21 results about "Lumbrokinase" patented technology

The invention relates to an oral mucosa repairing medicine and a preparation method thereof, and belongs to the technical field of medicines, the medicine comprises barnacle peptide, salivary lactobacillus, resveratrol, a biphasic adhesion matrix, hydroxyapatite, vitamin B family and other components. The preparation method comprises the following steps: sequentially carrying out enzymolysis on barnacle gooseneck by pepsin and lumbrukinase to obtain barnacle peptide. The double-phase adhesion matrix comprises a water-phase matrix, an oil-phase matrix and lecithin; the water-phase matrix contains a chondroitin sulfate-chitosan quaternary ammonium salt compound, sodium alginate and other components; the oil-phase matrix comprises sesame oil, beeswax, a polylactic acid-glycolic acid copolymer and the like. According to the invention, barnacle peptide and salivary lactobacillus are prepared by adopting a special method and are matched with resveratrol for use, so that multi-target and multi-path promotion of oral mucosa repair can be realized; the chondroitin sulfate-chitosan quaternary ammonium salt compound is prepared through electrostatic and physical embedding, the chondroitin sulfate-chitosan quaternary ammonium salt compound is matched with other components to prepare the biphasic adhesion matrix, the biocompatibility is good, the adhesion is high, and the action time of a sustained-release drug is well prolonged.

The invention discloses a preparation method and application of yak blood peptide with anti-hypoxia and anti-fatigue functions. The method comprises the steps of raw material pretreatment, composite enzymolysis (adopting a mixture of enzyme digestion modified lumbrukinase and nattokinase), enzyme deactivation, separation and purification, ferrous chelation, Maillard reaction, concentration and drying and the like. The enzymolysis efficiency and the active peptide yield are remarkably improved through ultrasonic treatment and a composite enzymolysis technology, the stability and the anti-hypoxia capacity of peptide fragments are enhanced through ferrous chelation, and the antioxidant activity and the anti-fatigue activity are further improved through the Maillard reaction. Experiments show that the yak blood peptide prepared by the method can significantly improve the antioxidant enzyme activity, prolong the normal-pressure hypoxia survival time, prolong the swimming exhaustion time and significantly reduce the MDA content of brain tissue and myocardial tissue. The obtained product can be applied to anti-hypoxia and anti-fatigue medicines or health-care foods, and has the advantages of high process efficiency, remarkable activity, high safety and the like.

The invention relates to a skin wound repairing material and a preparation method thereof, and belongs to the technical field of biological medicines. The repair material disclosed by the invention contains palmitoyl tripeptide-1, methacrylated collagen, cuttlebone extract, recombinant human epidermal growth factor lipidosome, polydopamine nanoparticles, hyaluronic acid, sodium alginate modified silk fibroin and the like in a specific proportion. Wherein the cuttlebone extract is a product which is obtained by carrying out common enzymolysis on cuttlebone through chitinase and nattokinase and then carrying out step-by-step enzymolysis on the cuttlebone through gelatinase and lumbrukinase and is less than 5kDa. The components are prepared into an efficient product by adopting a special method, the stability of the components is improved, the problem that the activity is difficult to maintain is solved, and the skin wound repairing effect is good through reasonable compatibility.

The invention discloses a plasmin lumbrukinase peptide dietary therapy formula suitable for rehabilitation of cardiovascular and cerebrovascular diseases, blood circulation promoting, blood stasis removing, thrombolysis, collateral dredging and microcirculation improving, and relates to the technical field of medicine and food homology dietary therapy. Soybean protein isolate and earthworm protein are used as core thrombolytic active raw materials, isomaltooligosacharide, xylitol, German Jilida collagen peptide, soybean peptide powder, Belgian imported inulin, red yeast rice and natto powder are compounded, microcrystalline cellulose and magnesium stearate are used as auxiliary materials, and probiotics / metabiotics strains such as rhamnolactobacilli JYLR-005 and the like can be selectively added. The raw materials have a synergistic effect through precise matching, plasmin and lumbrukinase peptide in earthworm protein can directly dissolve thrombus fibrin, natto powder and red yeast rice assist in lowering lipid and dissolving thrombus, multiple active peptides and prebiotics regulate intestinal microecology to improve microcirculation of the whole body, probiotics / metabiotics further strengthen the metabolic capacity of the body, no chemical medicine is added in the whole process, and the traditional Chinese medicine composition is free of toxic and side effects and free of toxic and side effects. The safety is high; the dietary therapy composition can achieve postoperative rehabilitation conditioning of cardiovascular and cerebrovascular diseases through daily consumption, effectively promote blood circulation to remove blood stasis, dissolve thrombus and dredge collaterals, improve microcirculation and relieve various discomfort caused by blood vessel clogging, raw materials are easy to obtain, compatibility is scientific, and the dietary therapy composition is suitable for large-scale production and popularization.

