Method for fermentation production of lipstatin and culture medium components thereof

Abstract

The invention provides a method for fermentation production of lipstatin, belonging to the field of fermentation production of microorganisms. The method takes streptomyces toxytricini as an initial bacterium and mainly comprises the steps of preparation of a slope strain, preparation of a seed solution, control of a fermentation process and the like. In the invention, by taking soybean meal, bean oil and glycerol as main nitrogen sources and carbon sources of a fermentation culture medium and regulating and controlling the fermentation process and the components of the culture medium, the production cost of the lipstatin is greatly lowered, and the fermentation titer is increased, thus the defects of high production cost and low fermentation titer in the prior art are overcome.

Description

technical field

[0001] The invention relates to the field of microbial fermentation, in particular to a method for fermenting and producing riprestatin and its culture medium components. Background technique

[0002] The liprestatin produced by the fermentation method involved in the present invention is an intermediate of the novel weight-loss drug orlistat. Orlistat is currently the only weight-loss drug in the world. It is a long-acting and potent specific gastrointestinal lipase inhibitor. The covalent bond inactivates the enzyme to play a therapeutic role. The inactivated enzyme cannot hydrolyze the fat in food, mainly triglyceride, into absorbable free fatty acid and monoacylglycerol. Undigested triglycerides cannot be absorbed by the body, thereby reducing caloric intake and controlling weight.

[0003] There are two methods for the production of orlistat, fermentation synthesis and total synthesis. The production cost of the total synthesis method is relatively hi...