14.9%, PHA 4-9%, non-sulfated glycosaminoglycan 2-5%, CPPs 2-5% and Double-resist 0.05-0.1%. The culture medium provided in the invention achieves a high lymphocyte conversion rate, a high lymphocyte split index and a good reagent stability; can obtain a human chromosome figure which can provide a firm basis to chromosome core type analysis and clinic diagnosis.

The present invention relates to a gamma-interferon sandwich ELISA detection method based on recombinant fusion antigen protein, further relates to a recombinant fusion antigen protein preparation method and a gamma-interferon monoclonal antibody preparation method, and belongs to the technical field of medical examination determination. According to the present invention, the recombinant fusion antigen protein is formed by linking secretory antigen protein MPB70, antigen protein CFP10 and antigen protein ESAT6 through peptide bonds, the gamma-interferon monoclonal antibody is prepared through immunization of animals, cell fusion, hybridoma cell screening, cloning and purification, and the recombinant fusion antigen protein and the gamma-interferon monoclonal antibody are adopted to establish a sandwich ELISA detection method; and the recombinant fusion antigen protein can rapidly stimulate a bovine blood sample to secret gamma-interferon, and the gamma-interferon monoclonal antibody can quickly and specifically identify bovine gamma-interferon, such that strong sensitivity and strong specificity are provided, and broad application prospects are provided in the field of buffalo tuberculosis diagnosis.

The invention relates to the field of biotechnologies, and especially relates to a leech-specific nutritional induction solution, and a method for extracting natural hirudin. The leech-specific nutritional induction solution includes sodium chloride, compound amino acids, glucose, trans-4,5-epoxy-(E)-2-decenal and sterile water. A technology for satiating Poecilobdella manillensis Lesson by usingliving body induction is adopted to avoid large-scale killing of leeches and make the leeches reused. Feeding of the specific nutritional induction solution instead of whole bovine blood and other induction substances with poor palatability can induce the full intake of leeches and reduce the mixing of a large amount of blood and other impurities, so the subsequent separation and purification of natural hirudin are benefited.

A serum alternative and serum-free rainbow trout embryo cell line medium relates to a serum alternative and a serum-free embryo cell line medium. The invention solves the problems of too high cost and unstable quality of the serum used in the medium, and the present lack of suitable rainbow trout serum-free embryo cell line medium. The serum alternative consists of 0.3g rhIGF-1, 0.5g rh-bFGF, 0.65g PDGF-B, 1.5g bovine serum albumin, 5g bovine transferring, 34g Beta- mercaptoethanol, 0.294g glutamatic acid, 0.110g sodium pyruvate, and 2 to 3g nonessential amino acid. The medium consists of RPMI-1640 medium and the serum alternate. The serum alternate of the invention has price as low as 60 percent of that of bovine serum and stable quality. The rainbow trout embryo cell line medium cultured by the serum-free rainbow trout embryo cell line medium of the invention has the advantages of rapid breeding speed and long regenerating cycle.

The invention discloses a detection method by using a PRSS2 gene as a lactoprotein content molecular marker. The method comprises the following steps: 1. carrying out whole genome DNA (deoxyribonucleic acid) extraction on bovine blood, and inspecting the DNA quality by agarose gel electrophoresis; 2. carrying out PCR (polymerase chain reaction) amplification on the bovine PRSS2 gene segment by using the whole genome DNA as a template and a primer A as the primer; 3. carrying out mixed-cell PCR on the whole genome DNA to detect the polymorphism sites; and 4. carrying out sequencing on an individual sample, and carrying out polymorphism correlation analysis according to the sequencing result. The method is utilized to detect the relevance between 5 SNP (single-nucleotide polymorphism) sites and lactoprotein traits in the bovine milk quality. The invention provides a simple quick genetic marker for screening and detecting close relevance to bovine milk quality traits on the DNA level, and the genetic marker can be used for bovine molecular breeding. The method solves the problem of high complexity in the SSCP (single strand conformation polymorphism) and PCR-PFLP (polymerase chain reaction-restriction fragment length polymorphism) techniques, and enhances the accuracy and precision.

