6results about How to "Improve fermentation titer" patented technology

The invention provides a method for increasing the fermentation unit of acarbose. The method mainly comprises the step of adding metal ions into a fermentation culture medium. According to the method, metal ions such as copper sulfate, zinc sulfate or manganese sulfate and the like are added into the fermentation culture medium, and ferric chloride is added in a matched manner, so that the fermentation titer of acarbose is increased to 8.83 mu g / mL under the condition of not changing strains and the fermentation culture medium, and the fermentation unit of acarbose is remarkably increased; in addition, the fermentation period is shortened, and the production cost is reduced.

The application discloses a spiramycin fermentation method based on nitrogen source regulation, and relates to the technical field of spiramycin fermentation. The application improves the spiramycin fermentation titer and reduces the generation levels of impurities D and F without changing the production strain, introducing complex genetic modification and large equipment modification, by adjusting the ratio of the available inorganic nitrogen source and the composite organic nitrogen source in the initial culture medium, and optimizing the composition of the organic nitrogen source in the feeding stage. The application can solve the problems in the existing mature fermentation process, such as the limited titer improvement space, the possible influence of the slow-release organic nitrogen source such as soybean meal on impurity F in the feeding stage, the mismatch between the nitrogen metabolism rhythm and the secondary metabolism start caused by the too fast use of the available ammonium salt, and the high impurity D level in the late fermentation stage. The application also verifies the action mechanism of the nitrogen source regulation process by the changes of the total sugar, amino nitrogen and related biosynthesis gene expression in the fermentation process, and provides a basis for subsequent process amplification and quality control.

The invention belongs to the technical field of biological medicines, and discloses a method for preparing clavulanic acid by a biological fermentation method. The method comprises the following steps: inoculating a seed bacterial liquid of streptomyces clavuliformis into a fermentation culture medium for fermentation culture, and collecting a fermentation product to obtain clavulanic acid, wherein in the fermentation culture process, an amylase enzymolysis solution is supplemented, the total sugar concentration range of fermentation liquor is maintained to be 6 g / L-12 g / L, and the amylase enzymolysis solution is obtained by performing enzymolysis and liquefaction on starch through alpha-amylase. By adopting the method, the fermentation titer can be improved, and the production cost of the clavulanic acid can be reduced.

The invention provides riemerella anatipestifer bacteriophage as well as a separation and purification method and application thereof. The riemerella anatipestifer phage is obtained through the steps of enrichment, separation, purification and the like, the phage is named as the riemerella anatipestifer phage vBRieM001 (Riemerella anatipestifer phage vBRieM001), the riemerella anatipestifer phage vBRieM001 is preserved in the China Center for Type Culture Collection (CCTCC), and the preservation number is CCTCC NO: M20221952. Experiments such as optimal infection complex number, one-step growth curve, pH tolerance, temperature sensitivity and lysis spectrum determination are carried out on the bacteriophage, and finally, the bacteriophage has wide lysis spectrum, strong adaptability to environment, strong stress resistance and good tolerance to temperature and pH, and the lysis rate of riemerella anatipestifer reaches up to 78.57%.

The invention relates to the technical field of microbial fermentation, and discloses a genetically engineered bacterium for improving the titer of abamectin, a construction method and application of the genetically engineered bacterium. According to the invention, the ftsZ gene or / and the overexpression mreB12 gene are / is knocked out, so that the mycelial morphology of the streptomyces avermitilis is regulated and controlled, the growth and development of the mycelia are more coordinated, the appearances of the mycelia and spores are smoother, the production of the abamectin is facilitated, the storage of more metabolites is facilitated, and the yield of the abamectin is obviously increased. When the ftsZ gene is knocked out, the fermentation titer of the streptomyces avermitilis can be improved by 94.74%, when the mreB12 gene is overexpressed, the fermentation titer of the streptomyces avermitilis can be improved by 139.47%, and when the ftsZ gene is knocked out and the mreB12 gene is overexpressed at the same time, the fermentation titer of the streptomyces avermitilis can be improved by 157.89%. The method disclosed by the invention has great reference significance for producing abamectin by utilizing the streptomyces avermitilis in industrial fermentation.

The present application relates to a kind of fermentation medium of mupirocin and the production method of mupirocin.The present application improves fermentation medium and the production method of mupirocin, significantly improves the fermentation level of mupirocin, and when fermenting 5-7 days, the fermentation titer reaches 12000mg / L or more.The whole production process is cheap and easy to obtain, and the fermentation process is simple and easy to operate, which is beneficial to industrial production.