Detection method using prss2 gene as molecular marker of milk protein content
A technology of molecular markers and detection methods, which is applied in the direction of biochemical equipment and methods, microbial measurement/inspection, etc., to achieve the effect of improving accuracy and precision
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[0034] 1. Test materials and methods:
[0035] 1 Experimental materials:
[0036] 1.1 Test animals:
[0037] Taking the Chinese Holstein dairy cow population as the test object, the cows in a large dairy farm in Liaoning Province were selected, and the samples were collected by jugular vein blood sampling.
[0038] 1.2 Test reagents:
[0039]Agarose (BIOWEST); TAE and diethyl pyrophosphate (DEPC) were purchased from Sigma Company; PCR primers were synthesized by Shanghai Bioengineering Company; DNA extraction kit and 2XTaq PCR Master Mix were purchased from TIANGEN Company.
[0040] 1.3 DNA analysis software:
[0041] PCR primer design software: Primer Premier 5;
[0042] Data analysis software: SPSS 13.0;
[0043] 2. Experimental method:
[0044] In this experiment, DNA extraction kit from TIANGEN Company was used to extract the whole genome DNA from the blood of Chinese Holstein cows. At the same time, primers were designed for PCR amplification, and the products were ...
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