The invention belongs to the field of food processing and discloses a cake prepared from a substitute for partially replacing eggs. The cake is prepared from fresh bovine plasma or/and bovine plasma protein powder which is used as an egg substitute for partially replacing the eggs. The sum of the weights of the eggs and the egg substitute accounts for 30 to 40 percent of the total weight of the raw materials of the prepared cake. The cake comprises the following other raw materials in percentage by weight: 15 to 25 percent of flour, 15 to 25 percent of sugar, 0.20 to 0.25 percent of salt, 5 to 10 percent of cake oil, 0.20 to 0.30 percent of baking powder, 2 to 3 percent of milk powder, 5 to 15 percent of water and 2 to 3 percent of salad oil. In the method, the fresh bovine plasma or/and the bovine plasma protein powder in a certain proportion are used for partially replacing the eggs to produce the cake, a new way of utilizing bovine blood is opened up, the pollution of the bovine blood to the environment is reduced, the cost of the raw materials of the cake is reduced, and the quality of the cake is improved.

The invention provides a method for optimally isolating monocytes in bovine peripheral blood. ACD-A is taken as an anticoagulant, the method comprises the optimization of centrifugal conditions, the optimization of cell cleaning conditions, the optimization of wall-sticking conditions and the like, and the success rate of the isolation is greatly improved. In the method, a whole set of complete system which comprises the sampling of bovine blood, the isolation of the monocytes in the bovine peripheral blood and the final transformation of the monocytes into macrophages is established for the first time, and the counting and dyeing are not required in the process, so that the time is saved and an isolation effect is improved. The survival rate of the monocytes isolated by the method is high; and the method is high in experimental repeatability and strong in operability, and provides powerful technical support for simply, conveniently and efficiently isolating the monocytes in the bovine peripheral blood.

The invention discloses a method for extracting albumin and globulin from bovine serum and a method for utilizing ox blood and relates to the technical field of protein extraction. According to the method for extracting albumin and globulin from bovine serum provided by the invention, the principle of plasma protein depositing in saline solution in a certain saturability is utilized, (NH4)2SO4 fractional precipitation is adopted and the bovine serum albumin is further purified. The bovine serum albumin is high in purity, is low in endotoxin content and meets the requirement of Chinese Biological Product Regulation. A saturated (NH4)2SO4 fractional repeated salting out method is utilized to extract the bovine serum albumin; the high molecular weight plasma protein is extremely removed; and the purity of the extracted bovine serum albumin is high. The method for extracting albumin and globulin from bovine serum provided by the invention is simple in process; no special device is required; the method is suitable for expanded production; and the method for utilizing ox blood provided by the invention can increase the use ratio of the ox blood.

The invention discloses a separation method of bovine blood CD4 + T lymphocytes, and relates to the field of bioengineering.The separation method comprises the following steps that oxtail vein blood is collected; separating lymphocytes by using a commercial lymphocyte separation kit; centrifuging, collecting precipitate, and adding a red blood cell lysis solution to lyse red blood cells; adding a culture medium, washing, centrifuging and collecting precipitate; after cell counting, adding a primary antibody of CD4 + T lymphocytes for incubation; adding a magnetic bead secondary antibody for incubation; and finally, separating the CD4 + T lymphocytes by using a magnetic column. The invention introduces the bovine CD4 + T lymphocyte separation method for the first time, and fills the blank of the test method technology in the field. The bovine CD4 < + > T lymphocytes obtained by the method are good in activity, large in quantity and high in purity. The kit can be widely applied to clinical diagnosis besides scientific research, and has a good market prospect.

A pretreatment method for measuring lead in blood by graphite furnace atomic absorption process belongs to the field of a detection method of lead content in blood and especially relates to a pretreatment method for measuring content of lead in blood by graphite furnace atomic absorption process. The method comprises preparation of a matrix modifier and preparation of a lead standard solution. The matrix modifier contains ammonium biphosphate, Triton and nitric acid. The method comprises the following steps: Step 1, dissolving a freeze-dried bovine blood lead standard substance as required; Step 2, successively adding nitric acid, Triton and ammonium biphosphate, and stabilizing for 1-2 days; Step 3, successively adding whole blood, nitric acid and hydrogen peroxide into the matrix modifier so as to prepare a measuring sample; and Step 4, treating the measuring sample by water bath for 1-2 hours until the sample turns faint yellow from atropurpureus for measurement. The pretreatment method provides a method for accurately measuring content of lead in blood. The pretreatment method is simple to operate, has good stability and high precision, and eliminates interference of blood sample matrix composition.

