11 results about "Mycophenolic acid" patented technology

The application discloses a kind of rice mycotic acid hapten and antigen preparation application, it is related to antigen preparation technical field, introduce sulfydryl in the 2 of rice mycotic acid, and obtain rice mycotic acid antigen by coupling with protein through connecting arm, rice mycotic acid hapten and antigen preparation application include the following specific flow: S1, the synthesis of rice mycotic acid hapten;S2, the preparation of rice mycotic acid immunogen;S3, the preparation of rice mycotic acid coating original;S4, the preparation of rice mycotic acid monoclonal antibody;S5, the preparation of rice mycotic acid immunofluorescence detection card;S6, rice mycotic acid detection in food;S7, detection limit experiment;S8, false positive rate, false negative rate experiment.The rice mycotic acid hapten retains the structural characteristics of rice mycotic acid, better exposes rice mycotic acid structural characteristics, improves the immunity and immune recognition ability of antigen, lays foundation for preparing specific, high sensitivity antibody, and improves its rapid detection ability.

The application belongs to the field of medicine and chemical industry, and discloses a mycophenolic acid medicine co-crystal and a preparation and application thereof. The co-crystal is prepared from mycophenolic acid and 4,4-bipyridine as raw materials. The co-crystal is a stoichiometric ratio diversity co-crystal. In the molecular structure of the co-crystal, the amount of substance of mycophenolic acid and 4,4-bipyridine is 1:1 or 2:1. The two kinds of mycophenolic acid medicine co-crystals prepared by a grinding method, a room temperature evaporation method or a slurry method are diversity co-crystals, and the diversity of the mycophenolic acid co-crystal has never been reported. The application solves the problems of poor stability and high hygroscopicity of mycophenolic acid, and the prepared co-crystal has low hygroscopicity and good stability.

The present application relates to a kind of mycophenolic acid detection reagent and its preparation and detection method, specifically a kind of mycophenolic acid chemiluminescence immunoassay reagent and its preparation and detection method, comprising: anti mycophenolic acid specific antibody, for detecting the indicating reagent of anti mycophenolic acid specific antibody-mycophenolic acid combination;The above anti mycophenolic acid specific antibody is obtained by mycophenolic acid immunogen immunization animal.The present application has the advantages that: the mycophenolic acid immunogen of the present application is specific, and immunogenicity is high, and the prepared anti mycophenolic acid specific antibody is specific, and titer is high;Chemiluminescence immunoassay reagent containing the above anti mycophenolic acid specific antibody can determine the mycophenolic acid content in sample conveniently, quickly and accurately, and multiple samples can be simultaneously determined on full-automatic chemiluminescence immunoassay analyzer, realize the high-throughput rapid determination of mycophenolic acid, and the accuracy is high, and the specificity is strong, and accuracy and detection efficiency have been greatly improved.

The application provides a preparation method of sodium mycophenolate, which comprises the following steps: mixing mycophenolic acid crystals and first methanol, and obtaining first filtrate after first filtration treatment; mixing the first filtrate and a solid acid catalyst, and reacting at 50-55 DEG C until the content of mycophenolic acid is lower than 1%, and obtaining second filtrate after second filtration treatment; obtaining third filtrate after decolorization treatment and third filtration treatment of the second filtrate; obtaining first crystalline body after first crystallization treatment of the third filtrate; mixing the first crystalline body and second methanol, then adding sodium hydroxide and water, and reacting at 60-64 DEG C until the content of mycophenolate methyl ester is lower than 0.5%, and obtaining sodium mycophenolate concentrate after second crystallization treatment and reduced-pressure drying. By controlling the reaction degree of esterification and hydrolysis reactions and reasonably matching each step, the purity and yield of sodium mycophenolate can be improved.

This invention discloses the application of fluorescent carbon dots for mycophenolic acid detection, relating to the field of drug content detection technology. The carbon dot preparation method includes the following steps: adding β-cyclodextrin and L-phenylalanine to deionized water, ultrasonically mixing, and performing a hydrothermal reaction to obtain a reaction solution; cooling to room temperature, and then sequentially filtering, dialyzing, and freeze-drying to obtain fluorescent carbon dots for mycophenolic acid detection. This invention uses water as the reaction solvent and employs a one-step hydrothermal method to synthesize fluorescent carbon dots, which have been successfully applied to the sensing and determination of mycophenolic acid with good results. It offers advantages such as convenient construction, high sensitivity, good specificity, and ease of use. This invention solves the problems of complex operation, high cost, and low accuracy in existing methods for mycophenolic acid content detection.

