Application of ANGPTL5 gene as bovine backfat thickness molecular marker and detection method
A technology of molecular markers and detection methods, which is applied in the direction of biochemical equipment and methods, microbial measurement/inspection, etc., can solve the problem of less research on the function of the bovine ANGPTL5 gene, and achieve the purpose of improving population quality, reducing costs, improving accuracy and The effect of precision
Active Publication Date: 2019-09-20
JILIN UNIV
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Problems solved by technology
However, there are few studies on the function of bovine ANGPTL5 gene
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Embodiment 1
[0035] 1. Materials and methods
[0036] 1.1 Experimental materials
[0037] Blood DNA samples of Chinese Simmental cattle (28-month-old, bulls) were obtained from the slaughterhouse of Baolongshan Cattle Farm in Inner Mongolia.
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The invention discloses application of ANGPTL5 gene as a bovine backfat thickness molecular marker and a detection method. After bovine genome-wide DNA is subjected to PCR (polymerase chain reaction) amplification, SNPs (single nucleotide polymorphisms) present in a PCR product sequence are detected through direct sequencing; it is discovered that four SNPs on an intron 1 and an intron 2 in the ANGPTL5 gene sequence are associated with bovine backfat thickness traits. A genetic marker in tight relation with bovine backfat thickness traits is screened and detected effectively, quickly and simply at DNA level; the genetic marker is applicable to molecular breeding of beef cattle; in initial stage of cattle breeding, the functional SNPs are used as markers for molecular breeding and assisted selection for modifying backfat thickness traits of Chinese Simmental cattle; bovine backfat thickness can be assessed and predicted quickly; therefore, with the cost reduced, beef quality is further improved, breeding speed of fine varieties is increased, and population quality is improved.
Description
technical field [0001] The present invention discloses the use of ANGPTL5 gene as a molecular marker of bovine backfat thickness, and also provides a detection method, which relates to a single nucleotide polymorphism of ANGPTL5 gene related to the trait of backfat thickness of Chinese Simmental cattle and its detection method and applications, belonging to the field of biotechnology. Background technique [0002] With the development of beef cattle industry and economy, consumers have higher and higher requirements for beef quality and taste. Therefore, meat quality traits and carcass traits have attracted much attention in the research of cattle breeding and trait improvement. Meat quality traits include eye muscle area, fat color, marbling score, and carcass traits include carcass weight, carcass length, carcass depth, slaughter rate, slaughter pH, net bone weight, backfat thickness, etc. Among them, the backfat thickness is the thickness of the back meat between the 12t...
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IPC IPC(8): C12Q1/6888C12Q1/6858
CPCC12Q1/6888C12Q1/6858C12Q2600/124C12Q2600/156C12Q2531/113C12Q2535/122
Inventor 房希碧赵志辉杨润军葛延亮夏立新
Owner JILIN UNIV
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