The invention provides an anti-aging and skin-tightening composition and a preparation method thereof, and belongs to the technical field of anti-aging and skincare, and the composition comprises a hovenia acerba infructescence extract, an okra seed extract, tetrapeptide-4, acetyl octapeptide-1, a hill-pit snail extract, paeonol and a carnosine-zinc gluconate chelate. And carrying out aerobic fermentation on the raisin tree infructescence powder through aspergillus oryzae, and purifying the centrifuged supernatant through a composite filler resin column to obtain the raisin tree infructescence extract. The preparation method comprises the following steps: sequentially carrying out enzymolysis on okra seeds by virtue of compound enzyme, ficin and subtilisin, adding absolute ethyl alcohol for extraction, centrifuging supernate, and purifying by virtue of an LH-20 sephadex column, so as to obtain the okra seed extract. The preparation method comprises the following steps: sequentially carrying out enzymolysis on the mountain snail meat through trypsin and lumbrukinase, centrifuging the enzymatic hydrolysate, and carrying out ultrafiltration on the supernate to obtain the mountain snail extract with the molecular weight of less than 3kDa. The components are matched for use, so that the balance of high-efficiency anti-aging and low-irritation can be realized through synergism of three major pathways of inhibiting collagen degradation, promoting collagen synthesis and resisting sugar and relieving.

The utility model provides a connecting part for lumbrukinase freeze-drying monitoring, which comprises a vacuumizing pipeline used for connecting a drying box and a monitoring device, the vacuumizing pipeline comprises a first pipeline with one end used for connecting the drying box and a second pipeline with one end connected with the first pipeline and the other end connected with the monitoring device, a filter part is arranged between the first pipeline and the second pipeline and comprises a steel ring and a filter screen arranged on the steel ring, the outer side of the filter screen is fixedly connected with the inner ring side wall of the steel ring, one side of the steel ring extends outwards to form a limiting ring edge, the inner wall of the end, close to the first pipeline, of the second pipeline is sunken inwards to form a groove, and the groove is matched with the outer diameter of the steel ring and used for containing the steel ring. An annular groove matched with the limiting ring edge is formed in the bottom of the groove. According to the connecting part for the freeze-drying monitoring of the lumbrukinase, the problem that a connecting part for the freeze-drying monitoring of the lumbrukinase, which is high in sealing performance and capable of preventing impurities from entering a freeze-drying mass spectrometer, is lacked in the prior art is solved.

The invention discloses high-activity lumbrukinase extraction equipment and method. The equipment comprises an equipment bracket, a cleaning assembly, a grinding assembly and a separation assembly, the cleaning assembly comprises a first transverse plate, a cleaning outer cylinder arranged on the first transverse plate and an inner net cylinder arranged in the cleaning outer cylinder in a sleeved mode. A gland is arranged at the top end of the outer cleaning cylinder and internally provided with a cleaning coil pipe. The grinding assembly comprises a second transverse plate, a grinding seat arranged on the second transverse plate, a rotating seat clamped in the grinding seat and a grinding motor for providing power for the grinding seat; the separation assembly comprises a separation barrel, a conical separation mesh enclosure and a material stirring fan blade which are arranged in the separation barrel, and a material stirring motor for providing power for the material stirring fan blade; the earthworm adults are cleaned, ground and separated, so that the lumbrukinase in the earthworm bodies is completely separated, and the purity and activity of the lumbrukinase are effectively improved; meanwhile, due to the integrated equipment structure, the extraction efficiency of the lumbrukinase is greatly improved.

