17 results about "Genomic screening" patented technology

This invention discloses a method for rapidly detecting conventional and hybrid varieties of industrial chili peppers and its application, relating to the field of molecular breeding technology. The method includes: extracting DNA from fresh leaves of the industrial chili pepper to be tested and performing whole-genome resequencing at a sequencing depth of 8×–12×; aligning the sequencing data to the chili pepper reference genome (Capsicum chinense cv. 'PBC932') to screen for high-quality heterozygous SNP loci; using a 1 Mb sliding window to statistically analyze the distribution density of heterozygous SNPs across the entire genome; and determining the variety type based on density thresholds: an average density <300 SNPs / Mb indicates a conventional variety, and >2000 SNPs / Mb indicates a hybrid variety. This invention eliminates the need for phenotypic observation in subsequent generations, requiring only leaves from current-generation plants to rapidly and accurately distinguish conventional and hybrid varieties at the molecular level. It features a short detection cycle, objective and reliable results, and is not limited by the number of molecular markers, providing strong technical support for efficient identification and early screening of industrial chili pepper germplasm resources.

The invention discloses a method for identifying the purity of a coconut strain. The method comprises the following steps: collecting DNA of a target sample; constructing a DNA library of the target sample, and performing whole genome re-sequencing; filtering data in the DNA library to obtain an impurity-removed DNA sequence; comparing the DNA sequence subjected to impurity removal with a reference genome, carrying out variation detection, generating SNP and INDEL marker files, then carrying out genetic typing, and constructing a variety identification model; comparing and analyzing whole genome SNPs of the two groups, and screening specific sites capable of identifying and distinguishing the two groups in the genome; and inputting the DNA of the target sample into the variety identification model, detecting the SNP specific site of the gene, and judging the variety result to realize the identification of the purity of the coconut variety. The identification accuracy of the identification method can reach 99% and is far higher than that of a traditional morphological method, rapid identification can be conducted in the seedling stage, and the identification period is greatly shortened.

The invention discloses application of a molecular target Ptchd1 in nerve synaptic function and consciousness regulation and related diseases. Whole genome screening finds that Ptchd1 is a key molecular target for consciousness regulation, and animal experiments prove that the target has an important regulation effect on neuronal synaptic vesicle release and consciousness disappearance caused by general anesthesia drugs. In view of the fact that Ptchd1 can regulate and control drug-induced awareness disappearance and neuronal synaptic delivery, related treatment with Ptchd1 as a target spot has significant potential values in the following fields: i) application in preparation of reagents for regulating and controlling a neuronal synaptic function and awareness regulation and control; the invention also relates to application of the pharmaceutical composition in preparation of drugs for promoting awakening after anesthesia and application of the pharmaceutical composition in preparation of drugs for promoting awakening of diseases related to disturbance of consciousness, wherein the diseases related to the disturbance of consciousness comprise vegetative people, micro-consciousness or brain injury coma.

The invention relates to the technical field of biology, in particular to a primer group for identifying Hangzhou pig breeds, a detection method and application. Four specific SNP molecular markers SNP1-SNP4 are obtained through whole genome screening, the upstream and downstream sequences of the molecular markers are shown as SEQ ID NO.1-4, four pairs of specific PCR primers are designed and developed based on the sites, and the primer group and the detection method are applied to the identification of Hangzhou pig breeds. The nucleotide sequence of the gene is as shown in SEQ ID NO.5-12. And when the genotypes of the to-be-detected sample at the SNP1-SNP4 sites are CC, CC, TT and CC respectively, determining that the to-be-detected sample is a Hangzhou pig purebred individual. Compared with a traditional phenotype identification method, the molecular marker detection system established by the invention has the advantages of high detection efficiency, good accuracy and flux. The technical scheme provided by the invention provides important technical support for germplasm resource protection, variety purity identification and genetic improvement of Hangzhou pigs, and has remarkable application value in the field of livestock breeding.

The invention provides compositions and methods for performing mammalian cell genetics, e.g., genetic screens, using near-haploid cells. The invention further provides genes and gene products isolated using the inventive methods and methods of use thereof.

The invention relates to pig variety molecular identification, and discloses an SNP molecular marker combination for identifying Wanyue black pigs, the combination is composed of the following 27 SNP sites, the physical position and allele information of each SNP site are determined based on a pig reference genome Sscrofa11.1: rs328711913 located at the 8482083bp position of a chromosome 1, the allele of which is G / C, and rs328711913 located at the 8482083bp position of a chromosome 2, and the allele of which is G / C; the rs81310649 is located at the 9002974bp position of the chromosome 1, and the allele of the rs81310649 is T / C; and the rs340136088 is located at the 91975324bp position of the chromosome 2, and the allele of the rs340136088 is G / A. According to the invention, an identification marker set composed of 27 specific SNPs is determined through whole genome screening, and the uniform REF / ALT unification, 0 / 1 / 2 coding, mu / sigma standardization and threshold determination rules are matched, so that the detection process is clear and repeatable, and uniform operation and result interpretation in actual detection are facilitated.

