Application of earthworm fibrinolytic enzyme in resistance to liver fibrosis

An anti-hepatic fibrosis and plasminase technology, applied in the biological field, can solve the problems of accelerating the degradation of liver collagen, achieve high biological activity, relieve symptoms, and have no toxic and side effects

Inactive Publication Date: 2010-01-20
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But can it hydrolyze the extracellular matrix (ECM) of the fibrotic liver or / and activate the zymogens of MMPs, thereby accelerating the degradation of liver collagen? There are no reports of this yet

Method used

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  • Application of earthworm fibrinolytic enzyme in resistance to liver fibrosis
  • Application of earthworm fibrinolytic enzyme in resistance to liver fibrosis
  • Application of earthworm fibrinolytic enzyme in resistance to liver fibrosis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0045] 1. Determination of the activity of "Dilong 2" and its plasmin and collagenase components

[0046] Weigh "Dilong No. 2" and dissolve it in 0.01mol / L pH 7.2 Tris-HCl to form a 10% solution. Equilibrate the Sephadex G75 gel chromatography column with the same Tris-HCl buffer, load samples in batches, and elute with the same buffer , collect and combine the elution I peak and the elution II peak respectively, measure the volume of the eluent respectively, and measure as follows:

[0047] ① Prepare fibrin-agarose plates with reference to the assay method of lumbrokinase bioactivity (Hao Suli, Shen Jia et al., Research on the assay method of lumbrokinase bioactivity, China Pharmaceutical Affairs [J]. 1996, 10(6): 403-405). Place the plate at room temperature for 1 hour, punch holes, spot 10 μl per well, and incubate at 37°C for 8 hours. Take out the plate carefully, stain with Coomassie brilliant blue, decolorize with 7% glacial acetic acid, and the degraded parts of fibrin ...

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Abstract

The invention relates to an application of an earthworm fibrinolytic enzyme in preparation of liver fibrosis resistant medicine or liver protection health-care food, which belongs to the technical field of biology. The effective dose of an oral liver fibrosis resistant earthworm fibrinolytic enzyme is 1,000-3,000 urokinase unit / person / day. Proved by experiments, earthworm collagenase can degrade collagen but cannot degrade (ox blood) fibrin, and the earthworm fibrinolytic enzyme can degrade the (ox blood) fibrin and can also degrade the collagen, thus the activity of the earthworm fibrinolytic enzyme is higher than the activity of the earthworm collagenase and the activity of a collagenase standard product (Sigma I type). Proved by a liver fibrosis resistant experiment by using a rat, the oral liver fibrosis resistant earthworm fibrinolytic enzyme has obvious effect and no obvious poisonous and side effect, but the oral liver fibrosis resistant earthworm collagenase does not have obvious effect.

Description

1. Technical field [0001] The invention relates to the application of earthworm fibrinolytic enzyme (ie lumbrokinase) in resisting liver fibrosis, and belongs to the field of biotechnology. 2. Background technology [0002] 1. Liver fibrosis is the common pathological basis of all chronic liver diseases, and it is the central link that must pass through in the development of liver cirrhosis. Liver fibrosis is a pathological process in which the extracellular matrix (mainly collagen, followed by structural glycoproteins, glycosaminoglycans, and elastin) of hepatic cells is more secreted than degraded, resulting in excessive deposition of extracellular matrix. [0003] The etiology of liver fibrosis can be roughly divided into infectious (chronic B, C and D viral hepatitis, schistosomiasis, etc.), chemical metabolic defects (chronic alcoholic liver disease, chronic drug-induced liver disease), congenital metabolic defects and autoimmune hepatitis etc. [0004] As far as hepa...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/48A61K35/64A61P1/16A23L1/305A23L33/10A61K35/62
Inventor 张治国张晓蓉连桂芳
Owner NANJING AGRICULTURAL UNIVERSITY
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