3results about How to "Improve fermentation titer" patented technology

The application discloses a spiramycin fermentation method based on nitrogen source regulation, and relates to the technical field of spiramycin fermentation. The application improves the spiramycin fermentation titer and reduces the generation levels of impurities D and F without changing the production strain, introducing complex genetic modification and large equipment modification, by adjusting the ratio of the available inorganic nitrogen source and the composite organic nitrogen source in the initial culture medium, and optimizing the composition of the organic nitrogen source in the feeding stage. The application can solve the problems in the existing mature fermentation process, such as the limited titer improvement space, the possible influence of the slow-release organic nitrogen source such as soybean meal on impurity F in the feeding stage, the mismatch between the nitrogen metabolism rhythm and the secondary metabolism start caused by the too fast use of the available ammonium salt, and the high impurity D level in the late fermentation stage. The application also verifies the action mechanism of the nitrogen source regulation process by the changes of the total sugar, amino nitrogen and related biosynthesis gene expression in the fermentation process, and provides a basis for subsequent process amplification and quality control.

The invention provides riemerella anatipestifer bacteriophage as well as a separation and purification method and application thereof. The riemerella anatipestifer phage is obtained through the steps of enrichment, separation, purification and the like, the phage is named as the riemerella anatipestifer phage vBRieM001 (Riemerella anatipestifer phage vBRieM001), the riemerella anatipestifer phage vBRieM001 is preserved in the China Center for Type Culture Collection (CCTCC), and the preservation number is CCTCC NO: M20221952. Experiments such as optimal infection complex number, one-step growth curve, pH tolerance, temperature sensitivity and lysis spectrum determination are carried out on the bacteriophage, and finally, the bacteriophage has wide lysis spectrum, strong adaptability to environment, strong stress resistance and good tolerance to temperature and pH, and the lysis rate of riemerella anatipestifer reaches up to 78.57%.

The present application relates to a kind of fermentation medium of mupirocin and the production method of mupirocin.The present application improves fermentation medium and the production method of mupirocin, significantly improves the fermentation level of mupirocin, and when fermenting 5-7 days, the fermentation titer reaches 12000mg / L or more.The whole production process is cheap and easy to obtain, and the fermentation process is simple and easy to operate, which is beneficial to industrial production.