The invention discloses a bird and animal keratinized tissue mitochondrial DNA assembly method. The method comprises the following steps: acquiring second-generation sequencing data and cutting a low-quality sequence to generate a clean file; carrying out comparison until a mitochondrial reference genome is obtained, and screening a homologous sequence, so as to obtain unclassifid.sam; analyzing terminal base damage, counting significant damage and determining a parameter N; the SAM is converted into a mapped.fq.gz file; generating a pre-processing file based on N-substituted forward data 5'end front N basic groups and reverse data 3 'end front N basic groups; and iteratively assembling and outputting a complete mitochondrial DNA sequence by using a preset k value, thereby improving the speed and integrity.

The invention discloses a specific SNP (Single Nucleotide Polymorphism) molecular marker for identifying euchiloglanis irchiloglanis and an identification method, according to the method, the whole mitochondrial genome of euchiloglanis irchiloglanis and the mitochondrial genome of a near-margin euchiloglanis fish are compared, a specific region of an ND2 gene is screened as the specific SNP molecular marker, and the nucleotide sequence of the specific SNP molecular marker is shown as SEQ ID NO.1. The specific primer pair is utilized to perform PCR amplification and Sanger sequencing on a to-be-detected sample, and accurate identification is realized by comparing basic groups of key SNP sites contained in the molecular marker. The method has the advantages of high specificity, high accuracy and the like, and is suitable for germplasm resource identification and protection of euchiloglanis longischii.

The invention discloses a method for screening soil oxytetracycline high-efficiency degrading bacteria by utilizing metagenomes, which comprises the following steps of: selecting typical soil types, detecting soil oxytetracycline, determining the metagenomes of soil microorganisms, and screening dominant bacterial communities in oxytetracycline-polluted soil, so that the method for screening the oxytetracycline high-efficiency degrading bacteria is established. The test soil provided by the invention is blue and purple clay and black soil, and is a typical farmland soil type in south and north. The method is high in efficiency and simple and convenient in experiment, can be used for rapidly screening the antibiotic degrading flora, and is of great significance in breaking through the bottleneck of a traditional screening technology and rapidly obtaining an efficient degrading strain.

The invention discloses a molecular marker-assisted procypris merus-crucian carp hybrid purity identification method, and relates to the technical field of freshwater fish hybridization. According to the method, objectification and standardization of the identification process are achieved, purity identification is converted into objective data analysis from experience-dependent morphological observation by using a high-quality SNP marker screened by a whole genome and a clear mathematical judgment algorithm, the result is accurate, reliable and repeatable, and the method has the advantages of high throughput, high efficiency, high sensitivity and the like. On the basis of a high-throughput genotyping technology of multiple PCR or gene chips, dozens of or even hundreds of markers can be detected in parallel at a time, and by combining with an automatic analysis system, identification of a large batch of samples can be completed in a short time, so that the breeding screening efficiency is greatly improved.

The invention discloses a molecular marker for identifying euchiloglanis kishinouyei and a method for identifying the euchiloglanis kishinouyei. According to the method, mitochondrial whole genome of euchiloglanis kishinouyei and mitochondrial whole genome of euchiloglanis kishinouyei fishes close to the euchiloglanis kishinouyei are compared, an ATP6 gene specific region is screened out to serve as a specific molecular marker of interspecific stable difference, and the nucleotide sequence of the specific molecular marker is shown as SEQ ID The method comprises the steps of DNA (deoxyribonucleic acid) extraction of a sample to be detected, PCR (polymerase chain reaction) amplification, electrophoresis detection, Sanger sequencing and species identification, and the euchiloglanis kishinouyei can be quickly and accurately identified. The method is easy and convenient to operate, high in accuracy and high in specificity, and has wide application prospects in the fields of fish taxonomy research, fishery resource investigation and the like.