The invention discloses fertilizer for production of high-Fe peaches. The fertilizer comprises 40-45 parts of kiwi fruit tree leaves, 30-35 parts of peach tree leaves, 20-25 parts of chicken blood, 20-25 parts of porcine blood, 15-20 parts of bovine blood, 30-35 parts of dried fowl manure, 15-20 parts of soybean cakes, 10-15 parts of plant ash, 5-7 parts of ferrous sulfate, 3-5 parts of an EM stock solution, 0.5-0.8 parts of molasses and 2-4 parts of a deodorant. The high-Fe peaches have the functions of invigorating qi and blood, nourishing yin and generating body fluid and can be applied to people with deficiency of Qi and blood, emaciation with sallow complexion, palpitation and short breath after serious illnesses; the high-Fe peaches have high Fe content and are ideal auxiliary food for patients with iron-deficiency anemia.

The invention discloses a smoked sausage. The smoke sausage comprises the following raw materials in parts by weight: 40 to 60 parts of duck breast meat, 25 to 28 parts of beef, 1 to 3 parts of garlic, 1 to 3 parts of ginger, 1 to 3 parts of edible salt, 1 to 3 parts of liquorice, 0.2 to 0.8 part of sodium hexametaphosphate and sodium tripolyphosphate, 0.5 to 1.5 parts of sodium citrate, 0.3 to 0.5 part of ground cinnamon, 4 to 6 parts of degreased milk powder, 0.2 to 0.5 part of allspice powder, 0.1 to 0.2 part of a sausage forming agent and 10 to 18 parts of salting liquor. The invention also discloses a processing process of the smoked sausage. The processing process comprises the steps of weighing raw materials, salting, mincing meat, chopping, filling a casing, smoking, steaming and packaging. Compared with a traditional process, for the processing process disclosed by the invention, the meat is preserved and salted through the salting liquor, and a finished product obtained by adopting a special cattle blood glutinous rice process is fresh and delicious in taste, reddish in appearance, moderate in brightness and not greasy.

The invention discloses a primer composition for detecting bovine citrullinemia harmful genes and a kit and an application thereof. The primer composition disclosed by the invention is composed of a primer set A and a primer set B, wherein the primer set A is composed of a primer 1 and a primer 2, and the primer set B is composed of a primer 3 and a primer 4; and the nucleotide sequences of the primer 1 to the primer 4 are respectively shown in SEQ ID NO.1-4. The invention also provides a kit containing the primer combination, a method for detecting bovine citrullinemia harmful genes by using the kit provided by the invention comprises the following steps: extracting the whole set of genomic DNA in bovine blood to serve as a template, carrying out nested PCR (Polymerase Chain Reaction) and sequencing the obtained PCR products. The base change of a mutation site can be directly controlled according to the sequencing result, thereby ensuring the result accuracy and meeting the requirements of detection technology with such characteristics as high speed, accuracy, high throughput and the like.

The invention discloses a traditional Chinese medicinal composition for treating chocolate cyst. The traditional Chinese medicinal composition is prepared from the following medicines by weight: 13g of Chinese angelica, 12g of cistanche deserticola, 6g of waxgourd seed, 11g of platycladi seed, 20g of cornu bubali, 10g of willow root, 15g of pork intestines, 20g of fresh bovine blood, 1g of dark plum, 200g of washing water of rice, 15g of ginger, 10g of diaphragma juglandis, 20g of lard oil, 30g of wheat bran. A preparation method comprises the following steps: wrapping 6g of waxgourd seed, 11g of platycladi seed, 20g of cornu bubali and 10g of willow root with the pork intestines, adding 200g of washing water of rice for decocting, keeping boiling for 20 minutes and filtering liquid medicine to obtain liquid medicine A; sequentially adding 13g of Chinese angelica and 10g of diaphragma juglandis into the liquid medicine A, decocting to boiling, and keeping boiling for 20 minutes; finally, simultaneously adding 12g of cistanche deserticola and 1g of dark plum, and filtering liquid medicine to obtain an oral medicine.

