Method for detecting bovine fat traits by utilizing SDC3 promoter genetic marker

A marker detection, SDC3-S technology, applied in the direction of biochemical equipment and methods, microbial measurement/inspection, etc., can solve the problems of few reports, improve the quality of the population, speed up the breeding of improved varieties, improve the accuracy and The effect of precision

A marker detection, SDC3-S technology, applied in the direction of biochemical equipment and methods, microbial measurement/inspection, etc., can solve the problems of few reports, improve the quality of the population, speed up the breeding of improved varieties, improve the accuracy and The effect of precision

CN111118180AActive Publication Date: 2020-05-08JILIN UNIV
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  • Method for detecting bovine fat traits by utilizing SDC3 promoter genetic marker
  • Method for detecting bovine fat traits by utilizing SDC3 promoter genetic marker
  • Method for detecting bovine fat traits by utilizing SDC3 promoter genetic marker

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] 1. Experimental materials and equipment

[0040] 2×Taq PCR Green Mix (Novizyme, Nanjing), ethidium bromide substitute (Dingguochangsheng, Beijing), DL2000DNA Marker (TaKaRa, Dalian), agarose (Biowest Agarose), 50×TAE (Solaibao ).

[0041]PCR instrument (BIO-RAD Company); electrophoresis instrument (Shanghai Junyi Co., Ltd.); high-speed centrifuge (Shanghai Chemical Machinery Factory Co., Ltd.); -20 ℃ refrigerator (Haier); PCR tube (Axygen Company); gel imaging system (UVC);

[0042] 2. The main software used in the experiment

[0043] Sequencing results data analysis software SeqMan and statistical analysis software SPSS 23.0, etc. The linkage relationship and haplotype of 10 single nucleotide polymorphisms were analyzed using HaploView software;

[0044] 3. Experimental method

[0045] The blood of Chinese Simmental bulls was provided by Baolong Shanniu Farm in Inner Mongolia. Genomic DNA was extracted using a whole blood DNA extraction kit, and the DNA of each in...

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Abstract

The invention discloses a method for detecting bovine fat traits by utilizing an SDC3 promoter genetic marker. By carrying out PCR amplification on whole genome DNA of cattle and by detecting SNPs existing in a PCR product sequence by DNA sequencing, the invention discloses the correlation between seven SNPs sites on an SDC3 gene promoter region and bovine fat traits; and the method for predictingand breeding marbling grade, fat coverage rate and fat color character of beef cattle by utilizing the SDC3 gene promoter region marker lays a foundation for early breeding of genetic workers, improvement of meat quality and further improvement of the economic benefit of beef cattle breeding.

Description

Technical field: [0001] The invention discloses a method for detecting bovine fat traits by using SDC3 promoter genetic markers, in particular to a method for predicting bovine fat coverage, marbling, and fat color traits by detecting SNPs sites and haplotypes in the promoter region of SDC3 gene The molecular marker detection method and its application for evaluation and evaluation belong to the technical field of breeding prediction and breeding beef quality traits. Background technique: [0002] In the field of beef cattle feeding technology, the functional genes and molecular markers related to cattle fat traits are of great significance and application value especially for the early screening and breed improvement of beef cattle. [0003] Single nucleotide polymorphisms (SNPs) mainly refer to DNA sequence polymorphisms caused by single nucleotide variation at the genome level. The polymorphism represented by SNP only involves the variation of a single base, which can be...

Claims

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Application Information

Patent Timeline
08 May 2020
Publication
CN111118180A
IPC
C12Q1/6888
CPC
C12Q1/6888; C12Q2600/124; C12Q2600/156; C12Q2600/172
Inventors
白紫彤; 房希碧