The invention provides a pharmaceutical composition for improving bone mineral density and a preparation method thereof, and belongs to the technical field of biological medicine, the pharmaceutical composition comprises lactarius vomica extract peptide, winged yam rhizome peel extract, rainbow trout extract, calcium citrate-glycine chelate, vitamin K2 and vitamin D3. The lactarius volemus extract peptide is obtained by sequentially carrying out enzymolysis on lactarius volemus by bromelain and subtilisin and purifying and extracting enzymatic hydrolysate through an LH-60 sephadex column. The winged yam rhizome peel extract is obtained by extracting the peels of winged yam rhizome tubers with an ethanol water solution and purifying and extracting the extracting solution with an AB-8 macroporous adsorption resin column. The rainbow trout extract is a product below 5kDa obtained by sequentially performing enzymolysis on fish heads, fish skins and fish scales of rainbow trout through trypsin and lumbrukinase. All the components are compounded according to a specific proportion, and the core effect of improving the bone mineral density is achieved through two-way regulation and control of bone metabolism, optimization of calcium utilization and improvement of safety.

The present invention relates to a composition for bone repair and growth and a preparation method thereof, belonging to the field of medical technology. The composition includes a peptide extracted from the gelatinous layer of sand jellyfish and a bamboo yellow-Grifola frondosa composite bacterial peptide; the peptide extracted from the gelatinous layer of sand jellyfish contains a product between 500Da and 2000Da obtained by sequentially hydrolyzing the gelatinous layer of sand jellyfish with lumbrokinase, serrapeptase, and transglutaminase; the bamboo yellow-Grifola frondosa composite bacterial peptide contains a product between 500Da and 2000Da obtained by co-enzymatic hydrolysis of bamboo yellow mushrooms and Grifola frondosa mushrooms with cellulase and chitinase, then co-enzymatic hydrolysis with nattokinase and serrapeptase, and finally co-enzymatic hydrolysis with aminopeptidase and transglutaminase. The present invention adopts a special method to prepare the peptide extracted from the gelatinous layer of sand jellyfish and the bamboo yellow-Grifola frondosa composite bacterial peptide, and the combined use achieves good biological activity, stability, and absorption utilization rate, overcomes the defects of existing bone repair and growth drugs, and effectively promotes bone repair and growth.

The present invention relates to a pharmaceutical composition for reducing uric acid and a preparation method thereof, belonging to the field of biotechnology. The pharmaceutical composition contains earthworm peptides, fungal fermentation products, fenugreek seed extract, chicory extract, and plantain extract; the earthworm peptides contain products obtained by successively subjecting earthworms to hierarchical enzymatic hydrolysis with trypsin, lumbrokinase, and nattokinase; the fungal fermentation products contain products obtained by jointly fermenting the pulp of cornel and smilax glabra rhizome with bacillus subtilis and bifidobacterium adolescentis; the fenugreek seed extract contains products obtained by successively subjecting fenugreek seed kernels to hierarchical enzymatic hydrolysis with ficin, trypsin, and papain; the chicory extract is an ethanol percolation extract of chicory roots; the plantain extract is an ethanol percolation extract of plantain. The combined use of each component has good uric acid reducing and anti-inflammatory effects, can quickly and efficiently relieve inflammation and reduce the blood uric acid content, and is safe and reliable.

The embodiment of the invention discloses an automatic mixing, filling and detecting method and device for medicine capsules containing earthworms and astragalus membranaceus, and the method comprises the steps that when the medicine capsules containing the earthworms and the astragalus membranaceus are filled, medicine powder for mixing the earthworms and the astragalus membranaceus is filled into all the medicine capsules; detecting the component content of the lumbrukinase in each medicine capsule, and screening out a first medicine capsule meeting the requirement of the lumbrukinase content; detecting the content of the astragaloside in the first medicine capsule, and screening out a second medicine capsule meeting the content requirement of the astragaloside; performing granularity analysis on the medicine powder in the second medicine capsule to obtain a granularity analysis result; and based on an analysis result of the granularity, screening out a third medicine capsule of which the granularity accords with a preset threshold value from the second medicine capsules so as to complete automatic mixing and filling detection of the medicine capsules.

The invention belongs to the technical field of biological pharmacy, and particularly relates to a purification method of lumbrukinase suitable for an injection. Affinity chromatography and ion exchange chromatography are adopted, and impurities such as viruses and 45k brown lumbrukinase are removed; the lumbrukinase obtained by purification is identified as proteolytic enzyme, has dual pharmacological effects of thrombolysis and fibrinogen reduction, is free of adverse reactions such as viruses, heat sources and allergy, can be prepared into powder injection and water injection, and can be used as a medicine for treating thrombolysis and fibrinogen reduction. The pharmaceutical composition can be used for thrombolysis treatment of acute myocardial infarction and acute cerebral infarction in a large dose of 1,000,000-2,400 million units, and can also be used for defibrination treatment of acute cerebral infarction in a small dose of 25,000 units. In addition, raw materials are rich in source, low in price and suitable for industrial production.