The application discloses a fluorescence resonance energy transfer probe system and a method for high-sensitivity homogeneous immuno-detection of mycophenolic acid in whole blood. The application uses NaYbF4:Tm nanocrystals marked with BA monoclonal antibody on the surface as a donor probe, NaDyF4 nanocrystals marked with BA antigen on the surface as an acceptor probe, and constructs a fluorescence resonance energy transfer probe system. The probe system is used for qualitative and quantitative detection of mycophenolic acid in whole blood. The donor probe is incubated with a whole blood sample to be detected, and the fluorescence intensity emitted at 800 nm is tested. Then, the acceptor probe is added for joint incubation, and the fluorescence intensity emitted at 800 nm is tested. The ratio of the fluorescence intensity of the two times is compared with a control group or a standard curve, so that the mycophenolic acid in the whole blood can be qualitatively or quantitatively detected. The probe system can effectively overcome the background interference of the whole blood sample, and realize high-sensitivity homogeneous immuno-detection of BA in the whole blood.

The present application relates to the field of drug delivery technology and chronic kidney disease treatment, in particular, a kidney tissue targeted chronic kidney disease treatment drug nano delivery system, which solves the problems of poor targeting, low bioavailability, obvious side effects of existing chronic kidney disease treatment drugs, and insufficient responsiveness and poor safety of existing nano delivery systems. The system comprises a double-responsive nano carrier, an anti-kidney fibrosis drug and a targeting mediation layer. The double-responsive nano carrier is based on methoxy polyethylene glycol modified chitosan-polylactic acid copolymer, contains pH-sensitive hydrazone bonds and redox-sensitive disulfide bonds, and has a core-shell structure. The surface of the carrier is covalently connected with CD13 receptor affinity peptides, and is coated with a polylysine modified hyaluronic acid targeting mediation layer. The drug is pirfenidone or mycophenolate mofetil, which is covalently combined with the carrier through a disulfide bond. The system can realize precise enrichment of kidney lesions and double-responsive controlled drug release, improve the treatment effect, and reduce systemic and local toxicity.

Pharmaceutical formulations comprising mycophenolic acid or pharmaceutically acceptable salts or prodrugs thereof are disclosed. Methods of making the formulations and methods of using them to treat, manage or prevent disease and disorders associated with undesired immune response are also disclosed. The formulations include a core comprising mycophenolic acid, microcrystalline cellulose, povidone, and talc surrounded by a seal coat comprising a polymer, plasticizer and talc which is surrounded by a sustained release layer comprising cellulose, dibutyl sebacate, and talc.

Nanoparticles containing multiple therapeutic molecules arranged in the form of spherical micelles are described, which are suitable for topical delivery and release of mycophenolic acids and are therefore effective in treating autoimmune diseases, fibrosis and / or organ rejection, particularly lung diseases. Also described are pharmaceutical compositions containing nanoparticles in a pharmaceutically acceptable solvent and methods for producing said nanoparticles.

Provided herein are, inter alia, are methods for producing expanded population of mycophenolate resistant CAR-T cells. The methods provided herein are, inter alia, useful for producing an expanded population of mycophenolate resistant CAR-T cells for the treatment of cancer. Also provided herein are, inter alia, compositions including the expanded population of mycophenolate resistant CAR-T cells produced by the methods described herein.

The invention relates to the technical field of microbial secondary metabolites and application thereof in anti-influenza virus research, in particular to mycophenolic acid derivatives as well as a preparation method and application thereof. The mycophenolic acid derivatives are five compounds contained in the formula I, namely compounds 1-5. An in-vitro antiviral activity screening experiment result shows that the compounds 1-5 provided by the invention show remarkable inhibitory activity on influenza A viruses, the half inhibitory concentration reaches a micromole level, and the compounds 1-5 have the potential of being developed into anti-influenza virus drugs or lead compounds thereof. The compound not only can be used as an anti-influenza virus candidate drug for subsequent development, but also can be used as a lead compound for structure optimization and structure-function relationship research. The method for preparing the mycophenolic acid derivative is based on a microbial fermentation technology, and efficient and stable production of a target compound is realized by optimizing culture conditions, a fermentation process and metabolite extraction and purification steps of penicillium micranthum.