13 results about "DNA sequencing" patented technology

The present disclosure provides flow cell devices, systems, and methods for facilitating and performing DNA sequencing analysis with reduced system complexity and cost, significant cost of goods saving, and reduced contamination level. The sequencing systems described herein permit processing of multiple flow cells simultaneously, such that sequencing and imaging steps, or multiple sequencing methods, can be performed in parallel using a single sequencing system.

The present application relates to the technical field of fluorescent dyes, in particular to an asymmetric pentamethine cyanine compound. The compound comprises a structure as shown in formula I. The compound provided by the present application has good water solubility, near-infrared absorption, high fluorescence quantum yield and good molar absorption coefficient, and can be used in nucleic acid labeling, DNA sequencing, cell imaging, protein labeling or specific recognition of amino acids.

This invention discloses a method for calculating a polygenic risk score (PRS or PRS score) for predicting autism and neurodevelopmental disorders (hereinafter collectively referred to as diseases). Its key feature is the improvement in the accuracy of predicting the relative risk of potential diseases by incorporating the genetic effects of rare variants (MAF < 0.1%) and common variants (MAF > 0.1%). This invention uses loss-of-function (LoF) rare variants detected by DNA sequencing (including but not limited to NGS / 3GS) to calculate gene-based PRS scores; and integrates these scores into existing snp-based PRS scores based solely on common variants detected by GWAS; thereby solving the problem of low variance interpretation (pseudo-R) in snp-based PRS scores calculated only based on common variants. 2 (and the problem of low predictive ability.)

Methods comprising obtaining a biological sample and extracting nucleic acids from it are disclosed. The methods process the nucleic acids for DNA sequencing and identify in the DNA sequencing data a plurality of amplicon sequence variants (ASV). The methods record the relative abundance of each ASV and classify, using a machine learning algorithm, each ASV according to one of a set of taxonomies. The set of taxonomies includes a pathobiont bacteria subset consisting of genus level groups Eggerthella group, Ruminococcus torques group, Ruminococcus gnavus group, Coprobacillus group, Streptococcus group, Bilophila group, Actinomyces group, Desulfovibrio group, Atopobiaceae group, Veillonella group, Enterococcus group, Escherichia-Shigella group, Campylobacter group, Enterobacteriaceae group, and Fusobacterium group. Based on the relative abundance of the ASV classified as pathobiont bacteria, a numerical indication of the health of the subject is calculated. Based on the numerical indication, the methods deliver to the subject a customized intervention.

The application discloses a hydrogel crosslinking agent for gene sequencing and a synthesis method thereof. The crosslinking agent is used for synthesizing a hydrogel polymer material with a space network structure, and the material is applied to a carrier of a reaction system of DNA sequencing of enzymes and substrates.

The present invention relates to computer-implemented methods for identifying a DNA modification in DNA sequencing data for the intraoperative classification of a disease and / or disorder, to methods for identifying a disease and / or disorder, to methods of the pre-operative configuring of a DNA modification-based DL-classifier and to methods of treatment and surgery.

This application discloses a bioinformatics analysis method and apparatus based on deaminase-treated single-stranded DNA sequencing data. The method determines a first sequencing sequence set and a second sequencing sequence set for a target cell. The first sequencing sequence set includes multiple first sequencing sequences obtained by sequencing deaminated DNA, and the second sequencing sequence set includes multiple second sequencing sequences obtained by sequencing untreated DNA. The method determines the number of detections and transitions for each site in each first and second sequencing sequence. Based on the number of detections and transitions for each site in each second sequencing sequence, the method determines the global background error rate. Based on the global background error rate and the number of detections and transitions for each site in each first sequencing sequence, the method determines at least one target site. Peak calling and clustering are performed on the first sequencing sequences including at least one target site to obtain single-stranded DNA regions in the target cell.

Provided are an imaging device performing DNA sequencing imaging and an operating method thereof. The imaging device for imaging a DNA signal may: shift, to a unit pixel pitch or less of an image sensor, a position at which emission light emitted from a DNA sample irradiated with a beam is focused on the image sensor; acquire a plurality of images by using the image sensor to receive emission light that is focused at the shifted position on the image sensor; and synthesizing the plurality of acquired images to acquire a result image.

The application discloses the technical field of petroleum geology engineering and specifically provides a microbial DNA sequencing analysis method and application thereof in formation division, which comprises the following steps: during drilling, sampling cuttings from a shaker at a wellhead, and collecting a cuttings sample every 0.5 meters to collect cuttings samples at different formation depths; treating the collected cuttings and performing DNA sequencing to obtain the types and abundance of bacteria contained in the cuttings; preliminarily screening the bacteria, and retaining bacteria from an underground oil reservoir; clustering the retained bacteria, and preliminarily dividing the total length of the formation from which the cuttings are collected into multiple formations according to the similarity and correlation of the bacteria at different formation depths; and finely dividing each formation into multiple small layers by taking characteristic bacteria in each formation as main characteristics.