The invention relates to an enzyme preparation composition for improving the soil fertility of saline-alkali soil. The enzyme preparation composition is prepared from the following components in parts by mass: 45 to 55 parts of lumbrukinase, 1.8 to 2.2 parts of earthworm active protein, 1.8 to 2.2 parts of perphosphatase, 4.5 to 5.5 parts of polyglutamic acid, 22.5 to 27.5 parts of humic acid, 1.8 to 2.2 parts of bacillus subtilis, 4.5 to 5.5 parts of silicon element, 4.5 to 5.5 parts of calcium element, 2.7 to 3.3 parts of zinc element and 0.9 to 1.1 parts of magnesium element. The enzyme preparation composition disclosed by the invention has the advantages that the enzyme preparation composition is prepared from the comprehensive aspects of supplementing high-quality organic matters and nutritional ingredient varieties in soil, reducing the toxicity of sodium ions, stimulating the stress resistance of crops, inhibiting soil-borne fungi, stabilizing the salinity and alkalinity of the soil, lowering the pH value, improving the granular structure and permeability of the soil, prolonging the retention and release time of nutrients and the like; various physicochemical properties of the saline-alkali soil are synergistically improved, so that the output value of saline-alkali soil resources is improved.

The invention relates to the technical field of enzyme engineering and biological fermentation, in particular to an extraction method of traditional Chinese medicine lalang grass rhizome. By combining the composite enzymatic hydrolysate with ultrasonic-assisted microbial fermentation, the lalang grass rhizome polysaccharide is extracted, and the problems that a traditional method is low in efficiency, insufficient in purity and damaged in activity are solved. The composite enzymatic hydrolysate is prepared by mixing enzyme digestion modified lumbrukinase (prepared by immobilized trypsin digestion modification) and neutral pectinase according to a ratio of 1: 1, and the microbial fermentation adopts a bacillus licheniformis and saccharomyces cerevisiae composite strain according to a ratio of 5: 1. Compared with a traditional method, the method has the advantages that the polysaccharide extraction rate reaches 67.36%-67.5% and is increased by about 6.5%-7%, the precision RSD is smaller than 1.5%, the product is stable within 12 h, no strong acid or strong alkali or toxic organic solvent exists, the safety standard is met, the polysaccharide activity is reserved, and the method has the industrialization prospect.

The invention belongs to the technical field of biology, and particularly provides a method for heterologous expression of lumbrukinase based on Escherichia coli, which comprises the following steps: firstly connecting a lumbrukinase gene with a vector to construct a recombinant expression vector, then transforming the recombinant expression vector into a competent strain to obtain a recombinant bacterium, then culturing the recombinant bacterium, and inducing expression protein, thereby obtaining the lumbrukinase heterologous expression vector based on Escherichia coli. Purifying to obtain lumbrukinase protein; data prove that the lumbrukinase protein obtained through expression of the method is high in activity and high in thrombolysis capacity, the lumbrukinase obtaining time can be greatly shortened, and the lumbrukinase yield can be greatly increased.

The invention provides a traditional Chinese medicine composition for treating pulmonary fibrosis and a preparation method of the traditional Chinese medicine composition. The preparation method comprises the following steps: accurately weighing and pretreating medicinal materials, decocting and extracting oysters, decocting and extracting water-soluble components with water, extracting fat-soluble components of earthworms with alcohol, mixing and refining, and forming a preparation. A differential extraction process is adopted, the dissolution rate of effective components of mineral medicines and animal medicines is increased, for example, the dissolution rate of calcium salt is increased by 35% by decocting oysters firstly, and the extraction efficiency of liposoluble components of lumbrukinase is remarkably enhanced by alcohol extraction of earthworms. Tannin impurities are removed through chitosan flocculation, and component stability is guaranteed through vacuum concentration. And finally, various dosage forms such as granules, decoction or tablets can be prepared, different clinical requirements are met, and the preparation method has the advantages of scientific process, controllable quality and convenience in application.