This invention relates to a primer and probe combination, kit, and application for the visual detection of LayV (Layvirus RT-RPA), belonging to the field of nucleic acid visualization detection technology. To address the technical problems of LayV detection methods, such as cumbersome processes, long processing times, latency, high requirements for operating environments and precision equipment, and the inability to achieve rapid on-site detection of LayV, this invention compares the LayV genome, screens multiple highly conserved gene sequence regions, and ultimately identifies the conserved region of the LayV N protein gene as the detection target. A primer and probe combination for the visual detection of LayV using RT-RPA was designed, and a corresponding detection method was obtained. The detection method provided by this invention has high sensitivity and specificity, enabling rapid and efficient detection of LayV without requiring special instruments or professional technicians. It is simple to operate and provides a powerful tool for rapid on-site diagnosis, monitoring, and epidemiological investigation of LayV.

This invention discloses a strain of Bacillus subtilis, its screening method, and its application. The Bacillus subtilis strain is named... Bacillus subtilis BS61, deposited on September 4, 2025, at the China General Microbiological Culture Collection Center (CGMCC), accession number: CGMCC No. 35815. The screening method included phenotypic and genomic screening. Phenotypic screening tested the strain's resistance to acid, bile salts, heat, and artificial gastrointestinal fluids. It revealed that strain BS61 exhibited significantly better antibacterial effects against various pathogens, including *Escherichia coli* K88 and *Salmonella typhimurium*, compared to the standard strain *Bacillus subtilis* BS168. It contains antibacterial secondary metabolic gene clusters such as Fengycin, Thurincin H, and Bacillibactin, but lacks aggressive virulence factors and contains only three low-abundance drug resistance genes. This invention can be applied to the preparation of livestock and poultry feed additives, with no risk of widespread drug resistance. Its use does not affect animal health or food safety, and it can replace antibiotics, improving the intestinal health of livestock and poultry.

The application belongs to the technical field of biotechnology, and particularly relates to a recombinant vector, a cell, a kit and a method for screening of a host factor dependent on influenza virus polymerase activity. The application provides a recombinant vector, which is configured with an mScarlet reporter gene through a double tandem strategy (PB1-PB2, PA-NP), and a red fluorescent cell line stably expressing H5N1 polymerase driven by the red fluorescent cell line can be efficiently constructed by using only three lentiviral vectors. Compared with a traditional co-transduction method of five vectors, the difficulty of multiple vectors entering the same cell is greatly reduced, and the recombinant vector does not need live virus and can be stably passaged for a long time. Based on the recombinant vector, a host factor affecting polymerase activity is screened by using a whole genome CRISPR under a non-infectious state, and the method has the advantages of simple operation, high throughput and low cost.

The invention discloses an application of Pitavastatin calcium in preparation of a medicine for targeting TRAAPPC4 (TRAPPC4) so as to enhance ferroptosis sensitivity and treat head and neck squamous cell carcinoma. Through whole genome CRISPR / Cas9 screening, it is found that TRAPPC4 is a key factor of ferroptosis resistance in HNSCC. Through virtual screening and experimental verification, it is identified that the Pitavastatin calcimum is an inhibitor of the TRAPPC4. The Pitavastatin calcium and the RSL3 are combined for use, so that the ferroptosis effect can be synergistically enhanced, and the tumor growth can be remarkably inhibited. The invention provides a novel medicine application of Pitavastatin calcium, namely the Pitavastatin calcium is used for preparing a medicine or a medicine composition which is targeted to TRAPPC4 and is used for treating head and neck squamous cell carcinoma.

This invention discloses SNP molecular markers simultaneously associated with oil and protein content in upland cotton seed kernels and their applications. The SNP molecular markers are at least one of the nucleotide sequences shown in SEQ ID NO.1-SEQ ID NO.57. The SNP molecular markers related to oil and protein content in upland cotton seed kernels provided by this invention can be used for early prediction and screening of these traits. These molecular markers exist in DNA form, can be detected in various tissues and developmental stages of cotton, are unaffected by season or environment, and do not involve expression issues. These markers have no effect on the expression of the target trait, exhibiting neutrality. In genome screening, typically only + / - analysis is required, without analyzing fragment length, which facilitates the development of automated technologies for SNP screening or detection. Validation experimental results show that all SNP sites screened by this invention have a significant impact on the variation of oil and protein content traits in upland cotton seed kernels.

The invention discloses deoxycytidine monophosphate hydroxylase AfhB and application of the deoxycytidine monophosphate hydroxylase AfhB. The dCMP hydroxylase AfhB obtained by screening from the genome of the bacteriophage HY126 can be used for green synthesis or fermentation production of 5hdCMP, so that the environmental pollution caused by a traditional chemical synthesis method is greatly reduced, and a new enzyme element and way are provided for development of a gene editing technology and fermentation production of 5hdCMP and derivatives thereof in industrial fermentation.