The invention relates to a composition for improving anemia as well as functional food, a preparation method and application thereof. The composition for improving anemia comprises the following raw materials in parts by weight: 5-25 parts of bovine bone marrow polypeptide powder, 10-30 parts of red date powder, 20-40 parts of bovine blood protein polypeptide powder, 12-25 parts of Chinese wolfberry fruit powder and 13-27 parts of fructo-oligosaccharide. According to the composition for improving anemia, starting from the reasons of absorption and utilization rate of iron in iron-deficiency anemia, recovery of hematopoietic function and the like, the aim of effectively adjusting and improving anemia conditions of anemia crowds can be achieved through mutual cooperation and synergistic effects of multiple components. The composition is high in effective rate and good in safety for anemia caused by iron deficiency in diet, excessive dieting or irregular diet, digestive tract function weakening, poor nutrient absorption, aplastic anemia and the like.

The invention belongs to the technical field of industrial labor protection, and particularly relates to an anti-splashing, easy-to-clean isolation and collection device in a beef cattle slaughteringworkshop. In the prior art, beef cattle need to be drained after being slaughtered, and devices are easy to cause splashing, and cannot meet the quantity requirement of blood draining. The device of the invention is provided with two blood draining tanks which are respectively used for receiving bovine blood and soiling liquid; an A-shaped isolation part is arranged above the side plate which is arranged between the two blood draining tanks; two sides of the isolation part are provided with anti-splashing parts; the isolation part and the anti-splashing parts are arranged in parallel along theblood draining tanks; the isolation part and the two anti-splashing parts respectively form a bovine blood channel and a soiling liquid channel; and the isolation part is provided with an operating rod which is assembled on an adjusting guide rail through a sliding block, so that the operating rod drives the isolation part to be adjusted left and right and/or up and down. According to the invention, the isolation part and the anti-splashing parts are adopted to form the two channels of the bovine blood channel and the soiling liquid channel so as to realize separate collection and treatment on the bovine blood and the soiling liquid; and the isolation part is provided with the adjusting guide rail which is capable of being adjusted horizontally and vertically and can adjust the hanging height of cattle.

The invention discloses a targeted anti-bladder-cancer albumin carrying mitomycin drug and a preparation method thereof. The targeted anti-bladder-cancer albumin carrying mitomycin drug is characterized in that drug molecules contain albumin molecules, anti-human-bladder-cancer monoclonal antibodies (BDI-1) and mitomycin (MMC), and the synthetic method of the drug molecules comprises the following steps: using glutaraldehyde as a cross-linking curing agent, and combining mitomycin molecules with bovine blood albumin molecules to be prepared into albumin carrying mitomycin (MMC-NS) nanoparticles with the particle size being 280-350 nanometer; then using a heterogenic bifunctional coupling agent N-succinimido-3-(2-pyridyl disulfide) propionate (SPDP) to causes the albumin carrying mitomycin molecules to be coupled with a pyridyl disulfide group (PDP) to obtain MMC-NS-PDP molecules; and finally, causing MMC-NS-PDP to reacting with BDI-1-SH molecules to obtain the drug with the molecules containing anti-human-bladder-cancer antibodies, the albumin molecules and the mitomycin, namely the targeted anti-bladder-cancer albumin carrying mitomycin drug, (BDI-1-MMC-NS for short). The drug has strong targeting to bladder cancer, a strong curative effect on bladder cancer, and a smaller influence on normal tissue cells.

The invention relates to a freeze-drying protective additive formula of a feline panleukopenia attenuated freeze-dried vaccine. The freeze-drying protective additive contains the following components and contents: 0.3 percent of ox blood albumin, 3 percent of mannitol, 2 percent of cane sugar, 1 percent of dextran, 0.13 percent of dipostassium phosphate, 0.045 percent of monopotassium phosphate, 0.5 percent of vitamin C, 0.0625 percent of amphotericin and the balance of water. The freeze-drying protective additive has the advantages that the property, water content, solubleness and titer of the feline panleukopenia attenuated freeze-dried vaccine are consistent with the requirements of Veterinary Pharmacopoeia of the People Republic of China III by selecting the appropriate freeze-drying protective additive, and the quality guarantee peroid of the feline panleukopenia attenuated freeze-dried vaccine is 2 years.