Particle structure of an active ingredient from redworms for improving blood circulation, wherein the particle structure of an active ingredient from redworms (1) comprises: an active nuclear body (11), the main component of which is lumbrokinase obtained from red worms; and a protective surface layer (12) surrounding the active core body (11) to prevent the external environment from influencing the activity of the active core body (11), and thus to improve the storage stability of the particle structure of an active ingredient from red worms (1) and its absorption rate by the human body.

The present invention relates to a skin wound repair material and a preparation method thereof, belonging to the field of biomedicine. The repair material of the present invention contains, among other ingredients, palmitoyl tripeptide-1, methacryloylated collagen, cuttlebone extract, recombinant human epidermal growth factor liposomes, polydopamine nanoparticles, hyaluronic acid, and sodium alginate-modified silk fibroin in specific proportions. The cuttlebone extract comprises a product of less than 5 kDa obtained by co-enzymatic hydrolysis of cuttlebone with chitinase and nattokinase, followed by stepwise enzymatic hydrolysis with chymotrypsin and lumbrokinase. Each component is prepared using a special method to obtain a highly effective product, which improves the stability of the ingredients and solves the problem of difficulty in maintaining their activity. Through rational combination, the product has a good skin wound repair effect.

The application belongs to the technical field of biological medicine, and discloses a thrombolytic composition containing plant extracts and biological enzymes, and a preparation method and application thereof. The composition contains the following components in parts by weight: natto kinase 50-200 parts; lumbrukinase 10-50 parts; angelica extract 10-100 parts; turmeric extract 10-100 parts; brown algae extract 5-50 parts; protective agent: mannitol 0.2-0.5 parts, edible gelatin 0.5-5 parts, and sodium bicarbonate 0.1-2 parts. The application realizes the synergistic antithrombotic effect of multiple mechanisms and multiple targets through the direct thrombolytic effect of natto kinase and lumbrukinase, and the anti-platelet aggregation, anti-inflammatory and anticoagulation effects of angelica, turmeric and brown algae, and improves the oral bioavailability. The ingredients with the same origin of medicine in the formula help to improve the microcirculation and protect the vascular endothelium. Compared with traditional chemical drugs, the composition has higher safety and smaller side effects, and is suitable for long-term prevention and rehabilitation.

The present invention relates to a repair drug for oral mucosa and a preparation method thereof, and belongs to the field of medical technology. The drug includes barnacle peptide, salivaricin, resveratrol, a biphasic adhesive matrix, hydroxyapatite, vitamin B group and other components. Gooseneck barnacles are enzymatically hydrolyzed by pepsin and lumbrokinase in sequence to obtain barnacle peptide. The biphasic adhesive matrix includes an aqueous phase matrix, an oil phase matrix and lecithin; the aqueous phase matrix contains chondroitin sulfate-chitosan quaternary ammonium salt complex and sodium alginate and other components; the oil phase matrix includes sesame oil, beeswax, polylactic acid-glycolic acid copolymer and other components. The present invention adopts a special method to prepare barnacle peptide and salivaricin, and the use of them in combination with resveratrol can achieve multi-target and multi-pathway promotion of oral mucosa repair; the chondroitin sulfate-chitosan quaternary ammonium salt complex is prepared by electrostatic and physical encapsulation, and the biphasic adhesive matrix is ​​prepared in combination with other components, which has good biocompatibility, strong adhesion, and can well prolong the action time of sustained-release drugs.

The invention discloses a high-acid-resistance lumbrukinase enteric-coated pellet and a preparation method thereof, and relates to the technical field of pharmacy. The preparation method comprises the following steps: S1, mixing lumbrukinase dry powder with blank mixed powder to obtain lumbrukinase mixed powder; s2, blank pellets with the particle size of 900-1400 microns, an adhesive and lumbrukinase mixed powder are mixed, medicine wrapping and drying are conducted, and medicine-containing pellets are obtained; s3, the drug-containing pellets are subjected to enteric coating, the moisture content of the coated pellets is smaller than or equal to 8%, and the drug-containing pellets are obtained. By controlling the particle size and the moisture after coating, the acid resistance of the prepared lumbrukinase is larger than 97% and is still higher than 90% after being accelerated for 6 months, the stability is remarkably improved, and the titer of the lumbrukinase is more stable.