6 results about "Lumbrokinase" patented technology

The invention discloses a plasmin lumbrukinase peptide dietary therapy formula suitable for rehabilitation of cardiovascular and cerebrovascular diseases, blood circulation promoting, blood stasis removing, thrombolysis, collateral dredging and microcirculation improving, and relates to the technical field of medicine and food homology dietary therapy. Soybean protein isolate and earthworm protein are used as core thrombolytic active raw materials, isomaltooligosacharide, xylitol, German Jilida collagen peptide, soybean peptide powder, Belgian imported inulin, red yeast rice and natto powder are compounded, microcrystalline cellulose and magnesium stearate are used as auxiliary materials, and probiotics / metabiotics strains such as rhamnolactobacilli JYLR-005 and the like can be selectively added. The raw materials have a synergistic effect through precise matching, plasmin and lumbrukinase peptide in earthworm protein can directly dissolve thrombus fibrin, natto powder and red yeast rice assist in lowering lipid and dissolving thrombus, multiple active peptides and prebiotics regulate intestinal microecology to improve microcirculation of the whole body, probiotics / metabiotics further strengthen the metabolic capacity of the body, no chemical medicine is added in the whole process, and the traditional Chinese medicine composition is free of toxic and side effects and free of toxic and side effects. The safety is high; the dietary therapy composition can achieve postoperative rehabilitation conditioning of cardiovascular and cerebrovascular diseases through daily consumption, effectively promote blood circulation to remove blood stasis, dissolve thrombus and dredge collaterals, improve microcirculation and relieve various discomfort caused by blood vessel clogging, raw materials are easy to obtain, compatibility is scientific, and the dietary therapy composition is suitable for large-scale production and popularization.

The invention provides a pharmaceutical composition for improving bone mineral density and a preparation method thereof, and belongs to the technical field of biological medicine, the pharmaceutical composition comprises lactarius vomica extract peptide, winged yam rhizome peel extract, rainbow trout extract, calcium citrate-glycine chelate, vitamin K2 and vitamin D3. The lactarius volemus extract peptide is obtained by sequentially carrying out enzymolysis on lactarius volemus by bromelain and subtilisin and purifying and extracting enzymatic hydrolysate through an LH-60 sephadex column. The winged yam rhizome peel extract is obtained by extracting the peels of winged yam rhizome tubers with an ethanol water solution and purifying and extracting the extracting solution with an AB-8 macroporous adsorption resin column. The rainbow trout extract is a product below 5kDa obtained by sequentially performing enzymolysis on fish heads, fish skins and fish scales of rainbow trout through trypsin and lumbrukinase. All the components are compounded according to a specific proportion, and the core effect of improving the bone mineral density is achieved through two-way regulation and control of bone metabolism, optimization of calcium utilization and improvement of safety.

The invention belongs to the technical field of biological pharmacy, and particularly relates to a purification method of lumbrukinase suitable for an injection. Affinity chromatography and ion exchange chromatography are adopted, and impurities such as viruses and 45k brown lumbrukinase are removed; the lumbrukinase obtained by purification is identified as proteolytic enzyme, has dual pharmacological effects of thrombolysis and fibrinogen reduction, is free of adverse reactions such as viruses, heat sources and allergy, can be prepared into powder injection and water injection, and can be used as a medicine for treating thrombolysis and fibrinogen reduction. The pharmaceutical composition can be used for thrombolysis treatment of acute myocardial infarction and acute cerebral infarction in a large dose of 1,000,000-2,400 million units, and can also be used for defibrination treatment of acute cerebral infarction in a small dose of 25,000 units. In addition, raw materials are rich in source, low in price and suitable for industrial production.

The invention relates to the technical field of enzyme engineering and biological fermentation, in particular to an extraction method of traditional Chinese medicine lalang grass rhizome. By combining the composite enzymatic hydrolysate with ultrasonic-assisted microbial fermentation, the lalang grass rhizome polysaccharide is extracted, and the problems that a traditional method is low in efficiency, insufficient in purity and damaged in activity are solved. The composite enzymatic hydrolysate is prepared by mixing enzyme digestion modified lumbrukinase (prepared by immobilized trypsin digestion modification) and neutral pectinase according to a ratio of 1: 1, and the microbial fermentation adopts a bacillus licheniformis and saccharomyces cerevisiae composite strain according to a ratio of 5: 1. Compared with a traditional method, the method has the advantages that the polysaccharide extraction rate reaches 67.36%-67.5% and is increased by about 6.5%-7%, the precision RSD is smaller than 1.5%, the product is stable within 12 h, no strong acid or strong alkali or toxic organic solvent exists, the safety standard is met, the polysaccharide activity is reserved, and the method has the industrialization prospect.

The application belongs to the technical field of biological medicine, and discloses a thrombolytic composition containing plant extracts and biological enzymes, and a preparation method and application thereof. The composition contains the following components in parts by weight: natto kinase 50-200 parts; lumbrukinase 10-50 parts; angelica extract 10-100 parts; turmeric extract 10-100 parts; brown algae extract 5-50 parts; protective agent: mannitol 0.2-0.5 parts, edible gelatin 0.5-5 parts, and sodium bicarbonate 0.1-2 parts. The application realizes the synergistic antithrombotic effect of multiple mechanisms and multiple targets through the direct thrombolytic effect of natto kinase and lumbrukinase, and the anti-platelet aggregation, anti-inflammatory and anticoagulation effects of angelica, turmeric and brown algae, and improves the oral bioavailability. The ingredients with the same origin of medicine in the formula help to improve the microcirculation and protect the vascular endothelium. Compared with traditional chemical drugs, the composition has higher safety and smaller side effects, and is suitable for long-term prevention and rehabilitation.

The invention discloses a high-acid-resistance lumbrukinase enteric-coated pellet and a preparation method thereof, and relates to the technical field of pharmacy. The preparation method comprises the following steps: S1, mixing lumbrukinase dry powder with blank mixed powder to obtain lumbrukinase mixed powder; s2, blank pellets with the particle size of 900-1400 microns, an adhesive and lumbrukinase mixed powder are mixed, medicine wrapping and drying are conducted, and medicine-containing pellets are obtained; s3, the drug-containing pellets are subjected to enteric coating, the moisture content of the coated pellets is smaller than or equal to 8%, and the drug-containing pellets are obtained. By controlling the particle size and the moisture after coating, the acid resistance of the prepared lumbrukinase is larger than 97% and is still higher than 90% after being accelerated for 6 months, the stability is remarkably improved, and the titer of the lumbrukinase is more stable.