Detection method by using PRSS2 gene as lactoprotein content molecular marker

Abstract

The invention discloses a detection method by using a PRSS2 gene as a lactoprotein content molecular marker. The method comprises the following steps: 1. carrying out whole genome DNA (deoxyribonucleic acid) extraction on bovine blood, and inspecting the DNA quality by agarose gel electrophoresis; 2. carrying out PCR (polymerase chain reaction) amplification on the bovine PRSS2 gene segment by using the whole genome DNA as a template and a primer A as the primer; 3. carrying out mixed-cell PCR on the whole genome DNA to detect the polymorphism sites; and 4. carrying out sequencing on an individual sample, and carrying out polymorphism correlation analysis according to the sequencing result. The method is utilized to detect the relevance between 5 SNP (single-nucleotide polymorphism) sites and lactoprotein traits in the bovine milk quality. The invention provides a simple quick genetic marker for screening and detecting close relevance to bovine milk quality traits on the DNA level, and the genetic marker can be used for bovine molecular breeding. The method solves the problem of high complexity in the SSCP (single strand conformation polymorphism) and PCR-PFLP (polymerase chain reaction-restriction fragment length polymorphism) techniques, and enhances the accuracy and precision.

Description

technical field [0001] The invention relates to a detection method of a milk protein content molecular marker, in particular to a detection method using PRSS2 gene as a milk protein content molecular marker. Background technique [0002] Single Nucleotide Polymorphism (SNP) refers to the variation of a single nucleotide on the genome—A, T, C or G variation, which causes a change in the DNA sequence, resulting in polymorphism of the chromosomal genome between different species . The genetic markers formed by the single nucleotide variation (including substitution, transversion, deletion, and insertion) on the genome are large in number and rich in polymorphisms. In general, SNP refers to a single nucleotide variation with a variation frequency greater than 1%. Any base in genomic DNA may vary, so SNP may be in the gene sequence, that is, in the coding region, or in the non-coding sequence. However, there are relatively few cSNPs (codingSNP) in the coding region, and its va...

AI Technical Summary

Problems solved by technology

Therefore, the study of the function and regulation mechanism of this gene has become a hotspot in genetics research, and then the study of the PRSS2 gene has important significance. At present, there are relatively few studies on it in dairy cows, and the relationship between PRSS2 gene and dairy cow traits The correlation of

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